FastQCFastQC Report
Thu 26 May 2016
SRR936120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26139
Sequences flagged as poor quality0
Sequence length125
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT6202.3719346570259No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4681.7904280959485825No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4301.6450514556792533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3891.4881977122307664No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT3571.365775278319752RNA PCR Primer, Index 26 (100% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.9755537702283943No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG1910.730708902406366RNA PCR Primer, Index 26 (100% over 29bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT1840.7039289949883316RNA PCR Primer, Index 26 (100% over 24bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.6848004896897356No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.43612992080798807No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT1130.43230421974826894RNA PCR Primer, Index 26 (100% over 27bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.38639580703163856No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.3098817858372547No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.29075328053865873No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.2218906614637132No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG550.21041355828455566RNA PCR Primer, Index 26 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC440.1683308466276445RNA PCR Primer, Index 26 (95% over 22bp)
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.16067944450820612No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA410.15685374344848693RNA PCR Primer, Index 26 (100% over 29bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA400.15302804238876774No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.15302804238876774No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA390.14920234132904855No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT360.137725238149891RNA PCR Primer, Index 26 (100% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC340.13007383603045258No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG340.13007383603045258No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT320.12242243391101419No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGAG290.1109453307318566No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA290.1109453307318566No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.10711962967213742No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG270.10329392861241822No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGATC150.004189515559.58733102-103
TGATCTC150.004189515559.58733104-105
CGTACAT405.529728E-1059.53021100-101
CGTATCA750.059.5302100-101
CTGGTAT257.330993E-659.5302100-101
ACGTATC150.00420548259.530197100-101
CCCCGTA150.00422149459.4731898-99
CCTCCGT150.00422149459.4731896-97
ACCCGTA150.00422149459.4731898-99
AAAGGTA150.00422149459.4731898-99
CGGTATC600.054.569347100-101
AGGTATC451.5734258E-952.915737100-101
CCGTACA403.7374775E-852.088932100-101
GGGTATC358.941315E-751.025887100-101
ATGGGGG350.007725420450.977015
TCAAGGT350.007725420450.977013
CCGTATC302.1579574E-549.608498100-101
CCCGTAC302.1702517E-549.5609898-99
CCCGGTA302.1702517E-549.5609898-99
GCGTATC255.272417E-447.62416100-101
Produced by FastQC (version 0.11.5)