FastQCFastQC Report
Thu 26 May 2016
SRR936116_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936116_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059710
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58280.1904755679459818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC3950.032.3500944
GGGCGGT6300.021.4087811
ATTCGCG5350.019.9061556
CGCGGAA5100.019.49172659
CGATCGT5700.018.6807921
TCGCGCT5500.018.07119237
AATTCGC5950.017.8988155
GACGAAT4750.017.19110547
CTCGCGC5850.016.988936
GACGTAT5050.016.87123339
CCTCGCG6500.016.3813535
ACTCGAG6550.016.25683214
GCGGTTA7900.016.1745193
GCCTAGT8150.016.11387817
CAATTCG6200.016.03246954
CGCGCTT6500.015.83815138
GCGTAAG6600.015.5981838
CGCTCCG6900.015.43347125
CGGTTCA5800.015.30042410
TCGCGGA6500.015.29325958
Produced by FastQC (version 0.11.5)