FastQCFastQC Report
Thu 26 May 2016
SRR936122_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936122_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818944
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56650.3114444424897083No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24690.13573809858907146No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC2300.024.6896364
ATCGGCC1505.5688724E-721.30242558
GGGCGGT3800.017.7456761
CGATTTG1400.003422354615.2105814
CGCGGAA3101.24400685E-814.88920159
TCGCGCT3201.8948413E-814.42113837
TAAGGCG5450.014.32875417
CTCGCGC3252.3261236E-814.19810236
CCTCGCG3554.9185473E-913.99697535
ATTCGCG3404.195863E-813.57432856
AATTCGC3404.195863E-813.57432855
ATCGTTC3404.2125976E-813.5702233
GCCTAGT4454.5474735E-1113.56035917
GACGTAT2102.3518338E-413.52460639
GCCTCGA4505.4569682E-1113.41042521
CGCGCCA3455.0713425E-813.37943947
CGAACTC4801.0913936E-1113.31108825
GCGAATA1600.00829526813.30998910
GTAACGG1600.00829526813.30998929
CGATCGT3506.157825E-813.1825031
Produced by FastQC (version 0.11.5)