##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936122_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1818944 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.82304457971218 40.0 36.0 41.0 30.0 41.0 2 36.69222636870624 39.0 36.0 41.0 30.0 41.0 3 36.5611635102565 39.0 36.0 41.0 30.0 41.0 4 36.453557668625315 39.0 36.0 41.0 30.0 41.0 5 36.37350572639949 39.0 36.0 41.0 30.0 41.0 6 36.28684115618733 39.0 36.0 41.0 30.0 41.0 7 36.13904716142993 39.0 35.0 40.0 29.0 41.0 8 36.0096737447662 39.0 35.0 40.0 29.0 41.0 9 36.1574858819183 39.0 35.0 41.0 30.0 41.0 10-11 36.37479768481053 39.0 36.0 41.0 30.0 41.0 12-13 36.28737360798354 39.5 35.0 41.0 30.0 41.0 14-15 36.13596955156399 39.0 35.0 41.0 29.0 41.0 16-17 35.98275950221667 39.0 35.0 41.0 28.5 41.0 18-19 35.81759801291299 39.0 35.0 41.0 28.0 41.0 20-21 35.60995720593927 39.0 35.0 41.0 27.0 41.0 22-23 35.33876084145526 39.0 35.0 41.0 26.0 41.0 24-25 35.104644782907 39.0 35.0 41.0 25.5 41.0 26-27 34.129307994088876 38.0 33.5 40.0 23.5 40.5 28-29 34.12442384152563 38.0 33.5 39.5 23.5 40.5 30-31 34.532371529854686 38.0 34.0 40.0 24.0 41.0 32-33 34.409318538668586 38.0 34.0 40.0 23.0 41.0 34-35 34.1549690369797 38.0 34.0 40.0 23.0 41.0 36-37 33.851123234140246 37.5 33.0 40.0 22.0 41.0 38-39 33.5410147316245 37.0 33.0 40.0 21.5 41.0 40-41 33.186962600277965 36.5 33.0 40.0 20.0 41.0 42-43 32.8183220593927 36.0 32.0 39.0 19.0 41.0 44-45 32.463557701611485 35.5 32.0 39.0 18.0 41.0 46-47 32.04829807844551 35.0 32.0 38.5 15.0 40.0 48-49 31.633205860103445 35.0 31.0 37.5 11.5 40.0 50-51 31.182319246771755 35.0 31.0 37.0 7.5 39.0 52-53 30.702538670789206 35.0 31.0 36.5 4.5 39.0 54-55 30.245774471341612 34.5 30.0 36.0 2.0 38.5 56-57 29.813157524365785 34.0 30.0 35.5 2.0 37.0 58-59 29.400238545001933 34.0 29.0 35.0 2.0 37.0 60-61 29.053395816473735 34.0 29.0 35.0 2.0 36.0 62-63 28.692853105977974 34.0 29.0 35.0 2.0 36.0 64-65 28.32879709325851 34.0 29.0 35.0 2.0 36.0 66-67 27.991868083899227 34.0 28.5 35.0 2.0 35.0 68-69 27.641894142975268 34.0 27.0 35.0 2.0 35.0 70-71 27.2096452667042 33.5 26.5 35.0 2.0 35.0 72-73 26.74859368952535 33.0 25.5 35.0 2.0 35.0 74-75 26.20853225827733 33.0 25.0 35.0 2.0 35.0 76-77 24.46230972476338 31.0 19.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9246819178308598 1101 2 0.9815512732045164 1101 3 1.0383995449400985 1101 4 0.7931063552624238 1101 5 0.882476361447047 1101 6 0.865089569825372 1101 7 0.6944692770231029 1101 8 0.8070610517620267 1101 9 0.7904183940064655 1101 10-11 0.7744935991622341 1101 12-13 0.8471380351209419 1101 14-15 0.92271324894228 1101 16-17 0.8314233882836746 1101 18-19 0.8877217577968182 1101 20-21 1.0352069153354364 1101 22-23 1.0342545719161578 1101 24-25 1.0206390662067975 1101 26-27 1.0197759460717464 1101 28-29 1.112195718848973 1101 30-31 0.95725287104937 1101 32-33 1.0767097780463928 1101 34-35 1.1767806859260546 1101 36-37 1.0175261017295512 1101 38-39 1.1180463127835694 1101 40-41 1.0158035027456478 1101 42-43 1.0119366806303773 1101 44-45 1.0175973219626258 1101 46-47 1.1743853960789963 1101 48-49 1.0151435947193725 1101 50-51 0.9601420990505076 1101 52-53 1.0349705517342827 1101 54-55 1.2164034180760588 1101 56-57 1.1114897607467569 1101 58-59 1.0424309223804862 1101 60-61 1.007521566632974 1101 62-63 1.0791728074223172 1101 64-65 1.1144532574918955 1101 66-67 1.2540762586305192 1101 68-69 1.0788351412151016 1101 70-71 1.1443085432710092 1101 72-73 1.1296688591386719 1101 74-75 1.3021801748988757 1101 76-77 1.4257826869917771 1102 1 0.4983964700632697 1102 2 0.6148115590158127 1102 3 0.6273992054234441 1102 4 0.4561960939717764 1102 5 0.37772484305313725 1102 6 0.42226040157257927 1102 7 0.5449482012697331 1102 8 0.6270417781904882 1102 9 0.49947320022476305 1102 10-11 0.7373851150183484 1102 12-13 0.8678279741108312 1102 14-15 0.8970042935904345 1102 16-17 0.9334001296782972 1102 18-19 1.0466995726127806 1102 20-21 1.0873741632957703 1102 22-23 0.9401981709984355 1102 24-25 1.1324497873881754 1102 26-27 1.1039006275605416 1102 28-29 1.1545529932504408 1102 30-31 1.2576462302408373 1102 32-33 1.1933628983416043 1102 34-35 1.0960580298769997 1102 36-37 1.1063405713119465 1102 38-39 1.0290586554141754 1102 40-41 1.064587587302448 1102 42-43 1.0450944255539127 1102 44-45 0.9199537047042057 1102 46-47 1.1104979647110937 1102 48-49 0.8311427167773253 1102 50-51 0.7828938300710178 1102 52-53 0.950780710484036 1102 54-55 1.081375245052886 1102 56-57 1.0931369995161297 1102 58-59 0.9894063091379657 1102 60-61 0.7908825071283943 1102 62-63 0.7112893679471668 1102 64-65 0.7213289841089718 1102 66-67 0.8010941707059942 1102 68-69 0.8507365373487232 1102 70-71 0.8767162650456903 1102 72-73 0.7963355258053397 1102 74-75 0.5164769124525002 1102 76-77 0.36516181734902275 1103 1 0.21937041443958094 1103 2 0.13395623509943988 1103 3 0.11629036528476178 1103 4 0.34908151584275515 1103 5 0.13395676989754435 1103 6 0.18691801462915691 1103 7 0.1848033889277545 1103 8 0.071225060805439 1103 9 0.1689698770351029 1103 10-11 0.25420564006717683 1103 12-13 0.3324819914610586 1103 14-15 0.3347419887048204 1103 16-17 0.386822106635492 1103 18-19 0.46801491984447097 1103 20-21 0.23175312803513037 1103 22-23 0.06653009632886864 1103 24-25 0.2261811464894734 1103 26-27 0.2928527251820796 1103 28-29 0.19408551357565074 1103 30-31 0.4462342152827006 1103 32-33 0.4995346444165989 1103 34-35 0.4470546082785347 1103 36-37 0.5217137299317969 1103 38-39 0.3395554684828639 1103 40-41 0.5083000216334952 1103 42-43 0.4514323311117465 1103 44-45 0.4450818253988231 1103 46-47 0.29840806448248003 1103 48-49 0.4205189185002389 1103 50-51 0.3975749098427066 1103 52-53 0.4582743517612329 1103 54-55 0.47476501205111177 1103 56-57 0.5285876361453994 1103 58-59 0.4414874661227195 1103 60-61 0.3253759288782554 1103 62-63 0.2526064941072548 1103 64-65 0.24822884179469895 1103 66-67 0.409557138715563 1103 68-69 0.4089718403721463 1103 70-71 0.1418526747896749 1103 72-73 0.13034636591374138 1103 74-75 0.08983083593587082 1103 76-77 0.2588369698826689 1104 1 0.46473279205385865 1104 2 0.30858256034131415 1104 3 0.632048432604158 1104 4 0.46056260036856855 1104 5 0.47341421732237166 1104 6 0.48444422979629564 1104 7 0.32792516634505375 1104 8 0.4686672715695863 1104 9 0.3427474681364302 1104 10-11 0.4957755085568394 1104 12-13 0.4741097940217429 1104 14-15 0.7271287275959537 1104 16-17 0.6620466011607249 1104 18-19 0.5960970124231082 1104 20-21 0.6784248156546226 1104 22-23 0.5319738713129354 1104 24-25 0.6657964642578591 1104 26-27 0.3506723764189772 1104 28-29 0.4684880684681829 1104 30-31 0.6503330508078733 1104 32-33 0.5791824052647598 1104 34-35 0.6113092864547696 1104 36-37 0.6059100395314729 1104 38-39 0.5158343631007867 1104 40-41 0.6194088797529531 1104 42-43 0.7609653824992648 1104 44-45 0.5626525049654774 1104 46-47 0.4427855628892914 1104 48-49 0.4751065727496986 1104 50-51 0.40221398058020696 1104 52-53 0.3515329984213338 1104 54-55 0.19951806807336325 1104 56-57 0.21196635116770324 1104 58-59 0.15125732302556472 1104 60-61 0.3144727367109432 1104 62-63 0.217329714082922 1104 64-65 0.18865740908512407 1104 66-67 0.16882876260757484 1104 68-69 -0.22483413842398292 1104 70-71 -0.03303000871649786 1104 72-73 -0.22035753304923844 1104 74-75 -0.21044724760505673 1104 76-77 -0.29242092363450567 1105 1 -0.1145772022933258 1105 2 0.17886016098512414 1105 3 -0.2065086510993055 1105 4 0.024990788710397283 1105 5 -0.07883953010531997 1105 6 0.06531849499972964 1105 7 0.049120862407299626 1105 8 -0.08297246299864014 1105 9 -0.18626916451123066 1105 10-11 -0.025275316670878567 1105 12-13 0.25760029701105225 1105 14-15 0.1384752257734121 1105 16-17 0.06734207918574242 1105 18-19 0.16308254343980622 1105 20-21 0.11483793631580141 1105 22-23 0.20630792493950167 1105 24-25 0.20125048569400406 1105 26-27 -0.10981290258791176 1105 28-29 0.05187616636494141 1105 30-31 0.44494679355737077 1105 32-33 0.5160588202093592 1105 34-35 0.40377050339762377 1105 36-37 0.25701846501315373 1105 38-39 0.24285587165102385 1105 40-41 0.3489570999510505 1105 42-43 0.36651468035258006 1105 44-45 0.16004872843830498 1105 46-47 0.02566337572257993 1105 48-49 -0.031041213601671558 1105 50-51 0.009807203326253955 1105 52-53 0.12877337958269308 1105 54-55 -0.11361352281864256 1105 56-57 -0.08558901441360334 1105 58-59 0.05483955201755464 1105 60-61 0.012778353605899184 1105 62-63 -0.16290297033674506 1105 64-65 -0.037221005230144044 1105 66-67 0.05004372837044002 1105 68-69 -0.1043598726636219 1105 70-71 -0.17702403009695544 1105 72-73 -0.6957837916363694 1105 74-75 -0.8286934542308302 1105 76-77 -0.7905343308350261 1106 1 0.0019222807804766262 1106 2 -0.09011659668272642 1106 3 -0.19823715318092638 1106 4 -0.058958296605162275 1106 5 -0.2836061123033673 1106 6 -0.24238699758715399 1106 7 -0.5798635931502858 1106 8 -0.3938949132084346 1106 9 -0.23654405520323252 1106 10-11 -0.3588264400943899 1106 12-13 0.012663152928148236 1106 14-15 -0.025659701806006296 1106 16-17 0.049177876545250854 1106 18-19 0.04833748682230521 1106 20-21 -0.0164903365940674 1106 22-23 0.07525169165135992 1106 24-25 0.06810567372549059 1106 26-27 -0.0547410861642561 1106 28-29 0.10668578614757251 1106 30-31 0.2794396187032717 1106 32-33 0.19480276762476478 1106 34-35 0.062495525137038044 1106 36-37 0.26896241297161083 1106 38-39 0.4062208405505956 1106 40-41 0.2914281021905012 1106 42-43 0.2825447211296819 1106 44-45 0.08459786901033084 1106 46-47 0.18819932805811135 1106 48-49 0.1877193718452439 1106 50-51 0.14294471791253116 1106 52-53 0.1671433548258996 1106 54-55 0.05742438894887769 1106 56-57 0.010366979995584558 1106 58-59 0.044503234337728514 1106 60-61 0.02497448551813264 1106 62-63 -0.006116336968773339 1106 64-65 0.1613770827878227 1106 66-67 -0.05500480431267363 1106 68-69 0.11276525062819331 1106 70-71 -0.15177542695168 1106 72-73 -0.37354802305910084 1106 74-75 -0.1356934766320954 1106 76-77 -0.177102803021139 1107 1 -0.4630183749369934 1107 2 -0.4879616515283445 1107 3 -0.2764013156353542 1107 4 -0.42332525308964364 1107 5 -0.27274313986798404 1107 6 -0.5686058166997157 1107 7 -0.4982456609166874 1107 8 -0.5461504627565361 1107 9 -0.39561410163184973 1107 10-11 -0.2970665650209696 1107 12-13 -0.19465383835942163 1107 14-15 -0.4737860109180829 1107 16-17 -0.4267760267229832 1107 18-19 -0.36222036318202555 1107 20-21 -0.2899216642235558 1107 22-23 -0.1698216026381587 1107 24-25 -0.14977405950101286 1107 26-27 -0.4529685432183399 1107 28-29 -0.4280901136886328 1107 30-31 -0.04078178450181014 1107 32-33 -0.004121198602348386 1107 34-35 -0.28005580100888494 1107 36-37 -0.1890687847818029 1107 38-39 -0.009680568742219009 1107 40-41 -0.14953152272281045 1107 42-43 -0.20503727872515753 1107 44-45 -0.19225285696236227 1107 46-47 -0.1413725445328886 1107 48-49 -0.1693638776910653 1107 50-51 -0.26620470255308604 1107 52-53 -0.2611632652405582 1107 54-55 -0.0896420329718488 1107 56-57 -0.12806277933722043 1107 58-59 -0.07837640776450172 1107 60-61 -0.16982043211351439 1107 62-63 -0.09727482062251624 1107 64-65 -0.13998495330569938 1107 66-67 -0.16204576898938683 1107 68-69 -0.14446178341743376 1107 70-71 -0.25128041432697046 1107 72-73 -0.323334392594564 1107 74-75 -0.42225789014189274 1107 76-77 -0.4158898014739414 1108 1 0.26604556196659246 1108 2 0.32293033961383344 1108 3 0.2512326199503505 1108 4 0.2989626283895461 1108 5 0.12573288850526154 1108 6 0.11499410259921206 1108 7 0.2193630058237659 1108 8 0.2907139446620519 1108 9 0.17260077312014488 1108 10-11 0.2224855017980687 1108 12-13 0.406730203153991 1108 14-15 0.3220172015033711 1108 16-17 0.19836084995848324 1108 18-19 0.13634490079583372 1108 20-21 0.3106068132131625 1108 22-23 0.17904558166940632 1108 24-25 0.12408379864543662 1108 26-27 -0.00537794977895345 1108 28-29 0.179220752216402 1108 30-31 0.17900152889248488 1108 32-33 0.3957069346485156 1108 34-35 0.35943522129821304 1108 36-37 0.35064151163178536 1108 38-39 0.3368563569750975 1108 40-41 0.399187981031929 1108 42-43 0.30870045062526685 1108 44-45 0.2765748453399439 1108 46-47 0.17412372032827506 1108 48-49 0.25538674362578107 1108 50-51 0.2532875019222054 1108 52-53 0.3783538431760256 1108 54-55 0.41560052110254375 1108 56-57 0.47481254323335875 1108 58-59 0.45566521119072334 1108 60-61 0.4761821656407861 1108 62-63 0.43333157436460823 1108 64-65 0.5049589706060935 1108 66-67 0.39854306537166195 1108 68-69 0.41936866471177225 1108 70-71 0.2867751481815297 1108 72-73 0.2739737320071747 1108 74-75 0.2132526632726588 1108 76-77 0.2597642587159079 1109 1 -0.031247648961759467 1109 2 -0.15243473984573086 1109 3 -0.23023385881306524 1109 4 -0.17307406334246167 1109 5 -0.13872050796515367 1109 6 -0.32794873621818965 1109 7 -0.29381527259945983 1109 8 -0.10410224696355641 1109 9 -0.005923789476490526 1109 10-11 -0.19093043356875938 1109 12-13 -0.13058788957835077 1109 14-15 0.01408255493063848 1109 16-17 -0.04876472130363396 1109 18-19 -0.1653006912408017 1109 20-21 -0.19369214344755648 1109 22-23 -0.21727066129724193 1109 24-25 -0.20676562030789114 1109 26-27 -0.2967581607087979 1109 28-29 0.09206434656561413 1109 30-31 0.15687919434829922 1109 32-33 0.015607615773021166 1109 34-35 0.06991132390291455 1109 36-37 0.06480122245522324 1109 38-39 0.08580222863438536 1109 40-41 -0.006563406292336538 1109 42-43 -0.003955786120926064 1109 44-45 0.012983234317538006 1109 46-47 -0.02049603600266181 1109 48-49 0.23399419596417914 1109 50-51 0.09827936920337521 1109 52-53 0.05352174326784365 1109 54-55 0.11510943286589637 1109 56-57 0.14487327447862342 1109 58-59 0.20816105554253994 1109 60-61 0.24440131599861203 1109 62-63 0.15446816088468296 1109 64-65 0.3087184742795799 1109 66-67 0.4593851775272846 1109 68-69 0.3692429907675745 1109 70-71 0.4664937992663134 1109 72-73 0.27895738472147613 1109 74-75 0.15961883354336592 1109 76-77 0.27661543070543004 1110 1 -0.37168628229256484 1110 2 -0.160058147574631 1110 3 -0.5042078320696461 1110 4 -0.4926093144006316 1110 5 -0.3964973556589655 1110 6 -0.5138864400224392 1110 7 -0.47944681314817217 1110 8 -0.7592781403338904 1110 9 -0.7489510503330337 1110 10-11 -0.5030901028665866 1110 12-13 -0.5520426810476309 1110 14-15 -0.5012607248862366 1110 16-17 -0.5478621417061973 1110 18-19 -0.6263295201806827 1110 20-21 -0.8548588605203165 1110 22-23 -0.7084901112640623 1110 24-25 -0.8690261256779266 1110 26-27 -0.7099792246261032 1110 28-29 -0.6090801822712066 1110 30-31 -0.5215000324174852 1110 32-33 -0.4982643317320168 1110 34-35 -0.4987110991155248 1110 36-37 -0.43331234748573166 1110 38-39 -0.4803323312630994 1110 40-41 -0.4152209750178244 1110 42-43 -0.39812406411028434 1110 44-45 -0.43165009361684525 1110 46-47 -0.44540648755046774 1110 48-49 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8 -0.17666850903935227 2314 9 -0.2269059544504799 2314 10-11 -0.32821121137406095 2314 12-13 -0.3283993832617824 2314 14-15 -0.2757291864614757 2314 16-17 -0.3895858756261461 2314 18-19 -0.3567522641910088 2314 20-21 -0.31040319125533244 2314 22-23 -0.13693997018997806 2314 24-25 -0.09285987346617475 2314 26-27 -0.061958804751405694 2314 28-29 -0.32082579462834104 2314 30-31 -0.3721194432538866 2314 32-33 -0.4200828722131078 2314 34-35 -0.4530617997037254 2314 36-37 -1.0070002390260768 2314 38-39 -0.5096795647765546 2314 40-41 -0.35267004488535036 2314 42-43 -0.31371179305676833 2314 44-45 -0.31766762027745443 2314 46-47 -0.15359858740222165 2314 48-49 -0.20912631431572848 2314 50-51 -0.14282974647658264 2314 52-53 -0.12178108061127446 2314 54-55 -0.2444589347338102 2314 56-57 -0.09709972527366872 2314 58-59 -0.23232073786840957 2314 60-61 -0.27260078952529554 2314 62-63 -0.22798663227855798 2314 64-65 -0.20536871918091038 2314 66-67 -0.082410710508384 2314 68-69 -0.002602439007013402 2314 70-71 -0.19183912121919278 2314 72-73 -0.13477558530577127 2314 74-75 0.07191509314207778 2314 76-77 0.18842122708888098 2315 1 -1.0104009550973174 2315 2 -0.9036543744320653 2315 3 -0.6316434262078445 2315 4 -0.6792693353453103 2315 5 -0.7972351179881869 2315 6 -0.747556225140869 2315 7 -1.0685755357454667 2315 8 -1.0522084387229285 2315 9 -0.6224115630223892 2315 10-11 -0.8579496175351196 2315 12-13 -0.7672726462786912 2315 14-15 -0.7588398511191059 2315 16-17 -0.6541505834781489 2315 18-19 -0.5469774441675739 2315 20-21 -0.5328563136393925 2315 22-23 -0.764548374676842 2315 24-25 -0.8667151388269687 2315 26-27 -0.6981491613561204 2315 28-29 -0.7927306163259829 2315 30-31 -0.9205862940826179 2315 32-33 -0.6910158204803096 2315 34-35 -0.7195514196601991 2315 36-37 -0.8232504483011454 2315 38-39 -0.812355774386468 2315 40-41 -0.8624193333501111 2315 42-43 -0.9577533132308886 2315 44-45 -0.8997599524388491 2315 46-47 -0.7382143583715823 2315 48-49 -0.6728551775335454 2315 50-51 -0.7496491839451913 2315 52-53 -0.7463369989102837 2315 54-55 -0.9019974413469001 2315 56-57 -0.9423776425681645 2315 58-59 -0.8562032089884504 2315 60-61 -0.8281905266758045 2315 62-63 -0.7703514405825942 2315 64-65 -0.5721642992914049 2315 66-67 -0.7842908377476299 2315 68-69 -0.7555724708168228 2315 70-71 -0.7191813278155443 2315 72-73 -0.48811812674223276 2315 74-75 -0.5475742756843083 2315 76-77 -0.6513351767282991 2316 1 -1.918291566336464 2316 2 -1.4518514398275855 2316 3 -1.8047894251325687 2316 4 -2.9360269395232166 2316 5 -2.2319472690877618 2316 6 -2.1557643015280092 2316 7 -1.9332703680491719 2316 8 -1.5799620857680807 2316 9 -1.8364586926486908 2316 10-11 -1.71449848319088 2316 12-13 -2.3539688738613123 2316 14-15 -3.690247782537412 2316 16-17 -2.8331764888186584 2316 18-19 -2.435921472549005 2316 20-21 -2.172806455242217 2316 22-23 -2.5102246699669237 2316 24-25 -2.2031622588624558 2316 26-27 -2.358072748394136 2316 28-29 -2.871569523858554 2316 30-31 -2.8224612058978487 2316 32-33 -3.0782607071473294 2316 34-35 -2.9287313267215254 2316 36-37 -2.7491094255331063 2316 38-39 -2.971055264690065 2316 40-41 -2.739260953976263 2316 42-43 -2.801241496703149 2316 44-45 -2.7852229305155376 2316 46-47 -2.7892117799182685 2316 48-49 -2.6421325281819854 2316 50-51 -2.8017985405768258 2316 52-53 -2.714298379286557 2316 54-55 -2.7813937688030954 2316 56-57 -2.5185275488271834 2316 58-59 -2.7805942845520732 2316 60-61 -2.6656261229800187 2316 62-63 -2.605049044078072 2316 64-65 -2.6474969114693394 2316 66-67 -2.8215225601009557 2316 68-69 -2.8294772967481663 2316 70-71 -2.6278344778905662 2316 72-73 -2.530599320909179 2316 74-75 -2.8286102403126456 2316 76-77 -3.0207771158801187 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38802.0 3 3834.0 4 4845.0 5 6071.0 6 6762.0 7 7242.0 8 8562.0 9 8936.0 10 10755.0 11 11680.0 12 9640.0 13 8230.0 14 7962.0 15 8487.0 16 8657.0 17 8868.0 18 9634.0 19 10086.0 20 10904.0 21 11834.0 22 12891.0 23 15019.0 24 17315.0 25 20320.0 26 23900.0 27 28634.0 28 33528.0 29 40060.0 30 48156.0 31 57782.0 32 70385.0 33 88153.0 34 118174.0 35 158758.0 36 224310.0 37 307164.0 38 280397.0 39 71733.0 40 474.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.627791803379633 26.703230970874642 28.77910556733315 21.889871658412577 2 22.253488292817565 27.078982932684113 28.58723178714734 22.080296987350987 3 22.378666666666668 26.980178694158074 28.302405498281786 22.338749140893473 4 22.10280959670608 27.10081908079955 28.585183083474096 22.21118823902027 5 22.359426075146878 27.0592536997479 28.375300007422865 22.20602021768236 6 22.652886959074905 26.902210519196817 28.433513149133482 22.0113893725948 7 22.47231045936897 26.727561753325414 28.448291504618545 22.351836282687074 8 22.33058235690991 27.081529087602902 28.20105497169018 22.38683358379701 9 22.34704083658164 26.909133698331555 28.28257834567498 22.461247119411823 10-11 22.781462124696773 26.91383577127093 28.034380680085746 22.270321423946548 12-13 22.782877896826378 26.75269508986382 28.010926077142393 22.453500936167405 14-15 22.42818389146322 26.88436188017233 28.163658475972227 22.52379575239222 16-17 22.86236441848395 26.66359509839282 28.18696284208767 22.287077641035566 18-19 22.859873935256562 26.674750982544037 27.883687019862013 22.58168806233739 20-21 22.754445254212268 26.817063552173163 27.87621603774648 22.552275155868088 22-23 22.76589684685824 26.967785674775413 27.47842682716279 22.787890651203558 24-25 22.863550532339 26.993040716427892 27.376637130769204 22.766771620463903 26-27 22.718758994996204 27.091973226217675 27.320403173771435 22.868864605014682 28-29 23.010338673478476 26.919395294265126 27.28141930787408 22.788846724382314 30-31 22.87964266726193 26.82387210531727 27.3565833465746 22.939901880846204 32-33 22.65593643232354 27.21438915848264 27.33474416451413 22.794930244679694 34-35 22.769411873377326 26.91298127623356 27.399054739553037 22.918552110836078 36-37 22.70888876548498 26.857331209564215 27.70520772452288 22.728572300427924 38-39 22.512838404224638 26.882225941068295 27.70761182175162 22.897323832955447 40-41 22.60427737801797 26.848775327412355 27.539421717670177 23.0075255768995 42-43 22.44360787530898 27.104647540801075 27.316945363689925 23.13479922020002 44-45 22.43542035706617 26.832969826404707 27.610149747625474 23.121460068903648 46-47 22.47125646763844 26.758977714483663 27.543617038825076 23.226148779052824 48-49 22.546041575083873 26.664841196463758 27.565067428305618 23.22404980014675 50-51 22.37123031208387 26.945379067397063 27.422373594937945 23.261017025581125 52-53 22.509767518083898 26.715636470423576 27.454115556015058 23.32048045547747 54-55 22.44831634602959 26.739070065410512 27.52809297696197 23.284520611597927 56-57 22.393337827420456 26.85504809516321 27.488170030450554 23.263444046965777 58-59 22.407904923614257 26.89127843295958 27.42480194252484 23.27601470090132 60-61 22.451122415903008 26.621859293373827 27.397373638340934 23.52964465238223 62-63 22.51658433271002 26.588272752093268 27.467014864104293 23.42812805109242 64-65 22.416348629104828 26.51172662984112 27.49964326454594 23.57228147650811 66-67 22.356196442082666 26.51114158645809 27.645810605127487 23.486851366331756 68-69 22.36815043256953 26.245465883869436 27.746591026574485 23.639792656986554 70-71 22.294834076519223 26.118704934070387 27.792337736525223 23.794123252885168 72-73 22.38770252494995 26.01069240413503 27.715585130758598 23.886019940156427 74-75 22.273565331929216 25.997366728454463 27.72863548006037 24.000432459555952 76-77 22.166571072130548 25.837428768315984 27.986393940269235 24.009606219284237 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5964.0 1 3574.5 2 1132.5 3 1064.0 4 1048.0 5 1112.0 6 1178.5 7 1199.0 8 1255.5 9 1294.0 10 1389.5 11 1451.0 12 1524.0 13 1631.0 14 1726.0 15 1952.0 16 2284.5 17 2486.0 18 2702.0 19 3214.5 20 3843.0 21 4817.0 22 5459.0 23 6171.5 24 7882.5 25 10264.0 26 11647.0 27 13593.0 28 18125.5 29 22584.0 30 24456.0 31 28097.0 32 35065.5 33 42052.5 34 49072.5 35 52433.0 36 55853.5 37 62676.5 38 67569.5 39 69060.0 40 71527.5 41 76462.0 42 81916.0 43 84903.0 44 86498.5 45 89085.0 46 91195.0 47 91649.0 48 90984.0 49 90581.0 50 88306.0 51 84416.5 52 82399.0 53 80601.5 54 74794.0 55 66373.5 56 57263.5 57 52564.0 58 49605.5 59 42582.5 60 35073.0 61 31628.0 62 27871.5 63 20767.0 64 14966.0 65 12513.0 66 10896.5 67 8295.5 68 6193.0 69 4386.0 70 3697.0 71 3174.5 72 2180.0 73 1500.0 74 1292.0 75 1108.0 76 749.0 77 438.5 78 303.0 79 262.5 80 184.5 81 127.0 82 93.0 83 79.0 84 69.0 85 48.5 86 37.5 87 37.0 88 40.0 89 36.0 90 22.0 91 15.0 92 19.0 93 20.5 94 18.5 95 18.0 96 17.0 97 14.0 98 11.0 99 32.0 100 53.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002638893775729214 2 0.008026635234509694 3 0.010665529010238909 4 0.017592625171528098 5 0.013139491924985047 6 0.012699676295696846 7 0.016328155237324515 8 0.01742769431054502 9 0.016767970866612716 10-11 0.020039099609443722 12-13 0.019736726364308083 14-15 0.00725695788325534 16-17 0.006157418810034833 18-19 0.010775482917560959 20-21 0.009813386228493017 22-23 0.013744238415256323 24-25 0.02012156503993526 26-27 0.012342326096900181 28-29 0.007806727419865592 30-31 0.007421888744238416 32-33 0.008686358678441997 34-35 0.010225713380950706 36-37 0.009071197354069175 38-39 0.01536605854825657 40-41 0.011710091129798388 42-43 0.014981219872629395 44-45 0.017455182787375532 46-47 0.015063685303120931 48-49 0.012892095633510433 50-51 0.026086564512156504 52-53 0.029934951268428274 54-55 0.023585113120579853 56-57 0.014184054044544528 58-59 0.015393547025087084 60-61 0.018939560536223216 62-63 0.02394246331937652 64-65 0.020313984377748846 66-67 0.019351887688680906 68-69 0.02792829245980085 70-71 0.054014856971957355 72-73 0.06624722916153548 74-75 0.07878197459624925 76-77 0.0754008919460962 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1818944.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.19809547453467 #Duplication Level Percentage of deduplicated Percentage of total 1 71.21414131616469 36.46028406242003 2 13.841833759088424 14.173510526808922 3 5.829231208984877 8.953366079422345 4 3.053229278145257 6.2527809635250176 5 1.7057344185660888 4.366517680797324 6 1.1006724955127394 3.3811401306873337 7 0.7193107860349182 2.577913960949482 8 0.4961980114131604 2.032351453008418 9 0.36176746863635256 1.6669624858942234 >10 1.561892107386763 13.47471453210572 >50 0.08034678512636172 2.8154731928696757 >100 0.034135187045627735 3.002953380774916 >500 0.001291866767022813 0.3937050053617498 >1k 1.0765556391856771E-4 0.1360853504463335 >5k 1.0765556391856771E-4 0.3122411949285051 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5665 0.3114444424897083 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2469 0.13573809858907146 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.4976953661025295E-5 0.0 2 0.0 0.0 0.0 4.3981562928820236E-4 0.0 3 0.0 0.0 0.0 9.895851658984554E-4 0.0 4 0.0 0.0 0.0 0.0023640090074240876 0.0 5 0.0 0.0 0.0 0.004728018014848175 0.0 6 0.0 0.0 0.0 0.008356496956475846 0.0 7 0.0 0.0 0.0 0.015943316561697337 0.0 8 0.0 0.0 0.0 0.025344375637732664 0.0 9 0.0 0.0 0.0 0.037494282396819255 0.0 10 0.0 0.0 0.0 0.05250299074627916 0.0 11 0.0 0.0 0.0 0.07201980929594314 0.0 12 0.0 0.0 0.0 0.09923340135815066 0.0 13 0.0 0.0 0.0 0.12919584110340945 0.0 14 0.0 0.0 0.0 0.16097252031948206 0.0 15 0.0 0.0 0.0 0.1953880933112839 0.0 16 0.0 0.0 0.0 0.23051836670067907 0.0 17 0.0 0.0 0.0 0.2647140318778368 0.0 18 0.0 0.0 0.0 0.3001191900355371 0.0 19 0.0 0.0 0.0 0.33689877203476304 0.0 20 0.0 0.0 0.0 0.38170498926849866 0.0 21 0.0 0.0 0.0 0.4332733718025404 0.0 22 0.0 0.0 0.0 0.4851166391048872 0.0 23 0.0 0.0 0.0 0.5381144224341157 0.0 24 0.0 0.0 0.0 0.5899576897364625 0.0 25 0.0 0.0 0.0 0.6383374089581647 0.0 26 0.0 0.0 0.0 0.6904555610288167 0.0 27 0.0 0.0 0.0 0.7467519615777066 0.0 28 0.0 0.0 0.0 0.8007393300728335 0.0 29 0.0 0.0 0.0 0.8599495091657577 0.0 30 0.0 0.0 0.0 0.9221834207100383 0.0 31 0.0 0.0 0.0 0.9982715245768974 0.0 32 0.0 0.0 0.0 1.077713222617079 0.0 33 0.0 0.0 0.0 1.158474367545125 0.0 34 0.0 0.0 0.0 1.2413796136659512 0.0 35 0.0 0.0 0.0 1.3295076703845747 0.0 36 0.0 0.0 0.0 1.416426234122656 5.4976953661025295E-5 37 0.0 0.0 0.0 1.5062585764047711 5.4976953661025295E-5 38 0.0 0.0 0.0 1.5958160339185814 5.4976953661025295E-5 39 0.0 0.0 0.0 1.6941698040181556 5.4976953661025295E-5 40 0.0 0.0 0.0 1.7987909468350867 5.4976953661025295E-5 41 0.0 0.0 0.0 1.9142425495232398 5.4976953661025295E-5 42 0.0 0.0 0.0 2.0374459026775975 5.4976953661025295E-5 43 0.0 0.0 0.0 2.1721394391471094 5.4976953661025295E-5 44 0.0 0.0 0.0 2.31425486436086 5.4976953661025295E-5 45 0.0 0.0 0.0 2.4617030540797296 5.4976953661025295E-5 46 0.0 0.0 0.0 2.618662256781957 5.4976953661025295E-5 47 0.0 0.0 0.0 2.7736422891523875 5.4976953661025295E-5 48 0.0 0.0 0.0 2.937968403645192 5.4976953661025295E-5 49 0.0 0.0 0.0 3.1084519369480312 5.4976953661025295E-5 50 0.0 0.0 0.0 3.287346944161008 5.4976953661025295E-5 51 0.0 0.0 0.0 3.479381443298969 5.4976953661025295E-5 52 0.0 0.0 0.0 3.6819165405861862 5.4976953661025295E-5 53 0.0 0.0 0.0 3.8954470286056084 5.4976953661025295E-5 54 0.0 0.0 0.0 4.127064934379508 5.4976953661025295E-5 55 0.0 0.0 0.0 4.361211780021815 5.4976953661025295E-5 56 0.0 0.0 0.0 4.603495214805953 5.4976953661025295E-5 57 0.0 5.4976953661025295E-5 0.0 4.850011875021991 5.4976953661025295E-5 58 0.0 5.4976953661025295E-5 0.0 5.115330653390099 5.4976953661025295E-5 59 0.0 5.4976953661025295E-5 0.0 5.383783118116885 5.4976953661025295E-5 60 0.0 5.4976953661025295E-5 0.0 5.661691618873368 5.4976953661025295E-5 61 0.0 5.4976953661025295E-5 5.4976953661025295E-5 5.962910347982126 5.4976953661025295E-5 62 0.0 5.4976953661025295E-5 5.4976953661025295E-5 6.274244836564512 5.4976953661025295E-5 63 0.0 5.4976953661025295E-5 5.4976953661025295E-5 6.609494299989445 5.4976953661025295E-5 64 0.0 5.4976953661025295E-5 5.4976953661025295E-5 6.951066113085394 5.4976953661025295E-5 65 0.0 5.4976953661025295E-5 5.4976953661025295E-5 7.311440044333415 5.4976953661025295E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAC 230 0.0 24.689636 4 ATCGGCC 150 5.5688724E-7 21.302425 58 GGGCGGT 380 0.0 17.745676 1 CGATTTG 140 0.0034223546 15.210581 4 CGCGGAA 310 1.24400685E-8 14.889201 59 TCGCGCT 320 1.8948413E-8 14.421138 37 TAAGGCG 545 0.0 14.328754 17 CTCGCGC 325 2.3261236E-8 14.198102 36 CCTCGCG 355 4.9185473E-9 13.996975 35 ATTCGCG 340 4.195863E-8 13.574328 56 AATTCGC 340 4.195863E-8 13.574328 55 ATCGTTC 340 4.2125976E-8 13.570223 3 GCCTAGT 445 4.5474735E-11 13.560359 17 GACGTAT 210 2.3518338E-4 13.524606 39 GCCTCGA 450 5.4569682E-11 13.410425 21 CGCGCCA 345 5.0713425E-8 13.379439 47 CGAACTC 480 1.0913936E-11 13.311088 25 GCGAATA 160 0.008295268 13.309989 10 GTAACGG 160 0.008295268 13.309989 29 CGATCGT 350 6.157825E-8 13.182503 1 >>END_MODULE