FastQCFastQC Report
Thu 26 May 2016
SRR936122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936122_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818944
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47290.25998601386298864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36190.19896159529925053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23320.12820625593751098No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA7850.048.53006100-101
CCTGTCG6800.036.76048396-97
ACGTATC3050.035.129597100-101
GGTATCA55200.034.926191
GCGTATC1650.032.468266100-101
CGGTATC8150.031.771093100-101
CCGTATC4050.030.864891100-101
CGAGCCG3700.030.56276596-97
TCGTATC3350.030.206795100-101
CGAGTAC6850.029.110842100-101
CGTACAT8900.029.093754100-101
GTATCAA100850.029.0291611
CGGCCTA1550.028.802492100-101
TACGCAG5450.027.85122100-101
CGAGCGT2050.027.58103496-97
ACGGACG2700.027.55415296-97
AGTATCA19550.026.184978100-101
ATACACG6600.026.15139496-97
CCGAGCG5200.025.75253396-97
AGGTATC16950.025.6362100-101
Produced by FastQC (version 0.11.5)