FastQCFastQC Report
Thu 26 May 2016
SRR936117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936117_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3046493
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68780.22576779267177047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49050.16100480125836494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33680.11055334773459188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13600.047.052372100-101
GGTATCA87900.038.110941
CGGTATC10700.037.273743100-101
CGTACAT18300.035.943733100-101
ACGTATC5300.035.37909100-101
GCGTATC4150.033.707905100-101
TACGCAG10350.031.920126100-101
TCGTACA9650.030.842861100-101
GTATCAA168500.030.7926251
CCTGTCG7200.028.930996-97
TCGTATC5250.028.912981100-101
TCGAGTA4650.028.803253100-101
CGAGTAC12350.028.678867100-101
CCGTACG1303.6379788E-1127.4684696-97
CCGTACA9100.027.146801100-101
GGGTATC16500.026.877216100-101
CCGGTAT5550.026.277563100-101
CCGTATC6550.025.90094100-101
TATACGT4600.025.87608596-97
TCGGTAT3950.025.619095100-101
Produced by FastQC (version 0.11.5)