##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936116_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3059710 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.541608845282724 39.0 36.0 41.0 30.0 41.0 2 36.40882959496162 39.0 36.0 41.0 30.0 41.0 3 36.276362792552234 39.0 36.0 41.0 29.0 41.0 4 36.16499733634887 39.0 36.0 41.0 29.0 41.0 5 36.09551166613829 39.0 35.0 41.0 29.0 41.0 6 36.00697517084952 39.0 35.0 40.0 28.0 41.0 7 35.864958443774086 39.0 35.0 40.0 28.0 41.0 8 35.7474120096349 39.0 35.0 40.0 27.0 41.0 9 35.9124325507973 39.0 35.0 40.0 29.0 41.0 10-11 36.135455974585824 39.0 35.0 41.0 29.0 41.0 12-13 36.0610500668364 39.0 35.0 41.0 28.5 41.0 14-15 35.91473440293361 39.0 35.0 41.0 27.5 41.0 16-17 35.77150694020021 39.0 35.0 41.0 27.0 41.0 18-19 35.621883283056235 39.0 35.0 41.0 27.0 41.0 20-21 35.428260194593605 39.0 35.0 41.0 26.5 41.0 22-23 35.16023528373604 39.0 35.0 41.0 25.5 41.0 24-25 34.942158080340946 39.0 34.0 41.0 24.0 41.0 26-27 33.98496409790471 38.0 33.0 40.0 23.0 40.5 28-29 33.98971781639436 38.0 33.0 39.5 23.0 40.5 30-31 34.39271940804848 38.0 34.0 40.0 23.0 41.0 32-33 34.27162999107759 38.0 34.0 40.0 23.0 41.0 34-35 34.016675763389344 38.0 33.0 40.0 22.0 41.0 36-37 33.71291968846721 37.5 33.0 40.0 21.5 41.0 38-39 33.40627853620114 37.0 33.0 40.0 20.0 41.0 40-41 33.04882995447281 36.5 32.0 40.0 18.5 41.0 42-43 32.68145755643509 36.0 32.0 39.0 18.0 41.0 44-45 32.329769814786374 35.5 32.0 39.0 15.5 40.5 46-47 31.91595755806923 35.0 31.0 38.5 12.0 40.0 48-49 31.499261041079055 35.0 31.0 37.5 8.0 40.0 50-51 31.052194489020202 35.0 31.0 37.0 7.0 39.0 52-53 30.568654872520597 35.0 30.0 36.5 2.0 39.0 54-55 30.109126681940445 34.5 30.0 36.0 2.0 38.5 56-57 29.673841638586662 34.0 29.5 35.5 2.0 37.0 58-59 29.257863326916603 34.0 29.0 35.0 2.0 37.0 60-61 28.906163165790225 34.0 29.0 35.0 2.0 36.5 62-63 28.547398609672157 34.0 29.0 35.0 2.0 36.0 64-65 28.18117877184439 34.0 28.5 35.0 2.0 36.0 66-67 27.840718074588768 34.0 27.5 35.0 2.0 35.0 68-69 27.485631808243266 34.0 27.0 35.0 2.0 35.0 70-71 27.047268531985058 33.0 26.0 35.0 2.0 35.0 72-73 26.584016622490367 33.0 25.0 35.0 2.0 35.0 74-75 26.02946455709855 33.0 24.5 35.0 2.0 35.0 76-77 24.27026826071752 31.0 12.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.17859206577711 1101 2 1.0839500569689946 1101 3 1.161241649359006 1101 4 1.022526446850975 1101 5 1.1047522035865143 1101 6 1.1382539549537967 1101 7 1.1855621687876123 1101 8 0.9972669794879891 1101 9 1.1043075757587388 1101 10-11 1.078128355246676 1101 12-13 1.231212429135219 1101 14-15 1.368087313326157 1101 16-17 1.2564145234644641 1101 18-19 1.274265994615611 1101 20-21 1.231157322005643 1101 22-23 1.1356230477763845 1101 24-25 1.2377601276085528 1101 26-27 1.2132701398631411 1101 28-29 1.2782323517313472 1101 30-31 1.2906609109080591 1101 32-33 1.3423431008855786 1101 34-35 1.2883661292448139 1101 36-37 1.358030405918612 1101 38-39 1.377710116437811 1101 40-41 1.4305254246209742 1101 42-43 1.2723766851985587 1101 44-45 1.1913418988908688 1101 46-47 1.2737552437797817 1101 48-49 1.2668181370892597 1101 50-51 1.2120927823540626 1101 52-53 1.334502197699468 1101 54-55 1.3950138682649005 1101 56-57 1.416102325439077 1101 58-59 1.5401727671896097 1101 60-61 1.3395348561760123 1101 62-63 1.4013849366604845 1101 64-65 1.3889412594895028 1101 66-67 1.5980485948894163 1101 68-69 1.3929843741967538 1101 70-71 1.4608671739140071 1101 72-73 1.4753907520200684 1101 74-75 1.6563965384297674 1101 76-77 1.8370966903967165 1102 1 1.0231007446378655 1102 2 1.1794526029605379 1102 3 1.1649771717375543 1102 4 0.9721793023717993 1102 5 1.1427605933675196 1102 6 1.0660380019309983 1102 7 0.9746991388536799 1102 8 1.1072592126464826 1102 9 1.2379404126311826 1102 10-11 1.1770115421494225 1102 12-13 1.1753029250288947 1102 14-15 1.2741740840217304 1102 16-17 1.2995523995791203 1102 18-19 1.2712887468339602 1102 20-21 1.3647279644393677 1102 22-23 1.2444110100178918 1102 24-25 1.3039434688530136 1102 26-27 1.018983793681926 1102 28-29 1.2407759997884469 1102 30-31 1.4351661441969057 1102 32-33 1.431481911907035 1102 34-35 1.4226041180060278 1102 36-37 1.424629392597069 1102 38-39 1.4495146532484569 1102 40-41 1.4428565915976108 1102 42-43 1.475567036005053 1102 44-45 1.4194184214356085 1102 46-47 1.433882476186966 1102 48-49 1.3591241999026593 1102 50-51 1.3217157671298594 1102 52-53 1.409648255119368 1102 54-55 1.2820965062644092 1102 56-57 1.2433188357240468 1102 58-59 1.3781426959297072 1102 60-61 1.204073378416247 1102 62-63 1.2422859027142614 1102 64-65 1.1944540874656546 1102 66-67 1.189166681728775 1102 68-69 1.2199261716458842 1102 70-71 1.2910853122006074 1102 72-73 1.391033884808678 1102 74-75 1.213197475426302 1102 76-77 1.3538656793539694 1103 1 0.6571643335246335 1103 2 0.5956853053021831 1103 3 0.5967110005500018 1103 4 0.7000105129948366 1103 5 0.5382757152028077 1103 6 0.5993915001119632 1103 7 0.5660714647608245 1103 8 0.5783453417519198 1103 9 0.5591295865085826 1103 10-11 0.618043766296104 1103 12-13 0.6994221832985801 1103 14-15 0.5618261155701134 1103 16-17 0.4725712333067449 1103 18-19 0.4321264522183341 1103 20-21 0.47575963645331854 1103 22-23 0.45966811782660955 1103 24-25 0.6852312704405179 1103 26-27 0.6455027888483613 1103 28-29 0.611870410884876 1103 30-31 0.8197504782708265 1103 32-33 0.7897804668796482 1103 34-35 0.7518680530560147 1103 36-37 0.7929765832617051 1103 38-39 0.6748034726806509 1103 40-41 0.699644583430846 1103 42-43 0.7618228919128072 1103 44-45 0.8397378662301378 1103 46-47 0.8083401558131484 1103 48-49 0.799259688474109 1103 50-51 0.8619382166067098 1103 52-53 0.8409476995057972 1103 54-55 0.826695807936531 1103 56-57 0.8578938195501564 1103 58-59 0.7832624667226042 1103 60-61 0.6584865993545606 1103 62-63 0.6142462019702002 1103 64-65 0.5307918630669128 1103 66-67 0.6719464419831418 1103 68-69 0.592265882112283 1103 70-71 0.45836034511851764 1103 72-73 0.3364816704563509 1103 74-75 0.5414627704032142 1103 76-77 0.589270890766791 1104 1 0.3394787863983453 1104 2 0.29534985022719695 1104 3 0.5519237712358134 1104 4 0.44127944082521253 1104 5 0.3722275584512502 1104 6 0.4071254472766199 1104 7 0.353665943086078 1104 8 0.21221226854832764 1104 9 0.1336831903113449 1104 10-11 0.32330648095577175 1104 12-13 0.37647848314021104 1104 14-15 0.4439672265954542 1104 16-17 0.4884554053947241 1104 18-19 0.2939726481718239 1104 20-21 0.3326404422940712 1104 22-23 0.2656576518154168 1104 24-25 0.36242921466701716 1104 26-27 0.12768975007921313 1104 28-29 0.30226923749390266 1104 30-31 0.45528064466495977 1104 32-33 0.48991766660173397 1104 34-35 0.5657783270109888 1104 36-37 0.5660722207082216 1104 38-39 0.5612297064839424 1104 40-41 0.5726415025777598 1104 42-43 0.800258464186534 1104 44-45 0.6193714856593928 1104 46-47 0.5426472211894549 1104 48-49 0.5628166117381781 1104 50-51 0.44671793678009664 1104 52-53 0.3364120381048856 1104 54-55 0.305084336963688 1104 56-57 0.3983055122882817 1104 58-59 0.28133266432295656 1104 60-61 0.3012750170312195 1104 62-63 0.12370643276516446 1104 64-65 0.03889682260878402 1104 66-67 0.12876361896727317 1104 68-69 -0.14727512458044245 1104 70-71 -0.09107670325110817 1104 72-73 -0.255049141583541 1104 74-75 -0.28276590515745426 1104 76-77 -0.30543224084903287 1105 1 0.008041508219477578 1105 2 0.22601202638182372 1105 3 0.1285332098002101 1105 4 0.24628551742500804 1105 5 0.080111857788431 1105 6 0.16076871129059356 1105 7 0.2874867580731717 1105 8 0.15477125011157256 1105 9 0.16330159544537537 1105 10-11 0.34144794768659636 1105 12-13 0.3497713853458393 1105 14-15 0.27448251812884195 1105 16-17 0.2954750654951752 1105 18-19 0.1950596104294462 1105 20-21 0.17380601604462242 1105 22-23 0.2292507084540958 1105 24-25 0.3679270060059636 1105 26-27 0.12196917684832442 1105 28-29 0.31027103076193896 1105 30-31 0.5430844717871253 1105 32-33 0.3701367224607992 1105 34-35 0.3560954826162259 1105 36-37 0.28325938480027446 1105 38-39 0.2223413376489063 1105 40-41 0.31102214207420786 1105 42-43 0.42036027390509645 1105 44-45 0.23691784253303894 1105 46-47 0.28829786935628476 1105 48-49 0.1967430014807583 1105 50-51 0.014355528689101504 1105 52-53 0.0640377727861079 1105 54-55 -0.06998538275558275 1105 56-57 0.0073363124010725755 1105 58-59 0.1070181086810571 1105 60-61 0.18997228901992003 1105 62-63 0.11757769374050042 1105 64-65 0.2371848665172358 1105 66-67 0.2812180169364922 1105 68-69 0.21978196104236858 1105 70-71 0.10630891339012649 1105 72-73 -0.3486536149338022 1105 74-75 -0.5664839215588842 1105 76-77 -0.5890881375828556 1106 1 0.15661086842278138 1106 2 0.025508774647114763 1106 3 0.09273463122512027 1106 4 0.10837730135854429 1106 5 0.1065995132696429 1106 6 0.08335942991001843 1106 7 -0.049161648025759064 1106 8 0.10780162173904984 1106 9 0.12427620580199061 1106 10-11 0.027720429838744565 1106 12-13 0.3058414692493301 1106 14-15 0.17025025541447292 1106 16-17 0.06479323016406369 1106 18-19 0.1521903781370071 1106 20-21 0.21549360484938518 1106 22-23 0.16203165161036281 1106 24-25 -0.054070347057745494 1106 26-27 -0.04746967311547934 1106 28-29 0.06679004887114104 1106 30-31 0.24198746577938124 1106 32-33 0.13581552122366958 1106 34-35 0.19142996701014425 1106 36-37 0.20391486281053517 1106 38-39 0.2078303486102797 1106 40-41 0.17187597567301083 1106 42-43 0.2783607508841186 1106 44-45 0.15796106606973837 1106 46-47 0.2255234520778373 1106 48-49 0.08625267574021933 1106 50-51 -0.024883998466005863 1106 52-53 0.007254404033766093 1106 54-55 -0.030032616483076424 1106 56-57 -0.010628144201842105 1106 58-59 -0.06407290422023948 1106 60-61 -0.22857399738060025 1106 62-63 -0.1258296605167999 1106 64-65 -0.14144512444165613 1106 66-67 -0.16654094768520267 1106 68-69 -0.10886704276660097 1106 70-71 -0.28373296061149844 1106 72-73 -0.2398517514687022 1106 74-75 -0.24269096886669672 1106 76-77 -0.29363079107703527 1107 1 8.865574264405041E-4 1107 2 -0.043242046029554615 1107 3 0.00978184870565002 1107 4 -0.01971148248372856 1107 5 0.10522320808977526 1107 6 -0.11143273828128031 1107 7 -0.1097482555550684 1107 8 -0.03132435152269153 1107 9 -0.18485263678856967 1107 10-11 -0.01883382236066211 1107 12-13 -0.03100496816282572 1107 14-15 -0.18419209782422286 1107 16-17 -0.015527915816683446 1107 18-19 0.017787855072960213 1107 20-21 -0.03305531720700117 1107 22-23 -0.023789000748564604 1107 24-25 0.04497620901818067 1107 26-27 -0.24635610605342606 1107 28-29 -0.0953598565382805 1107 30-31 0.05462029381904898 1107 32-33 0.15254395140900812 1107 34-35 0.13481239504591258 1107 36-37 0.03719226854822466 1107 38-39 0.20992337808655748 1107 40-41 0.22641024469933768 1107 42-43 0.1379210870332841 1107 44-45 0.10110276228076032 1107 46-47 0.0654756947744275 1107 48-49 0.07701428801122034 1107 50-51 0.12472353571061845 1107 52-53 0.11644695545068018 1107 54-55 0.18828257685618865 1107 56-57 0.1990298220762483 1107 58-59 0.13213143576118114 1107 60-61 0.13715539573203728 1107 62-63 0.18058669368481972 1107 64-65 0.17014817980932762 1107 66-67 0.17722956923168098 1107 68-69 -0.0535722800211893 1107 70-71 -0.17314039557743044 1107 72-73 -0.11866759405440419 1107 74-75 -0.2747678766056154 1107 76-77 -0.18592822375723372 1108 1 0.10486319296848023 1108 2 0.19812198329785247 1108 3 0.17279416272779002 1108 4 0.19049204539455644 1108 5 0.2241369909968114 1108 6 0.10297650144902804 1108 7 0.1217259225167524 1108 8 0.19804981132507038 1108 9 0.15026844497598546 1108 10-11 0.2316971521800184 1108 12-13 0.29580916460295725 1108 14-15 0.22640496196508053 1108 16-17 0.1660460182388377 1108 18-19 0.11036312289065364 1108 20-21 0.09005176263239889 1108 22-23 0.16414579104323934 1108 24-25 0.15315661000536096 1108 26-27 -0.07420972531288328 1108 28-29 0.15898118319861965 1108 30-31 0.28391034032555496 1108 32-33 0.276000753222597 1108 34-35 0.23104938029099742 1108 36-37 0.3688089762104312 1108 38-39 0.39456558588852886 1108 40-41 0.20707395472132362 1108 42-43 0.21618810733577476 1108 44-45 0.19998728174920188 1108 46-47 0.11445676128546722 1108 48-49 0.14467754657201937 1108 50-51 0.12011418115282169 1108 52-53 0.17649224044831158 1108 54-55 0.32289060680518133 1108 56-57 0.2283090423519596 1108 58-59 0.2301494885273243 1108 60-61 0.2209815645520088 1108 62-63 0.14133471574087153 1108 64-65 0.21112667327566115 1108 66-67 0.2760287098246472 1108 68-69 0.30284102022953974 1108 70-71 0.21624803601104858 1108 72-73 0.33494127053490885 1108 74-75 0.38499015029751504 1108 76-77 0.35300228141531775 1109 1 0.07395840888174376 1109 2 -0.06607709830856123 1109 3 -0.20524148905467143 1109 4 0.02757686821803418 1109 5 0.03083105054581381 1109 6 -0.09822409759856754 1109 7 0.07747495892334655 1109 8 0.13793097205020644 1109 9 0.0721358209294678 1109 10-11 0.005459451721549158 1109 12-13 -0.031723551147294415 1109 14-15 0.046388290455887216 1109 16-17 0.08493015385812441 1109 18-19 -0.020807278607904323 1109 20-21 0.05938442757523177 1109 22-23 0.1496691726262256 1109 24-25 0.07973160075934516 1109 26-27 -0.03725651143088271 1109 28-29 0.1760577432966457 1109 30-31 0.23283276929559094 1109 32-33 0.15747112191021273 1109 34-35 0.16822105562639678 1109 36-37 0.18730120617084367 1109 38-39 0.19670805217645437 1109 40-41 0.26961360563160497 1109 42-43 0.27268011090585986 1109 44-45 0.314077367365428 1109 46-47 0.20060209031564114 1109 48-49 0.17758411152647824 1109 50-51 0.060470657246472825 1109 52-53 0.13935217629743235 1109 54-55 0.1795693041531301 1109 56-57 0.2549220604219755 1109 58-59 0.3962395553646374 1109 60-61 0.31382110962220366 1109 62-63 0.2747633868765753 1109 64-65 0.32336385314069105 1109 66-67 0.3278238964740865 1109 68-69 0.2043450031866243 1109 70-71 0.30044990315748166 1109 72-73 0.3247375870494551 1109 74-75 0.3179165896105616 1109 76-77 0.44794856065892574 1110 1 -0.3468684760616725 1110 2 -0.35491171532911636 1110 3 -0.5546737644859547 1110 4 -0.6058732516970551 1110 5 -0.5802230041019172 1110 6 -0.5044858419887177 1110 7 -0.5429857906008877 1110 8 -0.8029660647190937 1110 9 -0.6116219246268813 1110 10-11 -0.6529459371687807 1110 12-13 -0.5165035703576706 1110 14-15 -0.4776166439352778 1110 16-17 -0.539694025657063 1110 18-19 -0.5690771464716562 1110 20-21 -0.6752076813927346 1110 22-23 -0.5599868378056883 1110 24-25 -0.7098986893333361 1110 26-27 -0.6852970828697238 1110 28-29 -0.47929470323080636 1110 30-31 -0.3084372423464643 1110 32-33 -0.3367866326322897 1110 34-35 -0.2887189428958834 1110 36-37 -0.24065483561273027 1110 38-39 -0.3526068043148385 1110 40-41 -0.2247663604606842 1110 42-43 -0.29403166952871373 1110 44-45 -0.401237267467657 1110 46-47 -0.3366636459190815 1110 48-49 -0.24825644233946065 1110 50-51 -0.2623113129554753 1110 52-53 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18-19 -0.33270953130523395 2314 20-21 -0.40090795068463336 2314 22-23 -0.20337649434816285 2314 24-25 -0.16653555500744943 2314 26-27 -0.19276244878663817 2314 28-29 -0.3861361934965686 2314 30-31 -0.5097650086584835 2314 32-33 -0.6241715765665958 2314 34-35 -0.6527487815966637 2314 36-37 -1.095712646457244 2314 38-39 -0.6448128250304421 2314 40-41 -0.5236496682259997 2314 42-43 -0.5077822834369741 2314 44-45 -0.5396880665140991 2314 46-47 -0.28040085041537566 2314 48-49 -0.29748395260345006 2314 50-51 -0.1487758773427288 2314 52-53 0.03369352286735605 2314 54-55 -0.11772278021393845 2314 56-57 -0.13014110849967153 2314 58-59 -0.11775014756924662 2314 60-61 -0.14617760121612733 2314 62-63 -0.1878876614011773 2314 64-65 -0.10788461428949248 2314 66-67 0.027134735889621453 2314 68-69 -0.02164291836022514 2314 70-71 -0.013038694523107353 2314 72-73 0.008651508913636974 2314 74-75 0.01642575978711136 2314 76-77 0.09258046443517642 2315 1 -1.1610467812146794 2315 2 -1.0479734389250908 2315 3 -1.0031206767680274 2315 4 -0.8852056630870138 2315 5 -1.1920468579184273 2315 6 -0.9599109520346403 2315 7 -1.250586649714144 2315 8 -1.2768133791511929 2315 9 -0.8178895082030522 2315 10-11 -0.9993131982978198 2315 12-13 -0.9518098182252643 2315 14-15 -0.9264440676022829 2315 16-17 -0.9560509114274396 2315 18-19 -0.9752731372764671 2315 20-21 -1.0049904490686004 2315 22-23 -1.109757796869161 2315 24-25 -1.2360646587162378 2315 26-27 -1.1987878457242545 2315 28-29 -1.1370464734739443 2315 30-31 -1.0766876310728364 2315 32-33 -1.1106221664179188 2315 34-35 -1.1903082464883852 2315 36-37 -1.1363501454472242 2315 38-39 -1.0710706779312176 2315 40-41 -1.1167292678543106 2315 42-43 -1.0615222589945006 2315 44-45 -0.9770163632173947 2315 46-47 -0.8963731708339253 2315 48-49 -0.9251491412750745 2315 50-51 -0.8769228452645095 2315 52-53 -0.8680650471471871 2315 54-55 -0.989062091178706 2315 56-57 -1.1029608553888117 2315 58-59 -0.8666271322573493 2315 60-61 -0.9620718976366227 2315 62-63 -0.8919942739981401 2315 64-65 -0.6966388058699735 2315 66-67 -0.6801900509962913 2315 68-69 -0.9796065425118066 2315 70-71 -0.8905814183434053 2315 72-73 -0.7555146443265031 2315 74-75 -0.9513104332477909 2315 76-77 -0.873310549422289 2316 1 -1.872323526402134 2316 2 -1.577668185538478 2316 3 -1.908922379424368 2316 4 -2.6282244720746704 2316 5 -1.9789139208362414 2316 6 -1.9639014645963186 2316 7 -1.6281450461676528 2316 8 -1.4345050691717347 2316 9 -1.7170309444487373 2316 10-11 -1.5882382994195083 2316 12-13 -2.4019933990979183 2316 14-15 -3.569792757432232 2316 16-17 -2.6759526045539914 2316 18-19 -2.1376839278257336 2316 20-21 -2.034930543731214 2316 22-23 -2.507936194593313 2316 24-25 -2.564309288501427 2316 26-27 -2.5188245883704354 2316 28-29 -3.1631734156104514 2316 30-31 -3.2843909244986946 2316 32-33 -3.2344961949084734 2316 34-35 -3.1505425382129957 2316 36-37 -3.0596173555473953 2316 38-39 -3.080963397169402 2316 40-41 -2.8616473734020644 2316 42-43 -3.0638457636993977 2316 44-45 -2.7912376820701468 2316 46-47 -2.8482480252398688 2316 48-49 -2.733223920148582 2316 50-51 -2.747679219637135 2316 52-53 -2.819695303533912 2316 54-55 -2.8525261444467205 2316 56-57 -2.7111086518889067 2316 58-59 -2.853979332227702 2316 60-61 -2.5812877545061887 2316 62-63 -2.6332156140282095 2316 64-65 -2.7268452053024212 2316 66-67 -2.7495655318978756 2316 68-69 -2.782762933473329 2316 70-71 -2.706734196807485 2316 72-73 -2.647947459286158 2316 74-75 -2.7159445774370887 2316 76-77 -3.1613102458807383 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76984.0 3 6644.0 4 8331.0 5 10325.0 6 11581.0 7 12202.0 8 14072.0 9 14995.0 10 17193.0 11 19090.0 12 15798.0 13 14032.0 14 14028.0 15 14964.0 16 14826.0 17 15587.0 18 16607.0 19 17622.0 20 18897.0 21 20345.0 22 22408.0 23 25357.0 24 29760.0 25 35166.0 26 41374.0 27 48464.0 28 57259.0 29 67684.0 30 80658.0 31 96793.0 32 118620.0 33 148314.0 34 198240.0 35 264242.0 36 375461.0 37 512194.0 38 465365.0 39 117620.0 40 608.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.904363844115366 26.749512027078087 28.269810785476253 22.076313343330295 2 22.507118130403562 26.960055774894826 28.16249765888605 22.370328435815562 3 22.68233989711419 27.14474552138159 27.721018502840835 22.451896078663385 4 22.338349750530302 26.962046543922064 28.25949163296655 22.440112072581087 5 22.686204865394586 27.007869279866426 27.982066122348115 22.323859732390876 6 22.790704668063086 26.792331207326225 28.12824425285102 22.288719871759668 7 22.624684511109493 26.612960958648195 28.20608791814434 22.55626661209797 8 22.510034003487302 27.081643020262835 27.84451495179298 22.563808024456883 9 22.453067707568888 26.754634716085334 28.157773701833293 22.634523874512485 10-11 22.91812069654828 26.959487444864006 27.70093235528814 22.42145950329958 12-13 23.066500035468515 26.600079567159877 27.624057264818404 22.7093631325532 14-15 22.70219095150202 26.83314991238613 27.86163643298936 22.60302270312249 16-17 22.907751834608703 26.577198033147813 28.0378904059084 22.47715972633508 18-19 22.894783162808775 26.592531807162633 27.687345074557886 22.825339955470707 20-21 22.786785579454694 26.846457703100455 27.64929879698044 22.71745792046441 22-23 22.97871366744682 26.825665851649426 27.25039552307109 22.945224957832664 24-25 22.96814740050915 26.88566980357223 27.254465289043438 22.891717506875175 26-27 23.03430973968399 26.889846817656217 27.12980661188767 22.946036830772123 28-29 23.205066018531472 26.833470395274432 27.170247273687636 22.791216312506453 30-31 22.878943284428992 26.788904643395757 27.252110025292193 23.080042046883058 32-33 22.859044261394335 27.145041516405378 27.117372629145713 22.878541593054567 34-35 22.972589344856953 26.76407767101363 27.290171620377535 22.97316136375188 36-37 23.041723867424984 26.872850885794602 27.487187030136628 22.598238216643786 38-39 22.805131346599293 26.872574087379597 27.540159743301917 22.782134822719193 40-41 22.72370759263433 26.806895890089912 27.401868499024534 23.06752801825123 42-43 22.699259761445457 27.164602439873086 27.066505427124888 23.069632371556573 44-45 22.705038365269793 26.877149087637832 27.457229090331392 22.960583456760986 46-47 22.753835190590912 26.760444826537917 27.405178528816638 23.080541454054533 48-49 22.76057778128626 26.82385528892742 27.297303967355447 23.11826296243088 50-51 22.679323256467413 27.040367562432277 27.1519289328734 23.12838024822691 52-53 22.738342383930586 26.884115302756374 27.337636862357904 23.039905450955132 54-55 22.79537036234896 26.754564116663154 27.342855466274546 23.107210054713335 56-57 22.7045514424331 27.1936901274383 27.132726596378276 22.969031833750325 58-59 22.8568818162636 27.00150350673865 27.1415246202293 23.000090056768453 60-61 22.757763995459406 26.88130144022412 27.125231585717568 23.235702978598905 62-63 22.775635100245434 26.801912799426585 27.298812387264242 23.12363971306374 64-65 22.75526412224421 26.811514136546 27.21742353934562 23.215798201864168 66-67 22.698237444437453 26.924693185226474 27.36638223763731 23.010687132698767 68-69 22.835326552649793 26.640821999047336 27.398573356039503 23.125278092263372 70-71 22.575751530923576 26.567712833204382 27.521437508298018 23.33509812757402 72-73 22.798815416591037 26.424222801104786 27.445574410120305 23.331387372183876 74-75 22.566854645014647 26.471911023800015 27.35354811993849 23.607686211246843 76-77 22.418723525065474 26.35530568236195 27.731617960909578 23.494352831662997 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5435.0 1 3334.5 2 1157.5 3 1142.0 4 1203.0 5 1249.5 6 1321.5 7 1469.5 8 1624.0 9 1656.0 10 1746.0 11 1870.5 12 2031.0 13 2157.0 14 2264.5 15 2556.0 16 2939.0 17 3138.0 18 3478.5 19 4236.5 20 5120.0 21 6398.0 22 7210.0 23 8372.5 24 11131.5 25 15068.5 26 17409.0 27 20288.5 28 28587.5 29 37435.5 30 40864.0 31 47979.0 32 60993.0 33 73407.5 34 87229.5 35 94536.0 36 102610.5 37 116055.5 38 122475.0 39 123524.0 40 125882.0 41 132077.5 42 141274.5 43 146634.0 44 145111.0 45 143089.0 46 145351.5 47 148449.0 48 148785.0 49 148511.0 50 146982.0 51 140768.0 52 135809.0 53 134175.5 54 125951.0 55 111517.0 56 96609.5 57 89545.0 58 86047.0 59 74709.5 60 61001.5 61 55133.0 62 47957.5 63 34385.0 64 24434.5 65 20881.0 66 18236.0 67 13874.0 68 10425.5 69 7427.0 70 6160.0 71 5162.0 72 3499.0 73 2629.0 74 2424.0 75 1989.5 76 1239.0 77 747.0 78 571.0 79 485.0 80 327.0 81 215.5 82 150.5 83 125.0 84 116.5 85 97.5 86 69.0 87 51.0 88 49.0 89 45.5 90 37.5 91 31.0 92 32.0 93 33.5 94 34.5 95 31.0 96 27.0 97 30.5 98 33.5 99 68.5 100 104.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00372584329887473 2 0.00879168287190616 3 0.01294240303819643 4 0.020296041128080766 5 0.015426298570779583 6 0.014838007523588836 7 0.018596533658418606 8 0.020001895604485394 9 0.018596533658418606 10-11 0.02232237695729334 12-13 0.02153798889437234 14-15 0.008595585856175912 16-17 0.0066019328629183804 18-19 0.01227240490111808 20-21 0.010621921685388485 22-23 0.0153609328988695 24-25 0.02279627807864144 26-27 0.01462556908988107 28-29 0.008693634364041037 30-31 0.00875900003595112 32-33 0.010360458997748153 34-35 0.012108990721342873 36-37 0.010131679146062863 38-39 0.0180409254471829 40-41 0.01338362132358949 42-43 0.016128979543812975 44-45 0.018400436642688357 46-47 0.017648731415722405 48-49 0.0148870317775214 50-51 0.027960166159537996 52-53 0.03202917923594066 54-55 0.025051393759539305 56-57 0.01647214932134091 58-59 0.017272878802239426 60-61 0.02083530792133895 62-63 0.027535289292122456 64-65 0.021341891878642095 66-67 0.02148896464043978 68-69 0.030166257586503296 70-71 0.0572603285932327 72-73 0.06847054132581191 74-75 0.07984416823816637 76-77 0.07637978762693197 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3059710.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.10141888574196 #Duplication Level Percentage of deduplicated Percentage of total 1 74.14251058941231 37.88787490870118 2 13.417577040140616 13.713144495198787 3 5.093883061086746 7.8091395617873784 4 2.4672484364424916 5.043195833833587 5 1.3241634517836238 3.3833315611392467 6 0.8218348657952462 2.519815663914624 7 0.5457318629981768 1.952137077125632 8 0.36908785192155114 1.5088730341345509 9 0.2632511036083297 1.21072544258608 >10 1.3658859269769814 12.579374644867466 >50 0.11591659252862027 4.114050987875681 >100 0.06867447517143214 6.3852513820076755 >500 0.0032723007400740174 1.1231556993998244 >1k 8.982786342947601E-4 0.5788407739945023 >5k 6.416275959248285E-5 0.1910889334337804 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5828 0.1904755679459818 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.536567191008299E-5 0.0 2 0.0 0.0 0.0 2.2877985168529043E-4 0.0 3 0.0 0.0 0.0 4.902425393256224E-4 0.0 4 0.0 0.0 0.0 9.151194067411617E-4 0.0 5 0.0 0.0 0.0 0.0013726791101117426 0.0 6 0.0 0.0 0.0 0.0029414552359537344 0.0 7 0.0 3.2682835955041494E-5 0.0 0.006013641815727634 0.0 8 0.0 3.2682835955041494E-5 0.0 0.009249242575276742 0.0 9 0.0 3.2682835955041494E-5 0.0 0.012975085874151472 0.0 10 0.0 3.2682835955041494E-5 0.0 0.017877511267407695 0.0 11 0.0 3.2682835955041494E-5 0.0 0.023139447856169375 0.0 12 0.0 3.2682835955041494E-5 0.0 0.031244791173019665 0.0 13 0.0 3.2682835955041494E-5 0.0 0.04314134346065477 0.0 14 0.0 3.2682835955041494E-5 0.0 0.05631252635053649 0.0 15 0.0 3.2682835955041494E-5 0.0 0.07036614581120433 0.0 16 0.0 3.2682835955041494E-5 0.0 0.08363537720895117 0.0 17 0.0 3.2682835955041494E-5 0.0 0.09739485114602364 0.0 18 0.0 3.2682835955041494E-5 0.0 0.11259236986511793 0.0 19 0.0 3.2682835955041494E-5 0.0 0.12798598559994248 0.0 20 0.0 3.2682835955041494E-5 0.0 0.14586349686735017 0.0 21 0.0 3.2682835955041494E-5 0.0 0.166192220831386 0.0 22 0.0 3.2682835955041494E-5 0.0 0.18701118733474742 0.0 23 0.0 3.2682835955041494E-5 0.0 0.21018331802687182 0.0 24 0.0 3.2682835955041494E-5 0.0 0.23342081439090634 0.0 25 0.0 3.2682835955041494E-5 0.0 0.2582924525526929 0.0 26 0.0 3.2682835955041494E-5 0.0 0.2862689601302084 0.0 27 0.0 3.2682835955041494E-5 0.0 0.31182693784705084 0.0 28 0.0 3.2682835955041494E-5 0.0 0.3393458857211958 0.0 29 0.0 3.2682835955041494E-5 0.0 0.3662111768762399 0.0 30 0.0 3.2682835955041494E-5 0.0 0.39735791954139443 0.0 31 0.0 3.2682835955041494E-5 0.0 0.43062904654362666 0.0 32 0.0 3.2682835955041494E-5 0.0 0.46550163250765597 0.0 33 0.0 3.2682835955041494E-5 0.0 0.5038385990829196 0.0 34 0.0 3.2682835955041494E-5 0.0 0.5428945880491942 0.0 35 0.0 3.2682835955041494E-5 0.0 0.5850881292671528 0.0 36 0.0 3.2682835955041494E-5 0.0 0.6277065473525268 0.0 37 0.0 3.2682835955041494E-5 0.0 0.6738220288850905 0.0 38 0.0 3.2682835955041494E-5 0.0 0.7209506783322602 0.0 39 0.0 3.2682835955041494E-5 0.0 0.7682754247951603 0.0 40 0.0 3.2682835955041494E-5 0.0 0.8218752757614284 0.0 41 0.0 3.2682835955041494E-5 0.0 0.877534145392864 0.0 42 0.0 3.2682835955041494E-5 0.0 0.9370495896669946 0.0 43 0.0 3.2682835955041494E-5 0.0 1.0011079481388758 0.0 44 0.0 3.2682835955041494E-5 0.0 1.0730428700759223 0.0 45 0.0 3.2682835955041494E-5 0.0 1.1455987658961144 0.0 46 0.0 3.2682835955041494E-5 0.0 1.224887325923045 0.0 47 0.0 3.2682835955041494E-5 0.0 1.302835889675819 0.0 48 0.0 3.2682835955041494E-5 0.0 1.3820917668667945 0.0 49 0.0 3.2682835955041494E-5 0.0 1.4671325060218126 0.0 50 0.0 3.2682835955041494E-5 0.0 1.5541342153341329 0.0 51 0.0 3.2682835955041494E-5 0.0 1.65025443587791 0.0 52 0.0 3.2682835955041494E-5 0.0 1.750329279572247 0.0 53 0.0 3.2682835955041494E-5 0.0 1.85899970912276 0.0 54 0.0 3.2682835955041494E-5 0.0 1.9779979148350662 0.0 55 0.0 3.2682835955041494E-5 0.0 2.1013429377293926 0.0 56 0.0 3.2682835955041494E-5 0.0 2.2320415987136037 0.0 57 0.0 3.2682835955041494E-5 0.0 2.3609753865562424 0.0 58 0.0 3.2682835955041494E-5 0.0 2.4996486595134835 0.0 59 0.0 3.2682835955041494E-5 0.0 2.6413941190504984 0.0 60 0.0 3.2682835955041494E-5 0.0 2.785198597252681 0.0 61 0.0 3.2682835955041494E-5 0.0 2.9377620754908147 0.0 62 0.0 3.2682835955041494E-5 0.0 3.0971235836075968 0.0 63 0.0 3.2682835955041494E-5 0.0 3.272140170146844 0.0 64 0.0 3.2682835955041494E-5 0.0 3.4534645440254144 0.0 65 0.0 3.2682835955041494E-5 0.0 3.638253298515219 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAC 395 0.0 32.350094 4 GGGCGGT 630 0.0 21.408781 1 ATTCGCG 535 0.0 19.90615 56 CGCGGAA 510 0.0 19.491726 59 CGATCGT 570 0.0 18.680792 1 TCGCGCT 550 0.0 18.071192 37 AATTCGC 595 0.0 17.89881 55 GACGAAT 475 0.0 17.191105 47 CTCGCGC 585 0.0 16.9889 36 GACGTAT 505 0.0 16.871233 39 CCTCGCG 650 0.0 16.38135 35 ACTCGAG 655 0.0 16.256832 14 GCGGTTA 790 0.0 16.174519 3 GCCTAGT 815 0.0 16.113878 17 CAATTCG 620 0.0 16.032469 54 CGCGCTT 650 0.0 15.838151 38 GCGTAAG 660 0.0 15.59818 38 CGCTCCG 690 0.0 15.433471 25 CGGTTCA 580 0.0 15.300424 10 TCGCGGA 650 0.0 15.293259 58 >>END_MODULE