FastQCFastQC Report
Thu 26 May 2016
SRR936114_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936114_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2990401
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64100.2143525232903547No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT2451.1194061E-817.3845521
CGCGGAA2257.99937E-817.35700259
CTCGCGC2652.9462171E-816.07277936
CCTCGCG3107.2577677E-1016.02850235
TCGCGGA2604.1410203E-715.02073558
ATTCGCG3101.2469172E-814.88759156
CGTAGTA2202.1510079E-514.52126638
TCGCGCT3001.3483077E-714.19833537
GCGGTAT1759.158326E-414.1983349
GCCTCGA10100.014.05940221
GTAGACC19250.013.8295468
TTGCGCG1800.001133432513.8034745
CGGTTAC3353.4673576E-813.7740424
CAATTCG3102.0082007E-713.74239254
ACGATCG2658.304114E-613.39734459
CGCGCTT3202.9610965E-713.31116138
TAGACCA20650.013.2357369
AATTCGC3608.8724846E-812.82008455
TCGAACT9450.012.77226924
CGCCCGA2000.002505503412.4229192
Produced by FastQC (version 0.11.5)