FastQCFastQC Report
Thu 26 May 2016
SRR936119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936119_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1841690
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44400.24108291840646362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36070.19585272222795366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23340.12673142602718154No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA7750.044.5445100-101
GGTATCA51750.038.864781
TCGAGTA2600.037.77273100-101
CCTGTCG6250.037.13686496-97
CGGTATC6950.036.397533100-101
CGTATAG1600.035.340397100-101
CGAGTAC4850.032.521606100-101
AGTATCA20050.031.170425100-101
CCGAGCG5050.029.46247796-97
TCGTATC3950.029.383621100-101
TTATACG5900.029.25274396-97
GTATCAA96250.028.0057581
CGTACAT11150.027.75852100-101
TTCGCGA656.382272E-527.46138290-91
TATACGT2950.027.23531396-97
AACGTAT2200.027.0548598-99
CCGTATA2100.026.926018100-101
ATACACG7000.026.78139396-97
CACGTAT2650.025.82972598-99
TCGGTAT2650.025.829725100-101
Produced by FastQC (version 0.11.5)