FastQCFastQC Report
Thu 26 May 2016
SRR936120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26139
Sequences flagged as poor quality0
Sequence length77
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8713.332185623015418No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7252.773633268296415No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA270.10329392861241822No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAACG200.006483891553.28598868
CCAGCGC200.006533396453.18390737
ATTGCTG200.006533396453.18390725
CGCATCT200.006533396453.18390710
TCAACGC900.004771688519.6977424
ATCAACG950.006522164718.661023
CAACGCA950.006522164718.661025
TATCAAC1000.00876767217.7279682
Produced by FastQC (version 0.11.5)