FastQCFastQC Report
Thu 26 May 2016
SRR936117_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936117_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3046493
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57030.18719885455177476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC4600.032.4063344
CGCGGAA4100.024.2420759
GGGCGGT7150.021.3462831
CGAATCG1003.5811984E-421.29559316
CTCGCGC4950.020.79606636
ATTCGCG4800.020.70676856
TCGCGCT5200.019.7972337
CGCGCTT5050.019.6816838
ATACGTA2353.1468517E-1019.63521
AATTCGC5250.018.93159155
CGATCGT4900.018.8337651
GCGGTTA7950.018.3043883
TCGCGGA5650.018.21926158
AACTCGA5650.017.59101113
CGCTCCG5650.017.59014525
CTAGTGG8700.017.54407519
CAATTCG5700.017.43956854
GATCGTT6100.016.8738352
CCTCGCG6200.016.60276635
GACGTAT5900.016.24453539
Produced by FastQC (version 0.11.5)