FastQCFastQC Report
Thu 26 May 2016
SRR936114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2990401
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76590.25611949701728964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57940.19375327924248287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39320.13148738246141572No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12250.044.703274100-101
GGTATCA82000.039.0420381
CGGTATC10300.038.141193100-101
CGTACAT15800.032.77553100-101
ACGTATC4700.032.294598100-101
CCGTATC5400.030.312832100-101
GTATCAA155650.030.1259781
CCTGTCG8350.029.93564696-97
CCGGTAT4100.029.76169100-101
TACGCAG8850.029.593544100-101
CCGTACA7750.028.417612100-101
TCGTACA8800.027.394281100-101
AGTATCA33950.027.175621100-101
TCCGGTA3450.026.74037498-99
CGAGTAC10500.025.51002100-101
GCGTATC4350.025.314539100-101
TCGGTAT5000.024.999819100-101
TGTCTCG13100.024.98717796-97
CACGTAT3900.024.41800998-99
TCGAGTA5900.024.2129100-101
Produced by FastQC (version 0.11.5)