FastQCFastQC Report
Thu 26 May 2016
SRR936118_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936118_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1839664
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48310.2626023012898008No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20460.111215961175519No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCTA750.00169051823.66110229
GTTACCG1250.001603383617.04016342
CCGTATG1300.002081587416.38789252
GCGGATT1759.156593E-414.19820510
GCGTAAG1550.006716475413.741693538
TGCGAAA1900.001699635313.08085250
AGTCGTG3801.2765668E-813.07729411
GACGCGA2801.4576268E-512.68455769
GGCGGAT2558.610502E-512.5278289
ACCGTTC2601.0307905E-412.28891339
GTCGTGT4307.140261E-811.55762212
CGGATTT2150.004302482611.5566811
ACGCGAC3104.130239E-511.45826570
CGTGTGA4409.8265446E-811.29433414
TCGCGTC3205.74003E-511.09415858
TCGAACT6401.2732926E-1111.09415824
CGAACTC6451.6370905E-1111.00725825
CCGGGGT4601.8107312E-710.80562748
GCCTCGA7007.2759576E-1210.65039221
CACGACT3359.164826E-510.5948118
Produced by FastQC (version 0.11.5)