FastQCFastQC Report
Thu 26 May 2016
SRR936119_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936119_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1841690
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47690.2589469454685642No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20780.11283114965059265No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCA950.006648442218.6827440
CTAAGCG1452.1934023E-417.13425311
TAAGCGT1250.001604625417.03819512
AACGTAC2803.2359821E-916.48487351
CTCGAAC6300.015.77910623
TCGAACT6350.015.65443524
CCTCGAA6700.015.36696522
GCCTCGA6300.015.21515321
CGAACTC6800.015.13933625
AAGGTCG4251.8189894E-1215.03533654
CCCGATA1450.0043180614.68809838
CGATACT2001.5576255E-414.19772531
AATCCGC5050.014.05753535
CGGCTAG3102.0128391E-713.7389873
CGTGCGC3404.1931344E-813.57504146
AGGTCGG4505.4569682E-1113.41005555
ATTCGAT1600.00828186513.31325961
TAAGGCG4851.4551915E-1113.17636722
AGTCGTG3253.5779158E-713.10487911
GGCCTCG8250.012.90982620
Produced by FastQC (version 0.11.5)