FastQCFastQC Report
Thu 26 May 2016
SRR936115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2979697
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78790.26442285910278795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56510.18965015570375107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38340.12867080109152038No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12050.043.467613100-101
GGTATCA78100.038.783391
CGGTATC9850.036.860714100-101
CGTACAT15850.033.797405100-101
CCTGTCG8850.031.60550796-97
TCGTATC5700.031.32684100-101
GTATCAA154550.031.0345211
TACGCAG11500.030.79564100-101
CGAGTAC9950.030.508245100-101
GCGTATC3450.029.329184100-101
CGAATCG2550.029.17279296-97
ACGTATC4950.027.656218100-101
CCGGTAT4700.027.227688100-101
AGTATCA34400.026.472998100-101
TCGTACA8450.026.41464100-101
ACCGTAC3200.026.04043498-99
TCGGTAT4950.025.85255100-101
AGGTATC25600.024.761663100-101
CCGTATC5800.024.629375100-101
CGAGCCG4800.024.17695296-97
Produced by FastQC (version 0.11.5)