FastQCFastQC Report
Thu 26 May 2016
SRR936113_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936113_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3435963
Sequences flagged as poor quality0
Sequence length77
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47600.13853466990185867No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG750.001687123423.66999252
CGATCGT2850.023.6617221
ATTCGCG3100.021.75795456
CTCGCGC3350.020.13158836
TCGCGCT3750.018.93213537
CGCGCTT4000.018.63550438
CGCGGAA3450.018.52218459
AACGCGA1000.00893113317.75094253
CGCTCCG4400.017.7478425
TCGCGGA3850.017.51913558
CAATTCG4500.017.3557254
TATAGCG1905.3548247E-616.81594821
CCTCGCG4250.016.70360835
AATTCGC4500.016.56441355
AACTCGA4550.016.38214713
TCGAACT10300.016.1987124
CGCCGTA1553.6255943E-416.0422271
CGATTCG2051.1041802E-515.5821095
GCCTCGA10950.015.5617921
CGAACTC10550.015.47679125
Produced by FastQC (version 0.11.5)