##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936115_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2979697 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.54927162057081 39.0 36.0 41.0 30.0 41.0 2 36.41216338439781 39.0 36.0 41.0 30.0 41.0 3 36.32188977604099 39.0 36.0 41.0 29.0 41.0 4 36.20506917314076 39.0 36.0 41.0 29.0 41.0 5 36.135206700547066 39.0 36.0 41.0 29.0 41.0 6 36.040703131895626 39.0 35.0 40.0 29.0 41.0 7 35.87273974501434 39.0 35.0 40.0 28.0 41.0 8 35.691276663365436 39.0 35.0 40.0 27.0 41.0 9 35.60645562283682 38.0 35.0 40.0 27.0 41.0 10-11 35.80954858832962 39.0 35.0 40.0 28.0 41.0 12-13 35.972268321242055 39.0 35.0 41.0 29.0 41.0 14-15 35.91770169919962 39.0 35.0 41.0 28.0 41.0 16-17 35.75643345615343 39.0 35.0 41.0 27.0 41.0 18-19 35.605828210049545 39.0 35.0 41.0 27.0 41.0 20-21 35.403334969965066 39.0 35.0 41.0 26.0 41.0 22-23 35.151783889435734 39.0 35.0 41.0 25.0 41.0 24-25 34.926845078543224 39.0 34.5 41.0 24.0 41.0 26-27 34.000310266446554 38.0 33.5 40.0 23.0 40.5 28-29 34.03709555031938 38.0 33.5 40.0 23.0 40.5 30-31 34.42253037808878 38.0 34.0 40.0 23.0 41.0 32-33 34.276419213094485 38.0 34.0 40.0 22.5 41.0 34-35 34.01731484778486 38.0 33.0 40.0 21.5 41.0 36-37 33.74276948293736 38.0 33.0 40.0 20.5 41.0 38-39 33.47622174335176 37.0 33.0 40.0 19.5 41.0 40-41 33.106313326489236 37.0 32.5 40.0 18.0 41.0 42-43 32.75459014792443 36.0 32.0 39.5 16.0 41.0 44-45 32.395384497148534 36.0 32.0 39.0 13.0 41.0 46-47 32.01069924223839 35.5 31.5 39.0 9.5 40.0 48-49 31.54753016833591 35.0 31.0 38.0 7.5 40.0 50-51 31.10600507367024 35.0 31.0 37.0 4.5 39.5 52-53 30.644925473965976 35.0 30.5 37.0 2.0 39.0 54-55 30.172089980961154 35.0 30.0 36.0 2.0 39.0 56-57 29.709947689312035 34.0 29.5 36.0 2.0 37.5 58-59 29.270531533910997 34.0 29.0 35.0 2.0 37.0 60-61 28.906412464086113 34.0 29.0 35.0 2.0 37.0 62-63 28.555632837835525 34.0 29.0 35.0 2.0 36.0 64-65 28.173857778156638 34.0 28.5 35.0 2.0 36.0 66-67 27.781925142053034 34.0 27.0 35.0 2.0 35.5 68-69 27.414255543432773 34.0 26.5 35.0 2.0 35.0 70-71 26.99721716671192 33.0 26.0 35.0 2.0 35.0 72-73 26.515007062798666 33.0 25.0 35.0 2.0 35.0 74-75 25.93958865616202 33.0 24.5 35.0 2.0 35.0 76-77 24.235153272295808 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1315895091231383 1101 2 1.0747670385923485 1101 3 1.2531780835729336 1101 4 1.1845713150838506 1101 5 1.2097648294454615 1101 6 1.125454968681808 1101 7 1.067782867103574 1101 8 1.1752343090163109 1101 9 1.1955965963595503 1101 10-11 1.1651053707158923 1101 12-13 1.163332250774296 1101 14-15 1.2247356313131803 1101 16-17 1.2636680719458724 1101 18-19 1.1854440661411658 1101 20-21 1.17412547015266 1101 22-23 1.1240109754311973 1101 24-25 1.2614825331890316 1101 26-27 1.1405125565887815 1101 28-29 1.2317988243808173 1101 30-31 1.2541283149227453 1101 32-33 1.337100506568845 1101 34-35 1.3202807382394397 1101 36-37 1.322780004676268 1101 38-39 1.3635714592212125 1101 40-41 1.2406702353942691 1101 42-43 1.2688236948634213 1101 44-45 1.1704244425553867 1101 46-47 1.1561730341904237 1101 48-49 1.3673544206496224 1101 50-51 1.283471774081594 1101 52-53 1.37787345353237 1101 54-55 1.271573330075352 1101 56-57 1.2310223303106902 1101 58-59 1.1986123573234373 1101 60-61 1.1833460100429072 1101 62-63 1.1164810844283828 1101 64-65 1.0328084110951572 1101 66-67 0.9813742144364035 1101 68-69 1.009584842723168 1101 70-71 1.1511739117963877 1101 72-73 1.3183935191914138 1101 74-75 1.1733223294516364 1101 76-77 1.2527539407501074 1102 1 0.9991558287216407 1102 2 1.0108650482410084 1102 3 0.9933104320196975 1102 4 1.163274191971169 1102 5 1.064276994355815 1102 6 1.123284033562662 1102 7 1.0404958907759507 1102 8 1.1424678191629667 1102 9 1.1569353113603285 1102 10-11 1.0395298777482012 1102 12-13 1.2250753874091203 1102 14-15 1.1437826662050696 1102 16-17 1.1867284558583293 1102 18-19 1.3108034214881386 1102 20-21 1.2896092081864268 1102 22-23 1.3356028024552486 1102 24-25 1.4351138629478655 1102 26-27 1.2574565511890583 1102 28-29 1.3547025350707855 1102 30-31 1.431266437748917 1102 32-33 1.3260257768537187 1102 34-35 1.5147690828721991 1102 36-37 1.5305989822497352 1102 38-39 1.658243175648657 1102 40-41 1.5742015178630453 1102 42-43 1.53065530453906 1102 44-45 1.506252983820275 1102 46-47 1.451185751526232 1102 48-49 1.437448926425997 1102 50-51 1.5757700259424574 1102 52-53 1.5933815800679447 1102 54-55 1.5446897663855133 1102 56-57 1.568855455073873 1102 58-59 1.5788546571551798 1102 60-61 1.4790510183020693 1102 62-63 1.5043268349211303 1102 64-65 1.497367881998759 1102 66-67 1.5062876598161203 1102 68-69 1.4439818307100083 1102 70-71 1.3940255051740813 1102 72-73 1.4785807703112006 1102 74-75 1.4025557923347804 1102 76-77 1.6159621266836872 1103 1 0.708032141474618 1103 2 0.7075330849778965 1103 3 0.7685310093657094 1103 4 0.7191108891040798 1103 5 0.7851295423300471 1103 6 0.8016865792006485 1103 7 0.8164822819397131 1103 8 0.8851318058772435 1103 9 0.7743990341975007 1103 10-11 0.6988512257389061 1103 12-13 0.8090276281866835 1103 14-15 0.8749443986390659 1103 16-17 0.8918711292692194 1103 18-19 0.8990583163213941 1103 20-21 0.8318158844673462 1103 22-23 0.9232379978961589 1103 24-25 0.8529454852773171 1103 26-27 0.7258683151029857 1103 28-29 0.7009689749235122 1103 30-31 0.9042990208698143 1103 32-33 0.8325486574224072 1103 34-35 0.9743391421653556 1103 36-37 0.9292785275764857 1103 38-39 0.855548730610245 1103 40-41 0.9493796071932081 1103 42-43 0.9039077720513689 1103 44-45 0.8931865528373635 1103 46-47 0.7031614640617434 1103 48-49 0.7164488781171663 1103 50-51 0.8565486930779578 1103 52-53 0.9367334529064166 1103 54-55 0.9577778175516976 1103 56-57 0.9315855060990899 1103 58-59 0.9222659261015451 1103 60-61 0.9676667642894685 1103 62-63 0.9900333595439861 1103 64-65 0.8950800627149782 1103 66-67 0.8092634444189066 1103 68-69 0.7136633250210451 1103 70-71 0.8052813843988282 1103 72-73 0.8423391659510528 1103 74-75 0.6303202653843911 1103 76-77 0.7887008574601388 1104 1 0.531467860299415 1104 2 0.6926098888361523 1104 3 0.49401787300030975 1104 4 0.3695913634185004 1104 5 0.5951264123962687 1104 6 0.3235770181886011 1104 7 0.5949675631761835 1104 8 0.5389053755474933 1104 9 0.4610413945315699 1104 10-11 0.5672062366193344 1104 12-13 0.6289778030246325 1104 14-15 0.5580421922792809 1104 16-17 0.7034903867956075 1104 18-19 0.5628244110637937 1104 20-21 0.6092879105239817 1104 22-23 0.6470489627129936 1104 24-25 0.7098026036721237 1104 26-27 0.5048376338053586 1104 28-29 0.38143010055639337 1104 30-31 0.6145823785684428 1104 32-33 0.682308759918115 1104 34-35 0.7186504042105355 1104 36-37 0.6955012486399141 1104 38-39 0.7334424774332149 1104 40-41 0.6941235742522522 1104 42-43 0.6529471310327324 1104 44-45 0.6805010158230189 1104 46-47 0.6193433242370148 1104 48-49 0.7323072733506386 1104 50-51 0.7172790083963569 1104 52-53 0.7741636414125104 1104 54-55 0.719810182758831 1104 56-57 0.6055776533233832 1104 58-59 0.6596735821605328 1104 60-61 0.6273174831616863 1104 62-63 0.6753626088392366 1104 64-65 0.5777945612296058 1104 66-67 0.6052305337163482 1104 68-69 0.5509511797244429 1104 70-71 0.6438305050053508 1104 72-73 0.6892327259008191 1104 74-75 0.749607847852765 1104 76-77 0.6209323695103812 1105 1 0.15462475104570927 1105 2 0.2903445226162802 1105 3 0.11232540908726207 1105 4 0.286399354456762 1105 5 0.3287013694550396 1105 6 0.039222629555766275 1105 7 0.13493319518965308 1105 8 0.3295449944612159 1105 9 0.13847347099390106 1105 10-11 0.20974022730324293 1105 12-13 0.22592813499466757 1105 14-15 0.04756086916555802 1105 16-17 0.1665761858500474 1105 18-19 0.2818609946013453 1105 20-21 0.2946366525588928 1105 22-23 0.23511958526169252 1105 24-25 0.3113090133153662 1105 26-27 0.17461700023454085 1105 28-29 0.2591752975596293 1105 30-31 0.23274061910158395 1105 32-33 0.35033903015433054 1105 34-35 0.4007919801588997 1105 36-37 0.42295577570248355 1105 38-39 0.3134355103073645 1105 40-41 0.4026715824632632 1105 42-43 0.15676054460054445 1105 44-45 0.16628173966638116 1105 46-47 -0.02310163928247988 1105 48-49 0.09695328596523822 1105 50-51 0.16020034226835023 1105 52-53 0.13666368673883866 1105 54-55 0.10914879220203844 1105 56-57 0.19142203409006342 1105 58-59 0.12706975891896732 1105 60-61 -0.25366370122219806 1105 62-63 -0.20511048301291268 1105 64-65 0.09998364417354111 1105 66-67 0.219177301723942 1105 68-69 -0.24363132976376178 1105 70-71 -0.48699872676398925 1105 72-73 -0.5915662653913998 1105 74-75 -0.5149714444037876 1105 76-77 -0.24966339602257293 1106 1 0.3046001832672047 1106 2 0.41735574761523253 1106 3 0.145800435654742 1106 4 0.37799298435555784 1106 5 0.1212529522675041 1106 6 0.4186847285201125 1106 7 0.6512001117239592 1106 8 0.5578284114011751 1106 9 0.37071172892891724 1106 10-11 0.3033373690612322 1106 12-13 0.24072346064152583 1106 14-15 0.3418301015462717 1106 16-17 0.368634546703106 1106 18-19 0.5227036884487362 1106 20-21 0.33713014876326497 1106 22-23 0.46858318495545603 1106 24-25 0.4798119785890975 1106 26-27 0.38078198905525085 1106 28-29 0.427115108406781 1106 30-31 0.37499853125939353 1106 32-33 0.40757852600065547 1106 34-35 0.46657096918529106 1106 36-37 0.3619699793065152 1106 38-39 0.34442004974722096 1106 40-41 0.3163613580775504 1106 42-43 0.38323051668088226 1106 44-45 0.1273983737664537 1106 46-47 0.3181156655750854 1106 48-49 0.14004974020632588 1106 50-51 0.06409900113427369 1106 52-53 0.1613685877249793 1106 54-55 0.3260081921388114 1106 56-57 0.23120717077451758 1106 58-59 0.06695868970638941 1106 60-61 -0.005923555948545101 1106 62-63 -0.00561402724824589 1106 64-65 0.025644403146493744 1106 66-67 0.07533391083725505 1106 68-69 0.028260274912113914 1106 70-71 -0.07003077152686288 1106 72-73 -0.02579984592610174 1106 74-75 -0.09890525995118793 1106 76-77 0.006604434661628744 1107 1 0.2963691952399543 1107 2 0.38363336737332787 1107 3 0.21449489908604846 1107 4 0.2871630257836699 1107 5 0.25872738125296735 1107 6 0.23311621290066142 1107 7 0.21851380161829326 1107 8 0.40060274704477905 1107 9 0.30146039923615575 1107 10-11 0.35046900558165817 1107 12-13 0.49168309334989146 1107 14-15 0.37679667242115755 1107 16-17 0.4004458687521151 1107 18-19 0.39783310534385663 1107 20-21 0.3029388543003293 1107 22-23 0.42124673532698154 1107 24-25 0.2781248428612102 1107 26-27 0.2256781982978069 1107 28-29 0.35534171108159285 1107 30-31 0.5474721150232966 1107 32-33 0.6413826109931975 1107 34-35 0.5702817368179538 1107 36-37 0.5030991953084012 1107 38-39 0.481826248528634 1107 40-41 0.5770017175294484 1107 42-43 0.5961060584188971 1107 44-45 0.6543087582320197 1107 46-47 0.5728757328988152 1107 48-49 0.693831555448245 1107 50-51 0.5188981446430923 1107 52-53 0.6419046051879924 1107 54-55 0.7138953097373033 1107 56-57 0.724101307386821 1107 58-59 0.8269249543415853 1107 60-61 0.6928649598179 1107 62-63 0.8085828775259074 1107 64-65 0.7226339444484289 1107 66-67 0.7033347536088961 1107 68-69 0.5074924462495503 1107 70-71 0.5974626198205151 1107 72-73 0.7071475028956122 1107 74-75 0.680533439436573 1107 76-77 0.6092899117953223 1108 1 -0.1248586996721528 1108 2 -0.09565050664738806 1108 3 -0.22666919997278967 1108 4 -0.34516955391733717 1108 5 -0.2568491474814607 1108 6 -0.16289566995332194 1108 7 -0.34758676448227277 1108 8 -0.05859156741269089 1108 9 -0.16521327796383645 1108 10-11 -0.4092756357419276 1108 12-13 -0.2872331658295266 1108 14-15 -0.16729081903475418 1108 16-17 -0.12438237318139045 1108 18-19 -0.31560258177604084 1108 20-21 -0.3990411832060303 1108 22-23 -0.4303687544463983 1108 24-25 -0.2489993487366604 1108 26-27 -0.3528923539569604 1108 28-29 -0.20084333194554915 1108 30-31 -0.1446603139512348 1108 32-33 -0.1186621687884255 1108 34-35 -0.10025386792816704 1108 36-37 0.021458029456709937 1108 38-39 0.05329863579049032 1108 40-41 -0.0860304257658484 1108 42-43 0.012452869502553199 1108 44-45 -0.20893983527972892 1108 46-47 -0.2546782920236268 1108 48-49 -0.17929312688697152 1108 50-51 -0.16806401600327447 1108 52-53 -0.2817988843049726 1108 54-55 -0.14398803498814416 1108 56-57 -0.2772788913617994 1108 58-59 -0.2802617186345593 1108 60-61 -0.24885340953204604 1108 62-63 -0.25979611190571106 1108 64-65 -0.31816735951077035 1108 66-67 -0.5142700100485023 1108 68-69 -0.5545332473550921 1108 70-71 -0.6024295932295907 1108 72-73 -0.5522444209699948 1108 74-75 -0.7097254245065017 1108 76-77 -0.7818803214801733 1109 1 -0.07877312705134187 1109 2 -0.05524345384137064 1109 3 -0.22876523494984724 1109 4 0.16504990610279435 1109 5 -0.02282684229313503 1109 6 -0.08858560307714924 1109 7 -0.14233382886102675 1109 8 0.19547577204837552 1109 9 0.1350308374296887 1109 10-11 -0.017463864371983107 1109 12-13 0.021400130272219542 1109 14-15 0.12758683723239272 1109 16-17 0.21067925118475728 1109 18-19 0.21621869912171832 1109 20-21 0.3146987011978766 1109 22-23 0.3210221546700822 1109 24-25 0.2069406596474792 1109 26-27 0.27949288695577224 1109 28-29 0.3838845445693835 1109 30-31 0.4270308814826933 1109 32-33 0.48492427835257956 1109 34-35 0.44542750286932886 1109 36-37 0.48321195588822974 1109 38-39 0.6275732998387653 1109 40-41 0.5081299598295672 1109 42-43 0.5890334064813914 1109 44-45 0.42542268874026945 1109 46-47 0.34938140417790464 1109 48-49 0.41097974685670025 1109 50-51 0.4037275265190381 1109 52-53 0.4326871557269527 1109 54-55 0.41865902742117456 1109 56-57 0.4989848489211788 1109 58-59 0.5845112015313596 1109 60-61 0.45290625168868104 1109 62-63 0.4774265151435486 1109 64-65 0.5416135274637099 1109 66-67 0.4377452080094706 1109 68-69 0.5039494108861717 1109 70-71 0.5245598815800072 1109 72-73 0.37178310320474495 1109 74-75 0.2405157836349332 1109 76-77 0.2853219829391307 1110 1 -0.3279704593934625 1110 2 -0.398458622643993 1110 3 -0.5073003395390572 1110 4 -0.27405127670970586 1110 5 -0.23733461129005207 1110 6 -0.4090576237346184 1110 7 -0.6033510260604871 1110 8 -0.33747041330500593 1110 9 -0.32209063832956986 1110 10-11 -0.37046233086375224 1110 12-13 -0.26991357361703194 1110 14-15 -0.4097598792822694 1110 16-17 -0.37117332193071917 1110 18-19 -0.27068632572147777 1110 20-21 -0.44076015714654204 1110 22-23 -0.6350229249053498 1110 24-25 -0.437963298563524 1110 26-27 -0.3891229871888129 1110 28-29 -0.4348503829660615 1110 30-31 -0.40605610740027487 1110 32-33 -0.28351508073436094 1110 34-35 -0.14497462554673035 1110 36-37 -0.23496988065847546 1110 38-39 -0.20540594841899207 1110 40-41 -0.21434736076883354 1110 42-43 -0.28207435120275903 1110 44-45 -0.2523934491892845 1110 46-47 -0.3184434742098645 1110 48-49 -0.3561315967945937 1110 50-51 -0.42668098552905676 1110 52-53 -0.4560461826342781 1110 54-55 -0.3191291338593558 1110 56-57 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-0.8285905234767768 2314 30-31 -0.9288301601782081 2314 32-33 -1.0088600671361974 2314 34-35 -0.9690951481625589 2314 36-37 -1.051293338639816 2314 38-39 -0.9893188993846351 2314 40-41 -0.7836926633307115 2314 42-43 -0.7551346983880549 2314 44-45 -0.8508249288839309 2314 46-47 -0.772507826453726 2314 48-49 -0.7650379838838894 2314 50-51 -0.7018719122827619 2314 52-53 -0.717643184693145 2314 54-55 -0.745700826704951 2314 56-57 -0.488141446368477 2314 58-59 -0.5269247515180133 2314 60-61 -0.491733679803243 2314 62-63 -0.5376311198646135 2314 64-65 -0.45436024026668775 2314 66-67 -0.5439911857487765 2314 68-69 -0.38722095568342496 2314 70-71 -0.33881261911972516 2314 72-73 -0.3291459865820663 2314 74-75 -0.4446000875530949 2314 76-77 -0.4253219141285065 2315 1 -1.2720082773431614 2315 2 -1.1695356790202993 2315 3 -1.2448925188248197 2315 4 -1.235446576849128 2315 5 -1.048984226240421 2315 6 -1.0463510046569198 2315 7 -1.1327493162438387 2315 8 -1.1964671041032915 2315 9 -1.0190021874210515 2315 10-11 -0.9929285477127294 2315 12-13 -1.1259228895030802 2315 14-15 -1.5707868743368252 2315 16-17 -1.2934474447525304 2315 18-19 -1.1445287232107617 2315 20-21 -1.237349701507604 2315 22-23 -1.3295237932242898 2315 24-25 -1.277712564912683 2315 26-27 -1.221601487117269 2315 28-29 -1.1324234727205749 2315 30-31 -1.4011728903047995 2315 32-33 -1.2073770322568436 2315 34-35 -1.143262383373397 2315 36-37 -1.2995449681459164 2315 38-39 -1.2245837444022456 2315 40-41 -1.248999439452458 2315 42-43 -1.386351337079649 2315 44-45 -1.2638925411629103 2315 46-47 -1.203700070324679 2315 48-49 -1.1342264252397918 2315 50-51 -1.137085332592921 2315 52-53 -1.1449427320591568 2315 54-55 -1.2593253053894315 2315 56-57 -1.2698828028399838 2315 58-59 -1.1502711758928008 2315 60-61 -1.202630668601838 2315 62-63 -1.2184172812172456 2315 64-65 -1.2544497179482939 2315 66-67 -1.2600284766814411 2315 68-69 -1.2573249188638584 2315 70-71 -1.2057245106086825 2315 72-73 -2.6097681355243516 2315 74-75 -3.1623108910052267 2315 76-77 -2.730004794962877 2316 1 -3.5486853603409187 2316 2 -3.5707877484357695 2316 3 -3.059542074997502 2316 4 -2.8867335463429953 2316 5 -3.673909923997286 2316 6 -3.226417353293357 2316 7 -3.6705325830033146 2316 8 -3.421392573786129 2316 9 -2.9802297920953933 2316 10-11 -3.0602409394785397 2316 12-13 -2.988806254628976 2316 14-15 -3.0135621019618313 2316 16-17 -4.406915510919465 2316 18-19 -4.1995544817968735 2316 20-21 -3.500715718799988 2316 22-23 -3.7736293101545577 2316 24-25 -3.627053036583085 2316 26-27 -3.4626025173466743 2316 28-29 -3.591617512309856 2316 30-31 -3.828591513252718 2316 32-33 -4.030119320289824 2316 34-35 -4.026661815412041 2316 36-37 -3.9973109544962533 2316 38-39 -4.072681165084507 2316 40-41 -3.933649743517904 2316 42-43 -4.209518555542928 2316 44-45 -3.902463860720381 2316 46-47 -3.595647669555653 2316 48-49 -3.8657019435356546 2316 50-51 -4.261186732786914 2316 52-53 -5.37069387725213 2316 54-55 -4.419109220449343 2316 56-57 -4.253708932882663 2316 58-59 -4.487856759485307 2316 60-61 -3.9526840046644587 2316 62-63 -4.009577825474107 2316 64-65 -4.1778420264746785 2316 66-67 -4.266235749031008 2316 68-69 -4.292568961403845 2316 70-71 -4.112736494663647 2316 72-73 -4.036475880224746 2316 74-75 -4.084059783587016 2316 76-77 -4.019978071338517 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75742.0 3 6507.0 4 8578.0 5 10109.0 6 11545.0 7 12484.0 8 14106.0 9 15053.0 10 17700.0 11 20179.0 12 16219.0 13 13697.0 14 13699.0 15 14187.0 16 14437.0 17 15418.0 18 16258.0 19 17034.0 20 18268.0 21 19640.0 22 21888.0 23 24797.0 24 28695.0 25 33581.0 26 39798.0 27 46932.0 28 55124.0 29 65310.0 30 77508.0 31 93153.0 32 113460.0 33 142356.0 34 187290.0 35 249511.0 36 353357.0 37 491820.0 38 469696.0 39 133406.0 40 1155.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.185205410302046 26.988578676369652 29.0164518211052 21.8097640922231 2 22.0151094779282 27.013763534119505 28.961421424068966 22.009705563883326 3 22.10836278369433 27.321622108765563 28.549290153716957 22.020724953823155 4 21.799564053696447 27.277960410382963 28.816493726978393 22.1059818089422 5 22.118120120563614 27.356495062664887 28.76368591701518 21.761698899756322 6 22.296841693313414 27.186294750006123 28.69984792306404 21.81701563361642 7 22.084808263052498 27.01875446003307 28.82592797218618 22.070509304728255 8 21.890573649020826 27.348061940305268 28.703765552460382 22.057598858213524 9 21.94634997282825 27.06987822557555 28.76053513748839 22.223236664107812 10-11 22.34199103829018 27.213229817271483 28.552568729211558 21.892210415226785 12-13 22.341779406757002 27.047585545884356 28.47316986964269 22.137465177715953 14-15 22.090511490813626 27.25135066104105 28.610721313577226 22.047416534568097 16-17 22.390163368815607 27.098327379346177 28.582142200276824 21.929367051561393 18-19 22.18554350000604 27.10323539560656 28.448827392309006 22.262393712078392 20-21 22.24671947375965 27.222602474170188 28.360628112811632 22.170049939258526 22-23 22.305370352498876 27.275147171743026 28.057668665321177 22.36181381043692 24-25 22.278644342930875 27.391678982898032 27.98623155544352 22.343445118727576 26-27 22.368838672502903 27.48841925300133 27.8573381544555 22.28540392004027 28-29 22.43785335859531 27.47238160127138 27.81585160994749 22.273913430185825 30-31 22.279473634572273 27.3262758810264 27.946205525783107 22.448044958618215 32-33 22.27981422356105 27.586441844170988 27.77621239264782 22.35753153962014 34-35 22.336156259298363 27.37598786046177 27.912966411695418 22.374889468544456 36-37 22.374726933942053 27.42611126578631 27.992876997302947 22.20628480296869 38-39 22.22802526584028 27.37579673206223 28.099965109013773 22.296212893083712 40-41 22.167430476726224 27.348271207057078 27.993778533793478 22.490519782423217 42-43 22.12037522182589 27.52611310695205 27.828538112431616 22.52497355879045 44-45 22.276617822509305 27.3287683992125 27.978097580123958 22.416516198154234 46-47 22.107906242919125 27.233142272090234 28.008845324314567 22.650106160676074 48-49 22.162539759480214 27.244418567062546 27.94649827048044 22.6465434029768 50-51 22.111162216059785 27.426425660205062 27.780717244898646 22.68169487883651 52-53 22.06549110776755 27.33981047340019 27.910596061508937 22.684102357323326 54-55 22.10004518485844 27.273812185409657 27.895473256002102 22.730669373729807 56-57 22.08171867449972 27.420452544764977 27.81220014927395 22.685628631461356 58-59 22.242380383040114 27.373710148099267 27.750537221512488 22.63337224734813 60-61 22.154815981578437 27.23961038797563 27.771831758901754 22.833741871544174 62-63 21.99300619485841 27.213669911464617 27.95665680403851 22.836667089638464 64-65 22.09896929226795 27.19176454317062 27.83446304773133 22.874803116830105 66-67 22.04807329495634 27.257065435594996 27.941369003258952 22.753492266189706 68-69 22.123063803213412 27.023607877238366 28.02870951550384 22.824618804044377 70-71 21.925530971846708 26.988281884837566 28.068408165677454 23.017778977638272 72-73 22.06521605869744 26.81001442479432 28.075664587675757 23.049104928832477 74-75 21.911871992132184 26.758183550137176 28.03092645687614 23.299018000854495 76-77 21.925265358263466 26.65203343082479 28.2828963125119 23.139804898399838 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6187.0 1 3753.5 2 1294.5 3 1325.5 4 1382.0 5 1437.5 6 1562.0 7 1715.5 8 1874.0 9 1948.0 10 2031.0 11 2211.5 12 2445.5 13 2582.0 14 2727.5 15 3115.0 16 3666.5 17 3976.0 18 4300.5 19 5148.0 20 6488.5 21 8353.0 22 9400.0 23 11093.0 24 14862.0 25 20172.0 26 23406.0 27 27187.5 28 36202.0 29 45987.5 30 50540.0 31 56762.0 32 68414.5 33 79492.0 34 91359.0 35 97579.0 36 103559.0 37 114194.0 38 121901.0 39 124953.0 40 128234.0 41 134421.0 42 140438.5 43 143550.0 44 143699.0 45 144127.0 46 144786.0 47 145853.0 48 146540.0 49 144931.5 50 139888.0 51 131644.5 52 126836.0 53 122868.5 54 112987.5 55 99416.5 56 84889.5 57 78020.0 58 72952.0 59 60397.5 60 47821.0 61 42731.0 62 37386.5 63 26981.0 64 18929.5 65 15939.0 66 13874.0 67 10377.0 68 7540.5 69 5251.5 70 4367.0 71 3658.0 72 2433.5 73 1698.0 74 1478.0 75 1335.0 76 937.0 77 551.0 78 420.0 79 353.5 80 247.5 81 173.0 82 120.5 83 103.0 84 91.5 85 74.0 86 57.0 87 46.0 88 47.0 89 49.5 90 49.5 91 48.0 92 39.5 93 38.5 94 43.5 95 45.0 96 49.0 97 41.5 98 36.0 99 86.5 100 135.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009061324020529603 2 0.012585172250735561 3 0.013088579140764982 4 0.014464557973512072 5 0.011981083982700255 6 0.009531170451223732 7 0.015404250834900325 8 0.016981592423659184 9 0.016780229667647414 10-11 0.01939794549580041 12-13 0.01552171244257386 14-15 0.0077189056471178115 16-17 0.006074443139688364 18-19 0.017619241151029787 20-21 0.022753991429329892 22-23 0.0204550999648622 24-25 0.018995219983776873 26-27 0.024247431869750515 28-29 0.008457235752494298 30-31 0.00825587299648253 32-33 0.010386962164273749 34-35 0.011460896863003184 36-37 0.011259534106991416 38-39 0.014011491772485592 40-41 0.011242753877323769 42-43 0.014967964863541494 44-45 0.013138919829767927 46-47 0.024230651640082868 48-49 0.02317349717102108 50-51 0.03614461470411253 52-53 0.0392825176519626 54-55 0.027620258032947646 56-57 0.020488660424197495 58-59 0.016075460021606224 60-61 0.019448286184803352 62-63 0.026009355984853492 64-65 0.025942235066182904 66-67 0.027821620788959413 68-69 0.04168209049443618 70-71 0.052790602534418765 72-73 0.08495830280729887 74-75 0.08406895063491356 76-77 0.09007627285593132 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2979697.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.19268921732163 #Duplication Level Percentage of deduplicated Percentage of total 1 75.63136738558352 44.768240247359195 2 13.476414604460357 15.954104428911926 3 4.772591963292925 8.47507658532855 4 2.2323125733218463 5.285463375542382 5 1.1920070695517913 3.527905200641473 6 0.7047835019051524 2.5030818476260372 7 0.4458634345364764 1.8474298999719692 8 0.29806416688146325 1.4114575677627477 9 0.2176336435547097 1.1594088563550555 >10 0.9493552025049534 9.7426338743309 >50 0.05535596716093009 2.2687097985795863 >100 0.023282033130198162 2.4173775104584605 >500 8.545183373962001E-4 0.3635779414038158 >1k 5.6967889135134366E-5 0.06545212243026056 >5k 5.6967889135134366E-5 0.21008074329753904 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6230 0.20908166165888678 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.356045933529483E-5 0.0 2 0.0 0.0 0.0 2.349232153470638E-4 0.0 3 0.0 0.0 0.0 4.02725512023538E-4 0.0 4 0.0 0.0 0.0 9.396928613882552E-4 0.0 5 0.0 0.0 0.0 0.002214990316129459 0.0 6 0.0 0.0 0.0 0.004664903847605982 0.0 7 0.0 0.0 0.0 0.010403742393941397 0.0 8 0.0 0.0 0.0 0.01718295517967095 0.0 9 0.0 0.0 0.0 0.024129970262076982 0.0 10 0.0 0.0 0.0 0.03131190855983008 0.0 11 0.0 0.0 0.0 0.04010474890567732 0.0 12 0.0 0.0 0.0 0.05285772345308936 0.0 13 0.0 3.356045933529483E-5 0.0 0.07151733884351329 0.0 14 0.0 3.356045933529483E-5 0.0 0.0934658792487961 0.0 15 0.0 3.356045933529483E-5 0.0 0.1187369051282731 0.0 16 0.0 3.356045933529483E-5 0.0 0.14424285422309718 0.0 17 0.0 3.356045933529483E-5 0.0 0.16911115459055065 0.0 18 0.0 3.356045933529483E-5 0.0 0.19454998276670413 0.0 19 0.0 3.356045933529483E-5 0.0 0.22237160355566354 0.0 20 0.0 3.356045933529483E-5 0.0 0.2563012279436466 0.0 21 0.0 3.356045933529483E-5 0.0 0.29043221508764144 0.0 22 0.0 3.356045933529483E-5 0.0 0.3277514458684893 0.0 23 0.0 3.356045933529483E-5 0.0 0.3672521065061313 0.0 24 0.0 3.356045933529483E-5 0.0 0.40735685541180866 0.0 25 0.0 3.356045933529483E-5 0.0 0.4513881780597155 0.0 26 0.0 3.356045933529483E-5 0.0 0.49833926066979295 0.0 27 0.0 3.356045933529483E-5 0.0 0.5436794412317763 0.0 28 0.0 3.356045933529483E-5 0.0 0.588381973066389 0.0 29 0.0 3.356045933529483E-5 0.0 0.6334201094943547 0.0 30 0.0 1.006813780058845E-4 0.0 0.6823512592052144 0.0 31 0.0 1.6780229667647415E-4 0.0 0.7416525908506805 0.0 32 0.0 1.6780229667647415E-4 0.0 0.8020614176542111 0.0 33 0.0 1.6780229667647415E-4 0.0 0.8658934113099419 0.0 34 0.0 2.01362756011769E-4 0.0 0.9339875833012551 0.0 35 0.0 2.01362756011769E-4 0.0 1.0054713616854332 0.0 36 0.0 2.01362756011769E-4 0.0 1.0791365699264053 0.0 37 0.0 2.01362756011769E-4 0.0 1.1555537358328716 0.0 38 0.0 2.01362756011769E-4 0.0 1.2322393854140203 0.0 39 0.0 2.01362756011769E-4 0.0 1.3105694975025983 0.0 40 0.0 2.349232153470638E-4 0.0 1.3940007994101413 0.0 41 0.0 2.349232153470638E-4 0.0 1.4832380607826903 0.0 42 0.0 2.349232153470638E-4 0.0 1.5787846885102748 0.0 43 0.0 2.349232153470638E-4 0.0 1.6857754328711947 0.0 44 0.0 2.349232153470638E-4 0.0 1.7951489698449206 0.0 45 0.0 2.349232153470638E-4 0.0 1.9102613453649817 0.0 46 0.0 2.349232153470638E-4 0.0 2.037757530379767 0.0 47 0.0 2.349232153470638E-4 0.0 2.1638441761024696 0.0 48 0.0 2.349232153470638E-4 0.0 2.2911389983612427 0.0 49 0.0 2.349232153470638E-4 0.0 2.4180310951079926 0.0 50 0.0 2.684836746823586E-4 0.0 2.551366800047119 0.0 51 0.0 2.684836746823586E-4 0.0 2.6932604221167455 3.356045933529483E-5 52 0.0 3.020441340176535E-4 0.0 2.842235301106119 3.356045933529483E-5 53 0.0 3.356045933529483E-4 0.0 3.0024529339728168 6.712091867058965E-5 54 0.0 3.356045933529483E-4 0.0 3.1803569289092146 6.712091867058965E-5 55 0.0 3.356045933529483E-4 0.0 3.3590663748696596 6.712091867058965E-5 56 0.0 3.356045933529483E-4 0.0 3.5503945535401753 6.712091867058965E-5 57 0.0 3.356045933529483E-4 0.0 3.740111830162597 6.712091867058965E-5 58 0.0 3.356045933529483E-4 0.0 3.9390246726428897 6.712091867058965E-5 59 0.0 3.356045933529483E-4 0.0 4.1391121311999175 6.712091867058965E-5 60 0.0 3.356045933529483E-4 0.0 4.340307084915009 6.712091867058965E-5 61 0.0 3.356045933529483E-4 0.0 4.557275454517691 6.712091867058965E-5 62 0.0 3.356045933529483E-4 0.0 4.7791772116426605 6.712091867058965E-5 63 0.0 3.356045933529483E-4 0.0 5.010878622893536 6.712091867058965E-5 64 0.0 3.356045933529483E-4 0.0 5.2519769627582935 6.712091867058965E-5 65 0.0 3.356045933529483E-4 0.0 5.505492672577111 6.712091867058965E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAA 330 0.0 21.515717 59 ATTCGCG 315 0.0 21.41254 56 CTCGCGC 345 0.0 20.577139 36 CAATTCG 370 0.0 20.150194 54 CGCGCTT 370 0.0 20.145796 38 TCGCGCT 355 0.0 19.998175 37 CGATCGT 365 0.0 19.447994 1 AATTCGC 405 0.0 18.406345 55 TCGCGGA 370 0.0 18.22929 58 ATCGTTC 405 0.0 17.526615 3 CCTCGCG 420 0.0 16.902369 35 TTGCGCG 295 3.583409E-10 16.843376 5 TCGCGGG 275 2.5465852E-9 16.78564 63 GATCGTT 455 0.0 16.380646 2 ATCGCCC 285 4.105459E-9 16.19232 57 ACGATCG 220 1.2042492E-6 16.136787 59 ACGACGG 155 3.6418508E-4 16.032679 24 CCGCACG 155 3.6451107E-4 16.030794 17 ATCGCGG 230 1.9105119E-6 15.437778 62 GATCGCG 235 2.3895136E-6 15.109315 61 >>END_MODULE