FastQCFastQC Report
Thu 26 May 2016
SRR936116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936116_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059710
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70770.23129643005382863No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50190.16403515365835322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35180.11497821688983598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA15450.046.421043100-101
CGGTATC10100.039.188316100-101
GGTATCA88200.038.458751
CGTACAT19350.036.911068100-101
TCGTATC7100.031.855291100-101
ACGTATC5000.030.35474100-101
GTATCAA171750.030.0061341
CGAGTAC11600.029.503100-101
TACGCAG10150.029.026556100-101
TCGAGTA3750.028.569166100-101
AGGTATC22450.028.235119100-101
CCGTACA10150.028.146963100-101
TCGGTAT4800.027.899576100-101
GGGTATC15250.027.71053100-101
CCGTATC7550.027.591635100-101
CCGGTAT4900.027.330196100-101
ACCGTAT3600.027.2775898-99
TCGTACA10200.027.133705100-101
CCGTACG1900.026.62326696-97
AGTATCA36500.026.25363100-101
Produced by FastQC (version 0.11.5)