##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936114_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2990401 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.532282459777136 39.0 36.0 41.0 30.0 41.0 2 36.40197251137891 39.0 36.0 41.0 30.0 41.0 3 36.26607702445257 39.0 36.0 41.0 29.0 41.0 4 36.165792480674 39.0 36.0 41.0 29.0 41.0 5 36.093731242064194 39.0 35.0 41.0 29.0 41.0 6 36.004174690952816 39.0 35.0 40.0 29.0 41.0 7 35.860376250542984 39.0 35.0 40.0 28.0 41.0 8 35.72809967626415 39.0 35.0 40.0 27.0 41.0 9 35.894311498691984 39.0 35.0 40.0 28.0 41.0 10-11 36.09968144740455 39.0 35.0 41.0 29.0 41.0 12-13 36.0127947723399 39.0 35.0 41.0 28.5 41.0 14-15 35.86396038524599 39.0 35.0 41.0 27.5 41.0 16-17 35.71638786905167 39.0 35.0 41.0 27.0 41.0 18-19 35.56339701598548 39.0 35.0 41.0 27.0 41.0 20-21 35.358513456890904 39.0 35.0 41.0 26.0 41.0 22-23 35.09116168701121 39.0 35.0 41.0 24.5 41.0 24-25 34.87301251571277 39.0 34.0 41.0 24.0 41.0 26-27 33.925610979932124 38.0 33.0 40.0 23.0 40.5 28-29 33.94336395018595 38.0 33.0 39.5 22.5 40.5 30-31 34.367116316507385 38.0 34.0 40.0 23.0 41.0 32-33 34.25405489096613 38.0 34.0 40.0 22.5 41.0 34-35 34.013999125869745 38.0 33.0 40.0 22.0 41.0 36-37 33.718002702647574 38.0 33.0 40.0 20.0 41.0 38-39 33.421816672747234 37.0 33.0 40.0 19.0 41.0 40-41 33.075937641807904 37.0 32.5 40.0 18.0 41.0 42-43 32.715437160434334 36.0 32.0 39.5 15.0 41.0 44-45 32.36141039278679 36.0 32.0 39.0 13.0 41.0 46-47 31.94925613655159 35.5 31.0 39.0 8.0 40.0 48-49 31.530460463329163 35.0 31.0 38.0 7.0 40.0 50-51 31.0720329815299 35.0 31.0 37.0 3.5 39.5 52-53 30.58029441536436 35.0 30.0 37.0 2.0 39.0 54-55 30.10814151680661 34.5 30.0 36.0 2.0 39.0 56-57 29.652916782732483 34.0 29.5 36.0 2.0 37.5 58-59 29.215449198953586 34.0 29.0 35.0 2.0 37.0 60-61 28.841405383425165 34.0 29.0 35.0 2.0 36.5 62-63 28.473928078541974 34.0 29.0 35.0 2.0 36.0 64-65 28.09078531608303 34.0 28.5 35.0 2.0 36.0 66-67 27.728788045482865 34.0 27.0 35.0 2.0 35.5 68-69 27.354324720998953 34.0 26.5 35.0 2.0 35.0 70-71 26.90706764744929 33.0 25.5 35.0 2.0 35.0 72-73 26.436210896130653 33.0 25.0 35.0 2.0 35.0 74-75 25.869548431798947 33.0 23.5 35.0 2.0 35.0 76-77 24.111679503852493 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9339962870584912 1101 2 0.738243378863821 1101 3 1.0053649939660616 1101 4 0.776732062193517 1101 5 0.8364911558015322 1101 6 0.9799756817926237 1101 7 0.788262432679403 1101 8 0.8068239940752235 1101 9 0.7754060129477764 1101 10-11 0.9043267449453154 1101 12-13 1.121278002538233 1101 14-15 1.0698313054003847 1101 16-17 1.0594996705353878 1101 18-19 0.9748025424701439 1101 20-21 1.0527455812604813 1101 22-23 0.980692418548692 1101 24-25 1.0576168659550405 1101 26-27 1.0791887601493926 1101 28-29 1.0365978463950896 1101 30-31 0.9913646564733583 1101 32-33 1.023187172012193 1101 34-35 1.155492473905845 1101 36-37 1.122249448454312 1101 38-39 1.026487730718479 1101 40-41 1.1259519718119861 1101 42-43 1.2011000722238165 1101 44-45 1.195772576472507 1101 46-47 1.1673926148137923 1101 48-49 1.0809651675730372 1101 50-51 0.9724523213899552 1101 52-53 1.0634829608994814 1101 54-55 1.044156563332674 1101 56-57 1.0607851094538283 1101 58-59 1.1592391000714528 1101 60-61 0.9731192353299214 1101 62-63 1.033833026069729 1101 64-65 1.0223295913997497 1101 66-67 1.141435130173928 1101 68-69 0.936290054376677 1101 70-71 0.9876021430665176 1101 72-73 1.0192832803861513 1101 74-75 1.0199016161381884 1101 76-77 1.198216926594025 1102 1 0.9581367519947861 1102 2 0.9543199541433722 1102 3 1.0800072361951578 1102 4 0.9023826274983549 1102 5 0.9266389057348405 1102 6 1.1194255641764101 1102 7 1.0376479254017923 1102 8 1.0153201745080835 1102 9 1.0073156761589317 1102 10-11 1.1514808590141357 1102 12-13 1.3472097190677843 1102 14-15 1.2700523566424806 1102 16-17 1.317478257202005 1102 18-19 1.3935713457910524 1102 20-21 1.4304046359167941 1102 22-23 1.3630527858414823 1102 24-25 1.410048449241593 1102 26-27 1.1926787598602786 1102 28-29 1.3397303061549835 1102 30-31 1.4737402777016584 1102 32-33 1.523095465247259 1102 34-35 1.4305115118900957 1102 36-37 1.5091954810508952 1102 38-39 1.6895448444096175 1102 40-41 1.7007207850754327 1102 42-43 1.5649754431227336 1102 44-45 1.5683946272529354 1102 46-47 1.636442366584113 1102 48-49 1.433637791821642 1102 50-51 1.4175828295357 1102 52-53 1.5142302794577738 1102 54-55 1.5797275373697168 1102 56-57 1.6413354390029333 1102 58-59 1.713838769953263 1102 60-61 1.5906510046595415 1102 62-63 1.5955167491916953 1102 64-65 1.5277016324011754 1102 66-67 1.538417231917677 1102 68-69 1.5837097910068856 1102 70-71 1.4173906065648012 1102 72-73 1.5285035387114156 1102 74-75 1.6732382052143073 1102 76-77 1.6736819655811743 1103 1 0.3764230984771757 1103 2 0.5128664377438596 1103 3 0.629729944186856 1103 4 0.6146291365134928 1103 5 0.45836662807774786 1103 6 0.5635086650463563 1103 7 0.4499143022204066 1103 8 0.5013556047519216 1103 9 0.5990074801353202 1103 10-11 0.7014528600347631 1103 12-13 0.7041404182531039 1103 14-15 0.6868465377965123 1103 16-17 0.6663224704269055 1103 18-19 0.6006414473689787 1103 20-21 0.6173778113565618 1103 22-23 0.4575031385202024 1103 24-25 0.6586623043619397 1103 26-27 0.73025840756479 1103 28-29 0.6502082907125626 1103 30-31 0.6759604164938935 1103 32-33 0.6682188649814123 1103 34-35 0.78670038309631 1103 36-37 0.8675565108082282 1103 38-39 0.6979579641186859 1103 40-41 0.6724372145386752 1103 42-43 0.5926796201014994 1103 44-45 0.5873444902961964 1103 46-47 0.6305383134513605 1103 48-49 0.5669762812029582 1103 50-51 0.6051200816672058 1103 52-53 0.5834455592105705 1103 54-55 0.7162784085275149 1103 56-57 0.6973592410348992 1103 58-59 0.6765618036962593 1103 60-61 0.6023406730991852 1103 62-63 0.5107140052160801 1103 64-65 0.4729600348684109 1103 66-67 0.5086938759531989 1103 68-69 0.4674078170347151 1103 70-71 0.3226213731191194 1103 72-73 0.20636677157619587 1103 74-75 0.22982425599923673 1103 76-77 0.39698737809526463 1104 1 0.6437851676788711 1104 2 0.6249693968494512 1104 3 0.7762994580215192 1104 4 0.8449288778045698 1104 5 0.7250606431345332 1104 6 0.8846280556909534 1104 7 0.6671137243327792 1104 8 0.5812710008681563 1104 9 0.5949970760053702 1104 10-11 0.6906920146123596 1104 12-13 0.8301832896677226 1104 14-15 1.031519851736462 1104 16-17 0.9634295831609805 1104 18-19 0.8230085043495237 1104 20-21 0.8938245363012243 1104 22-23 0.9243461509331397 1104 24-25 0.9097289038073413 1104 26-27 0.7288486689938765 1104 28-29 0.8995259875182242 1104 30-31 1.0557778794439159 1104 32-33 0.9942519761029658 1104 34-35 0.9941171295087301 1104 36-37 0.9096395799531791 1104 38-39 1.0127683282289865 1104 40-41 1.1077118554870893 1104 42-43 1.1625698620589162 1104 44-45 1.0240442808045955 1104 46-47 0.9718788175423683 1104 48-49 0.8636841147799323 1104 50-51 0.807133013688599 1104 52-53 0.7653785840305787 1104 54-55 0.7559595649485651 1104 56-57 0.8396125771471041 1104 58-59 0.7393661095755277 1104 60-61 0.6977835580167664 1104 62-63 0.6618331854884474 1104 64-65 0.643276170516426 1104 66-67 0.7487630223703192 1104 68-69 0.5902454473431895 1104 70-71 0.7677628386601469 1104 72-73 0.7710753130801855 1104 74-75 0.5959914353870595 1104 76-77 0.620679963599347 1105 1 0.13250135493586868 1105 2 0.35724266578488084 1105 3 0.27688861437511747 1105 4 0.38590631375370066 1105 5 0.14943159108347004 1105 6 0.2692995526175608 1105 7 0.3968705825023662 1105 8 0.17151654007461303 1105 9 0.33573479818117846 1105 10-11 0.3763129653912074 1105 12-13 0.5426583075676135 1105 14-15 0.5449813803448933 1105 16-17 0.5220538872874343 1105 18-19 0.47173959299059476 1105 20-21 0.4127315163235252 1105 22-23 0.3902090412797605 1105 24-25 0.3565057258518607 1105 26-27 0.20098584513512208 1105 28-29 0.35023339813881904 1105 30-31 0.6248923123268639 1105 32-33 0.6891671677339772 1105 34-35 0.5891891157288214 1105 36-37 0.6526491495586413 1105 38-39 0.5290041165969228 1105 40-41 0.5823746172428415 1105 42-43 0.7244023247944327 1105 44-45 0.37801542340330485 1105 46-47 0.5163390617911361 1105 48-49 0.5507593982261589 1105 50-51 0.6472806977721071 1105 52-53 0.557289560831439 1105 54-55 0.4598720063889026 1105 56-57 0.5310609866751683 1105 58-59 0.6267991763735488 1105 60-61 0.6833099363620256 1105 62-63 0.4791668338646353 1105 64-65 0.48765935795179516 1105 66-67 0.4913593663241933 1105 68-69 0.47206690264338746 1105 70-71 0.2922980521463643 1105 72-73 -0.12671002143658328 1105 74-75 -0.12229227452614921 1105 76-77 -0.0875950588045562 1106 1 0.16932098825675013 1106 2 0.19819832926301473 1106 3 0.09574054622262906 1106 4 0.3470810238012376 1106 5 0.3220103824364955 1106 6 0.18796161802283962 1106 7 -0.06251889633464458 1106 8 -0.021338765904019397 1106 9 0.10699475847597739 1106 10-11 -0.1234428729005046 1106 12-13 0.1697031469647996 1106 14-15 0.08849977560965527 1106 16-17 0.23447218794103009 1106 18-19 0.34235517869004894 1106 20-21 0.20967252914493884 1106 22-23 0.2564146275851371 1106 24-25 0.06975247064072931 1106 26-27 -0.016609172773257797 1106 28-29 0.2756248202501581 1106 30-31 0.3761446102141619 1106 32-33 0.2492388799480736 1106 34-35 0.13425889802770286 1106 36-37 0.1443953626069714 1106 38-39 0.34077470287606104 1106 40-41 0.2797639715538054 1106 42-43 0.48670750235856275 1106 44-45 0.4641798159189605 1106 46-47 0.47862746889303054 1106 48-49 0.33156754843224334 1106 50-51 0.22263356929032696 1106 52-53 0.1902007818689917 1106 54-55 0.11318355243467337 1106 56-57 0.16091768152480412 1106 58-59 0.06704808295372544 1106 60-61 -0.1608117184317237 1106 62-63 -0.10876263190906954 1106 64-65 0.03643903844944063 1106 66-67 -0.030843127345733024 1106 68-69 0.11191907924899525 1106 70-71 -0.10439339568127792 1106 72-73 -0.13675953604739988 1106 74-75 -0.15450003073372542 1106 76-77 -0.12816943878649667 1107 1 -0.23017989077136036 1107 2 -0.23008086305164 1107 3 -0.11072552885889309 1107 4 -0.19523826518331333 1107 5 -0.1663678918852156 1107 6 -0.37209896301705925 1107 7 -0.21072025175423903 1107 8 -0.3979445577483105 1107 9 -0.4872699890179817 1107 10-11 -0.34293155222456306 1107 12-13 -0.3411715207904109 1107 14-15 -0.37171318576413626 1107 16-17 -0.49830373179140963 1107 18-19 -0.5269879623394544 1107 20-21 -0.4796178238115161 1107 22-23 -0.570927553239521 1107 24-25 -0.5331161993132127 1107 26-27 -0.7143238156651819 1107 28-29 -0.5252689356351539 1107 30-31 -0.3175864582108687 1107 32-33 -0.1974837832055769 1107 34-35 -0.24009609480554417 1107 36-37 -0.19567533073514198 1107 38-39 -0.19525280908011666 1107 40-41 -0.14829987047161808 1107 42-43 -0.17675392013620694 1107 44-45 -0.10016318595042151 1107 46-47 -0.2599718380895588 1107 48-49 -0.31080972393658257 1107 50-51 -0.25766866330190297 1107 52-53 -0.21965859682744693 1107 54-55 -0.22526673725031543 1107 56-57 -0.23411360630935718 1107 58-59 -0.21922759788717272 1107 60-61 -0.2960911287639014 1107 62-63 -0.3989216919288303 1107 64-65 -0.35781817120455983 1107 66-67 -0.43785169497059684 1107 68-69 -0.4477919779504518 1107 70-71 -0.33677966707134743 1107 72-73 -0.4513708850192373 1107 74-75 -0.5001076734076371 1107 76-77 -0.1912249775912933 1108 1 0.22541501162661604 1108 2 0.40239230458777087 1108 3 0.4745150333637653 1108 4 0.2867835067361568 1108 5 0.31456746999644025 1108 6 0.38617261763304356 1108 7 0.29805737300804225 1108 8 0.338166006878879 1108 9 0.2919439485079778 1108 10-11 0.3190632233891435 1108 12-13 0.3732310629855746 1108 14-15 0.4059634924879987 1108 16-17 0.3176421225815531 1108 18-19 0.2646016500941428 1108 20-21 0.29832663156260253 1108 22-23 0.24094863536300437 1108 24-25 0.25102756967710604 1108 26-27 0.24483689432672406 1108 28-29 0.3408938728550197 1108 30-31 0.4659397839470856 1108 32-33 0.4086718116968271 1108 34-35 0.42982379065141885 1108 36-37 0.35706091426452247 1108 38-39 0.4865290563974014 1108 40-41 0.3309900283195546 1108 42-43 0.27704250367300176 1108 44-45 0.3472792692925424 1108 46-47 0.3310707439376799 1108 48-49 0.4253380015973356 1108 50-51 0.32766425470365235 1108 52-53 0.4351619378524241 1108 54-55 0.4760054773211344 1108 56-57 0.504547398783874 1108 58-59 0.43478851245885863 1108 60-61 0.5139772910231883 1108 62-63 0.5000044671635706 1108 64-65 0.46064697405086363 1108 66-67 0.4877559951511614 1108 68-69 0.406948223592817 1108 70-71 0.46336788358771486 1108 72-73 0.5322253070807896 1108 74-75 0.5717221733582711 1108 76-77 0.542349901635756 1109 1 0.01343988430016907 1109 2 -0.08332909742025407 1109 3 -0.01806009568040423 1109 4 0.186314567517897 1109 5 0.16092589071908492 1109 6 0.029087427008391842 1109 7 0.16184598963734942 1109 8 0.06577287091226935 1109 9 0.044799238674855246 1109 10-11 0.10473137588886772 1109 12-13 0.11763213484663737 1109 14-15 0.1686392373369614 1109 16-17 0.21706088702118365 1109 18-19 0.07208406487345798 1109 20-21 0.0842339774181653 1109 22-23 0.14257639553409263 1109 24-25 -0.033027423323162 1109 26-27 -0.030474014092703783 1109 28-29 0.21106362713430116 1109 30-31 0.3861861917524365 1109 32-33 0.2533945277652876 1109 34-35 0.3037961610275133 1109 36-37 0.35969418833630584 1109 38-39 0.3739870794664597 1109 40-41 0.2565145789194716 1109 42-43 0.3584597135705465 1109 44-45 0.24720583650286443 1109 46-47 0.2686694058290655 1109 48-49 0.3034011366191862 1109 50-51 0.29736893464834324 1109 52-53 0.43879149304725473 1109 54-55 0.5844489598389764 1109 56-57 0.5666060505406776 1109 58-59 0.5428083607836456 1109 60-61 0.4672358173859905 1109 62-63 0.35237621744055403 1109 64-65 0.5719414524204005 1109 66-67 0.5775857684972081 1109 68-69 0.4013973917839664 1109 70-71 0.5613871307083755 1109 72-73 0.4789953379139753 1109 74-75 0.4965093900408242 1109 76-77 0.5489567055487363 1110 1 -0.3073657637153815 1110 2 -0.3512341192552313 1110 3 -0.4573899501391523 1110 4 -0.40969676593737603 1110 5 -0.40817691533519707 1110 6 -0.40359436891355926 1110 7 -0.30977633135247373 1110 8 -0.6963602731648919 1110 9 -0.6193394701406447 1110 10-11 -0.553436849977814 1110 12-13 -0.5843438656388642 1110 14-15 -0.4631619105062157 1110 16-17 -0.5249890051314523 1110 18-19 -0.5643479027866647 1110 20-21 -0.7698162830759898 1110 22-23 -0.7656097416760659 1110 24-25 -0.7569206919452682 1110 26-27 -0.9536939847771038 1110 28-29 -0.710485425924567 1110 30-31 -0.4920730405968854 1110 32-33 -0.6349756579816628 1110 34-35 -0.6870894937985597 1110 36-37 -0.6467257639731585 1110 38-39 -0.5580205124145152 1110 40-41 -0.44962275346196634 1110 42-43 -0.4792189468126864 1110 44-45 -0.5723373778794745 1110 46-47 -0.6452345590259263 1110 48-49 -0.5926784074465097 1110 50-51 -0.6633215666079799 1110 52-53 -0.6486418872910598 1110 54-55 -0.5766307022308688 1110 56-57 -0.4907536572810578 1110 58-59 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-0.882078589342953 2314 30-31 -1.0154805263279911 2314 32-33 -1.1475203111108883 2314 34-35 -1.1187113281389145 2314 36-37 -1.7266921661259502 2314 38-39 -1.3154646134820354 2314 40-41 -1.1907585575543393 2314 42-43 -1.2075179805073084 2314 44-45 -1.1433987253522098 2314 46-47 -0.9870528515258918 2314 48-49 -0.9147058582979959 2314 50-51 -0.8037538101842934 2314 52-53 -0.6504010181248923 2314 54-55 -0.9064406474213484 2314 56-57 -0.8689680260901511 2314 58-59 -0.8014388909941701 2314 60-61 -0.7340420904100782 2314 62-63 -0.7829237141289873 2314 64-65 -0.793627822039916 2314 66-67 -0.690659987140215 2314 68-69 -0.7499676623340292 2314 70-71 -0.5553653812613106 2314 72-73 -0.5602924548670067 2314 74-75 -0.4807871403204409 2314 76-77 -0.5026528021845813 2315 1 -1.1771225297566517 2315 2 -1.0364984815283194 2315 3 -0.8846196413092002 2315 4 -0.8811851320981816 2315 5 -0.9455248301069616 2315 6 -0.9456775583347792 2315 7 -1.2236122817914676 2315 8 -1.1533178145161571 2315 9 -0.832824352619923 2315 10-11 -1.0476264471573558 2315 12-13 -0.9956802606149822 2315 14-15 -0.9073476907177564 2315 16-17 -0.8682854317977231 2315 18-19 -0.8074763828606919 2315 20-21 -0.6944029566442325 2315 22-23 -0.9521416631656976 2315 24-25 -0.9989740903967004 2315 26-27 -0.9258748978889457 2315 28-29 -0.8447237220300536 2315 30-31 -0.8662549759113816 2315 32-33 -0.8008408854962283 2315 34-35 -1.0006312118624052 2315 36-37 -1.0044782587341388 2315 38-39 -0.9194498179776929 2315 40-41 -1.0594534011083354 2315 42-43 -1.1337173832522538 2315 44-45 -1.0943198086550368 2315 46-47 -0.9449973949772996 2315 48-49 -0.9013095030745895 2315 50-51 -0.9073720771972766 2315 52-53 -0.9112027376742979 2315 54-55 -1.0052945027579803 2315 56-57 -1.1357921574546843 2315 58-59 -0.977607732928508 2315 60-61 -1.033823822649179 2315 62-63 -1.0278356819584857 2315 64-65 -1.0050542411231582 2315 66-67 -1.0336900239918592 2315 68-69 -1.1056199580097719 2315 70-71 -1.085056943527455 2315 72-73 -1.0367908383227054 2315 74-75 -1.1422860036909732 2315 76-77 -1.1995546298914164 2316 1 -2.226608185462702 2316 2 -1.850194244538244 2316 3 -2.106742307811068 2316 4 -2.885866805736981 2316 5 -2.3192031620301137 2316 6 -2.335461287442243 2316 7 -1.98849400396103 2316 8 -1.7399086920050593 2316 9 -2.03113568200839 2316 10-11 -1.9763128084679735 2316 12-13 -2.587089452196082 2316 14-15 -3.6610642474328543 2316 16-17 -2.8785522328479445 2316 18-19 -2.386479421031865 2316 20-21 -2.1877891860856806 2316 22-23 -2.5726737202793117 2316 24-25 -2.426729642579531 2316 26-27 -2.3254273061144595 2316 28-29 -3.0631011586787764 2316 30-31 -3.1006465646395185 2316 32-33 -3.081916341744474 2316 34-35 -3.0048747454108877 2316 36-37 -2.958806766558588 2316 38-39 -3.0822439902873526 2316 40-41 -2.823396362011991 2316 42-43 -3.0520071646184412 2316 44-45 -2.8878969802930783 2316 46-47 -2.828442088538246 2316 48-49 -2.730020493293008 2316 50-51 -2.800851323934399 2316 52-53 -2.834024056311474 2316 54-55 -2.893313220727908 2316 56-57 -2.7637775037089547 2316 58-59 -2.8656941122336796 2316 60-61 -2.669107005103946 2316 62-63 -2.782146195230382 2316 64-65 -2.7896381807123554 2316 66-67 -2.86249457679542 2316 68-69 -2.8789753422434536 2316 70-71 -2.9723056218821 2316 72-73 -2.8575427834156883 2316 74-75 -3.1588469902286853 2316 76-77 -3.5334345553321675 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76270.0 3 6401.0 4 8442.0 5 10744.0 6 12178.0 7 12773.0 8 14617.0 9 14958.0 10 18189.0 11 20078.0 12 16523.0 13 14058.0 14 13933.0 15 14505.0 16 15097.0 17 15704.0 18 16203.0 19 17431.0 20 18424.0 21 19956.0 22 22119.0 23 25422.0 24 29021.0 25 34108.0 26 39811.0 27 47359.0 28 55232.0 29 65692.0 30 77456.0 31 92869.0 32 113371.0 33 141088.0 34 186335.0 35 248724.0 36 353834.0 37 494295.0 38 474959.0 39 131262.0 40 960.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.219294229695883 26.97856029492762 29.01508313733805 21.787062338038446 2 22.035609011210838 27.035772883026958 28.957788626760873 21.970829479001335 3 22.081180500813865 27.352558633576063 28.570931685826306 21.99532917978377 4 21.790835909833422 27.250485309601437 28.83608643126531 22.12259234929983 5 22.11412785369802 27.374196482919615 28.72851992321019 21.783155740172173 6 22.293638243848143 27.214186526251577 28.70693299513845 21.78524223476183 7 22.091143274358956 27.037418817975002 28.787318312470777 22.08411959519527 8 21.854875662658664 27.323778784888873 28.724618291887555 22.09672726056491 9 21.95029362402078 27.039665163595306 28.82863447940016 22.181406732983756 10-11 22.297112658979355 27.223068625565467 28.600663929559918 21.87915478589526 12-13 22.35913991369971 27.008943428524983 28.51063602062083 22.121280637154477 14-15 22.09732498101264 27.24481438537972 28.601654032393725 22.05620660121392 16-17 22.35967473127674 27.101240065687815 28.580536883012513 21.958548320022928 18-19 22.1967821802874 27.11774135236454 28.439163127412804 22.24631333993526 20-21 22.284444752129083 27.187416233274455 28.337855795900737 22.190283218695722 22-23 22.304469850628163 27.2634750796375 28.07885538057519 22.35319968915915 24-25 22.305821918209137 27.370593239713344 27.97193908908572 22.351645752991804 26-27 22.37010365152432 27.48308051868739 27.841779709866703 22.305036119921585 28-29 22.4735384950331 27.475452281502687 27.81294421960403 22.23806500386018 30-31 22.327579431766686 27.317872544642483 27.94553441388294 22.40901360970789 32-33 22.33571691701179 27.561134548597032 27.753920388427765 22.349228145963412 34-35 22.3225638799463 27.36112160884958 27.9180331377744 22.398281373429715 36-37 22.345054979601077 27.429585342875463 27.983177201901338 22.242182475622126 38-39 22.257303191719746 27.355586299714524 28.100341532190704 22.28676897637503 40-41 22.167698401536974 27.305308293884156 28.000619392089405 22.526373912489465 42-43 22.0814777318626 27.513705572381458 27.866706734447437 22.538109961308503 44-45 22.284586759638753 27.323705674316308 27.96141154529747 22.430296020747473 46-47 22.124717379961737 27.209419776044523 28.037295811203126 22.628567032790613 48-49 22.19460212426703 27.247374348760733 27.888801652858007 22.669221874114225 50-51 22.102056035939697 27.41416932605713 27.768836487333353 22.714938150669823 52-53 22.102733201926448 27.337639041393167 27.874862955216567 22.68476480146382 54-55 22.117099835197386 27.2729598944621 27.90977633739481 22.700163932945706 56-57 22.07599461593737 27.436628649681573 27.794429010257915 22.692947724123147 58-59 22.227779837495774 27.37639476215107 27.801176062743416 22.59464933760974 60-61 22.14824071020468 27.247485271521104 27.752989678859958 22.85128433941426 62-63 22.000003679391845 27.24340464830951 27.947840941228574 22.808750731070074 64-65 22.086299352708867 27.21806963250487 27.86547783397873 22.83015318080754 66-67 22.054410929012196 27.23859417807475 27.912726978389955 22.794267914523108 68-69 22.11513566564375 27.033596455840847 28.0325655080044 22.818702370511012 70-71 21.912516763343085 27.043254109528537 28.042111793095508 23.002117334032864 72-73 22.050055985120608 26.812037235961387 28.077391314241503 23.060515464676502 74-75 21.944829185741273 26.75848247880604 28.012741895457356 23.283946439995333 76-77 21.994375841385676 26.62280235709307 28.2627440907429 23.120077710778357 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6372.0 1 3850.5 2 1278.5 3 1284.5 4 1341.0 5 1412.0 6 1536.5 7 1686.0 8 1813.5 9 1845.0 10 1979.5 11 2231.5 12 2435.0 13 2521.0 14 2683.0 15 3074.5 16 3569.0 17 3834.0 18 4184.5 19 5124.0 20 6499.0 21 8438.0 22 9591.0 23 11175.0 24 14861.5 25 20359.5 26 23755.0 27 27387.5 28 36332.0 29 46122.5 30 50601.0 31 57090.0 32 68613.0 33 79800.5 34 92073.0 35 98192.0 36 103891.5 37 114453.0 38 122392.0 39 125469.0 40 128906.5 41 134931.0 42 140513.0 43 143508.0 44 143900.5 45 144602.0 46 145424.0 47 146327.0 48 146717.0 49 145358.5 50 140458.5 51 131905.0 52 126893.0 53 122896.5 54 113256.5 55 99987.5 56 85417.0 57 78472.0 58 72858.0 59 60389.0 60 48323.0 61 43112.0 62 37862.0 63 27250.0 64 19205.5 65 16523.0 66 14163.0 67 10391.0 68 7626.5 69 5417.0 70 4560.0 71 3785.5 72 2483.0 73 1717.0 74 1479.0 75 1343.5 76 924.0 77 523.5 78 407.0 79 339.5 80 234.0 81 165.5 82 113.5 83 92.0 84 83.0 85 69.0 86 62.0 87 60.0 88 52.0 89 38.0 90 29.0 91 26.0 92 24.5 93 21.5 94 23.0 95 25.5 96 25.0 97 28.0 98 29.5 99 58.0 100 88.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003611555774626881 2 0.008661045792855204 3 0.011637235273797729 4 0.018626264504325675 5 0.014078379454795528 6 0.013677095479836985 7 0.017221770591970775 8 0.0184256225168464 9 0.01748929324194314 10-11 0.02093364736033729 12-13 0.020331721397899478 14-15 0.00849384413662248 16-17 0.006571025089946132 18-19 0.012172280573742452 20-21 0.010918268151997007 22-23 0.01493110790158243 24-25 0.023090548725739457 26-27 0.014396062601637708 28-29 0.008544004633492298 30-31 0.00856072479911557 32-33 0.009881617883354106 34-35 0.011336272292578822 36-37 0.009764576723991197 38-39 0.01646936313892351 40-41 0.012456523389338085 42-43 0.01563335485775988 44-45 0.018158099866874042 46-47 0.01742241257945005 48-49 0.014329181939144616 50-51 0.028006277418981602 52-53 0.03205255749981357 54-55 0.025548413072360526 56-57 0.015566474195266789 58-59 0.01703784877011478 60-61 0.020565803716625294 62-63 0.025966417212942346 64-65 0.02118444984468638 66-67 0.021201170010309654 68-69 0.030731664415575035 70-71 0.058085855375249 72-73 0.07045877793647073 74-75 0.08234681569461753 76-77 0.08020663449483866 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2990401.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.325626958958 #Duplication Level Percentage of deduplicated Percentage of total 1 75.78613399876177 44.96059914272145 2 13.335737946639153 15.823020292894697 3 4.798309024652951 8.539880736910877 4 2.2499229755654633 5.3391236453914175 5 1.1788772891500034 3.4968817143250375 6 0.6948658385165758 2.4734010913414752 7 0.4388116379828227 1.8222942878152781 8 0.30357829795035823 1.4407978285630683 9 0.20733986566581963 1.107051077178979 >10 0.9227429957145069 9.542879962499695 >50 0.05916124527944309 2.37203463979531 >100 0.023445243479203945 2.3967508382903664 >500 9.606258375690835E-4 0.40388956936720616 >1k 5.6507402161571446E-5 0.06651036729137494 >5k 5.6507402161571446E-5 0.2148848056137668 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6410 0.2143525232903547 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.688066249309039E-5 0.0 3 0.0 0.0 0.0 1.6720165623272597E-4 0.0 4 0.0 0.0 0.0 0.001203851924875627 0.0 5 0.0 0.0 0.0 0.002608345837230525 0.0 6 0.0 0.0 0.0 0.004547885049530146 0.0 7 0.0 0.0 0.0 0.009965218711470469 0.0 8 0.0 0.0 0.0 0.015683515354629696 0.0 9 0.0 0.0 0.0 0.022304700941445644 0.0 10 0.0 0.0 0.0 0.030531022428095765 0.0 11 0.0 6.688066249309039E-5 0.0 0.03905830689596479 0.0 12 0.0 6.688066249309039E-5 0.0 0.05156499078217269 0.0 13 0.0 1.0032099373963558E-4 0.0 0.06985685197403291 0.0 14 0.0 1.0032099373963558E-4 0.0 0.09229531424046473 0.0 15 0.0 1.0032099373963558E-4 0.0 0.11546946379432056 0.0 16 0.0 1.0032099373963558E-4 0.0 0.13981402494180548 0.0 17 0.0 1.0032099373963558E-4 0.0 0.16435922807676964 0.0 18 0.0 1.0032099373963558E-4 0.0 0.19134557539273161 0.0 19 0.0 1.0032099373963558E-4 0.0 0.2188669680086383 0.0 20 0.0 1.0032099373963558E-4 0.0 0.2498661550741857 0.0 21 0.0 1.0032099373963558E-4 0.0 0.2829386426770189 0.0 22 0.0 1.0032099373963558E-4 0.0 0.3196895667169721 0.0 23 0.0 1.0032099373963558E-4 0.0 0.3597510835503332 0.0 24 0.0 1.0032099373963558E-4 0.0 0.4001470036961598 0.0 25 0.0 1.0032099373963558E-4 0.0 0.4415795741106293 0.0 26 0.0 1.0032099373963558E-4 0.0 0.4848179224124122 0.0 27 0.0 1.0032099373963558E-4 0.0 0.5270530607767988 0.0 28 0.0 1.0032099373963558E-4 0.0 0.5713614996784712 0.0 29 0.0 1.3376132498618077E-4 0.0 0.6154358562614178 0.0 30 3.344033124654519E-5 1.6720165623272597E-4 0.0 0.6683719006246989 0.0 31 3.344033124654519E-5 2.3408231872581637E-4 0.0 0.722846200225321 0.0 32 3.344033124654519E-5 2.3408231872581637E-4 0.0 0.7828381544816231 0.0 33 3.344033124654519E-5 2.3408231872581637E-4 0.0 0.8463747838500589 0.0 34 3.344033124654519E-5 3.0096298121890674E-4 0.0 0.9117506314370548 0.0 35 3.344033124654519E-5 3.0096298121890674E-4 0.0 0.9815740430798411 0.0 36 3.344033124654519E-5 3.3440331246545194E-4 0.0 1.0547080475160355 0.0 37 3.344033124654519E-5 3.3440331246545194E-4 0.0 1.1305507187832 0.0 38 3.344033124654519E-5 3.3440331246545194E-4 0.0 1.2081657276064315 0.0 39 3.344033124654519E-5 3.3440331246545194E-4 0.0 1.2880546789544278 0.0 40 3.344033124654519E-5 3.6784364371199714E-4 0.0 1.370685737464641 0.0 41 3.344033124654519E-5 3.6784364371199714E-4 0.0 1.4597373395741908 0.0 42 3.344033124654519E-5 3.6784364371199714E-4 0.0 1.5531696250770382 0.0 43 3.344033124654519E-5 3.6784364371199714E-4 0.0 1.6546275900790564 0.0 44 3.344033124654519E-5 4.0128397495854234E-4 0.0 1.7693613665859529 0.0 45 3.344033124654519E-5 4.0128397495854234E-4 0.0 1.8877735795299695 0.0 46 3.344033124654519E-5 4.0128397495854234E-4 0.0 2.015582525554265 0.0 47 3.344033124654519E-5 4.3472430620508754E-4 0.0 2.1401143191163996 0.0 48 3.344033124654519E-5 4.3472430620508754E-4 0.0 2.2656158822846835 0.0 49 3.344033124654519E-5 4.3472430620508754E-4 0.0 2.394060194602664 0.0 50 3.344033124654519E-5 4.3472430620508754E-4 0.0 2.5263501450139962 0.0 51 3.344033124654519E-5 4.3472430620508754E-4 0.0 2.6674349025431705 0.0 52 3.344033124654519E-5 4.6816463745163274E-4 0.0 2.814806442346695 0.0 53 3.344033124654519E-5 4.6816463745163274E-4 0.0 2.9746177853739346 0.0 54 3.344033124654519E-5 4.6816463745163274E-4 0.0 3.1480393432185183 0.0 55 3.344033124654519E-5 4.6816463745163274E-4 0.0 3.3270454363812747 0.0 56 3.344033124654519E-5 4.6816463745163274E-4 0.0 3.516117069249241 0.0 57 3.344033124654519E-5 4.6816463745163274E-4 0.0 3.707596405966959 0.0 58 3.344033124654519E-5 4.6816463745163274E-4 0.0 3.9048274796590827 0.0 59 3.344033124654519E-5 4.6816463745163274E-4 0.0 4.100654059438851 0.0 60 3.344033124654519E-5 4.6816463745163274E-4 0.0 4.304506318717791 0.0 61 3.344033124654519E-5 4.6816463745163274E-4 0.0 4.5177218707457625 0.0 62 3.344033124654519E-5 5.016049686981779E-4 0.0 4.734114254242157 0.0 63 3.344033124654519E-5 5.016049686981779E-4 0.0 4.959234564193899 0.0 64 3.344033124654519E-5 5.016049686981779E-4 0.0 5.195992109419439 0.0 65 3.344033124654519E-5 5.016049686981779E-4 0.0 5.446861474431021 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATCGT 245 1.1194061E-8 17.384552 1 CGCGGAA 225 7.99937E-8 17.357002 59 CTCGCGC 265 2.9462171E-8 16.072779 36 CCTCGCG 310 7.2577677E-10 16.028502 35 TCGCGGA 260 4.1410203E-7 15.020735 58 ATTCGCG 310 1.2469172E-8 14.887591 56 CGTAGTA 220 2.1510079E-5 14.521266 38 TCGCGCT 300 1.3483077E-7 14.198335 37 GCGGTAT 175 9.158326E-4 14.198334 9 GCCTCGA 1010 0.0 14.059402 21 GTAGACC 1925 0.0 13.829546 8 TTGCGCG 180 0.0011334325 13.803474 5 CGGTTAC 335 3.4673576E-8 13.774042 4 CAATTCG 310 2.0082007E-7 13.742392 54 ACGATCG 265 8.304114E-6 13.397344 59 CGCGCTT 320 2.9610965E-7 13.311161 38 TAGACCA 2065 0.0 13.235736 9 AATTCGC 360 8.8724846E-8 12.820084 55 TCGAACT 945 0.0 12.772269 24 CGCCCGA 200 0.0025055034 12.422919 2 >>END_MODULE