##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936118_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1839664 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.645089538089564 39.0 36.0 41.0 30.0 41.0 2 36.51044592925665 39.0 36.0 41.0 30.0 41.0 3 36.37089979474513 39.0 36.0 41.0 29.0 41.0 4 36.258617878047296 39.0 36.0 41.0 29.0 41.0 5 36.18905137025022 39.0 36.0 41.0 29.0 41.0 6 36.09819184372798 39.0 35.0 40.0 29.0 41.0 7 35.95541794588577 39.0 35.0 40.0 29.0 41.0 8 35.81847065551101 39.0 35.0 40.0 28.0 41.0 9 35.98150531836249 39.0 35.0 40.0 29.0 41.0 10-11 36.18445188904061 39.0 35.0 41.0 29.5 41.0 12-13 36.092978663495074 39.0 35.0 41.0 29.0 41.0 14-15 35.93640632202402 39.0 35.0 41.0 28.0 41.0 16-17 35.78168024160934 39.0 35.0 41.0 27.0 41.0 18-19 35.628718613833826 39.0 35.0 41.0 27.0 41.0 20-21 35.42370753572391 39.0 35.0 41.0 26.5 41.0 22-23 35.16966766757408 39.0 35.0 41.0 25.5 41.0 24-25 34.950459431722315 39.0 34.0 41.0 24.0 41.0 26-27 33.98149444681202 38.0 33.0 40.0 23.0 40.5 28-29 33.99420763791649 38.0 33.0 39.5 23.0 40.5 30-31 34.40851345680515 38.0 34.0 40.0 23.0 41.0 32-33 34.28639333052122 38.0 34.0 40.0 23.0 41.0 34-35 34.03363820784665 38.0 33.5 40.0 22.0 41.0 36-37 33.735260351890346 37.5 33.0 40.0 21.5 41.0 38-39 33.43070473738683 37.0 33.0 40.0 20.0 41.0 40-41 33.08121917915446 36.5 32.5 40.0 18.5 41.0 42-43 32.71567172048809 36.0 32.0 39.0 18.0 41.0 44-45 32.35381759930074 36.0 32.0 39.0 14.5 41.0 46-47 31.942008431974532 35.0 31.0 39.0 11.5 40.0 48-49 31.5263974834535 35.0 31.0 38.0 7.5 40.0 50-51 31.076672968542084 35.0 31.0 37.0 6.5 39.0 52-53 30.595041268405534 35.0 30.0 36.5 2.0 39.0 54-55 30.130087885613897 34.5 30.0 36.0 2.0 38.5 56-57 29.688755392289025 34.0 29.5 36.0 2.0 37.0 58-59 29.260386135729135 34.0 29.0 35.0 2.0 37.0 60-61 28.901665195383504 34.0 29.0 35.0 2.0 36.5 62-63 28.539598263595963 34.0 29.0 35.0 2.0 36.0 64-65 28.157474136581463 34.0 28.5 35.0 2.0 36.0 66-67 27.80323716722184 34.0 27.5 35.0 2.0 35.0 68-69 27.435188708372834 34.0 27.0 35.0 2.0 35.0 70-71 26.99339172805469 33.0 26.0 35.0 2.0 35.0 72-73 26.526371663521168 33.0 25.0 35.0 2.0 35.0 74-75 25.962782877742892 33.0 24.5 35.0 2.0 35.0 76-77 24.204124231381382 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.2511372569131112 1101 2 1.0203039991686893 1101 3 1.1288104199712734 1101 4 0.9762272037256707 1101 5 1.036940616213677 1101 6 1.226415402494517 1101 7 1.2557608507397262 1101 8 1.0142353885223017 1101 9 1.1861916132974102 1101 10-11 1.140797524554479 1101 12-13 1.2664652079059309 1101 14-15 1.311145415054476 1101 16-17 1.3228583932050029 1101 18-19 1.3378283106067599 1101 20-21 1.18553007086188 1101 22-23 1.0526855767548469 1101 24-25 1.046886422753424 1101 26-27 1.0920927593768681 1101 28-29 1.0996619243128336 1101 30-31 1.0052031126354848 1101 32-33 1.1803898814183214 1101 34-35 1.1320723377781121 1101 36-37 1.1657441815623244 1101 38-39 1.1204778124609476 1101 40-41 0.9828743178046366 1101 42-43 1.1214255279365375 1101 44-45 1.0898282833874546 1101 46-47 1.023241520767364 1101 48-49 1.1354391682447051 1101 50-51 1.0962016330694055 1101 52-53 1.2232313345876378 1101 54-55 1.2153257145410166 1101 56-57 1.2081764754158577 1101 58-59 1.2586390666287564 1101 60-61 1.3260920958080433 1101 62-63 1.251375346346208 1101 64-65 1.2637814616306358 1101 66-67 1.4153077060421566 1101 68-69 1.3822707459222308 1101 70-71 1.2559363079396988 1101 72-73 1.232930442841976 1101 74-75 1.2430668983208335 1101 76-77 1.5263919237821248 1102 1 0.7948936496483867 1102 2 0.8109080750477702 1102 3 0.7749644644776055 1102 4 0.8001186282204884 1102 5 0.7360179135156244 1102 6 0.7494958628574082 1102 7 0.8258982891760738 1102 8 0.806691746016952 1102 9 0.6627114726787227 1102 10-11 0.7329141538317074 1102 12-13 0.8022494837110941 1102 14-15 1.006537488492505 1102 16-17 0.9802324939244329 1102 18-19 0.9679188618217083 1102 20-21 1.18248304611609 1102 22-23 1.1008459731688944 1102 24-25 1.2221720007426455 1102 26-27 0.9537969444159344 1102 28-29 1.077789099991648 1102 30-31 1.1566507717125205 1102 32-33 1.0051773632608132 1102 34-35 1.0824647120507436 1102 36-37 1.1971674300603112 1102 38-39 1.17375562032219 1102 40-41 1.199794330181824 1102 42-43 1.1647145526307554 1102 44-45 1.0831776898837688 1102 46-47 1.0365083266774704 1102 48-49 1.1533420502401803 1102 50-51 1.1741220924008644 1102 52-53 1.3355522305590313 1102 54-55 1.2529031794508114 1102 56-57 1.30644409787703 1102 58-59 1.1423181448715347 1102 60-61 1.0110000833964676 1102 62-63 0.9895127933778305 1102 64-65 0.8464443635671515 1102 66-67 0.973653502523252 1102 68-69 1.0463180027404313 1102 70-71 1.202799247695424 1102 72-73 0.9485600896621555 1102 74-75 0.7196286596644477 1102 76-77 0.8791860325811065 1103 1 0.5435305563498076 1103 2 0.44742376962798147 1103 3 0.39650242472524155 1103 4 0.46647031715281884 1103 5 0.4488125263102347 1103 6 0.4606830030768023 1103 7 0.5162860376283334 1103 8 0.2608787776233328 1103 9 0.4336683533130241 1103 10-11 0.5755990674843545 1103 12-13 0.5292126639000827 1103 14-15 0.5015847351526475 1103 16-17 0.40115136000458307 1103 18-19 0.40767665480530724 1103 20-21 0.4164463349180849 1103 22-23 0.40835113228695974 1103 24-25 0.587339807845936 1103 26-27 0.5078047645527803 1103 28-29 0.4680628047814821 1103 30-31 0.47948122680104177 1103 32-33 0.4599291825287537 1103 34-35 0.5402739876019567 1103 36-37 0.6344434157233891 1103 38-39 0.5459832731950058 1103 40-41 0.6148969694780178 1103 42-43 0.5577144440177477 1103 44-45 0.5312823928271868 1103 46-47 0.46236365414569747 1103 48-49 0.5545367933704064 1103 50-51 0.5409951237900259 1103 52-53 0.5899401962373219 1103 54-55 0.618842138938156 1103 56-57 0.5498187041548626 1103 58-59 0.582130244361057 1103 60-61 0.5280794795081221 1103 62-63 0.575985042753306 1103 64-65 0.5774153638929924 1103 66-67 0.577607016154186 1103 68-69 0.5190995819736202 1103 70-71 0.45181086928769076 1103 72-73 0.6443350435016235 1103 74-75 0.5379625360628424 1103 76-77 0.5575611819239974 1104 1 0.3230693306312986 1104 2 0.4183336723159812 1104 3 0.4663424704554586 1104 4 0.5605044186319503 1104 5 0.49703828202566314 1104 6 0.5753217445489014 1104 7 0.4792388157725398 1104 8 0.4942188757809376 1104 9 0.2782542953317133 1104 10-11 0.3903567007429416 1104 12-13 0.45558380921865904 1104 14-15 0.6678061072619599 1104 16-17 0.7383063116868342 1104 18-19 0.5895511537855143 1104 20-21 0.4366212394495008 1104 22-23 0.44820203556012217 1104 24-25 0.5677679873979073 1104 26-27 0.3354150565564211 1104 28-29 0.48964682220766775 1104 30-31 0.5999304449564775 1104 32-33 0.5138020444214817 1104 34-35 0.5161699040157828 1104 36-37 0.5712388169958516 1104 38-39 0.5648593314672041 1104 40-41 0.6217815311343173 1104 42-43 0.5842998651449705 1104 44-45 0.48517774776059497 1104 46-47 0.35616825896158133 1104 48-49 0.2699049520190684 1104 50-51 0.2466210898914909 1104 52-53 0.32517884232539274 1104 54-55 0.36754684421167383 1104 56-57 0.24306690217479954 1104 58-59 0.30394849382748035 1104 60-61 0.36986123274762406 1104 62-63 0.3260944637858181 1104 64-65 0.2661774687004126 1104 66-67 0.22463844214531647 1104 68-69 0.24689945817733872 1104 70-71 0.2028994986328172 1104 72-73 0.09919590797851541 1104 74-75 -0.03985797285488957 1104 76-77 -0.1490113522129981 1105 1 -0.28393977772191903 1105 2 -0.007236595761938247 1105 3 -0.11723872033142868 1105 4 0.015585071904517633 1105 5 -0.017699929560350824 1105 6 -0.09782056546950457 1105 7 -0.051696015024745634 1105 8 -0.21150542313188936 1105 9 -0.1611058601621025 1105 10-11 0.11927216273748087 1105 12-13 0.07936460526234157 1105 14-15 0.1671722583589954 1105 16-17 0.11349032706901596 1105 18-19 0.2510611444982018 1105 20-21 0.13429653900107041 1105 22-23 0.07154263154136942 1105 24-25 0.3547336896737505 1105 26-27 0.0635249001874385 1105 28-29 0.2323270266590427 1105 30-31 0.3746152839177057 1105 32-33 0.29449293646013075 1105 34-35 0.25483279306055096 1105 36-37 0.23464251702758787 1105 38-39 0.19619798377576814 1105 40-41 0.2539352719857959 1105 42-43 0.28966364412535484 1105 44-45 0.21147388876047302 1105 46-47 0.1728683651582088 1105 48-49 0.019357967520118535 1105 50-51 0.10781046604832767 1105 52-53 0.11087456088994685 1105 54-55 -0.07311725701605098 1105 56-57 0.012536611316196655 1105 58-59 -0.044747103257531506 1105 60-61 0.13182304898434438 1105 62-63 0.051481467564748584 1105 64-65 0.19951328387385914 1105 66-67 0.19453992615508042 1105 68-69 0.14560413357224533 1105 70-71 0.05838619401045975 1105 72-73 -0.5143822392886506 1105 74-75 -0.6325550276944121 1105 76-77 -0.4993178594671548 1106 1 0.16128244393091506 1106 2 0.27380346204182615 1106 3 0.1759622912344483 1106 4 0.1136163874013576 1106 5 0.02784743826735081 1106 6 0.24486082715026214 1106 7 -0.27562987378473025 1106 8 -0.011481594452405375 1106 9 0.11109965066841454 1106 10-11 -0.04742610468692021 1106 12-13 0.3599123958569308 1106 14-15 0.41244943970805537 1106 16-17 0.336661604030013 1106 18-19 0.37385378886668974 1106 20-21 0.39659040811244495 1106 22-23 0.3087235065919671 1106 24-25 0.20212829260595555 1106 26-27 -0.01412136434677791 1106 28-29 0.24808662346753607 1106 30-31 0.4774257869846963 1106 32-33 0.36642159903065874 1106 34-35 0.3754437040335219 1106 36-37 0.33752508263332714 1106 38-39 0.49516720122143454 1106 40-41 0.39090694147945015 1106 42-43 0.3512127388917534 1106 44-45 0.3343315495528074 1106 46-47 0.3550536728556395 1106 48-49 0.19623691014168188 1106 50-51 0.11033105351495465 1106 52-53 0.20329524725840287 1106 54-55 0.2386541106476585 1106 56-57 0.2754841677235511 1106 58-59 0.26145559168925203 1106 60-61 0.12573360432068625 1106 62-63 0.15420421447342747 1106 64-65 0.2722439224312261 1106 66-67 0.13816652306094213 1106 68-69 0.0628430301762748 1106 70-71 0.15778925996721682 1106 72-73 -0.20226913505226918 1106 74-75 -0.10445960536309684 1106 76-77 -0.033305114966967864 1107 1 0.0652314361152051 1107 2 0.17892774951905466 1107 3 0.18338623983486713 1107 4 0.12331991916371265 1107 5 0.08004768899701276 1107 6 -0.020824467946795266 1107 7 -0.003274777900223569 1107 8 0.09061973559010283 1107 9 0.01564363609475805 1107 10-11 0.1075708591335669 1107 12-13 0.19616560343366274 1107 14-15 -0.02971501348580574 1107 16-17 0.11253768872962411 1107 18-19 0.06788647186996144 1107 20-21 -0.11670022282524428 1107 22-23 -0.004611442772393559 1107 24-25 0.033543201226756025 1107 26-27 0.04273354544930186 1107 28-29 0.012836579393969316 1107 30-31 0.21121038473987852 1107 32-33 0.4465302159039837 1107 34-35 0.31724822304392575 1107 36-37 0.5031768328438986 1107 38-39 0.5840121797723015 1107 40-41 0.5757980307823019 1107 42-43 0.47963701069837583 1107 44-45 0.4726784967232831 1107 46-47 0.527710672718527 1107 48-49 0.555667920307986 1107 50-51 0.462633163170004 1107 52-53 0.3662266785721897 1107 54-55 0.40181937675410495 1107 56-57 0.45952928926867287 1107 58-59 0.3795066582697295 1107 60-61 0.31639465895233343 1107 62-63 0.3353918993375835 1107 64-65 0.4027592404465459 1107 66-67 0.46705332253597476 1107 68-69 0.33868483542984507 1107 70-71 0.3979584045481275 1107 72-73 0.35031186238166967 1107 74-75 0.03253207942270819 1107 76-77 0.23625130900122215 1108 1 0.2994560350456865 1108 2 0.303297497418221 1108 3 0.192776359519101 1108 4 0.09826262351818116 1108 5 0.14082563653966673 1108 6 0.1106053767183468 1108 7 0.12071936521157056 1108 8 0.30421153594660666 1108 9 0.18300421669062672 1108 10-11 0.20372568470016006 1108 12-13 0.3031302074570945 1108 14-15 0.015997362373624924 1108 16-17 0.02203285043066927 1108 18-19 0.08612240260589488 1108 20-21 -0.0051424178850822955 1108 22-23 -0.16190072212049955 1108 24-25 0.018674981211468378 1108 26-27 -0.13994407105183626 1108 28-29 -9.313794751619753E-5 1108 30-31 0.011239032562642137 1108 32-33 -0.09115758648970029 1108 34-35 0.016856702799465495 1108 36-37 0.11411393495748001 1108 38-39 0.11840866563937169 1108 40-41 0.14705153218286426 1108 42-43 0.16082812350713738 1108 44-45 -0.02914541465944609 1108 46-47 -0.01248540571284451 1108 48-49 -0.0429187871022485 1108 50-51 0.00793492023646536 1108 52-53 0.11343510739826712 1108 54-55 0.17956320155675343 1108 56-57 0.2100481331698667 1108 58-59 0.24164378584803003 1108 60-61 0.3335133246004105 1108 62-63 0.33592984178728713 1108 64-65 0.24125873115191965 1108 66-67 0.08718955884868151 1108 68-69 -0.02911501178176934 1108 70-71 0.003958531871784743 1108 72-73 0.04467142438829086 1108 74-75 0.12010847361674593 1108 76-77 0.17974507014204377 1109 1 -0.19603295451599934 1109 2 -0.23703947103331302 1109 3 -0.2610597799386625 1109 4 -0.16370582801486933 1109 5 -0.15066083101282146 1109 6 -0.3738013673210716 1109 7 -0.12658523745522388 1109 8 -0.1529954556233477 1109 9 -0.03416354362509111 1109 10-11 -0.2196163277697778 1109 12-13 -0.13036230824707928 1109 14-15 0.0025717499690713908 1109 16-17 -0.08727114508575795 1109 18-19 -0.03866918135599917 1109 20-21 -0.028763777901900767 1109 22-23 0.14282045575121316 1109 24-25 -0.022782296419705972 1109 26-27 -0.22178823189998553 1109 28-29 -0.005949219959518359 1109 30-31 0.09971134884128219 1109 32-33 0.03581711168651225 1109 34-35 0.17973617177641898 1109 36-37 0.161269377126402 1109 38-39 -0.023747418707827705 1109 40-41 0.1117273489168582 1109 42-43 0.06287346129290938 1109 44-45 0.10503685888514269 1109 46-47 0.0691126840987053 1109 48-49 0.06729739345492547 1109 50-51 0.022418083489856855 1109 52-53 0.15355607043214903 1109 54-55 0.2593001419859604 1109 56-57 0.1353052963765542 1109 58-59 0.08685226026598514 1109 60-61 0.03696357329853939 1109 62-63 0.037701961909650805 1109 64-65 0.09246982098681755 1109 66-67 0.05095377434035697 1109 68-69 -0.19425056977630106 1109 70-71 -0.2210502251046087 1109 72-73 -0.2245749502234844 1109 74-75 -0.07704229816476982 1109 76-77 -0.10873512741534697 1110 1 -0.3503893669228191 1110 2 -0.42824322520158375 1110 3 -0.6369179041447737 1110 4 -0.48876666974406646 1110 5 -0.6058389960618626 1110 6 -0.49095395214912685 1110 7 -0.5976122836615687 1110 8 -0.90623302533551 1110 9 -0.6447812039658487 1110 10-11 -0.7781653693178967 1110 12-13 -0.5375041484480079 1110 14-15 -0.5126389603998192 1110 16-17 -0.5604365500558757 1110 18-19 -0.6126202454745027 1110 20-21 -0.7121361059266889 1110 22-23 -0.6090100794557927 1110 24-25 -0.8464505632868864 1110 26-27 -0.8271738650210168 1110 28-29 -0.6037645347237017 1110 30-31 -0.43868400894590565 1110 32-33 -0.3354190710121969 1110 34-35 -0.4259369857869757 1110 36-37 -0.5912397880627864 1110 38-39 -0.5297871004177068 1110 40-41 -0.49550828431540594 1110 42-43 -0.4570751457985409 1110 44-45 -0.44636314044748815 1110 46-47 -0.5398382243005706 1110 48-49 -0.5605635303605503 1110 50-51 -0.707036571069267 1110 52-53 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-0.9444905714992871 2314 12-13 -0.9076583503902853 2314 14-15 -0.7901123542402999 2314 16-17 -0.9873412022206978 2314 18-19 -1.0766584673178343 2314 20-21 -1.0117553660946754 2314 22-23 -0.9081828713438185 2314 24-25 -0.8098885654193069 2314 26-27 -0.8013965308236237 2314 28-29 -1.0807472177612567 2314 30-31 -1.1539385536233482 2314 32-33 -1.1317938409915058 2314 34-35 -1.16004254174684 2314 36-37 -1.621704634894705 2314 38-39 -1.1025421389248748 2314 40-41 -0.9077800974386818 2314 42-43 -0.8449275120804316 2314 44-45 -0.8974333358885467 2314 46-47 -0.7024171359807099 2314 48-49 -0.6400649182819933 2314 50-51 -0.49641651730824776 2314 52-53 -0.47223188667209115 2314 54-55 -0.6240309633819514 2314 56-57 -0.5947713703891289 2314 58-59 -0.5685942487597515 2314 60-61 -0.628910432781332 2314 62-63 -0.6291386098357883 2314 64-65 -0.6028607348162858 2314 66-67 -0.6008862775464827 2314 68-69 -0.7920820167655798 2314 70-71 -0.5604599627988129 2314 72-73 -0.5755590920607112 2314 74-75 -0.5471087162895181 2314 76-77 -0.5797660070160937 2315 1 -1.10931816007448 2315 2 -0.9427685496259883 2315 3 -0.8321003047519042 2315 4 -0.8532058329884649 2315 5 -1.0428928291029607 2315 6 -0.9442348654460275 2315 7 -1.4388399151168372 2315 8 -1.2480718499693708 2315 9 -0.9280174294911419 2315 10-11 -1.0588527324608634 2315 12-13 -1.141559351945041 2315 14-15 -1.0670249415986959 2315 16-17 -0.9196156457870401 2315 18-19 -1.0430658351244873 2315 20-21 -1.0851669023112862 2315 22-23 -1.186076352198576 2315 24-25 -1.264610438982693 2315 26-27 -1.1056134947542233 2315 28-29 -1.218991216955466 2315 30-31 -1.3788271119320115 2315 32-33 -1.2591798295554923 2315 34-35 -1.3139601756189094 2315 36-37 -1.365005478390799 2315 38-39 -1.333237028877818 2315 40-41 -1.336229624554008 2315 42-43 -1.4314605003706582 2315 44-45 -1.4929462259344675 2315 46-47 -1.4376203136830767 2315 48-49 -1.1800212197091717 2315 50-51 -1.108637138282532 2315 52-53 -1.2215849308510371 2315 54-55 -1.4605084245258624 2315 56-57 -1.5086800166114784 2315 58-59 -1.3198791210989675 2315 60-61 -1.3629772409776564 2315 62-63 -1.4309041148228978 2315 64-65 -1.1887206824399854 2315 66-67 -1.3173388418675778 2315 68-69 -1.4817260748443921 2315 70-71 -1.4082556642228887 2315 72-73 -1.2166486638043708 2315 74-75 -1.2528076093623142 2315 76-77 -1.6293592383860904 2316 1 -1.9806279494275074 2316 2 -1.4426399455271124 2316 3 -1.601220893918729 2316 4 -2.526612286186605 2316 5 -1.9609024749739348 2316 6 -1.899780226850602 2316 7 -1.5302563523211816 2316 8 -1.2061261747753065 2316 9 -1.7033788678733188 2316 10-11 -1.5549601197012137 2316 12-13 -2.1782638183732885 2316 14-15 -3.2952808738995145 2316 16-17 -2.3879845603391345 2316 18-19 -1.8929676068403793 2316 20-21 -1.7057410784729825 2316 22-23 -1.902820132482809 2316 24-25 -2.029387342993992 2316 26-27 -1.9614730964985512 2316 28-29 -2.393463021549376 2316 30-31 -2.416926724316326 2316 32-33 -2.486848420963028 2316 34-35 -2.4140877653121002 2316 36-37 -2.552449052074415 2316 38-39 -2.7441083019231485 2316 40-41 -2.456280592804241 2316 42-43 -2.7019756719348393 2316 44-45 -2.6362134463163542 2316 46-47 -2.64629934332514 2316 48-49 -2.368543444774236 2316 50-51 -2.5016943203311577 2316 52-53 -2.5422817373892173 2316 54-55 -2.707332151249478 2316 56-57 -2.5075583093441622 2316 58-59 -2.677744879158702 2316 60-61 -2.5210382157764357 2316 62-63 -2.569121398267008 2316 64-65 -2.560412226466884 2316 66-67 -2.4852930899168193 2316 68-69 -2.4353003332856566 2316 70-71 -2.425315375523031 2316 72-73 -2.4203515827003805 2316 74-75 -2.57316853490606 2316 76-77 -3.001374828674397 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42508.0 3 4153.0 4 5452.0 5 6377.0 6 7415.0 7 8095.0 8 9204.0 9 9158.0 10 10660.0 11 11757.0 12 9688.0 13 8435.0 14 8436.0 15 8559.0 16 8931.0 17 9522.0 18 10040.0 19 10737.0 20 11524.0 21 12366.0 22 13737.0 23 15534.0 24 18210.0 25 21434.0 26 25126.0 27 29546.0 28 34769.0 29 41638.0 30 48931.0 31 58058.0 32 70945.0 33 88170.0 34 117190.0 35 156409.0 36 222271.0 37 305521.0 38 282243.0 39 76286.0 40 629.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.550912076747412 26.77989098675631 28.70678459854979 21.96241233794649 2 22.147486095474388 26.88673988548031 28.78697522001795 22.178798799027348 3 22.286226548131815 27.082962979876147 28.51082845554046 22.119982016451576 4 22.10339175182065 27.164786890771836 28.711286538738605 22.020534818668906 5 22.38222478342299 27.20169184224332 28.637420049309153 21.77866332502453 6 22.575154288100137 27.08055165335085 28.52584733415388 21.818446724395134 7 22.411160047560358 26.82566149834697 28.352833913339897 22.410344540752778 8 22.137450647575573 27.34056353373689 28.34207387287272 22.17991194581482 9 22.29701921712519 26.76930805409958 28.443052406041485 22.49062032273375 10-11 22.638260623722324 27.13162215649602 28.112168892175198 22.117948327606456 12-13 22.625501958903797 26.78217837931293 28.269950317893716 22.32236934388956 14-15 22.352371969633136 27.063014234807735 28.431289939413052 22.15332385614608 16-17 22.74032556305165 26.936239621451207 28.254930617598912 22.068504197898235 18-19 22.553580369603026 26.92316056089023 28.084252049408704 22.439007020098042 20-21 22.590015939529817 27.07151368994679 27.925735490787474 22.412734879735922 22-23 22.632077400161794 27.025469507050214 27.656063795548057 22.686389297239934 24-25 22.614097675325194 27.120943277586772 27.724668770607963 22.540290276480068 26-27 22.54965333861041 27.21603150587446 27.672864510930996 22.561450644584138 28-29 22.827027065227377 27.177974242179616 27.47400271484013 22.520995977752882 30-31 22.60549561991596 27.06165930831394 27.61302611904902 22.719818952721077 32-33 22.509514860360827 27.378470457316723 27.55813972452162 22.553874957800826 34-35 22.59194838511864 27.120835653975234 27.569660709242527 22.71755525166359 36-37 22.53510751881498 26.981226338576086 27.91950168850965 22.564164454099284 38-39 22.456408940462158 27.13187355656943 27.761600723726847 22.650116779241568 40-41 22.405043265814705 27.046013385573787 27.62504736488165 22.923895983729857 42-43 22.328333086872938 27.19305351380033 27.493318386235334 22.9852950130914 44-45 22.436107762212004 27.041867204181962 27.691778652795655 22.83024638081038 46-47 22.28709069389783 26.944844492359582 27.671046987462507 23.097017826280076 48-49 22.405484587712685 26.910627272129258 27.69182927777346 22.992058862384603 50-51 22.186095079691615 27.05360829955249 27.618315410071915 23.14198121068398 52-53 22.335629573925843 26.938713811645197 27.646698671573432 23.078957942855528 54-55 22.293844000752493 27.095759384171718 27.59716922554961 23.01322738952618 56-57 22.272009272641817 26.898299680410265 27.628188591921393 23.201502455026517 58-59 22.36350875056473 26.96185698666987 27.626514991298638 23.04811927146676 60-61 22.312664090690877 26.873359093091242 27.570063225036872 23.243913591181006 62-63 22.18759625561086 26.808599600310686 27.71606997363225 23.28773417044621 64-65 22.23998869131923 26.67693328150188 27.67715347456498 23.40592455261391 66-67 22.042901430228902 26.735972545868343 27.818947134920833 23.40217888898192 68-69 22.328517338099395 26.462191451375105 27.856694077503057 23.35259713302244 70-71 21.997291989995755 26.476467806416714 27.915019937512902 23.61122026607463 72-73 22.07982697890027 26.274478979823908 27.955217300369316 23.690476740906504 74-75 21.996263170908115 26.314153138182977 27.827979650629057 23.86160404027985 76-77 21.935909243476225 26.17951330541165 28.086421935800445 23.79815551531168 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5125.0 1 3042.0 2 921.0 3 906.5 4 930.0 5 963.5 6 1054.5 7 1133.0 8 1180.0 9 1206.0 10 1258.5 11 1360.0 12 1529.5 13 1650.0 14 1676.0 15 1879.5 16 2176.0 17 2295.0 18 2596.5 19 3147.0 20 3806.0 21 4757.5 22 5299.0 23 6222.5 24 8535.0 25 11755.5 26 13587.0 27 16086.0 28 21740.5 29 26965.0 30 29034.0 31 32143.0 32 39142.5 33 46435.5 34 53083.0 35 56328.0 36 59654.0 37 65612.0 38 68996.5 39 69749.0 40 72839.5 41 77400.0 42 81824.0 43 84778.0 44 85377.0 45 86845.5 46 88911.5 47 91220.5 48 92333.0 49 91264.5 50 88984.0 51 85142.0 52 82512.0 53 79874.0 54 74902.0 55 67137.0 56 57204.0 57 52702.0 58 49269.5 59 41426.5 60 33800.5 61 30585.0 62 26077.0 63 18485.0 64 13464.5 65 11528.0 66 9846.5 67 7194.0 68 5360.0 69 3755.0 70 3013.0 71 2626.0 72 1940.0 73 1436.5 74 1232.0 75 1078.5 76 805.5 77 579.0 78 472.0 79 361.0 80 217.5 81 147.0 82 100.5 83 92.0 84 81.0 85 66.0 86 47.5 87 33.0 88 34.0 89 32.5 90 28.5 91 27.0 92 23.0 93 17.0 94 17.5 95 18.0 96 16.0 97 13.0 98 12.0 99 32.5 100 51.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003859400412249194 2 0.008425451604205986 3 0.01114333921846598 4 0.01897085554753477 5 0.013535080319014776 6 0.013480722566729577 7 0.01723140747440837 8 0.018590351281538367 9 0.01712269196983797 10-11 0.020873376877516763 12-13 0.020112368345523966 14-15 0.008642882613346784 16-17 0.006115247132084989 18-19 0.012556640777881177 20-21 0.01152384348446238 22-23 0.01565503265813757 24-25 0.02269436157907096 26-27 0.014187373346437174 28-29 0.007881874081353987 30-31 0.007854695205211386 32-33 0.009403891145339583 34-35 0.010572582819471382 36-37 0.009349533393054383 38-39 0.016089894676419175 40-41 0.01201306325502918 42-43 0.01565503265813757 44-45 0.018291383643969768 46-47 0.01636168343784517 48-49 0.013861226832725976 50-51 0.026118899973038553 52-53 0.031038276554849146 54-55 0.024460988528339955 56-57 0.014921203002287374 58-59 0.016144252428704372 60-61 0.019623148574957165 62-63 0.02639068873446455 64-65 0.020058010593238765 66-67 0.020547230363805565 68-69 0.02986958488071735 70-71 0.0567766722618913 72-73 0.06933331303977248 74-75 0.08015050574452726 76-77 0.07756851251098026 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1839664.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.637907682222675 #Duplication Level Percentage of deduplicated Percentage of total 1 71.60249450283989 38.40607989960183 2 14.040735233832958 15.062313205257269 3 5.754525254247545 9.259820830270463 4 2.947171230354122 6.323203935097481 5 1.6728053682550967 4.486288995639669 6 1.0480118940514407 3.372789913380151 7 0.6844942569135638 2.570038783293894 8 0.46994166325742043 2.0165350039865357 9 0.32050029998912566 1.5471868952647254 >10 1.3772577751524926 12.205816514593103 >50 0.05831954801825369 2.1327336429122856 >100 0.023437363024393316 2.20891521771745 >500 1.0187002121839263E-4 0.03251136402250434 >1k 2.0374004243678526E-4 0.37576579896262585 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4831 0.2626023012898008 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2046 0.111215961175519 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.435775228519991E-5 0.0 2 0.0 0.0 0.0 4.892197705667991E-4 0.0 3 0.0 0.0 0.0 9.240817888483984E-4 0.0 4 0.0 0.0 0.0 0.0020655945868375963 0.0 5 0.0 0.0 0.0 0.0033701806416823942 0.0 6 0.0 0.0 0.0 0.006522930274223989 0.0 7 0.0 0.0 0.0 0.014350446603292774 0.0 8 0.0 0.0 0.0 0.02331947573035076 0.0 9 0.0 0.0 0.0 0.03315822889397194 0.0 10 0.0 0.0 0.0 0.04555179641499752 0.0 11 0.0 0.0 0.0 0.060989398063994296 0.0 12 0.0 0.0 0.0 0.08327607650092625 0.0 13 0.0 0.0 0.0 0.11056366814809661 0.0 14 0.0 0.0 0.0 0.13882969933640055 0.0 15 0.0 0.0 0.0 0.1717704972212317 0.0 16 0.0 0.0 0.0 0.20248262726236965 0.0 17 0.0 0.0 0.0 0.2353147096426304 0.0 18 0.0 0.0 0.0 0.27184311917828474 0.0 19 0.0 0.0 0.0 0.31125248958505464 0.0 20 0.0 0.0 0.0 0.3568586437523374 0.0 21 0.0 0.0 0.0 0.4044216770018873 0.0 22 0.0 0.0 0.0 0.45818149401195 0.0 23 0.0 0.0 0.0 0.5124305307925795 5.435775228519991E-5 24 0.0 0.0 0.0 0.5678210803711983 5.435775228519991E-5 25 0.0 0.0 0.0 0.6194609450421381 5.435775228519991E-5 26 0.0 0.0 0.0 0.6757755764096053 5.435775228519991E-5 27 0.0 0.0 0.0 0.7301333286948052 5.435775228519991E-5 28 0.0 0.0 0.0 0.783947503457153 5.435775228519991E-5 29 0.0 0.0 0.0 0.8447194705120066 5.435775228519991E-5 30 0.0 0.0 0.0 0.9076113899059828 5.435775228519991E-5 31 0.0 5.435775228519991E-5 0.0 0.9822445837935623 5.435775228519991E-5 32 0.0 5.435775228519991E-5 0.0 1.059650023047687 5.435775228519991E-5 33 0.0 5.435775228519991E-5 0.0 1.1460244914288695 1.0871550457039982E-4 34 0.0 5.435775228519991E-5 0.0 1.2297897877003627 1.0871550457039982E-4 35 0.0 5.435775228519991E-5 0.0 1.3128484331921482 1.0871550457039982E-4 36 0.0 5.435775228519991E-5 0.0 1.405528400838414 1.0871550457039982E-4 37 0.0 5.435775228519991E-5 0.0 1.500165247566947 1.0871550457039982E-4 38 0.0 5.435775228519991E-5 0.0 1.5926821419563573 2.1743100914079963E-4 39 0.0 5.435775228519991E-5 0.0 1.6905804538220022 2.1743100914079963E-4 40 0.0 5.435775228519991E-5 0.0 1.7947842649527306 2.1743100914079963E-4 41 0.0 5.435775228519991E-5 0.0 1.9136103114481775 2.1743100914079963E-4 42 0.0 5.435775228519991E-5 0.0 2.0438514859235166 2.1743100914079963E-4 43 0.0 5.435775228519991E-5 0.0 2.1844206333330436 2.1743100914079963E-4 44 0.0 5.435775228519991E-5 0.0 2.328686107897964 2.1743100914079963E-4 45 0.0 5.435775228519991E-5 0.0 2.4805616677828124 2.1743100914079963E-4 46 0.0 5.435775228519991E-5 0.0 2.6426564850972785 2.1743100914079963E-4 47 0.0 5.435775228519991E-5 0.0 2.806436392732586 2.1743100914079963E-4 48 0.0 5.435775228519991E-5 0.0 2.9685855677993374 2.1743100914079963E-4 49 0.0 5.435775228519991E-5 0.0 3.140356065020569 2.1743100914079963E-4 50 0.0 5.435775228519991E-5 0.0 3.323650405726263 2.1743100914079963E-4 51 0.0 5.435775228519991E-5 0.0 3.5214582662921057 2.1743100914079963E-4 52 0.0 5.435775228519991E-5 0.0 3.7316053366266884 2.1743100914079963E-4 53 0.0 5.435775228519991E-5 0.0 3.96056018925195 2.1743100914079963E-4 54 0.0 5.435775228519991E-5 0.0 4.196092329903721 2.1743100914079963E-4 55 0.0 5.435775228519991E-5 0.0 4.435646944224597 2.1743100914079963E-4 56 0.0 5.435775228519991E-5 0.0 4.695096495881857 2.1743100914079963E-4 57 0.0 5.435775228519991E-5 0.0 4.9509584358882925 2.1743100914079963E-4 58 0.0 5.435775228519991E-5 0.0 5.216006836030927 2.7178876142599953E-4 59 0.0 5.435775228519991E-5 0.0 5.484805921081241 2.7178876142599953E-4 60 0.0 5.435775228519991E-5 0.0 5.76453091434088 3.261465137111994E-4 61 0.0 5.435775228519991E-5 0.0 6.056214613103262 3.261465137111994E-4 62 0.0 5.435775228519991E-5 0.0 6.366434305394899 3.261465137111994E-4 63 0.0 5.435775228519991E-5 0.0 6.6982340253437584 3.805042659963993E-4 64 0.0 5.435775228519991E-5 0.0 7.036176171300847 3.805042659963993E-4 65 0.0 5.435775228519991E-5 0.0 7.397111646474573 3.805042659963993E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCCTA 75 0.001690518 23.661102 29 GTTACCG 125 0.0016033836 17.040163 42 CCGTATG 130 0.0020815874 16.387892 52 GCGGATT 175 9.156593E-4 14.198205 10 GCGTAAG 155 0.0067164754 13.7416935 38 TGCGAAA 190 0.0016996353 13.080852 50 AGTCGTG 380 1.2765668E-8 13.077294 11 GACGCGA 280 1.4576268E-5 12.684557 69 GGCGGAT 255 8.610502E-5 12.527828 9 ACCGTTC 260 1.0307905E-4 12.288913 39 GTCGTGT 430 7.140261E-8 11.557622 12 CGGATTT 215 0.0043024826 11.55668 11 ACGCGAC 310 4.130239E-5 11.458265 70 CGTGTGA 440 9.8265446E-8 11.294334 14 TCGCGTC 320 5.74003E-5 11.094158 58 TCGAACT 640 1.2732926E-11 11.094158 24 CGAACTC 645 1.6370905E-11 11.007258 25 CCGGGGT 460 1.8107312E-7 10.805627 48 GCCTCGA 700 7.2759576E-12 10.650392 21 CACGACT 335 9.164826E-5 10.594811 8 >>END_MODULE