FastQCFastQC Report
Thu 26 May 2016
SRR936121_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936121_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26252
Sequences flagged as poor quality0
Sequence length77
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8633.287368581441414No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7232.7540758799329574No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACATT150.002082299270.9828622
AGAGGCC200.006458979653.338744
AAGTAGA200.006508010453.2371448
CCTACAT200.006508010453.2371421
CCCTACA200.006508010453.2371420
Produced by FastQC (version 0.11.5)