##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936121_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26252 Sequences flagged as poor quality 0 Sequence length 77 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75834222154503 38.0 33.0 41.0 2.0 41.0 2 32.53485448727716 38.0 33.0 40.0 2.0 41.0 3 32.33266036873381 38.0 32.0 40.0 2.0 41.0 4 32.13039006551882 38.0 32.0 40.0 2.0 41.0 5 32.00396160292549 38.0 32.0 40.0 2.0 41.0 6 31.723144903245466 37.0 32.0 40.0 2.0 41.0 7 31.40175986591498 37.0 31.0 40.0 2.0 41.0 8 31.057557519427093 36.0 31.0 40.0 2.0 41.0 9 30.831403321651685 35.0 31.0 40.0 2.0 41.0 10-11 30.754628218802377 35.0 30.5 40.0 2.0 41.0 12-13 30.53018817613896 35.0 30.0 40.0 2.0 41.0 14-15 30.17968154807253 35.0 30.0 40.0 2.0 41.0 16-17 29.653912082888922 35.0 27.5 40.0 2.0 41.0 18-19 29.127095078470212 35.0 25.0 40.0 2.0 41.0 20-21 28.446880237696178 35.0 21.0 40.0 2.0 41.0 22-23 27.627914063690383 35.0 5.5 40.0 2.0 41.0 24-25 26.939014170348926 35.0 2.0 40.0 2.0 41.0 26-27 25.92442480572909 34.0 2.0 39.0 2.0 40.5 28-29 25.784740210269696 34.5 2.0 39.0 2.0 40.0 30-31 25.87094316623495 35.0 2.0 39.0 2.0 41.0 32-33 25.573194423282036 35.0 2.0 39.0 2.0 41.0 34-35 25.26161816242572 35.0 2.0 39.0 2.0 41.0 36-37 24.863077098887704 34.0 2.0 39.0 2.0 41.0 38-39 24.532188023769617 34.0 2.0 38.0 2.0 40.5 40-41 24.117172024988573 33.0 2.0 37.5 2.0 40.0 42-43 23.737962821880238 33.0 2.0 37.0 2.0 40.0 44-45 23.314795063233277 33.0 2.0 36.5 2.0 39.5 46-47 22.84799253390218 32.5 2.0 36.0 2.0 39.0 48-49 22.399740972116412 32.0 2.0 35.5 2.0 39.0 50-51 21.984515465488343 31.5 2.0 35.0 2.0 38.0 52-53 21.555557671796436 31.0 2.0 35.0 2.0 37.0 54-55 21.11989562699985 30.5 2.0 35.0 2.0 36.5 56-57 20.75319975620905 30.0 2.0 35.0 2.0 36.0 58-59 20.374123876276094 29.0 2.0 35.0 2.0 36.0 60-61 20.077117933871705 29.0 2.0 35.0 2.0 35.0 62-63 19.762246685966787 29.0 2.0 35.0 2.0 35.0 64-65 19.41693966174006 28.5 2.0 34.5 2.0 35.0 66-67 19.02076032302301 27.0 2.0 34.0 2.0 35.0 68-69 18.68608106049063 25.5 2.0 34.0 2.0 35.0 70-71 18.29384427853116 25.0 2.0 34.0 2.0 35.0 72-73 17.922348773426787 23.5 2.0 34.0 2.0 35.0 74-75 17.456365229315864 19.0 2.0 34.0 2.0 35.0 76-77 16.33473640103611 2.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.058359484618812 1101 2 2.6918604694295922 1101 3 2.7972036381966596 1101 4 2.428967967981105 1101 5 3.4859393290575404 1101 6 2.457696895419513 1101 7 3.08200730043103 1101 8 2.8183540649746455 1101 9 3.666332830916968 1101 10-11 3.8600794302593933 1101 12-13 3.414530103185765 1101 14-15 3.249900018563057 1101 16-17 4.377334521355941 1101 18-19 3.615958423200052 1101 20-21 3.434269084246427 1101 22-23 2.759061912682121 1101 24-25 1.7276368762513599 1101 26-27 1.9307380625425914 1101 28-29 1.6681029780518024 1101 30-31 2.004535712146147 1101 32-33 1.39237507007984 1101 34-35 1.7725716873273143 1101 36-37 0.4402761603742995 1101 38-39 -1.5767574297744353 1101 40-41 -1.2264158218566443 1101 42-43 -0.2059223078648742 1101 44-45 -0.5880077337135639 1101 46-47 0.04843578506420343 1101 48-49 0.016672906584236813 1101 50-51 0.6354038868554319 1101 52-53 1.3107675979026077 1101 54-55 1.0175877812638063 1101 56-57 1.2188634963322578 1101 58-59 1.3050101830713388 1101 60-61 1.2683895695409895 1101 62-63 2.08529003422478 1101 64-65 2.804728676870443 1101 66-67 2.2406156681596947 1101 68-69 3.362528337010712 1101 70-71 1.706787122319131 1101 72-73 1.1355566897956813 1101 74-75 -0.38144689886749106 1101 76-77 -0.21612863077656996 1102 1 4.629788056047381 1102 2 6.025193802762928 1102 3 6.178156019149046 1102 4 6.428967967981105 1102 5 6.295463138581351 1102 6 6.552934990657608 1102 7 4.7486739670976945 1102 8 6.056449303069879 1102 9 7.428237592821727 1102 10-11 7.360079430259393 1102 12-13 7.485958674614341 1102 14-15 7.487995256658291 1102 16-17 8.591620235641656 1102 18-19 8.996910804152431 1102 20-21 9.886650036627376 1102 22-23 10.02096667458688 1102 24-25 10.156208304822787 1102 26-27 10.454547586352117 1102 28-29 10.191912501861328 1102 30-31 9.314059521669957 1102 32-33 10.416184593889358 1102 34-35 10.27257168732731 1102 36-37 10.654561874660015 1102 38-39 10.994671141654134 1102 40-41 8.86882227338145 1102 42-43 7.0797919778494105 1102 44-45 7.840563694857863 1102 46-47 7.167483404111824 1102 48-49 6.8023871922985215 1102 50-51 4.944927696379246 1102 52-53 4.739339026474035 1102 54-55 3.327111590787613 1102 56-57 4.409339686808448 1102 58-59 5.495486373547529 1102 60-61 5.792199093350511 1102 62-63 5.347194796129546 1102 64-65 4.542823914965677 1102 66-67 4.621568049112074 1102 68-69 4.2672902417726135 1102 70-71 5.444882360414372 1102 72-73 6.849842404081393 1102 74-75 5.761410243989655 1102 76-77 6.855299940652003 1103 1 -3.9354293352569663 1103 2 -3.390955265560052 1103 3 -4.347723898035223 1103 4 -3.235628305310822 1103 5 -3.4478080829507434 1103 6 -5.312489439984212 1103 7 -3.791698703709759 1103 8 -4.061563119290366 1103 9 -3.7891537115260974 1103 10-11 -3.046752867877256 1103 12-13 -2.8101075779736497 1103 14-15 -3.170389836509411 1103 16-17 -3.5118994330954045 1103 18-19 -1.85712646292831 1103 20-21 -2.6081739799357706 1103 22-23 -2.5100892260342356 1103 24-25 -2.0622181962124095 1103 26-27 -2.294934815304199 1103 28-29 -3.1114001275382606 1103 30-31 -3.547224122222385 1103 32-33 -4.171807124537143 1103 34-35 -2.858898291968753 1103 36-37 -3.4593097609507524 1103 38-39 -3.225825752755803 1103 40-41 -3.691219134486044 1103 42-43 -3.898468891715808 1103 44-45 -4.3229973817466885 1103 46-47 -4.594421357792939 1103 48-49 -3.0350869277842953 1103 50-51 -3.2351965272232412 1103 52-53 -3.8331247416419068 1103 54-55 -4.374751763249652 1103 56-57 -4.6827928183675365 1103 58-59 -4.380290023967998 1103 60-61 -4.081506910790274 1103 62-63 -4.116573319812485 1103 64-65 -4.659039439071588 1103 66-67 -4.268701102026641 1103 68-69 -3.506001683693224 1103 70-71 -2.938140413912752 1103 72-73 -2.4617517988171578 1103 74-75 -2.2520473129461642 1103 76-77 -1.2803108253935456 1104 1 -1.47021194395262 1104 2 -0.6605204829513553 1104 3 -2.7694630284700033 1104 4 -2.4638891748760408 1104 5 -3.402155909037699 1104 6 -1.7994459617233396 1104 7 -1.91561174718802 1104 8 -1.8506935540729756 1104 9 -2.2717624071782723 1104 10-11 -1.8934919983120402 1104 12-13 -1.4188032301475637 1104 14-15 -2.2084333147702786 1104 16-17 -0.49885595483453216 1104 18-19 -1.4245177672761393 1104 20-21 -1.4288261538488136 1104 22-23 -0.7861761825559732 1104 24-25 -0.18069645708197157 1104 26-27 -0.014500032695504927 1104 28-29 -1.0961827362339136 1104 30-31 -0.8407023830919478 1104 32-33 -0.2457201680153993 1104 34-35 -1.3643330745774485 1104 36-37 -0.32996193486379255 1104 38-39 -0.3160431440601492 1104 40-41 -0.6216539170947364 1104 42-43 -0.34520802215058666 1104 44-45 0.4596113139054836 1104 46-47 -1.0694213577929403 1104 48-49 -1.778565188653861 1104 50-51 -1.551500875049328 1104 52-53 -1.5439943068593003 1104 54-55 -1.1943169806409593 1104 56-57 -1.000184122715364 1104 58-59 -0.5009421978810416 1104 60-61 -0.30541995426853674 1104 62-63 0.03886146279620917 1104 64-65 -0.30360465646289114 1104 66-67 0.10847281101683848 1104 68-69 -0.12556690108452884 1104 70-71 0.4151204556524668 1104 72-73 -0.5834909292519406 1104 74-75 -0.39335166077225026 1104 76-77 -0.3292238688718072 1105 1 0.3497880560473803 1105 2 1.8194795170486415 1105 3 2.1705369715299945 1105 4 2.5261108251239612 1105 5 2.7678440909623028 1105 6 1.3205540382766578 1105 7 1.5543882528119823 1105 8 2.679306445927022 1105 9 0.8682375928217283 1105 10-11 1.7315080016879598 1105 12-13 2.9611967698524317 1105 14-15 2.296566685229717 1105 16-17 1.5611440451654666 1105 18-19 1.385482232723863 1105 20-21 0.11617384615118809 1105 22-23 0.5838238174440278 1105 24-25 -0.42569645708197257 1105 26-27 0.2754999673044942 1105 28-29 0.013817263766089383 1105 30-31 -0.21070238309194878 1105 32-33 -0.7857201680153985 1105 34-35 -1.129333074577449 1105 36-37 -2.014961934863795 1105 38-39 -1.4510431440601508 1105 40-41 -0.12165391709473994 1105 42-43 -0.7802080221505889 1105 44-45 -0.9803886860945141 1105 46-47 -0.614421357792942 1105 48-49 -1.798565188653857 1105 50-51 -0.9865008750493267 1105 52-53 -2.3739943068592986 1105 54-55 -3.184316980640954 1105 56-57 -4.02018412271536 1105 58-59 -3.235942197881041 1105 60-61 -3.1554199542685346 1105 62-63 -3.6061385372037904 1105 64-65 -2.6886046564628927 1105 66-67 -3.0165271889831633 1105 68-69 -2.915566901084528 1105 70-71 -2.1198795443475333 1105 72-73 -2.063490929251939 1105 74-75 -1.2233516607722486 1105 76-77 -2.5342238688718073 1106 1 0.44796987422920154 1106 2 1.78493406250319 1106 3 -1.1876448466518212 1106 4 1.1952017342148658 1106 5 0.4296622727804795 1106 6 0.600554038276659 1106 7 1.716206434630159 1106 8 2.2902155368361186 1106 9 2.3373285019126335 1106 10-11 2.4315080016879627 1106 12-13 0.967560406216073 1106 14-15 1.4620212306842681 1106 16-17 -0.0761286821072602 1106 18-19 0.15275495999658872 1106 20-21 -0.6220079720306337 1106 22-23 -0.5179943643741538 1106 24-25 -0.01478736617287879 1106 26-27 1.1991363309408634 1106 28-29 1.3174536274024504 1106 30-31 1.841115798726232 1106 32-33 1.7679161956209626 1106 34-35 0.8288487436043717 1106 36-37 0.9814017014998413 1106 38-39 1.1635023104853026 1106 40-41 1.0647097192688975 1106 42-43 2.170701068758504 1106 44-45 2.300520404814577 1106 46-47 2.405578642207061 1106 48-49 3.080525720437052 1106 50-51 3.078044579496126 1106 52-53 2.178732965867976 1106 54-55 0.9829557466317702 1106 56-57 1.4179976954664575 1106 58-59 0.42405780211895916 1106 60-61 1.8809436820951007 1106 62-63 2.286588735523484 1106 64-65 2.3123044344461974 1106 66-67 1.8380182655622903 1106 68-69 0.6926149170972913 1106 70-71 -0.6416977261657131 1106 72-73 1.4255999798389674 1106 74-75 1.9475574301368432 1106 76-77 2.6366852220372863 1107 1 -2.6502119439526197 1107 2 -1.580520482951357 1107 3 -2.309463028470006 1107 4 -2.91388917487604 1107 5 -2.8321559090376986 1107 6 -2.9994459617233424 1107 7 -3.445611747188021 1107 8 -5.800693554072975 1107 9 -6.571762407178273 1107 10-11 -4.2284919983120375 1107 12-13 -3.698803230147565 1107 14-15 -4.18343331477028 1107 16-17 -2.9388559548345334 1107 18-19 -4.7145177672761385 1107 20-21 -5.043826153848816 1107 22-23 -4.036176182555977 1107 24-25 -3.325696457081971 1107 26-27 -2.084500032695505 1107 28-29 -1.6661827362339103 1107 30-31 -1.3507023830919458 1107 32-33 -1.8857201680153999 1107 34-35 -2.049333074577447 1107 36-37 -1.174961934863795 1107 38-39 -0.6110431440601509 1107 40-41 -0.3616539170947384 1107 42-43 -2.520208022150591 1107 44-45 -0.7003886860945165 1107 46-47 -0.5544213577929398 1107 48-49 -2.418565188653858 1107 50-51 -1.6465008750493269 1107 52-53 -0.8539943068592954 1107 54-55 -2.164316980640958 1107 56-57 -0.5601841227153628 1107 58-59 -1.5159421978810386 1107 60-61 -2.2754199542685356 1107 62-63 -2.926138537203787 1107 64-65 -4.008604656462893 1107 66-67 -3.576527188983164 1107 68-69 -2.5155669010845294 1107 70-71 -2.259879544347534 1107 72-73 -4.003490929251939 1107 74-75 -4.3433516607722495 1107 76-77 -5.254223868871808 1108 1 -4.233848307588982 1108 2 -4.078702301133173 1108 3 -5.824008483015458 1108 4 -5.122980083966951 1108 5 -4.570337727219517 1108 6 -4.763082325359704 1108 7 -5.329248110824384 1108 8 -5.255239008618428 1108 9 -4.344489679905546 1108 10-11 -3.4094010892211273 1108 12-13 -2.623348684693017 1108 14-15 -1.8788878602248253 1108 16-17 -2.4852195911981667 1108 18-19 -2.415426858185228 1108 20-21 -2.5310988811215402 1108 22-23 -1.5407216371014272 1108 24-25 -4.469332820718339 1108 26-27 -5.20995457815005 1108 28-29 -4.318910008961186 1108 30-31 -4.545247837637401 1108 32-33 -3.9366292589244942 1108 34-35 -4.262060347304722 1108 36-37 -3.5867801166819753 1108 38-39 -4.018315871332877 1108 40-41 -3.503472098912919 1108 42-43 -2.8520262039687694 1108 44-45 -5.222206867912696 1108 46-47 -4.253512266883845 1108 48-49 -3.374019734108405 1108 50-51 -3.240137238685694 1108 52-53 -3.753085215950204 1108 54-55 -3.0624987988227765 1108 56-57 -2.4456386681699094 1108 58-59 -2.3032149251537675 1108 60-61 -2.323601772450356 1108 62-63 -1.8952294462946995 1108 64-65 -2.7104228382810724 1108 66-67 -1.7074362798922564 1108 68-69 -1.3982941738118022 1108 70-71 -1.9144249988929865 1108 72-73 -2.3471272928883025 1108 74-75 -4.938806206226797 1108 76-77 -4.204223868871807 1109 1 0.9497880560473817 1109 2 0.019479517048644368 1109 3 0.8505369715299942 1109 4 -0.23388917487604033 1109 5 0.6478440909622982 1109 6 0.6405540382766581 1109 7 1.1143882528119775 1109 8 2.239306445927024 1109 9 1.9882375928217293 1109 10-11 2.451508001687966 1109 12-13 1.3011967698524352 1109 14-15 1.4365666852297245 1109 16-17 1.8211440451654681 1109 18-19 2.1654822327238605 1109 20-21 3.3161738461511874 1109 22-23 2.603823817444024 1109 24-25 3.3143035429180294 1109 26-27 2.995499967304493 1109 28-29 3.15381726376609 1109 30-31 3.6692976169080502 1109 32-33 4.454279831984596 1109 34-35 5.2506669254225535 1109 36-37 4.645038065136202 1109 38-39 4.16895685593985 1109 40-41 5.0983460829052625 1109 42-43 3.2997919778494094 1109 44-45 4.759611313905481 1109 46-47 5.065578642207061 1109 48-49 3.8214348113461405 1109 50-51 3.913499124950672 1109 52-53 4.226005693140703 1109 54-55 4.195683019359041 1109 56-57 3.659815877284636 1109 58-59 1.2240578021189599 1109 60-61 0.2845800457314631 1109 62-63 1.4138614627962092 1109 64-65 1.5313953435371097 1109 66-67 2.223472811016837 1109 68-69 2.2044330989154695 1109 70-71 2.7601204556524657 1109 72-73 1.6565090707480614 1109 74-75 1.7366483392277523 1109 76-77 1.4857761311281905 1110 1 3.000158426417755 1110 2 2.9617017392708647 1110 3 2.574981415974438 1110 4 2.5083330473461807 1110 5 2.1737700168882235 1110 6 3.9709244086470328 1110 7 2.849203067626796 1110 8 3.4585657051862846 1110 9 3.2060153705995056 1110 10-11 3.135211705391672 1110 12-13 1.4886041772598446 1110 14-15 2.805455574118607 1110 16-17 2.141884785906207 1110 18-19 1.9810377882794192 1110 20-21 2.3813590313363733 1110 22-23 3.200860854481064 1110 24-25 3.560970209584692 1110 26-27 3.3725370043415346 1110 28-29 2.0278913378401633 1110 30-31 0.5944828020932356 1110 32-33 0.8024279801327445 1110 34-35 0.3810372957929218 1110 36-37 1.1228158429139832 1110 38-39 1.7089568559398494 1110 40-41 2.1227905273497036 1110 42-43 2.6353475334049676 1110 44-45 2.348500202794373 1110 46-47 2.7203934570218777 1110 48-49 2.7362496261609586 1110 50-51 2.6327583842099322 1110 52-53 2.8319316190666264 1110 54-55 1.5519793156553412 1110 56-57 1.0072232846920457 1110 58-59 2.1647985428596996 1110 60-61 2.189024490175907 1110 62-63 1.4953429442776915 1110 64-65 1.9899138620556265 1110 66-67 2.2168061443501728 1110 68-69 2.3281368026191736 1110 70-71 3.243824159356169 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20-21 -2.407274429710885 2315 22-23 -0.7327279066939028 2315 24-25 -0.6401792157026627 2315 26-27 -0.9920862395920551 2315 28-29 -0.7703206672683969 2315 30-31 -1.0907023830919478 2315 32-33 -1.225030512842988 2315 34-35 -0.33416066078434525 2315 36-37 -1.982548141760347 2315 38-39 -3.0324224544049763 2315 40-41 -3.0285504688188745 2315 42-43 -2.95469078077128 2315 44-45 -2.126595582646239 2315 46-47 -2.2323523922757005 2315 48-49 -2.802703119688342 2315 50-51 -1.9023629440148433 2315 52-53 -1.4043391344455038 2315 54-55 -2.227075601330611 2315 56-57 -1.6760461916808786 2315 58-59 -1.00697668063966 2315 60-61 -1.0830061611650876 2315 62-63 -0.4344143992727574 2315 64-65 -0.42515638060082495 2315 66-67 -0.7199754648452341 2315 68-69 -0.892808280394874 2315 70-71 -1.8391898891751204 2315 72-73 -2.121421963734697 2315 74-75 -1.5054206262894922 2315 76-77 -0.6266376619752556 2316 1 -1.9773548010954762 2316 2 -1.0105204829513568 2316 3 -0.2980344570414317 2316 4 0.42896796798110515 2316 5 -1.359298766180558 2316 6 -0.29230310458048336 2316 7 -2.179897461473736 2316 8 -1.9078364112158326 2316 9 -0.3574766928925577 2316 10-11 -0.4970634268834644 2316 12-13 -0.383088944433279 2316 14-15 -0.5120047433417092 2316 16-17 -1.7774273834059606 2316 18-19 -2.270946338704711 2316 20-21 -2.3395404395630983 2316 22-23 -2.336176182555974 2316 24-25 -2.6771250285105417 2316 26-27 -1.3966428898383612 2316 28-29 -1.2783255933767705 2316 30-31 -1.7157023830919478 2316 32-33 -1.5421487394439701 2316 34-35 -1.042904503148879 2316 36-37 -0.38710479200665304 2316 38-39 -0.39818600120300474 2316 40-41 -0.9287967742375933 2316 42-43 -2.1702080221505895 2316 44-45 -1.093960114665947 2316 46-47 -1.3622785006500848 2316 48-49 -1.4357080457967157 2316 50-51 -1.5729294464778967 2316 52-53 -1.5404228782878668 2316 54-55 -1.9586026949266717 2316 56-57 -2.0608984084296473 2316 58-59 -2.218799340738183 2316 60-61 -3.951848525697107 2316 62-63 -3.16470996577522 2316 64-65 -3.117890370748608 2316 66-67 -2.3308129032688782 2316 68-69 -2.6969954725130982 2316 70-71 -2.942022401490391 2316 72-73 -4.3763480721090815 2316 74-75 -3.7683516607722503 2316 76-77 -3.007795297443236 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 3341.0 3 159.0 4 258.0 5 332.0 6 329.0 7 329.0 8 494.0 9 509.0 10 618.0 11 679.0 12 472.0 13 311.0 14 283.0 15 249.0 16 296.0 17 260.0 18 233.0 19 238.0 20 251.0 21 297.0 22 306.0 23 264.0 24 309.0 25 284.0 26 348.0 27 395.0 28 453.0 29 500.0 30 611.0 31 686.0 32 772.0 33 938.0 34 1463.0 35 1734.0 36 2084.0 37 2689.0 38 1979.0 39 495.0 40 4.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.267362566193 29.094441693016876 33.902243895005526 18.7359518457846 2 18.704049369547825 29.9455258847282 33.922517237438576 17.427907508285397 3 18.43879766848261 29.646843689283404 33.13650043811192 18.77785820412206 4 18.70237732398659 29.346997866504115 34.55882352941176 17.391801280097532 5 18.892148272315133 28.86586155663073 34.10796601775306 18.13402415330108 6 18.514285714285712 30.438095238095237 33.58857142857143 17.459047619047617 7 18.58117118146836 29.892940145540443 33.80576827827942 17.720120394711778 8 18.273260687342834 29.68452335593995 34.02042215956717 18.021793797150043 9 18.0592067664876 29.889130186307007 34.14866460928868 17.902998437916715 10-11 17.387411415072773 30.534176636439835 34.23378800579136 17.844623942696032 12-13 17.662634536622534 30.698161729688543 33.315553862272594 18.323649871416325 14-15 18.111856141420297 29.927994513867723 33.425403840292596 18.534745504419384 16-17 17.584761904761905 30.060952380952383 33.645714285714284 18.708571428571428 18-19 18.45318601771597 29.80855319554243 32.90027621678255 18.837984569959044 20-21 18.76774181257025 29.570005143934917 32.48490159843015 19.17735144506468 22-23 18.684751962203762 29.84835784500495 32.34016612055171 19.12672407223958 24-25 19.059773705664977 29.648748523753287 31.843117833060308 19.448359937521428 26-27 19.437564302861716 29.607895438783675 31.03113211142019 19.92340814693442 28-29 19.008799664774674 29.153175117138396 31.196906784503447 20.641118433583483 30-31 18.615724516227335 29.647264970287978 30.87764741733963 20.859363096145056 32-33 18.691428571428574 29.350476190476186 30.89904761904762 21.059047619047618 34-35 18.54285714285714 28.514285714285712 31.17142857142857 21.771428571428572 36-37 18.18510828777691 28.47863768833692 31.503457208708735 21.83279681517743 38-39 18.520352768624164 27.800529533895883 31.701556220118476 21.977561477361473 40-41 17.917753947543858 28.029942286813586 31.989866859678862 22.062436905963693 42-43 18.118440351244786 28.076725270957542 31.663460256385832 22.141374121411836 44-45 18.456439754676012 28.322730562645233 31.259761532893986 21.961068149784772 46-47 18.460307481282506 27.555199939037166 31.742584443047377 22.241908136632947 48-49 19.06158916426952 27.424608995485112 30.99841883679729 22.51538300344808 50-51 18.695809592773976 27.775978047525584 30.36377841721135 23.16443394248909 52-53 19.0625 27.614329268292682 30.38109756097561 22.942073170731707 54-55 19.668883004057992 27.14664024843301 30.303492160262152 22.880984587246854 56-57 19.359570252971654 26.54487960987504 30.611856141420297 23.483693995733006 58-59 19.96190113344128 26.76635870082865 29.920944851890656 23.350795313839413 60-61 19.695988418606422 26.993409272734198 29.5477922968494 23.76281001180997 62-63 20.22596071408158 26.263646236210867 29.302493950883076 24.20789909882447 64-65 20.64734906936427 26.49025547236669 28.980206130574764 23.882189327694274 66-67 20.71934771012726 26.407833574639945 29.579745485026287 23.293073230206506 68-69 20.92996665078609 25.715102429728443 29.913292043830396 23.441638875655073 70-71 20.82221544560494 26.065411298315162 29.53038042235267 23.581992833727224 72-73 21.151169663114146 25.458046557739607 28.952736840098375 24.438046939047872 74-75 22.049150635831538 25.265485882061352 28.876475186364413 23.808888295742694 76-77 22.09087269052567 25.45045474478998 29.265734932407955 23.192937632276394 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1088.0 1 618.5 2 134.5 3 113.5 4 107.0 5 107.5 6 102.5 7 104.0 8 93.5 9 76.0 10 85.5 11 100.0 12 111.5 13 118.0 14 127.0 15 134.0 16 143.0 17 154.0 18 168.0 19 200.0 20 239.0 21 265.0 22 270.0 23 296.5 24 342.0 25 388.0 26 415.0 27 428.0 28 473.0 29 515.5 30 526.0 31 539.5 32 602.5 33 631.5 34 664.5 35 718.0 36 709.0 37 736.5 38 729.5 39 686.0 40 681.5 41 681.5 42 734.5 43 783.0 44 831.5 45 889.5 46 987.0 47 1139.5 48 1204.0 49 1173.0 50 1197.0 51 1162.0 52 1072.0 53 987.5 54 841.5 55 748.0 56 666.5 57 617.0 58 613.5 59 563.0 60 443.0 61 370.0 62 341.0 63 256.5 64 189.5 65 178.0 66 145.5 67 111.0 68 92.0 69 66.0 70 57.0 71 55.5 72 49.5 73 38.0 74 31.0 75 28.5 76 28.5 77 22.5 78 14.0 79 11.0 80 9.0 81 7.0 82 3.0 83 2.0 84 3.0 85 2.5 86 1.0 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011427700746609783 2 0.0038092335822032604 3 0.011427700746609783 4 0.015236934328813042 5 0.011427700746609783 6 0.007618467164406521 7 0.019046167911016303 8 0.022855401493219567 9 0.019046167911016303 10-11 0.022855401493219567 12-13 0.017141551119914673 14-15 0.015236934328813042 16-17 0.007618467164406521 18-19 0.017141551119914673 20-21 0.028569251866524453 22-23 0.022855401493219567 24-25 0.011427700746609783 26-27 0.03428310223982935 28-29 0.0038092335822032604 30-31 0.0 32-33 0.007618467164406521 34-35 0.007618467164406521 36-37 0.009523083955508152 38-39 0.009523083955508152 40-41 0.005713850373304892 42-43 0.009523083955508152 44-45 0.0038092335822032604 46-47 0.024760018284321193 48-49 0.020950784702117933 50-51 0.05142465335974402 52-53 0.045710802986439134 54-55 0.028569251866524453 56-57 0.015236934328813042 58-59 0.017141551119914673 60-61 0.011427700746609783 62-63 0.03237848544872771 64-65 0.024760018284321193 66-67 0.022855401493219567 68-69 0.05523388694194728 70-71 0.0685662044796587 72-73 0.10094468992838641 74-75 0.10094468992838641 76-77 0.10856315709279293 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 26252.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.80740515008381 #Duplication Level Percentage of deduplicated Percentage of total 1 89.57700969352786 69.69754685357306 2 6.232253010868501 9.698308700289502 3 2.012141388426515 4.69678500685662 4 0.9057084108489181 2.818832850830413 5 0.40634485459708214 1.5808319366143533 6 0.3035347106628806 1.417034892579613 7 0.1615587976108881 0.8799329574889532 8 0.14687163419171645 0.9142160597287825 9 0.06364437481641046 0.4456803291177815 >10 0.1811416821697836 1.809385951546549 >50 0.0 0.0 >100 0.0 0.0 >500 0.009791442279447763 6.041444461374371 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 863 3.287368581441414 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 723 2.7540758799329574 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.019046167911016303 0.0 3 0.0 0.0 0.0 0.03428310223982935 0.0 4 0.0 0.0 0.0 0.07618467164406521 0.0 5 0.0 0.0 0.0 0.13332317537711413 0.0 6 0.0 0.0 0.0 0.20950784702117933 0.0 7 0.0 0.0 0.0 0.3085479201584641 0.0 8 0.0 0.0 0.0 0.36949565747371627 0.0 9 0.0 0.0 0.0 0.4799634313576108 0.0 10 0.0 0.0 0.0 0.5904312052415054 0.0 11 0.0 0.0 0.0 0.7466097821118391 0.0 12 0.0 0.0 0.0 0.8837421910711565 0.0 13 0.0 0.0 0.0 1.0856315709279294 0.0 14 0.0 0.0 0.0 1.257047082127076 0.0 15 0.0 0.0 0.0 1.4017979582508 0.0 16 0.0 0.0 0.0 1.5694042358677434 0.0 17 0.0 0.0 0.0 1.7141551119914673 0.0 18 0.0 0.0 0.0 1.8055767179643456 0.0 19 0.0 0.0 0.0 1.9084260246838336 0.0 20 0.0 0.0 0.0 2.0074660978211183 0.0 21 0.0 0.0 0.0 2.1407892731982323 0.0 22 0.0 0.0 0.0 2.27792168215755 0.0 23 0.0 0.0 0.0 2.3921986896236476 0.0 24 0.0 0.0 0.0 2.525521865000762 0.0 25 0.0 0.0 0.0 2.6207527045558434 0.0 26 0.0 0.0 0.0 2.773122047843974 0.0 27 0.0 0.0 0.0 2.8721621209812587 0.0 28 0.0 0.0 0.0 2.9597744933719334 0.0 29 0.0 0.0 0.0 3.058814566509218 0.0 30 0.0 0.0 0.0 3.1769008075575194 0.0 31 0.0 0.0 0.0 3.3102239829346334 0.0 32 0.0 0.0 0.0 3.474021026969374 0.0 33 0.0 0.0 0.0 3.637818071004114 0.0 34 0.0 0.0 0.0 3.797805881456651 0.0 35 0.0 0.0 0.0 3.9387475239981717 0.0 36 0.0 0.0 0.0 4.102544568032911 0.0 37 0.0 0.0 0.0 4.23967697699223 0.0 38 0.0 0.0 0.0 4.418710955355783 0.0 39 0.0 0.0 0.0 4.586317232972726 0.0 40 0.0 0.0 0.0 4.765351211336279 0.0 41 0.0 0.0 0.0 4.948194423282036 0.0 42 0.0 0.0 0.0 5.138656102392199 0.0 43 0.0 0.0 0.0 5.355782416577784 0.0 44 0.0 0.0 0.0 5.557671796434557 0.0 45 0.0 0.0 0.0 5.751942709126924 0.0 46 0.0 0.0 0.0 5.999542891970136 0.0 47 0.0 0.0 0.0 6.1938138046625015 0.0 48 0.0 0.0 0.0 6.452841688252324 0.0 49 0.0 0.0 0.0 6.68139570318452 0.0 50 0.0 0.0 0.0 6.883285083041292 0.0 51 0.0 0.0 0.0 7.16135913454213 0.0 52 0.0 0.0 0.0 7.450860886789578 0.0 53 0.0 0.0 0.0 7.717507237543806 0.0 54 0.0 0.0 0.0 7.968916653969221 0.0 55 0.0 0.0 0.0 8.254609172634465 0.0 56 0.0 0.0 0.0 8.532683224135305 0.0 57 0.0 0.0 0.0 8.871705012951395 0.0 58 0.0 0.0 0.0 9.210726801767484 0.0 59 0.0 0.0 0.0 9.523083955508152 0.0 60 0.0 0.0 0.0 9.785921072680177 0.0 61 0.0 0.0 0.0 10.1630351973183 0.0 62 0.0 0.0 0.0 10.437300015236934 0.0 63 0.0 0.0 0.0 10.772512570470822 0.0 64 0.0 0.0 0.0 11.176291330184366 0.0 65 0.0 0.0 0.0 11.538168520493677 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACATT 15 0.0020822992 70.98286 22 AGAGGCC 20 0.0064589796 53.33874 4 AAGTAGA 20 0.0065080104 53.23714 48 CCTACAT 20 0.0065080104 53.23714 21 CCCTACA 20 0.0065080104 53.23714 20 >>END_MODULE