FastQCFastQC Report
Thu 26 May 2016
SRR936118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936118_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1839664
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45010.2446642430356848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36140.19644891675871246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23720.12893658842049416No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA7550.042.571613100-101
GGTATCA52650.035.155081
CGAGTAC5450.034.402294100-101
CCTGTCG5800.033.8647296-97
TCGGTAT2550.033.84552100-101
CGAGCGT2000.032.73589396-97
CCGGTAT3200.032.55078100-101
CGGTATC7700.032.466232100-101
ACGTATC3500.031.461325100-101
CGTATAG1550.030.720736100-101
CGTACAT10100.030.644697100-101
TTATACG6600.029.75990396-97
AGTATCA17350.029.331884100-101
TCGAGTA2550.029.177172100-101
TATACGT3600.028.9332496-97
GTATCAA93550.028.691871
AACGTAT2650.028.07614598-99
TCGTATC2900.027.708252100-101
ATACACG7400.026.54261896-97
CCGTATC3500.026.35949100-101
Produced by FastQC (version 0.11.5)