FastQCFastQC Report
Thu 26 May 2016
SRR936121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936121_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26252
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT6212.365534054548225No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4391.6722535425872314No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3861.4703641627304587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3781.4398902940728324No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT3621.3789425567575804RNA PCR Primer, Index 26 (100% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.963736096297425No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.8685052567423435No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT2110.803748285844888RNA PCR Primer, Index 26 (100% over 24bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG2060.7847021179338717RNA PCR Primer, Index 26 (100% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1330.5066280664330337No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.43425262837117173No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT1040.3961602925491391RNA PCR Primer, Index 26 (100% over 27bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.3275940880694804No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.2742648179186348No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG610.2323632485143989RNA PCR Primer, Index 26 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC580.22093554776778912RNA PCR Primer, Index 26 (95% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA480.18284321194575653No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT470.17903397836355325RNA PCR Primer, Index 26 (100% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.17903397836355325No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.17522474478135No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.14856010970592717No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA380.14475087612372392No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA370.14094164254152064RNA PCR Primer, Index 26 (100% over 29bp)
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.1371324089593174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG310.11808624104830108No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG310.11808624104830108No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGAG300.11427700746609781No Hit
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC280.1066585403016913No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT280.1066585403016913No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT270.10284930671948804No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG152.6720384E-4119.000015
GTACCCA251.6943506E-595.21
ATGGGGG250.0020344671.45
AGTATAC250.0020344671.43
ATAGTAT250.0020344671.41
ATGCTTA150.00421409859.50000430-31
CTGGTAT150.00421409859.500004100-101
CCGTACA150.00421409859.500004100-101
GTATACT300.004190645659.5000044
GCCAGTT150.00421409859.50000416-17
CGGTATC452.3646862E-1159.500004100-101
CCTATCA150.00421409859.500004100-101
GTTATCA150.00421409859.500004100-101
TAGTATA300.004190645659.5000042
CTTACTT150.00421409859.50000434-35
ACGGTAT150.00421409859.500004100-101
TACGCAG257.3537667E-659.5100-101
AAGTATC201.7563705E-459.499996100-101
CGTATCA405.5479177E-1059.499996100-101
CCCCGTA201.7563705E-459.49999698-99
Produced by FastQC (version 0.11.5)