##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936117_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3046493 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.55205149002476 39.0 36.0 41.0 30.0 41.0 2 36.41671095256086 39.0 36.0 41.0 30.0 41.0 3 36.32959045039657 39.0 36.0 41.0 29.0 41.0 4 36.20342111404818 39.0 36.0 41.0 29.0 41.0 5 36.13365006911226 39.0 36.0 41.0 29.0 41.0 6 36.043819893891104 39.0 35.0 41.0 29.0 41.0 7 35.88028431379951 39.0 35.0 40.0 28.0 41.0 8 35.70070339895742 39.0 35.0 40.0 27.0 41.0 9 35.61177360328745 38.0 35.0 40.0 27.0 41.0 10-11 35.8272820584193 39.0 35.0 40.0 28.0 41.0 12-13 36.0154656518167 39.0 35.0 41.0 29.0 41.0 14-15 35.96559929729036 39.0 35.0 41.0 28.0 41.0 16-17 35.80596656549022 39.0 35.0 41.0 27.5 41.0 18-19 35.65418220228965 39.0 35.0 41.0 27.0 41.0 20-21 35.46059600333892 39.0 35.0 41.0 26.5 41.0 22-23 35.20980648896945 39.0 35.0 41.0 25.5 41.0 24-25 34.98606676595023 39.0 34.5 41.0 24.5 41.0 26-27 34.0476996008197 38.0 33.0 40.0 23.5 40.5 28-29 34.075575588061426 38.0 33.5 39.5 23.0 40.5 30-31 34.44434961774079 38.0 34.0 40.0 23.5 41.0 32-33 34.2898966122686 38.0 34.0 40.0 23.0 41.0 34-35 34.01992701115676 38.0 33.0 40.0 22.0 41.0 36-37 33.730485348234836 37.5 33.0 40.0 21.5 41.0 38-39 33.454557420614464 37.0 33.0 40.0 20.5 41.0 40-41 33.07788414416183 36.5 32.5 40.0 19.5 41.0 42-43 32.716259318501635 36.0 32.0 39.0 18.0 41.0 44-45 32.353414401411726 35.5 32.0 39.0 16.0 41.0 46-47 31.96058451471906 35.0 31.0 38.5 13.0 40.0 48-49 31.504567382889114 35.0 31.0 37.5 8.0 40.0 50-51 31.06989036245939 35.0 31.0 37.0 7.0 39.0 52-53 30.619853877885163 35.0 30.5 36.5 3.5 39.0 54-55 30.163323861239792 34.5 30.0 36.0 2.0 38.5 56-57 29.720558031809034 34.0 29.5 35.5 2.0 37.0 58-59 29.30157824094787 34.0 29.0 35.0 2.0 37.0 60-61 28.957748795089962 34.0 29.0 35.0 2.0 36.5 62-63 28.618599648842128 34.0 29.0 35.0 2.0 36.0 64-65 28.25488898218378 34.0 29.0 35.0 2.0 36.0 66-67 27.888136457231315 34.0 27.5 35.0 2.0 35.0 68-69 27.54264542869457 34.0 27.0 35.0 2.0 35.0 70-71 27.13511650937652 33.0 26.0 35.0 2.0 35.0 72-73 26.66007832612778 33.0 25.0 35.0 2.0 35.0 74-75 26.088859879211935 33.0 24.5 35.0 2.0 35.0 76-77 24.3873100972167 31.0 19.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8453397175610817 1101 2 0.7143333582197542 1101 3 0.8031492029264768 1101 4 0.861750784231802 1101 5 0.8202722278115573 1101 6 0.8957366367112556 1101 7 0.979610099285992 1101 8 0.8553081513867156 1101 9 0.8398047495197147 1101 10-11 0.8490352526242688 1101 12-13 0.9325266934177776 1101 14-15 1.0402729127145136 1101 16-17 1.0984280563079665 1101 18-19 1.1691616766072812 1101 20-21 1.0118061363267685 1101 22-23 0.8678208902034044 1101 24-25 1.0038954166103977 1101 26-27 0.813029595328949 1101 28-29 0.909796609277997 1101 30-31 0.8726521453999467 1101 32-33 0.9635878366688928 1101 34-35 0.88008130890573 1101 36-37 0.9132312046613791 1101 38-39 0.9579832150094134 1101 40-41 0.8128385969374392 1101 42-43 0.8342727838479291 1101 44-45 0.8339907536478179 1101 46-47 0.8122772643042033 1101 48-49 0.8282550071142651 1101 50-51 0.6907603170895023 1101 52-53 0.5921528752685603 1101 54-55 0.5931132078754615 1101 56-57 0.6545683336926125 1101 58-59 0.608564116690971 1101 60-61 0.43784595178943064 1101 62-63 0.4950637963080524 1101 64-65 0.4424493782768977 1101 66-67 0.381823161979316 1101 68-69 0.43167081072284574 1101 70-71 0.5226165926258552 1101 72-73 0.4146366632570704 1101 74-75 0.5095423262557226 1101 76-77 0.40439871770837854 1102 1 1.107723362905105 1102 2 1.2699661615642697 1102 3 1.207775750201499 1102 4 1.2404982234342228 1102 5 1.3179932650437962 1102 6 1.1531301221773944 1102 7 1.0190908823198797 1102 8 1.355666281260305 1102 9 1.2479775461197278 1102 10-11 1.1164392253748545 1102 12-13 1.2570416784178846 1102 14-15 1.1224396222332587 1102 16-17 1.2606003662218654 1102 18-19 1.3692458077111027 1102 20-21 1.1997111604345037 1102 22-23 1.2628947831239472 1102 24-25 1.1883183443327638 1102 26-27 0.9384595452007787 1102 28-29 1.0642945377326356 1102 30-31 1.20896795411128 1102 32-33 1.0726381328239043 1102 34-35 1.2364419383030238 1102 36-37 1.2151208560974922 1102 38-39 1.2484398111958868 1102 40-41 1.2102730050943364 1102 42-43 1.268226988287637 1102 44-45 1.3007754283429662 1102 46-47 1.1302244278179607 1102 48-49 1.1148640542184296 1102 50-51 1.2687728577687274 1102 52-53 1.066752942713638 1102 54-55 1.2337379536107598 1102 56-57 1.388876587253069 1102 58-59 1.2994365115496258 1102 60-61 1.2037091274012504 1102 62-63 1.210782405617529 1102 64-65 1.3026809021677046 1102 66-67 1.3610566363698346 1102 68-69 1.1901889441614593 1102 70-71 1.1278997029906925 1102 72-73 1.278099304715866 1102 74-75 1.2261881802483892 1102 76-77 1.3446615657584147 1103 1 0.4095247846697987 1103 2 0.28186921630941697 1103 3 0.34523977378918147 1103 4 0.41959575877479693 1103 5 0.43863381920783695 1103 6 0.294299707009408 1103 7 0.36088789135514787 1103 8 0.47422849890035224 1103 9 0.4030546077732282 1103 10-11 0.42888937001841754 1103 12-13 0.4138813721242869 1103 14-15 0.552432186513613 1103 16-17 0.5652178546507542 1103 18-19 0.646051294155491 1103 20-21 0.6018358871908731 1103 22-23 0.5565073818845931 1103 24-25 0.46226023011292483 1103 26-27 0.19195658407240046 1103 28-29 0.3282340523471632 1103 30-31 0.5078463115920471 1103 32-33 0.503389826768398 1103 34-35 0.5286513309230401 1103 36-37 0.5076003869608954 1103 38-39 0.4021910862508591 1103 40-41 0.5053384962466083 1103 42-43 0.5625531504847103 1103 44-45 0.4752448861798797 1103 46-47 0.2679855235326265 1103 48-49 0.4131878073702424 1103 50-51 0.22796255126604592 1103 52-53 0.2835953027515288 1103 54-55 0.36433998617198426 1103 56-57 0.22917200153186457 1103 58-59 0.23674359299645786 1103 60-61 0.34027929041397087 1103 62-63 0.15207588631405144 1103 64-65 0.0366394992542709 1103 66-67 0.03110045128018868 1103 68-69 0.11259064225058069 1103 70-71 0.13676974417370857 1103 72-73 0.17744356918840154 1103 74-75 -0.030093071973041674 1103 76-77 0.2437532789526493 1104 1 0.3407669853004123 1104 2 0.4138835804609826 1104 3 0.3503467807096641 1104 4 0.4492666421793956 1104 5 0.5152400221627857 1104 6 0.2983255189071201 1104 7 0.5030178345585199 1104 8 0.455001595475629 1104 9 0.3159881818769179 1104 10-11 0.36288347028169454 1104 12-13 0.505402399676008 1104 14-15 0.45330224746258807 1104 16-17 0.5258843632029766 1104 18-19 0.4825702223349779 1104 20-21 0.5104675801153604 1104 22-23 0.5490878901842535 1104 24-25 0.5435977757061821 1104 26-27 0.2924473901898139 1104 28-29 0.26544609089744853 1104 30-31 0.4314639683995196 1104 32-33 0.32895519784923266 1104 34-35 0.43890481638167955 1104 36-37 0.34045487860382906 1104 38-39 0.2943336085898096 1104 40-41 0.4153114547689256 1104 42-43 0.40371188671880276 1104 44-45 0.33384923475041006 1104 46-47 0.1757319139924114 1104 48-49 0.348091208157868 1104 50-51 0.2899014210636892 1104 52-53 0.28831205115000813 1104 54-55 0.32852004269748747 1104 56-57 0.3061030026527476 1104 58-59 0.3739536286552543 1104 60-61 0.1610655669922778 1104 62-63 0.08563203725488577 1104 64-65 0.02530331660424423 1104 66-67 -0.04309220636385902 1104 68-69 -0.012468131623212031 1104 70-71 0.07655586467303621 1104 72-73 0.03625229818032949 1104 74-75 0.04208900014521788 1104 76-77 0.1319990171292993 1105 1 0.208487757766477 1105 2 0.3541431002738875 1105 3 0.3100685938857666 1105 4 0.3782877508934561 1105 5 0.43289915964048475 1105 6 0.18094024485750992 1105 7 0.30352237581672625 1105 8 0.5030640709630845 1105 9 0.31826062448431713 1105 10-11 0.36405475268117016 1105 12-13 0.3858024733768488 1105 14-15 0.16852575106710788 1105 16-17 0.32387405669162206 1105 18-19 0.49678411146164336 1105 20-21 0.45101254560798765 1105 22-23 0.35955413505385536 1105 24-25 0.39637853166210135 1105 26-27 0.2636703308823982 1105 28-29 0.45843892537667585 1105 30-31 0.527175561145313 1105 32-33 0.6506732737543288 1105 34-35 0.6156925332950038 1105 36-37 0.57735738312671 1105 38-39 0.6535206312450796 1105 40-41 0.7178199190010446 1105 42-43 0.5702364954974257 1105 44-45 0.4532629745409835 1105 46-47 0.37480131997039834 1105 48-49 0.4410449434702919 1105 50-51 0.5454911269325287 1105 52-53 0.4089139993862929 1105 54-55 0.3670681457954892 1105 56-57 0.5004641870697597 1105 58-59 0.5401267801738321 1105 60-61 0.4131918411004101 1105 62-63 0.4244477169637939 1105 64-65 0.43697462342558424 1105 66-67 0.5287346798384611 1105 68-69 0.16398299877069533 1105 70-71 -0.0549841782417424 1105 72-73 0.007216753966837075 1105 74-75 -0.08869626200780445 1105 76-77 -0.052768000680288196 1106 1 0.22086681061097124 1106 2 0.17271749265862724 1106 3 -0.16033673810344595 1106 4 0.10643951395317686 1106 5 -0.24400389030876113 1106 6 0.09406951488153226 1106 7 0.13807044621057685 1106 8 0.1400347312057164 1106 9 -0.09548702649848195 1106 10-11 -0.15153222660848087 1106 12-13 -0.1464758572689675 1106 14-15 -0.06305316399566152 1106 16-17 0.010991645598025457 1106 18-19 0.004709449362657381 1106 20-21 -0.09983346164649731 1106 22-23 0.10990887967744101 1106 24-25 0.3152568309676269 1106 26-27 0.3049837272670004 1106 28-29 0.08625548936898753 1106 30-31 0.03640331429456012 1106 32-33 0.018964207432553337 1106 34-35 0.08483606964938417 1106 36-37 0.20881382554181727 1106 38-39 0.04639124519940907 1106 40-41 0.08123734155358875 1106 42-43 0.13282217279307673 1106 44-45 -0.06571372653019125 1106 46-47 0.22374660613347075 1106 48-49 -0.011244702897158732 1106 50-51 -0.1936394857652921 1106 52-53 -0.06782299758342702 1106 54-55 0.0803478949383809 1106 56-57 -0.01814946492353897 1106 58-59 -0.015895549901987494 1106 60-61 -0.04618914728370527 1106 62-63 -0.17378639499035842 1106 64-65 -0.030851874619738595 1106 66-67 -0.08599249958131594 1106 68-69 -0.17264365246172275 1106 70-71 -0.2550370378986351 1106 72-73 -0.2376076453382865 1106 74-75 -0.3660614247498337 1106 76-77 -0.24091847113364295 1107 1 0.22912029932925293 1107 2 0.13701999710426804 1107 3 0.1757459502607972 1107 4 0.13807575254192272 1107 5 0.2048821316234566 1107 6 -0.01993763276455951 1107 7 -0.04015692832769702 1107 8 0.11250983157163574 1107 9 -0.02088240303079658 1107 10-11 0.055155334479429996 1107 12-13 0.1486280513187097 1107 14-15 0.15183122623304257 1107 16-17 0.16342171833230879 1107 18-19 0.06328558610264423 1107 20-21 -0.017149713251946253 1107 22-23 -0.05295855652860837 1107 24-25 -0.19756438902501117 1107 26-27 -0.1663115037505989 1107 28-29 -0.005870618002639105 1107 30-31 0.21565344613482296 1107 32-33 0.2195679031140756 1107 34-35 0.26581754969909355 1107 36-37 0.25737996896324233 1107 38-39 0.2211173232603798 1107 40-41 0.2690270295104824 1107 42-43 0.20987406304197265 1107 44-45 0.2722286307159436 1107 46-47 0.10991895741362256 1107 48-49 0.25051787154787064 1107 50-51 0.17672853607691508 1107 52-53 0.34084095573184925 1107 54-55 0.23851962538650184 1107 56-57 0.2506777279809782 1107 58-59 0.2769918844807968 1107 60-61 0.2995314390679127 1107 62-63 0.272415682373623 1107 64-65 0.2611174091962809 1107 66-67 0.27950089455816496 1107 68-69 0.11354958554658268 1107 70-71 0.3087828215717643 1107 72-73 0.4205927033892962 1107 74-75 0.5118853060156248 1107 76-77 0.5185402741163578 1108 1 -0.08769771949832261 1108 2 0.0677146041574801 1108 3 0.09139037780833092 1108 4 -0.056056291057863916 1108 5 -0.18475190144361164 1108 6 -0.13750866819478347 1108 7 -0.07169311675897916 1108 8 0.0674649227501547 1108 9 -0.09985647731288338 1108 10-11 -0.13386770964311268 1108 12-13 -0.1588487598309527 1108 14-15 -0.0313636076324002 1108 16-17 -0.1435786791291207 1108 18-19 -0.07221733221471283 1108 20-21 -0.20862081737388394 1108 22-23 -0.16204389841023925 1108 24-25 0.004607284071603601 1108 26-27 -0.24031633809126873 1108 28-29 -0.10938750363310845 1108 30-31 0.04349205519262256 1108 32-33 0.08603349893900258 1108 34-35 0.0826623539709459 1108 36-37 0.07464102767953307 1108 38-39 -0.007293716662893246 1108 40-41 0.06864825308686306 1108 42-43 0.02414271240754573 1108 44-45 -0.028441221402481176 1108 46-47 -0.08389090481415096 1108 48-49 -0.059491028132001134 1108 50-51 -0.05327645379214019 1108 52-53 -0.01302057648196353 1108 54-55 0.030283101273123947 1108 56-57 -0.14042594032881794 1108 58-59 -0.12278076326036924 1108 60-61 -0.15860852783910317 1108 62-63 -0.09247864472849798 1108 64-65 -0.16727613378355954 1108 66-67 -0.19927876924130672 1108 68-69 -0.1914347752101513 1108 70-71 -0.36364501973743657 1108 72-73 -0.3101762116706013 1108 74-75 -0.32768856780765176 1108 76-77 -0.2710389999981011 1109 1 0.08504240130004348 1109 2 -0.10604877443789462 1109 3 -0.21693107480008678 1109 4 0.057112997634398255 1109 5 0.06128895664854639 1109 6 0.03207945313957339 1109 7 -0.08874360661319258 1109 8 0.06410366010571522 1109 9 0.13212513624110755 1109 10-11 -0.11048923825625678 1109 12-13 0.001164483352447121 1109 14-15 0.17290928765204683 1109 16-17 0.20298850196532214 1109 18-19 0.0746096658679889 1109 20-21 0.025176023752734977 1109 22-23 0.019858828145430607 1109 24-25 0.13712248610628563 1109 26-27 0.11833024467580344 1109 28-29 0.15662558902000967 1109 30-31 0.2212265941893179 1109 32-33 0.17586619063342823 1109 34-35 0.3229086667406449 1109 36-37 0.4297351939354357 1109 38-39 0.2957720650655986 1109 40-41 0.28338354604008487 1109 42-43 0.3338235055715515 1109 44-45 0.34743656367944453 1109 46-47 0.18110594536167568 1109 48-49 0.2900204663334023 1109 50-51 0.18572314421372838 1109 52-53 0.2906546135625021 1109 54-55 0.32011804439573766 1109 56-57 0.2620488795678 1109 58-59 0.31533557880183594 1109 60-61 0.23510839955092777 1109 62-63 0.20562387987297726 1109 64-65 0.3568649953065872 1109 66-67 0.4246556091317579 1109 68-69 0.41231054627857233 1109 70-71 0.4895397831440427 1109 72-73 0.42716097309138235 1109 74-75 0.37338055604091025 1109 76-77 0.5342451454663468 1110 1 -0.5094455478468305 1110 2 -0.4477077105369247 1110 3 -0.5909155779784072 1110 4 -0.3057073703816613 1110 5 -0.44430643980511775 1110 6 -0.4890895264522612 1110 7 -0.6336968618825125 1110 8 -0.49750319168418144 1110 9 -0.6439383498746523 1110 10-11 -0.5247404794740547 1110 12-13 -0.5424669652925829 1110 14-15 -0.6042261824048296 1110 16-17 -0.5535228663852934 1110 18-19 -0.4683255766880521 1110 20-21 -0.5492538992984564 1110 22-23 -0.6379929751332583 1110 24-25 -0.47441913985557704 1110 26-27 -0.5172422678701949 1110 28-29 -0.3361278937501453 1110 30-31 -0.38827319838344465 1110 32-33 -0.15316644904539345 1110 34-35 -0.07430763302515686 1110 36-37 -0.07275371874439429 1110 38-39 -0.03645902225456865 1110 40-41 0.03820284346397784 1110 42-43 0.031012933473867577 1110 44-45 0.03541188652989291 1110 46-47 -0.026552803383719237 1110 48-49 0.024675213740561475 1110 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-0.6485580676988505 2314 14-15 -0.6379176429532336 2314 16-17 -1.0085481377898873 2314 18-19 -0.6239341273315873 2314 20-21 -0.528449019611827 2314 22-23 -0.5308512179983893 2314 24-25 -0.5296159165763399 2314 26-27 -0.4837951945629797 2314 28-29 -0.5817287443377239 2314 30-31 -0.7456436292732036 2314 32-33 -0.8116340036061089 2314 34-35 -0.7825806268695885 2314 36-37 -0.8406860746481399 2314 38-39 -0.8582610591879742 2314 40-41 -0.6839622600614916 2314 42-43 -0.5704104576845026 2314 44-45 -0.5070200776558877 2314 46-47 -0.3909702628129317 2314 48-49 -0.47363162453588004 2314 50-51 -0.36427626604695007 2314 52-53 -0.2245794764407023 2314 54-55 -0.2835549925165175 2314 56-57 -0.21018301042690624 2314 58-59 -0.27996905712961606 2314 60-61 -0.3863800070164878 2314 62-63 -0.26895648315556997 2314 64-65 -0.30238217342981244 2314 66-67 -0.2724848385047274 2314 68-69 -0.22060359160772336 2314 70-71 -0.18746471740946902 2314 72-73 -0.0043181979710240626 2314 74-75 -0.039107932341128304 2314 76-77 0.005386953809303208 2315 1 -1.3870154094678426 2315 2 -1.3242192496501701 2315 3 -1.0754357926070384 2315 4 -1.2652974522794338 2315 5 -1.147625231107007 2315 6 -1.2397623533373263 2315 7 -1.3637165399395599 2315 8 -1.3227009550278694 2315 9 -1.124327663627831 2315 10-11 -1.2130595305895682 2315 12-13 -1.3657729240612966 2315 14-15 -1.55984875230034 2315 16-17 -1.3127724794889417 2315 18-19 -1.23556213133363 2315 20-21 -1.383896723359456 2315 22-23 -1.3651486486718056 2315 24-25 -1.3816768410699254 2315 26-27 -1.320467752196663 2315 28-29 -1.3580756373254061 2315 30-31 -1.5570496118259811 2315 32-33 -1.5503105778225716 2315 34-35 -1.3950218719407204 2315 36-37 -1.6218732217096559 2315 38-39 -1.4303659186115354 2315 40-41 -1.3477623415120235 2315 42-43 -1.4445546463058605 2315 44-45 -1.425838201015992 2315 46-47 -1.5383720634797378 2315 48-49 -1.3527203694280345 2315 50-51 -1.2387765439490899 2315 52-53 -1.2796966291424425 2315 54-55 -1.2550208745567382 2315 56-57 -1.4000844401097616 2315 58-59 -1.24418238072386 2315 60-61 -1.2924844432259768 2315 62-63 -1.336919715682935 2315 64-65 -1.4923641607564626 2315 66-67 -1.5456710588570246 2315 68-69 -1.5404175164883362 2315 70-71 -1.6528508085850753 2315 72-73 -2.866955720812811 2315 74-75 -3.612016195240191 2315 76-77 -3.2295215850164745 2316 1 -3.2537040760405205 2316 2 -3.1498821100272067 2316 3 -2.4592788529067278 2316 4 -2.5099513716559514 2316 5 -3.066514582525727 2316 6 -2.6918349613041244 2316 7 -3.0918703948547943 2316 8 -2.8663050776351184 2316 9 -2.4945819433757705 2316 10-11 -2.6892218557085315 2316 12-13 -2.560353425948847 2316 14-15 -2.7768130189769806 2316 16-17 -3.895395921498391 2316 18-19 -3.6005723304312838 2316 20-21 -3.2012847881165527 2316 22-23 -3.34158550778697 2316 24-25 -3.384807824149938 2316 26-27 -3.0677200506035547 2316 28-29 -3.0914142397202014 2316 30-31 -3.299810987488261 2316 32-33 -3.3100206828778234 2316 34-35 -3.365058980588074 2316 36-37 -3.2794515970495866 2316 38-39 -3.2029098919582992 2316 40-41 -3.146583037389803 2316 42-43 -3.348827525270959 2316 44-45 -3.108704787570133 2316 46-47 -2.9249624879966554 2316 48-49 -3.055091285144435 2316 50-51 -3.3763616200237543 2316 52-53 -4.2690530050381845 2316 54-55 -3.4824834238102405 2316 56-57 -3.5296663306364167 2316 58-59 -3.515307755987049 2316 60-61 -3.3465523910872754 2316 62-63 -3.420637155199767 2316 64-65 -3.329136538830845 2316 66-67 -3.3013619826092686 2316 68-69 -3.281837935501976 2316 70-71 -3.0525538188505728 2316 72-73 -2.935330475056979 2316 74-75 -3.04929887456964 2316 76-77 -3.2347478522205115 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76857.0 3 6623.0 4 8278.0 5 9705.0 6 11019.0 7 12037.0 8 13690.0 9 14552.0 10 17438.0 11 19052.0 12 15483.0 13 13733.0 14 13713.0 15 14874.0 16 14512.0 17 15429.0 18 16187.0 19 17378.0 20 18562.0 21 19879.0 22 22298.0 23 25424.0 24 29431.0 25 34356.0 26 40990.0 27 47812.0 28 57177.0 29 67353.0 30 80283.0 31 96597.0 32 118561.0 33 149081.0 34 198634.0 35 264995.0 36 375086.0 37 507389.0 38 460538.0 39 120685.0 40 802.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.875900672215224 26.75834819612783 28.29403351129293 22.071717620364016 2 22.50316553663766 26.93915177078002 28.153693574740313 22.403989117842006 3 22.655848355421902 27.11083461640737 27.74870570932382 22.48461131884691 4 22.317851313251225 26.925655312426542 28.32790768830753 22.428585686014703 5 22.655368752519607 26.989522367558866 28.012467056322365 22.342641823599163 6 22.754961617551665 26.8498225977157 28.103292512861994 22.291923271870644 7 22.60748522652659 26.552790544977018 28.236802363755746 22.602921864740644 8 22.60001930424608 27.012878033278152 27.895029550598466 22.492073111877296 9 22.417807468126615 26.796791026008894 28.132594080417395 22.6528074254471 10-11 22.956518570209365 26.93429318525165 27.698356455501543 22.410831789037445 12-13 23.071879889612536 26.544255774297948 27.712427913609922 22.671436422479594 14-15 22.69256772116755 26.78202562847978 27.884804298517572 22.640602351835096 16-17 22.907503230967656 26.618626984004536 28.001829103558713 22.4720406814691 18-19 22.89847136035189 26.585522679590085 27.68715178072082 22.828854179337206 20-21 22.774942165995903 26.84523797798007 27.66093875275545 22.718881103268583 22-23 22.948978139538777 26.81605395421922 27.286219588248432 22.948748317993566 24-25 22.939842276580514 26.923539713940194 27.263061527104536 22.873556482374756 26-27 23.02951136373337 26.903312887302995 27.16562991875215 22.90154583021149 28-29 23.176822980055913 26.906993421237285 27.148225116097986 22.767958482608815 30-31 22.866657628204727 26.79270944091423 27.274844636389574 23.065788294491472 32-33 22.868966119466585 27.12891647030012 27.137960602381874 22.864156807851423 34-35 22.96384240494517 26.745304318751355 27.28003313063308 23.01082014567039 36-37 23.014712303170093 26.874820675749582 27.498442300103935 22.61202472097639 38-39 22.838157635773634 26.870243743256644 27.5442305921416 22.74736802882812 40-41 22.73813672316069 26.762467895776727 27.400818119100823 23.09857726196176 42-43 22.6741688526977 27.194899091297227 27.089007698670294 23.041924357334782 44-45 22.752442151956416 26.854085442161146 27.482433183063893 22.911039222818545 46-47 22.75588640681134 26.794404392224436 27.379663307445863 23.07004589351837 48-49 22.76042069375695 26.79392148838138 27.318631344991108 23.127026472870565 50-51 22.669760758448714 27.044707809287132 27.182096517537747 23.1034349147264 52-53 22.761929937477014 26.842687700309988 27.341147612042242 23.054234750170757 54-55 22.74991783257941 26.769997875631525 27.345268322185596 23.134815969603473 56-57 22.688847739868148 27.189707929503847 27.15464371076616 22.966800619861843 58-59 22.8427543328434 27.041650648280896 27.15699944090225 22.958595577973455 60-61 22.748416056060027 26.85900420174413 27.13171702871881 23.260862713477035 62-63 22.758630766081296 26.809127066014792 27.313151033009447 23.11909113489446 64-65 22.73985007385003 26.82129343357741 27.23121568624876 23.207640806323802 66-67 22.706337108460833 26.9187522624908 27.395753043127076 22.979157585921296 68-69 22.7723924886505 26.639078792918053 27.450788188607007 23.13774052982444 70-71 22.57635071036347 26.593081450446327 27.465926085152315 23.36464175403789 72-73 22.72985916205546 26.426851099576755 27.475209873801397 23.36807986456639 74-75 22.54824385105164 26.444810466801155 27.362764750437236 23.64418093170996 76-77 22.410582118707147 26.340124463625774 27.76150281540513 23.487790602261953 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5386.0 1 3271.5 2 1133.5 3 1141.0 4 1172.0 5 1217.5 6 1361.0 7 1530.5 8 1666.0 9 1730.0 10 1803.0 11 1937.0 12 2079.0 13 2160.0 14 2312.5 15 2620.0 16 2955.0 17 3135.0 18 3491.5 19 4190.5 20 5059.5 21 6392.0 22 7198.0 23 8255.0 24 10927.5 25 14956.0 26 17369.0 27 20157.5 28 28186.0 29 37029.5 30 40633.0 31 47800.0 32 61027.0 33 73580.5 34 87101.0 35 94128.0 36 102191.5 37 115307.0 38 121798.5 39 123238.0 40 125991.5 41 132270.0 42 140390.0 43 144985.0 44 144036.0 45 142793.5 46 144643.5 47 147654.0 48 148521.0 49 147927.5 50 146486.0 51 140746.5 52 135855.0 53 133822.0 54 125142.5 55 110902.0 56 96499.5 57 89691.0 58 85672.0 59 73763.5 60 60389.0 61 54904.0 62 47460.5 63 33897.0 64 24184.0 65 20591.0 66 18071.0 67 13798.5 68 10212.0 69 7252.0 70 6126.0 71 5161.0 72 3542.0 73 2609.0 74 2330.0 75 1902.0 76 1247.0 77 798.0 78 576.0 79 486.0 80 340.0 81 228.0 82 150.0 83 128.0 84 111.5 85 87.5 86 78.0 87 76.0 88 69.5 89 59.5 90 52.0 91 48.0 92 47.5 93 45.5 94 41.0 95 31.5 96 25.0 97 31.0 98 42.0 99 91.0 100 135.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009716089943420188 2 0.013326799044015528 3 0.013884817723198445 4 0.015263452107062122 5 0.012637481852083693 6 0.010142810109854183 7 0.01618254169630457 8 0.017429877567419327 9 0.017561176080168245 10-11 0.019908136995555218 12-13 0.01624819095267903 14-15 0.00766455068171829 16-17 0.006302328611948229 18-19 0.018037133188883087 20-21 0.023207012128371868 22-23 0.021385245263980585 24-25 0.01941576757274676 26-27 0.024651295768610006 28-29 0.008255393989088436 30-31 0.008271806303182052 32-33 0.010471056391726487 34-35 0.01212870011518162 36-37 0.010996250442722172 38-39 0.014426424088287745 40-41 0.011800453833309316 42-43 0.014689021113785589 44-45 0.013425272928577219 46-47 0.024519997255861084 48-49 0.0234531968397761 50-51 0.03673075894151078 52-53 0.039816273991110436 54-55 0.02895132206113718 56-57 0.02143448220626143 58-59 0.016871858888236408 60-61 0.020187146335146674 62-63 0.026407413376626828 64-65 0.026046342466567295 66-67 0.029558577682600944 68-69 0.04314797375211432 70-71 0.055096138412266175 72-73 0.08665701841428818 74-75 0.08729709866393916 76-77 0.09166277421284079 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3046493.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.025666134413115 #Duplication Level Percentage of deduplicated Percentage of total 1 74.29288680476415 37.908440382616405 2 13.344150409807904 13.617883273165003 3 5.029423017687494 7.698889792477637 4 2.3706802805025182 4.838621620174333 5 1.3533847951426734 3.452868035417057 6 0.8224111782950599 2.5178446925335813 7 0.544187931182391 1.9437286183622764 8 0.3817207640819122 1.558204501169338 9 0.27417541209913837 1.259098473603219 >10 1.399434586564271 12.946607781551936 >50 0.11687709933154927 4.169698326093059 >100 0.06647170323370614 6.226195212469332 >500 0.003292259733448845 1.134029602869915 >1k 8.392034613360855E-4 0.5400376245140539 >5k 6.455411241046812E-5 0.18785206298283927 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5703 0.18719885455177476 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.2824628187230366E-5 0.0 2 0.0 0.0 0.0 1.969477691233822E-4 0.0 3 0.0 0.0 0.0 2.625970254978429E-4 0.0 4 0.0 0.0 0.0 8.206157046807592E-4 0.0 5 0.0 0.0 0.0 0.0017725299221104398 0.0 6 0.0 0.0 0.0 0.0032824628187230367 0.0 7 0.0 0.0 0.0 0.006597750265633304 0.0 8 0.0 0.0 0.0 0.010274108622603105 0.0 9 0.0 0.0 0.0 0.014606959543317513 0.0 10 0.0 0.0 0.0 0.018611564182159618 0.0 11 0.0 0.0 0.0 0.02412610171761432 0.0 12 0.0 0.0 0.0 0.031610116944302845 0.0 13 0.0 0.0 0.0 0.042376594989714404 0.0 14 0.0 0.0 0.0 0.055506446264606546 0.0 15 0.0 0.0 0.0 0.06985080878242622 0.0 16 0.0 0.0 0.0 0.08537685791498618 0.0 17 0.0 0.0 0.0 0.09876930621537618 0.0 18 0.0 0.0 0.0 0.1146892508861829 0.0 19 0.0 0.0 0.0 0.12995270299324502 0.0 20 0.0 0.0 0.0 0.14931923362371094 0.0 21 0.0 0.0 0.0 0.16878423813873855 0.0 22 0.0 0.0 0.0 0.19182712712617425 0.0 23 0.0 0.0 0.0 0.21427917280623984 0.0 24 0.0 0.0 0.0 0.23866787154935198 0.0 25 0.0 0.0 0.0 0.2633519919461492 0.0 26 0.0 0.0 0.0 0.28895520193218893 0.0 27 0.0 0.0 0.0 0.3154446768792838 0.0 28 0.0 0.0 0.0 0.3422295734800638 0.0 29 0.0 0.0 0.0 0.36789843272247796 0.0 30 0.0 0.0 0.0 0.39891770635941065 0.0 31 0.0 0.0 0.0 0.4301995770218412 0.0 32 0.0 0.0 0.0 0.46656926505329244 0.0 33 0.0 0.0 0.0 0.5070748562363347 0.0 34 0.0 0.0 0.0 0.5468254809710706 0.0 35 0.0 0.0 0.0 0.5906463596010232 0.0 36 0.0 0.0 0.0 0.6349596076537842 0.0 37 0.0 0.0 0.0 0.6806843147185961 0.0 38 0.0 0.0 0.0 0.7283784994746418 0.0 39 0.0 0.0 0.0 0.7773200201018023 0.0 40 0.0 0.0 0.0 0.8292485818940007 0.0 41 0.0 0.0 0.0 0.8878405432082069 0.0 42 0.0 0.0 0.0 0.9478439635344641 0.0 43 0.0 0.0 0.0 1.0128695519733675 0.0 44 0.0 0.0 0.0 1.0834753272041 0.0 45 0.0 0.0 0.0 1.1577574607918022 0.0 46 0.0 0.0 0.0 1.2387029939015124 0.0 47 0.0 0.0 0.0 1.3204363180877159 0.0 48 0.0 0.0 0.0 1.4043032431060896 0.0 49 0.0 0.0 0.0 1.490139645815697 0.0 50 0.0 0.0 0.0 1.5784707202675339 0.0 51 0.0 0.0 0.0 1.6753033734198635 0.0 52 3.2824628187230366E-5 0.0 0.0 1.7754513140191033 0.0 53 3.2824628187230366E-5 0.0 0.0 1.8823283033967253 0.0 54 3.2824628187230366E-5 0.0 0.0 1.9983305394103974 0.0 55 3.2824628187230366E-5 0.0 0.0 2.1216198428816346 0.0 56 3.2824628187230366E-5 0.0 0.0 2.2538702698479858 0.0 57 3.2824628187230366E-5 0.0 0.0 2.3906176708759874 0.0 58 3.2824628187230366E-5 0.0 0.0 2.53458649010518 0.0 59 3.2824628187230366E-5 0.0 0.0 2.678489660077998 0.0 60 3.2824628187230366E-5 0.0 0.0 2.821834811371633 0.0 61 3.2824628187230366E-5 0.0 0.0 2.9753884220314966 0.0 62 3.2824628187230366E-5 0.0 0.0 3.1358023799824912 0.0 63 3.2824628187230366E-5 0.0 0.0 3.3056698308514085 0.0 64 3.2824628187230366E-5 0.0 0.0 3.4830541215752016 0.0 65 3.2824628187230366E-5 0.0 0.0 3.675504916636933 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAC 460 0.0 32.406334 4 CGCGGAA 410 0.0 24.24207 59 GGGCGGT 715 0.0 21.346283 1 CGAATCG 100 3.5811984E-4 21.295593 16 CTCGCGC 495 0.0 20.796066 36 ATTCGCG 480 0.0 20.706768 56 TCGCGCT 520 0.0 19.79723 37 CGCGCTT 505 0.0 19.68168 38 ATACGTA 235 3.1468517E-10 19.6352 1 AATTCGC 525 0.0 18.931591 55 CGATCGT 490 0.0 18.833765 1 GCGGTTA 795 0.0 18.304388 3 TCGCGGA 565 0.0 18.219261 58 AACTCGA 565 0.0 17.591011 13 CGCTCCG 565 0.0 17.590145 25 CTAGTGG 870 0.0 17.544075 19 CAATTCG 570 0.0 17.439568 54 GATCGTT 610 0.0 16.873835 2 CCTCGCG 620 0.0 16.602766 35 GACGTAT 590 0.0 16.244535 39 >>END_MODULE