FastQCFastQC Report
Thu 26 May 2016
SRR936115_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936115_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2979697
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62300.20908166165888678No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA3300.021.51571759
ATTCGCG3150.021.4125456
CTCGCGC3450.020.57713936
CAATTCG3700.020.15019454
CGCGCTT3700.020.14579638
TCGCGCT3550.019.99817537
CGATCGT3650.019.4479941
AATTCGC4050.018.40634555
TCGCGGA3700.018.2292958
ATCGTTC4050.017.5266153
CCTCGCG4200.016.90236935
TTGCGCG2953.583409E-1016.8433765
TCGCGGG2752.5465852E-916.7856463
GATCGTT4550.016.3806462
ATCGCCC2854.105459E-916.1923257
ACGATCG2201.2042492E-616.13678759
ACGACGG1553.6418508E-416.03267924
CCGCACG1553.6451107E-416.03079417
ATCGCGG2301.9105119E-615.43777862
GATCGCG2352.3895136E-615.10931561
Produced by FastQC (version 0.11.5)