##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936119_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1841690 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.660579141983725 39.0 36.0 41.0 30.0 41.0 2 36.51732756327069 39.0 36.0 41.0 30.0 41.0 3 36.42798407983971 39.0 36.0 41.0 30.0 41.0 4 36.30234730057719 39.0 36.0 41.0 29.0 41.0 5 36.227701730475815 39.0 36.0 41.0 29.0 41.0 6 36.13778811852158 39.0 35.0 41.0 29.0 41.0 7 35.97083656858646 39.0 35.0 40.0 29.0 41.0 8 35.7811081126574 39.0 35.0 40.0 27.0 41.0 9 35.68804684827523 38.0 35.0 40.0 27.0 41.0 10-11 35.88689546014801 39.0 35.0 40.0 28.5 41.0 12-13 36.05103111815778 39.0 35.0 41.0 29.0 41.0 14-15 35.98927072417182 39.0 35.0 41.0 28.5 41.0 16-17 35.82887755268259 39.0 35.0 41.0 27.5 41.0 18-19 35.67387155275861 39.0 35.0 41.0 27.0 41.0 20-21 35.47175746189641 39.0 35.0 41.0 26.5 41.0 22-23 35.225976684458296 39.0 35.0 41.0 26.0 41.0 24-25 35.00792641541193 39.0 34.5 41.0 24.5 41.0 26-27 34.06535681900863 38.0 33.0 40.0 23.5 40.5 28-29 34.09636502342957 38.0 33.5 39.5 23.5 40.5 30-31 34.474934163730055 38.0 34.0 40.0 23.5 41.0 32-33 34.31812601469302 38.0 34.0 40.0 23.0 41.0 34-35 34.04652411643654 38.0 33.0 40.0 22.0 41.0 36-37 33.762097584284 38.0 33.0 40.0 21.5 41.0 38-39 33.49095070288702 37.0 33.0 40.0 20.5 41.0 40-41 33.10597983373967 36.5 32.5 40.0 19.5 41.0 42-43 32.751999522178 36.0 32.0 39.0 18.0 41.0 44-45 32.38592054037325 36.0 32.0 39.0 16.0 41.0 46-47 32.00071673300067 35.0 31.5 39.0 13.0 40.0 48-49 31.533985904250986 35.0 31.0 38.0 8.0 40.0 50-51 31.101523057626416 35.0 31.0 37.0 7.0 39.0 52-53 30.65236033208629 35.0 30.5 36.5 2.0 39.0 54-55 30.187029576095867 34.5 30.0 36.0 2.0 38.5 56-57 29.73589121947776 34.0 29.5 36.0 2.0 37.0 58-59 29.309404677225807 34.0 29.0 35.0 2.0 37.0 60-61 28.9574564666149 34.0 29.0 35.0 2.0 36.5 62-63 28.619457400539723 34.0 29.0 35.0 2.0 36.0 64-65 28.240833419305098 34.0 29.0 35.0 2.0 36.0 66-67 27.86488442680364 34.0 27.5 35.0 2.0 35.0 68-69 27.50686760529731 34.0 27.0 35.0 2.0 35.0 70-71 27.0845182956958 33.5 26.0 35.0 2.0 35.0 72-73 26.608434372777175 33.0 25.0 35.0 2.0 35.0 74-75 26.025844740428628 33.0 24.5 35.0 2.0 35.0 76-77 24.316829922516817 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1402980995610363 1101 2 1.045956303067662 1101 3 1.0813214704569134 1101 4 1.0806206315968794 1101 5 1.1632127881441932 1101 6 1.1503389519305287 1101 7 1.1712980026241269 1101 8 1.3224673563644203 1101 9 1.3955510062460306 1101 10-11 1.183796046007167 1101 12-13 1.3580699830966552 1101 14-15 1.298884209239553 1101 16-17 1.4036679568191701 1101 18-19 1.3709190650453493 1101 20-21 1.3080110487789227 1101 22-23 1.2831061375525437 1101 24-25 1.2365353789723414 1101 26-27 1.146023004118227 1101 28-29 1.3089875246585336 1101 30-31 1.3382093276968519 1101 32-33 1.4318214756783476 1101 34-35 1.5735911749266123 1101 36-37 1.5155880447934678 1101 38-39 1.427675042351538 1101 40-41 1.290389799043318 1101 42-43 1.2526626193751866 1101 44-45 1.2631894629291125 1101 46-47 1.3104031093843744 1101 48-49 1.514483241504557 1101 50-51 1.3036772196490567 1101 52-53 1.425306728102246 1101 54-55 1.3483867214062606 1101 56-57 1.2499891326488104 1101 58-59 1.244863944788726 1101 60-61 1.2412857540131554 1101 62-63 1.1950300317078977 1101 64-65 0.9991657133312515 1101 66-67 1.1140889909880514 1101 68-69 1.1056984592185337 1101 70-71 1.2006708100590409 1101 72-73 1.1415170969580934 1101 74-75 1.306274980122403 1101 76-77 1.399357612695276 1102 1 0.7201291597813224 1102 2 0.8753014248495958 1102 3 0.7247290007629417 1102 4 1.003478387778209 1102 5 0.9869343162387807 1102 6 0.9963766435949921 1102 7 0.7817276278025673 1102 8 0.829728541417218 1102 9 0.6985637693330489 1102 10-11 0.633782956322392 1102 12-13 0.9979797507998569 1102 14-15 0.8784827480551627 1102 16-17 0.8498352934346869 1102 18-19 0.9458634937183561 1102 20-21 0.9871053677820925 1102 22-23 1.0288722412707116 1102 24-25 1.0976100071129906 1102 26-27 0.9753421674535545 1102 28-29 0.9259253558358438 1102 30-31 1.0942244152615075 1102 32-33 1.0612035284133867 1102 34-35 0.8746128699284839 1102 36-37 0.9143235922902448 1102 38-39 0.9995083235838749 1102 40-41 0.9279202265770934 1102 42-43 1.1212467867847025 1102 44-45 0.8554003088467681 1102 46-47 0.9536261690273093 1102 48-49 1.0565696157502416 1102 50-51 1.0313725624828116 1102 52-53 0.921713600426088 1102 54-55 0.9176005928637174 1102 56-57 0.8787487349727705 1102 58-59 0.9149773776773031 1102 60-61 0.9481261544875501 1102 62-63 0.8572301886368336 1102 64-65 0.8759987754816692 1102 66-67 0.8582629766479393 1102 68-69 0.7928912612542653 1102 70-71 0.840483187561869 1102 72-73 0.7644513293005488 1102 74-75 0.8853893294730142 1102 76-77 1.0306926243066172 1103 1 0.5144734709637007 1103 2 0.44199593891253386 1103 3 0.5454045721947125 1103 4 0.3591664585297991 1103 5 0.4921650388520433 1103 6 0.43411424003394217 1103 7 0.5773138688415216 1103 8 0.49843201572666374 1103 9 0.5833843776866274 1103 10-11 0.5666323211136799 1103 12-13 0.6017918566284948 1103 14-15 0.6863874608802263 1103 16-17 0.7726772002451341 1103 18-19 0.7442419430344884 1103 20-21 0.9393742904079403 1103 22-23 0.7793777088562379 1103 24-25 0.6499785828733806 1103 26-27 0.5112284988631757 1103 28-29 0.6545076672977714 1103 30-31 0.7643816090121618 1103 32-33 0.8735248809646237 1103 34-35 0.8957048719642344 1103 36-37 0.8558803364281573 1103 38-39 0.7630039080110933 1103 40-41 0.7741988648500779 1103 42-43 0.8698077379066547 1103 44-45 0.8510567551989183 1103 46-47 0.6133053798257038 1103 48-49 0.725005819072507 1103 50-51 0.8240807057656667 1103 52-53 0.8228089313459783 1103 54-55 0.8399687738525863 1103 56-57 0.7797773815146272 1103 58-59 0.8735797157916423 1103 60-61 0.892644751960372 1103 62-63 0.8799082119198154 1103 64-65 0.687025384148928 1103 66-67 0.6254315366174694 1103 68-69 0.3745945616508486 1103 70-71 0.5092170699476988 1103 72-73 0.5509348859998795 1103 74-75 0.3359639354216881 1103 76-77 0.5567128686168417 1104 1 0.5841946023550975 1104 2 0.6144565522207017 1104 3 0.677257212974169 1104 4 0.4171183816965538 1104 5 0.6878893280299962 1104 6 0.39836820690382524 1104 7 0.5562450034197681 1104 8 0.4205340648969198 1104 9 0.5342964714278367 1104 10-11 0.41772119692297593 1104 12-13 0.6238243902037652 1104 14-15 0.603962345854228 1104 16-17 0.6454465333833141 1104 18-19 0.6247422477058748 1104 20-21 0.5464691371535579 1104 22-23 0.5266656836963719 1104 24-25 0.5719654070902749 1104 26-27 0.31920819020562874 1104 28-29 0.4373984226232821 1104 30-31 0.6705698118454535 1104 32-33 0.791423468472793 1104 34-35 0.816850519419468 1104 36-37 0.7569154232915096 1104 38-39 0.6630171952129231 1104 40-41 0.7411794762620545 1104 42-43 0.7693841737462463 1104 44-45 0.7687088028293871 1104 46-47 0.6167522668315399 1104 48-49 0.6514962780657498 1104 50-51 0.5883330463374143 1104 52-53 0.6250943300606515 1104 54-55 0.7347310211751612 1104 56-57 0.7801844750810965 1104 58-59 0.6031370616592326 1104 60-61 0.44021466461432013 1104 62-63 0.5877636471639143 1104 64-65 0.6661276260528197 1104 66-67 0.4921759541760906 1104 68-69 0.43866274990973864 1104 70-71 0.41256911263006657 1104 72-73 0.4850000880985661 1104 74-75 0.4709869925230947 1104 76-77 0.35506210679996997 1105 1 0.02055691578448915 1105 2 0.04436598991868124 1105 3 0.19067793056537852 1105 4 0.18559507602051895 1105 5 -0.1171740444020486 1105 6 -0.01971186956053117 1105 7 0.17612758089441627 1105 8 0.1901527371758931 1105 9 0.023549136868602716 1105 10-11 0.2195632555303959 1105 12-13 0.3126067028338042 1105 14-15 -0.019391427848304943 1105 16-17 0.23745603540508142 1105 18-19 0.4153561658995315 1105 20-21 0.4164517188299186 1105 22-23 0.42733768460366406 1105 24-25 0.278236413523679 1105 26-27 0.3659397864434908 1105 28-29 0.4746206526264629 1105 30-31 0.32952883898818186 1105 32-33 0.48837879742427504 1105 34-35 0.5153850778313185 1105 36-37 0.43977776046229167 1105 38-39 0.3968702315659485 1105 40-41 0.47681021326655326 1105 42-43 0.3450346622210887 1105 44-45 0.2219220377492448 1105 46-47 0.08868618583138499 1105 48-49 0.282649354693735 1105 50-51 0.3443291138066833 1105 52-53 0.32286011150936744 1105 54-55 0.31721100240067557 1105 56-57 0.36822301606846963 1105 58-59 0.4814164906497602 1105 60-61 0.2672622228891903 1105 62-63 0.2034077886598631 1105 64-65 0.44649038766500837 1105 66-67 0.5136576423904877 1105 68-69 0.016573452904264485 1105 70-71 -0.14849989214792814 1105 72-73 -0.27857443612568034 1105 74-75 -0.3613349120351508 1105 76-77 -0.1210868483909735 1106 1 0.11699880408970387 1106 2 0.3641991331574914 1106 3 -0.036499616824713144 1106 4 0.2438569163061075 1106 5 -0.03141908185762787 1106 6 0.27600743511094805 1106 7 0.3088417267550412 1106 8 0.28933057273761875 1106 9 0.17683220624659413 1106 10-11 0.1431984464037015 1106 12-13 0.14617546610620025 1106 14-15 0.23581501884734735 1106 16-17 0.2341890241892841 1106 18-19 0.2943259321169265 1106 20-21 0.2792973396310643 1106 22-23 0.17953078518230114 1106 24-25 0.38272825516932585 1106 26-27 0.26502130669697266 1106 28-29 0.06386902290815755 1106 30-31 0.09091397498768572 1106 32-33 0.1523174841965158 1106 34-35 0.1875817670893838 1106 36-37 0.23950908081354072 1106 38-39 0.23448905186669577 1106 40-41 0.20119963278923336 1106 42-43 0.16164405365377377 1106 44-45 0.13104832560039625 1106 46-47 0.2362189518885387 1106 48-49 0.2821497674255333 1106 50-51 0.08968722765602521 1106 52-53 0.10676875571570221 1106 54-55 0.3252865410729058 1106 56-57 0.29329014389952235 1106 58-59 -0.01705613237191983 1106 60-61 0.09946334918242528 1106 62-63 -0.037251545340481584 1106 64-65 -0.09861217585058313 1106 66-67 -0.09854514694931638 1106 68-69 -0.1336979536022227 1106 70-71 -0.20127822352645452 1106 72-73 -0.11233828755300834 1106 74-75 0.14054746142181784 1106 76-77 -0.11863318140695966 1107 1 -0.162055665882896 1107 2 -0.21911415076898777 1107 3 -0.17163219246110373 1107 4 -0.08803584581536228 1107 5 0.015611387695216195 1107 6 -0.17867088068769021 1107 7 -0.2028427049658461 1107 8 -0.030237243101325362 1107 9 -0.08544091018627853 1107 10-11 0.08593734041870249 1107 12-13 0.11984846198669175 1107 14-15 0.08614209079357948 1107 16-17 0.086926824018569 1107 18-19 -0.007727713697072147 1107 20-21 -0.13063789865186948 1107 22-23 -0.2867927700126529 1107 24-25 -0.42449157889836187 1107 26-27 -0.3047779838099771 1107 28-29 -0.20035059232240116 1107 30-31 0.1127708145442341 1107 32-33 0.13163673431457568 1107 34-35 0.020370353772570127 1107 36-37 0.09632032289989212 1107 38-39 -0.04469835207211048 1107 40-41 0.17386610578716244 1107 42-43 0.23947105201441587 1107 44-45 0.22442331904167645 1107 46-47 0.2771494960507681 1107 48-49 0.405365316892329 1107 50-51 0.3367532422477275 1107 52-53 0.2364096068514172 1107 54-55 0.1823519733786405 1107 56-57 0.28930164500714284 1107 58-59 0.33303690540756037 1107 60-61 0.18001829330216879 1107 62-63 0.22232545909896828 1107 64-65 0.209803264069663 1107 66-67 0.26137700589627144 1107 68-69 0.21866312000512167 1107 70-71 0.2764389403441747 1107 72-73 0.23753191386674644 1107 74-75 0.0777544787995339 1107 76-77 0.17880888753825985 1108 1 -0.07231726290581264 1108 2 0.07357334006239569 1108 3 0.04852404236952168 1108 4 -0.015078890174180515 1108 5 -0.029644159186410945 1108 6 0.017470994283407038 1108 7 -0.09964331184815478 1108 8 0.11888227835102327 1108 9 -0.022935646364288687 1108 10-11 -0.017209988714192548 1108 12-13 0.04201188593484062 1108 14-15 0.13309124847486942 1108 16-17 0.09430138120671927 1108 18-19 0.05795605639693946 1108 20-21 -0.04491623210209639 1108 22-23 0.030841638523632753 1108 24-25 0.1856361524318757 1108 26-27 -0.04311618716760535 1108 28-29 0.13003811432552936 1108 30-31 0.23512684461026367 1108 32-33 0.1753099954463906 1108 34-35 0.3177910733397127 1108 36-37 0.12299270231281412 1108 38-39 0.22239938397144243 1108 40-41 0.2220040067449247 1108 42-43 0.10438951215774495 1108 44-45 0.04870170455588152 1108 46-47 0.010133224830482845 1108 48-49 -0.03866130556691161 1108 50-51 0.009679633267243304 1108 52-53 -0.01985882035379305 1108 54-55 0.059261994256299744 1108 56-57 -0.05563772035738168 1108 58-59 -0.08916127890690717 1108 60-61 -0.10877894595909865 1108 62-63 -0.06696531611232714 1108 64-65 -0.24952955740323546 1108 66-67 -0.22322935822147372 1108 68-69 0.008357697357350702 1108 70-71 -0.0614414674577084 1108 72-73 -0.01199621798866346 1108 74-75 0.03851324093848518 1108 76-77 0.012144415725853008 1109 1 0.031513941980357174 1109 2 0.038592773710369954 1109 3 -0.08306951676429719 1109 4 0.16759740077529273 1109 5 0.15525312010286996 1109 6 0.04634384270095637 1109 7 -0.05799978047669896 1109 8 0.23881564870082883 1109 9 0.19138972842749524 1109 10-11 -0.13547761780913703 1109 12-13 0.07810294645676663 1109 14-15 0.19833278095682516 1109 16-17 0.0923580164093849 1109 18-19 -9.001346080523831E-4 1109 20-21 -0.041307126049900944 1109 22-23 0.23045296556416872 1109 24-25 0.12733520851183044 1109 26-27 0.2322863669689852 1109 28-29 0.25302534364256957 1109 30-31 0.24234505671464746 1109 32-33 0.22056263287004185 1109 34-35 0.3036322726734184 1109 36-37 0.18073839914789858 1109 38-39 0.2609890563756707 1109 40-41 0.24254814888817577 1109 42-43 0.22932010627212662 1109 44-45 0.10256067179630435 1109 46-47 -0.015130517534871046 1109 48-49 0.16955635129038882 1109 50-51 0.13738646280638633 1109 52-53 0.41489964716425476 1109 54-55 0.32605821857612227 1109 56-57 0.19005911473423254 1109 58-59 0.12821795485211496 1109 60-61 0.07195398019304022 1109 62-63 0.08017516140016667 1109 64-65 0.15080359084773676 1109 66-67 0.1371259999128931 1109 68-69 0.29791905216023906 1109 70-71 0.235893346534521 1109 72-73 0.2287033933383782 1109 74-75 0.31502628924497955 1109 76-77 0.49992897985688955 1110 1 -0.42123919458273207 1110 2 -0.3108016280582646 1110 3 -0.3069833237627364 1110 4 -0.3226983083743349 1110 5 0.00522127719261789 1110 6 -0.33768835871529035 1110 7 -0.41996054593070653 1110 8 -0.25665507986786906 1110 9 -0.1820560533706015 1110 10-11 -0.16958815132726812 1110 12-13 -0.2316433158268012 1110 14-15 -0.35003900433014934 1110 16-17 -0.31718101192279136 1110 18-19 -0.23105558921842118 1110 20-21 -0.36263240319981094 1110 22-23 -0.36656006744023983 1110 24-25 -0.15105693583492297 1110 26-27 -0.12539331229931605 1110 28-29 -0.05383990036738595 1110 30-31 -0.11729984747243805 1110 32-33 0.06145694501217491 1110 34-35 0.2373330017813302 1110 36-37 0.19809635572204343 1110 38-39 0.09791065577874036 1110 40-41 0.08374952286967385 1110 42-43 0.03736827432108214 1110 44-45 -0.03333408483496214 1110 46-47 0.02362631193140885 1110 48-49 -0.13401967311133234 1110 50-51 -0.13203589077087585 1110 52-53 -0.1364850457957658 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8 -1.0610308833236672 2314 9 -0.8412690330463732 2314 10-11 -0.9456236934119673 2314 12-13 -0.8788036543296514 2314 14-15 -0.9565504067008135 2314 16-17 -1.4881531889707915 2314 18-19 -1.204452758194634 2314 20-21 -1.0667493136881276 2314 22-23 -0.8811804312247702 2314 24-25 -0.841559864911595 2314 26-27 -0.8322265139820644 2314 28-29 -0.8781658314825549 2314 30-31 -1.0676896166133076 2314 32-33 -1.2267632462564677 2314 34-35 -1.206038301726288 2314 36-37 -1.1818008972484364 2314 38-39 -1.1390688627256154 2314 40-41 -0.885317592719538 2314 42-43 -0.961318674876086 2314 44-45 -1.0932455870456224 2314 46-47 -1.122473792555077 2314 48-49 -0.9667462223289967 2314 50-51 -0.8525631364856814 2314 52-53 -0.7367943353234061 2314 54-55 -0.8801699499175477 2314 56-57 -0.6746505096913964 2314 58-59 -0.7160710276897362 2314 60-61 -0.5740559658625521 2314 62-63 -0.5961437646617398 2314 64-65 -0.5452360582804658 2314 66-67 -0.4643506347201587 2314 68-69 -0.4362610056731704 2314 70-71 -0.29744441432512403 2314 72-73 -0.34042751517237946 2314 74-75 -0.3791734580583679 2314 76-77 -0.42455552627943405 2315 1 -1.2483402897044655 2315 2 -1.1577778249991653 2315 3 -1.3328390573132864 2315 4 -1.0450152601346758 2315 5 -0.9795266637975146 2315 6 -1.1150329147837112 2315 7 -1.1308918519312527 2315 8 -1.173925421075218 2315 9 -1.05804845087264 2315 10-11 -0.9965860696909132 2315 12-13 -1.1449603580930727 2315 14-15 -1.343937094655061 2315 16-17 -1.2436069328206045 2315 18-19 -1.254100208640999 2315 20-21 -1.2979279050551753 2315 22-23 -1.4225615155350937 2315 24-25 -1.4959895089184627 2315 26-27 -1.4480940091949677 2315 28-29 -1.4478556483988783 2315 30-31 -1.4080139392454498 2315 32-33 -1.526842448598103 2315 34-35 -1.5432697677000675 2315 36-37 -1.7277937700811918 2315 38-39 -1.4625364435062593 2315 40-41 -1.411002943416861 2315 42-43 -1.5406043928886177 2315 44-45 -1.6622887413649678 2315 46-47 -1.6926412235921688 2315 48-49 -1.4784092106260402 2315 50-51 -1.3736849087815557 2315 52-53 -1.4767981963071257 2315 54-55 -1.4520545795500759 2315 56-57 -1.6171980284876035 2315 58-59 -1.5083741044763528 2315 60-61 -1.6168466222819582 2315 62-63 -1.6397208095482831 2315 64-65 -1.5958463545848787 2315 66-67 -1.5044109495468554 2315 68-69 -1.3736302148497863 2315 70-71 -1.71282594734436 2315 72-73 -2.828833607178577 2315 74-75 -3.291985141186391 2315 76-77 -2.8353327840418387 2316 1 -3.496918785266807 2316 2 -3.3511431676442953 2316 3 -2.73379351679241 2316 4 -2.864156828590957 2316 5 -3.4568844406053145 2316 6 -3.0740609956799716 2316 7 -3.435824195885857 2316 8 -3.2702967290920952 2316 9 -2.8896038124323695 2316 10-11 -2.8774183117977117 2316 12-13 -2.952313769994717 2316 14-15 -3.1237304890970847 2316 16-17 -4.533858642408852 2316 18-19 -3.965830166446903 2316 20-21 -3.510103083288847 2316 22-23 -3.7190986899858416 2316 24-25 -3.8028918805174783 2316 26-27 -3.4954939800955884 2316 28-29 -3.5229611336309503 2316 30-31 -3.8217372041952586 2316 32-33 -3.874236599623856 2316 34-35 -3.876256818478474 2316 36-37 -3.7867170932297505 2316 38-39 -3.6865114180443115 2316 40-41 -3.6279824283579387 2316 42-43 -3.829741596438744 2316 44-45 -3.684610871439169 2316 46-47 -3.251407410119157 2316 48-49 -3.4799049184683213 2316 50-51 -3.879295268158831 2316 52-53 -4.984713226133984 2316 54-55 -4.0560033646512 2316 56-57 -3.989213160162276 2316 58-59 -3.800322307589987 2316 60-61 -3.747109971619949 2316 62-63 -3.7292333950276984 2316 64-65 -3.703746834724555 2316 66-67 -3.860406875634446 2316 68-69 -3.776647819971277 2316 70-71 -3.52340647986291 2316 72-73 -3.480872018352734 2316 74-75 -3.6434648443727724 2316 76-77 -3.6666611704815324 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42457.0 3 4136.0 4 5236.0 5 6200.0 6 7220.0 7 7813.0 8 8847.0 9 9156.0 10 10710.0 11 11442.0 12 9490.0 13 8324.0 14 8452.0 15 8337.0 16 9167.0 17 9491.0 18 9829.0 19 10668.0 20 11076.0 21 12120.0 22 13522.0 23 15342.0 24 18311.0 25 21123.0 26 25229.0 27 29890.0 28 34694.0 29 41579.0 30 49111.0 31 58832.0 32 71342.0 33 89384.0 34 118297.0 35 157847.0 36 223509.0 37 305265.0 38 280320.0 39 77314.0 40 608.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.587753714521238 26.82693190130378 28.665878536336624 21.919435847838354 2 22.193796231079233 26.953903734545293 28.673220634626695 22.179079399748783 3 22.22740538080648 27.10034679494351 28.598364101021268 22.073883723228743 4 22.09991196920171 27.16040666861808 28.64802397739547 22.09165738478474 5 22.438620680666798 27.138535259269958 28.603026187387282 21.81981787267596 6 22.607355533375546 27.006545757855832 28.45922178574182 21.926876923026796 7 22.417485023566396 26.813884922990365 28.287932016685197 22.48069803675804 8 22.22592720718294 27.331873183071455 28.28486289775563 22.157336711989977 9 22.304063726672187 26.74696583619534 28.406985256581535 22.541985180550938 10-11 22.730736144209278 27.111605562084673 28.065837408299682 22.09182088540637 12-13 22.654820831241377 26.77705860039096 28.278542407223718 22.289578161143947 14-15 22.37336188934509 27.043725125941677 28.39504932591268 22.18786365880055 16-17 22.760937312489446 26.9227281390862 28.23783384005869 22.078500708365663 18-19 22.617974718122515 26.87407150753099 28.05420563133143 22.453748143015062 20-21 22.655763106634762 27.060527629982833 27.89521263870117 22.388496624681224 22-23 22.684403854577074 27.02996782421469 27.612303646652364 22.673324674555875 24-25 22.623233580283387 27.139370145663694 27.697716406696138 22.539679867356785 26-27 22.625748311927776 27.229117531646686 27.625918035658277 22.51921612076726 28-29 22.86196263466121 27.16294885826623 27.429060620862284 22.546027886210275 30-31 22.62966319688996 27.01717764825924 27.582552683352514 22.77060647149829 32-33 22.585254000513174 27.281117251996694 27.52486108339341 22.608767664096728 34-35 22.51858030503957 27.09357653087269 27.592825498814534 22.795017665273214 36-37 22.50828347949854 26.928266140057396 27.94742668411291 22.61602369633116 38-39 22.51706770304274 27.08757089990233 27.758766145518955 22.63659525153598 40-41 22.355295762119574 27.073772321246746 27.650297616623305 22.920634300010374 42-43 22.349099805447736 27.214942917306463 27.4752872463048 22.960670030941007 44-45 22.44693537753729 26.976828927889894 27.68885553324203 22.88738016133079 46-47 22.280133672166457 26.918197218041723 27.655479706129793 23.146189403662028 48-49 22.40679393850685 26.9028928767435 27.64324056289561 23.04707262185404 50-51 22.23995393857753 27.03148254771811 27.600189025648824 23.128374488055535 52-53 22.353285312275844 26.887084425060504 27.642708557122127 23.116921705541525 54-55 22.33151872533157 27.053708812173138 27.571885036083053 23.042887426412243 56-57 22.316163182644306 26.86732890291652 27.59956150224313 23.216946412196037 58-59 22.356822541819916 26.90431484038444 27.62679026978444 23.112072348011203 60-61 22.31006208953765 26.88624974033186 27.523220781054615 23.28046738907588 62-63 22.236123180534435 26.785275907017166 27.7169780577884 23.261622854660004 64-65 22.226069368666977 26.6431547180182 27.694349012582343 23.43642690073249 66-67 22.06081956341441 26.703839065888673 27.8427733521042 23.392568018592716 68-69 22.332183712614015 26.469378786537217 27.82380002792117 23.3746374729276 70-71 22.029982137191585 26.42375492748395 27.922698805234543 23.62356413008992 72-73 22.08924498432406 26.264604655990688 27.9458078204166 23.70034253926865 74-75 22.03864519282789 26.299854980668165 27.785535510443864 23.875964316060085 76-77 21.898465363686388 26.174468923444817 28.097879769838517 23.82918594303028 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5159.0 1 3040.0 2 911.5 3 896.5 4 891.0 5 951.5 6 1037.5 7 1127.0 8 1184.0 9 1177.0 10 1238.5 11 1360.5 12 1484.0 13 1547.0 14 1652.5 15 1920.0 16 2238.0 17 2394.0 18 2534.5 19 3064.0 20 3856.0 21 4853.5 22 5448.0 23 6371.0 24 8565.0 25 11656.0 26 13476.0 27 15839.0 28 21255.5 29 26450.0 30 28591.0 31 32087.5 32 39006.5 33 45992.0 34 52824.5 35 56094.0 36 59087.5 37 65238.5 38 69346.0 39 70296.0 40 73173.5 41 77766.5 42 82368.0 43 85254.0 44 85675.0 45 87155.0 46 89008.5 47 90774.0 48 91745.0 49 91228.5 50 89547.5 51 85589.5 52 82796.0 53 80198.0 54 74968.5 55 67231.5 56 57654.0 57 53182.0 58 49759.5 59 41730.0 60 33909.5 61 30696.0 62 26046.0 63 18553.5 64 13520.5 65 11330.0 66 9831.0 67 7343.5 68 5452.0 69 3738.5 70 2928.0 71 2518.5 72 1860.0 73 1457.0 74 1303.0 75 1133.5 76 828.5 77 582.0 78 471.0 79 371.5 80 233.0 81 168.0 82 108.0 83 74.0 84 75.0 85 61.5 86 53.0 87 59.0 88 47.5 89 33.5 90 30.5 91 30.0 92 27.0 93 28.5 94 28.5 95 27.0 96 30.0 97 27.5 98 30.5 99 54.0 100 72.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010913888873806123 2 0.014171766149569144 3 0.013900276376588893 4 0.015692108878258556 5 0.012542827511687634 6 0.010425207282441671 7 0.016126492515026958 8 0.01775543115290847 9 0.01770113319831242 10-11 0.019873051382154432 12-13 0.016913812856669687 14-15 0.00885056659915621 16-17 0.006895840233698396 18-19 0.017999771948590697 20-21 0.024787016273096995 22-23 0.02163773490652607 24-25 0.01952011467728011 26-27 0.025112804000673294 28-29 0.009257801258626587 30-31 0.009176354326732512 32-33 0.011266825578680452 34-35 0.012162741829515282 36-37 0.012135592852217257 38-39 0.014850490582019775 40-41 0.012325635693303435 42-43 0.015339172173384229 44-45 0.013900276376588893 46-47 0.02546574070554762 48-49 0.02500420809148119 50-51 0.03637962957935374 52-53 0.041347892424892355 54-55 0.028289234344542242 56-57 0.02190922467950632 58-59 0.01696811081126574 60-61 0.021583436951930022 62-63 0.02660599775206468 64-65 0.027067530366131104 66-67 0.02926659752727115 68-69 0.04305827799466794 70-71 0.053972166868474065 72-73 0.08736540894504503 74-75 0.0863608967850181 76-77 0.09108481883487449 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1841690.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.28837172653631 #Duplication Level Percentage of deduplicated Percentage of total 1 71.42016260594575 38.05864173715305 2 14.003033077950192 14.923976639135875 3 5.791354607774143 9.258355714177725 4 2.995950437531779 6.385972823578902 5 1.7342243315060106 4.620699541724812 6 1.0518964882226016 3.3632310649346477 7 0.6950900452286148 2.5928151699490156 8 0.48657803268616784 2.0743160863797803 9 0.3339933896194333 1.601816751022161 >10 1.4076743314242213 12.431746046693272 >50 0.05557525193372083 2.0131292542787618 >100 0.024160375148894745 2.270313254487571 >500 1.023416761747674E-4 0.03157646722925212 >1k 2.0468335234953477E-4 0.37340944925507635 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4769 0.2589469454685642 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2078 0.11283114965059265 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.4297954596050365E-5 0.0 2 0.0 0.0 0.0 4.343836367684029E-4 0.0 3 0.0 0.0 0.0 7.601713643447051E-4 0.0 4 0.0 0.0 0.0 0.0017375345470736117 0.0 5 0.0 0.0 0.0 0.0034750690941472233 0.0 6 0.0 0.0 0.0 0.006895840233698397 0.0 7 0.0 0.0 0.0 0.014551851831741498 0.0 8 0.0 0.0 0.0 0.02372820615847401 0.0 9 0.0 0.0 0.0 0.035945245942585344 0.0 10 0.0 0.0 0.0 0.04903105300023348 5.4297954596050365E-5 11 0.0 0.0 0.0 0.06542903528824069 5.4297954596050365E-5 12 0.0 0.0 0.0 0.08584506621635563 5.4297954596050365E-5 13 0.0 0.0 0.0 0.11353702306034132 5.4297954596050365E-5 14 0.0 0.0 0.0 0.14394387763412952 1.0859590919210073E-4 15 0.0 0.0 0.0 0.17782580130206496 1.0859590919210073E-4 16 0.0 0.0 0.0 0.21002448837752283 1.0859590919210073E-4 17 0.0 0.0 0.0 0.2426575590897491 1.0859590919210073E-4 18 0.0 0.0 0.0 0.2784399111685463 1.0859590919210073E-4 19 0.0 0.0 0.0 0.31818601393285517 1.0859590919210073E-4 20 0.0 0.0 0.0 0.36162437760969546 1.0859590919210073E-4 21 0.0 0.0 0.0 0.4103839408369487 1.0859590919210073E-4 22 0.0 0.0 0.0 0.46413891588703854 1.0859590919210073E-4 23 0.0 0.0 0.0 0.5168079318452073 1.628938637881511E-4 24 0.0 0.0 0.0 0.5687710743936276 1.628938637881511E-4 25 0.0 0.0 0.0 0.6213314944426044 1.628938637881511E-4 26 0.0 0.0 0.0 0.6780185590408809 1.628938637881511E-4 27 0.0 0.0 0.0 0.7300902974984932 1.628938637881511E-4 28 0.0 0.0 0.0 0.7842796561853514 3.800856821723526E-4 29 0.0 0.0 0.0 0.8425413614669135 3.800856821723526E-4 30 0.0 0.0 0.0 0.9126943188050106 3.800856821723526E-4 31 0.0 0.0 0.0 0.9873540063745798 3.800856821723526E-4 32 0.0 0.0 0.0 1.0667919139486015 3.800856821723526E-4 33 0.0 0.0 0.0 1.1531256617563217 3.800856821723526E-4 34 0.0 0.0 0.0 1.2394051116094458 3.800856821723526E-4 35 0.0 0.0 0.0 1.3266619246452986 3.800856821723526E-4 36 0.0 0.0 0.0 1.4158734640466093 3.800856821723526E-4 37 0.0 0.0 0.0 1.5116550559540423 3.800856821723526E-4 38 0.0 0.0 0.0 1.6042873664949042 3.800856821723526E-4 39 0.0 0.0 0.0 1.702240876586179 3.800856821723526E-4 40 0.0 0.0 0.0 1.8114340632788364 3.800856821723526E-4 41 0.0 0.0 0.0 1.9261656413402908 3.800856821723526E-4 42 0.0 0.0 0.0 2.0636480623774904 3.800856821723526E-4 43 0.0 0.0 0.0 2.2027051240979754 3.800856821723526E-4 44 0.0 0.0 0.0 2.347843556733218 3.800856821723526E-4 45 0.0 0.0 0.0 2.4948824177793223 3.800856821723526E-4 46 0.0 0.0 0.0 2.6530523595176168 3.800856821723526E-4 47 0.0 0.0 0.0 2.8177380558074376 3.800856821723526E-4 48 0.0 0.0 0.0 2.9804690257318005 3.800856821723526E-4 49 0.0 0.0 0.0 3.148086811569808 3.800856821723526E-4 50 0.0 0.0 0.0 3.3277044453735427 3.800856821723526E-4 51 0.0 0.0 0.0 3.526543555104279 3.800856821723526E-4 52 0.0 0.0 0.0 3.7330929743876546 3.800856821723526E-4 53 0.0 0.0 0.0 3.9667913709690557 3.800856821723526E-4 54 0.0 0.0 0.0 4.206136754828446 3.800856821723526E-4 55 0.0 0.0 0.0 4.449445889373347 3.800856821723526E-4 56 0.0 0.0 0.0 4.701171206880637 3.800856821723526E-4 57 0.0 0.0 0.0 4.958652107575108 3.800856821723526E-4 58 0.0 0.0 0.0 5.229490305100207 3.800856821723526E-4 59 0.0 0.0 0.0 5.506409873540064 3.800856821723526E-4 60 0.0 0.0 0.0 5.791962816760693 3.800856821723526E-4 61 0.0 0.0 0.0 6.086257730671286 3.800856821723526E-4 62 0.0 0.0 0.0 6.403955063012776 3.800856821723526E-4 63 0.0 0.0 0.0 6.735715565594644 3.800856821723526E-4 64 0.0 0.0 0.0 7.079475916142239 3.800856821723526E-4 65 0.0 0.0 0.0 7.445064044437446 3.800856821723526E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCGCA 95 0.0066484422 18.68274 40 CTAAGCG 145 2.1934023E-4 17.134253 11 TAAGCGT 125 0.0016046254 17.038195 12 AACGTAC 280 3.2359821E-9 16.484873 51 CTCGAAC 630 0.0 15.779106 23 TCGAACT 635 0.0 15.654435 24 CCTCGAA 670 0.0 15.366965 22 GCCTCGA 630 0.0 15.215153 21 CGAACTC 680 0.0 15.139336 25 AAGGTCG 425 1.8189894E-12 15.035336 54 CCCGATA 145 0.00431806 14.688098 38 CGATACT 200 1.5576255E-4 14.197725 31 AATCCGC 505 0.0 14.057535 35 CGGCTAG 310 2.0128391E-7 13.738987 3 CGTGCGC 340 4.1931344E-8 13.575041 46 AGGTCGG 450 5.4569682E-11 13.410055 55 ATTCGAT 160 0.008281865 13.313259 61 TAAGGCG 485 1.4551915E-11 13.176367 22 AGTCGTG 325 3.5779158E-7 13.104879 11 GGCCTCG 825 0.0 12.909826 20 >>END_MODULE