##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936113_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3435963 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.28149837469146 39.0 36.0 41.0 29.0 41.0 2 36.13482595708976 39.0 36.0 41.0 28.0 41.0 3 36.04168904030689 39.0 36.0 41.0 28.0 41.0 4 35.923389163387384 39.0 35.0 41.0 27.0 41.0 5 35.843512866698504 39.0 35.0 41.0 27.0 41.0 6 35.75195483769761 39.0 35.0 40.0 27.0 41.0 7 35.58619810516004 39.0 35.0 40.0 27.0 41.0 8 35.41277860093371 38.0 35.0 40.0 27.0 41.0 9 35.32651603058589 38.0 34.0 40.0 26.0 41.0 10-11 35.544285692249886 39.0 35.0 40.0 27.0 41.0 12-13 35.729807043905886 39.0 35.0 41.0 27.0 41.0 14-15 35.67602124935571 39.0 35.0 41.0 27.0 41.0 16-17 35.51272059681667 39.0 35.0 41.0 26.5 41.0 18-19 35.368325852170116 39.0 35.0 41.0 26.0 41.0 20-21 35.1830760401087 39.0 35.0 41.0 25.5 41.0 22-23 34.94937852939627 39.0 34.0 41.0 24.0 41.0 24-25 34.73360510575929 39.0 34.0 41.0 24.0 41.0 26-27 33.80676378063443 38.0 33.0 39.5 22.5 40.5 28-29 33.83911337229184 38.0 33.0 39.5 22.5 40.5 30-31 34.21361813267489 38.0 33.0 40.0 22.5 41.0 32-33 34.05042181769711 38.0 33.0 40.0 22.0 41.0 34-35 33.77481291271181 38.0 33.0 40.0 20.5 41.0 36-37 33.479645735416824 37.5 33.0 40.0 19.5 41.0 38-39 33.19257657896782 37.0 32.5 40.0 18.0 41.0 40-41 32.812287122998704 36.5 32.0 40.0 16.0 41.0 42-43 32.44509239476676 36.0 32.0 39.0 13.0 41.0 44-45 32.06732945028803 35.5 31.0 39.0 9.5 40.5 46-47 31.67068795560371 35.0 31.0 38.5 7.5 40.0 48-49 31.201383571359763 35.0 31.0 37.5 6.0 40.0 50-51 30.75192282338314 35.0 30.0 37.0 2.0 39.0 52-53 30.292276139178448 35.0 30.0 36.5 2.0 39.0 54-55 29.822040865981386 34.0 29.5 36.0 2.0 38.5 56-57 29.359908561297082 34.0 29.0 35.5 2.0 37.0 58-59 28.91873544621988 34.0 29.0 35.0 2.0 37.0 60-61 28.557602482913815 34.0 29.0 35.0 2.0 36.5 62-63 28.198693932385186 34.0 28.0 35.0 2.0 36.0 64-65 27.81093379061416 34.0 27.0 35.0 2.0 36.0 66-67 27.411067726864346 34.0 26.5 35.0 2.0 35.0 68-69 27.034026268618142 33.0 26.0 35.0 2.0 35.0 70-71 26.586787314065955 33.0 25.0 35.0 2.0 35.0 72-73 26.082332958765853 33.0 24.5 35.0 2.0 35.0 74-75 25.46976728212731 33.0 21.5 35.0 2.0 35.0 76-77 23.74817001812883 31.0 9.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0195371621876248 1101 2 0.9969854703183927 1101 3 1.1193252707562067 1101 4 0.9838958476163597 1101 5 0.9812092524551232 1101 6 0.9256780387222037 1101 7 1.1310065914575063 1101 8 1.1985050255177256 1101 9 1.1620776675414035 1101 10-11 1.136818612903113 1101 12-13 1.192022113731639 1101 14-15 1.0845887209824525 1101 16-17 1.2216123373945038 1101 18-19 1.1608348158203512 1101 20-21 1.1851113338358985 1101 22-23 1.1446899723830484 1101 24-25 1.2957349236219713 1101 26-27 1.13720325773496 1101 28-29 1.1687358012190572 1101 30-31 1.2637799829911671 1101 32-33 1.359595408951641 1101 34-35 1.4007664656729446 1101 36-37 1.374049809333485 1101 38-39 1.4525273813419588 1101 40-41 1.2526595650385346 1101 42-43 1.1840532505820747 1101 44-45 1.254149951974128 1101 46-47 1.1685415088029636 1101 48-49 1.3372203710231645 1101 50-51 1.168774936106054 1101 52-53 1.4080132723016625 1101 54-55 1.333303827783837 1101 56-57 1.2451450312542498 1101 58-59 1.2718520402544016 1101 60-61 1.143308913780107 1101 62-63 1.0288401591773564 1101 64-65 0.9946576467494808 1101 66-67 1.0439556917906643 1101 68-69 1.1745738838567625 1101 70-71 1.1962924895322686 1101 72-73 1.2363385707773027 1101 74-75 1.2431595097382306 1101 76-77 1.3656953687735154 1102 1 0.8535706423191058 1102 2 0.8317742889268374 1102 3 0.772203845483169 1102 4 0.7723932639233482 1102 5 0.9202400527312093 1102 6 0.725453594731178 1102 7 0.7032391892380616 1102 8 0.8054473642065432 1102 9 0.7997978281569971 1102 10-11 0.7725347395797328 1102 12-13 0.8614772293266384 1102 14-15 0.7362260686968654 1102 16-17 0.7398875844146886 1102 18-19 0.7995540401950265 1102 20-21 0.7952571003965758 1102 22-23 0.8688889689749004 1102 24-25 0.8987041448237747 1102 26-27 0.7645057123653487 1102 28-29 0.8090387273868487 1102 30-31 0.9140690559173947 1102 32-33 0.8824598473193603 1102 34-35 0.8391533196398626 1102 36-37 0.8632498456506497 1102 38-39 0.870733655034762 1102 40-41 0.9852159030185703 1102 42-43 0.8977939284439387 1102 44-45 0.8849781005113329 1102 46-47 0.7759652940084116 1102 48-49 0.8572158997161665 1102 50-51 0.8842327227700366 1102 52-53 0.8606287916420179 1102 54-55 0.8556468414642815 1102 56-57 1.0192027618804609 1102 58-59 0.9551250076890305 1102 60-61 0.8944717464800078 1102 62-63 0.9534290771698757 1102 64-65 0.9534827669036545 1102 66-67 0.8659567676378899 1102 68-69 0.7104274690541992 1102 70-71 0.8619966374017096 1102 72-73 0.7970303151630311 1102 74-75 0.7631465937138664 1102 76-77 0.9287639838105761 1103 1 0.7913005029622795 1103 2 0.4397235853066874 1103 3 0.4717311846173402 1103 4 0.6103653144316539 1103 5 0.6036021949396329 1103 6 0.5629764221025368 1103 7 0.6627143794254664 1103 8 0.8089952504163662 1103 9 0.5924637721603574 1103 10-11 0.5792594146253904 1103 12-13 0.6212991025871233 1103 14-15 0.7457476460560173 1103 16-17 0.789930065298293 1103 18-19 0.8879675012639794 1103 20-21 0.8998845869931174 1103 22-23 0.8715286431904659 1103 24-25 0.7689728974483145 1103 26-27 0.5977965648150771 1103 28-29 0.6186032739156246 1103 30-31 0.8471269959363426 1103 32-33 0.7399608971343028 1103 34-35 0.8404038201330337 1103 36-37 0.8375796730903744 1103 38-39 0.7041529267073301 1103 40-41 0.9003654650260131 1103 42-43 0.9067031951359965 1103 44-45 0.8584793065778058 1103 46-47 0.6339350984010217 1103 48-49 0.6246374565673101 1103 50-51 0.7021054559021955 1103 52-53 0.7898756592872083 1103 54-55 0.859231349125487 1103 56-57 0.9085962129883818 1103 58-59 0.7892254383573025 1103 60-61 0.8542970377426187 1103 62-63 0.9060384776523023 1103 64-65 0.9045916644514804 1103 66-67 0.9838412196344919 1103 68-69 0.7793339036873483 1103 70-71 0.8876824351145771 1103 72-73 0.9197995804878865 1103 74-75 0.9932523564654545 1103 76-77 1.1358509670818968 1104 1 0.8555645433277874 1104 2 0.8258800189931605 1104 3 0.5923838297025625 1104 4 0.648323573351199 1104 5 0.7940506427979557 1104 6 0.6595247780634494 1104 7 0.8010789521024506 1104 8 0.9562374608094473 1104 9 0.9257121437264857 1104 10-11 0.8644301184209269 1104 12-13 0.970618252507073 1104 14-15 1.000679185366728 1104 16-17 0.8512048385890907 1104 18-19 0.8571223339702172 1104 20-21 0.8442921376089529 1104 22-23 1.0621299644326427 1104 24-25 0.8833479082852733 1104 26-27 0.7534358298668948 1104 28-29 0.7626703994914266 1104 30-31 0.9808455470781254 1104 32-33 1.007745071126756 1104 34-35 1.0860089270866027 1104 36-37 1.002710959249697 1104 38-39 1.0871860039994203 1104 40-41 0.9695344580267857 1104 42-43 1.0802358707380932 1104 44-45 0.9994696024412661 1104 46-47 1.0239809893147402 1104 48-49 1.137312076117766 1104 50-51 0.9751162235057542 1104 52-53 1.0323484512927088 1104 54-55 1.1174012611004756 1104 56-57 0.9731596424383255 1104 58-59 0.8906900173919752 1104 60-61 0.83447985003491 1104 62-63 0.9391518929419505 1104 64-65 0.9937521034784105 1104 66-67 0.9354991300373996 1104 68-69 0.826728409493068 1104 70-71 0.7394836823764415 1104 72-73 0.6721993169330212 1104 74-75 0.595709023074324 1104 76-77 0.6337572663900666 1105 1 0.44794566991414797 1105 2 0.683019089061986 1105 3 0.6198567795795 1105 4 0.7177646883263833 1105 5 0.6785534056578229 1105 6 0.44067204184565156 1105 7 0.5183274837591725 1105 8 0.7790165975156214 1105 9 0.619007621467766 1105 10-11 0.6590404303962956 1105 12-13 0.8375051564642249 1105 14-15 0.477697042556926 1105 16-17 0.557958062619889 1105 18-19 0.7330100714623669 1105 20-21 0.7349527775259972 1105 22-23 0.669378480941063 1105 24-25 0.7003080163835165 1105 26-27 0.584004125770683 1105 28-29 0.8195005579312422 1105 30-31 0.6743999660065256 1105 32-33 0.850860890075829 1105 34-35 0.942874570410126 1105 36-37 1.0160591644227637 1105 38-39 0.9026222881829753 1105 40-41 0.9921033363825558 1105 42-43 0.8290784462362524 1105 44-45 0.7626167139830926 1105 46-47 0.6660190827350441 1105 48-49 0.6850506059226049 1105 50-51 0.8045669538619151 1105 52-53 0.646285035223169 1105 54-55 0.6410184962258896 1105 56-57 0.6375803848487429 1105 58-59 0.5804027337427797 1105 60-61 0.3166238906384038 1105 62-63 0.42523846696921197 1105 64-65 0.610594319961951 1105 66-67 0.5640751220330387 1105 68-69 0.400880833921061 1105 70-71 0.23138284698578104 1105 72-73 0.21519917567515634 1105 74-75 0.3212697088528387 1105 76-77 0.6495377198618861 1106 1 0.22724783278336957 1106 2 0.1618480209593116 1106 3 -0.017573681674264208 1106 4 0.20018413693653514 1106 5 0.055741692671666954 1106 6 0.1401691465510737 1106 7 0.3607319906200388 1106 8 0.35857640541929214 1106 9 0.11543099219263553 1106 10-11 0.03266890421601687 1106 12-13 0.18990115514387895 1106 14-15 0.1740350386053322 1106 16-17 0.34796309421805915 1106 18-19 0.23461525141993889 1106 20-21 0.05809366057430765 1106 22-23 0.10101086138203641 1106 24-25 0.1191484134470926 1106 26-27 0.1513546086094948 1106 28-29 0.23762268160199085 1106 30-31 0.15923899359869154 1106 32-33 0.19338235372485002 1106 34-35 0.24816626989806423 1106 36-37 0.2657727714475797 1106 38-39 0.29716174768389436 1106 40-41 0.23751665581460202 1106 42-43 0.16903021431490828 1106 44-45 -0.12518261462070512 1106 46-47 0.011212697234508795 1106 48-49 -0.06350944628660216 1106 50-51 -0.13737410936409233 1106 52-53 -0.2167469457508524 1106 54-55 0.09577827850865361 1106 56-57 0.0444820257014662 1106 58-59 -0.19792834768427525 1106 60-61 -0.16513179513366083 1106 62-63 -0.3325291926083125 1106 64-65 -0.27536577339941815 1106 66-67 -0.3042320068705351 1106 68-69 -0.23761059923038985 1106 70-71 -0.39391169423090844 1106 72-73 -0.5155266668435665 1106 74-75 -0.6406544797192915 1106 76-77 -0.333929474514818 1107 1 0.2149313217602895 1107 2 0.12205577877565332 1107 3 0.25170884291133433 1107 4 0.18625513301007857 1107 5 0.25721982921538 1107 6 0.14375799912447462 1107 7 0.010896655673555244 1107 8 0.19203723952068685 1107 9 0.16026592961107156 1107 10-11 0.29348006539591154 1107 12-13 0.3585445123779252 1107 14-15 0.33024795083989034 1107 16-17 0.2725393433021992 1107 18-19 0.21568962219530619 1107 20-21 0.2173413227167984 1107 22-23 0.24789449864910296 1107 24-25 0.015965878763097408 1107 26-27 0.09305605935676198 1107 28-29 0.2670060059454471 1107 30-31 0.3298421054717551 1107 32-33 0.47558571319336096 1107 34-35 0.4427176718996435 1107 36-37 0.49524915616051857 1107 38-39 0.3814995575470874 1107 40-41 0.4615582770468052 1107 42-43 0.5199950523495289 1107 44-45 0.4929263539099651 1107 46-47 0.34534996073803015 1107 48-49 0.46572587669343335 1107 50-51 0.5048025882002669 1107 52-53 0.6676276207924126 1107 54-55 0.5408828130448171 1107 56-57 0.47003973138793853 1107 58-59 0.5213780872435443 1107 60-61 0.4762862149537135 1107 62-63 0.6385876644852715 1107 64-65 0.5407983489222623 1107 66-67 0.5801448609134354 1107 68-69 0.5013741060018866 1107 70-71 0.5460325110599804 1107 72-73 0.6175181661084679 1107 74-75 0.5315502956890903 1107 76-77 0.49512213726064047 1108 1 0.368367389105309 1108 2 0.30966416785759066 1108 3 0.5588562608745491 1108 4 0.3575794446663281 1108 5 0.4923899490250321 1108 6 0.37138998343138496 1108 7 0.34813815290275585 1108 8 0.43710789810265993 1108 9 0.337340009873472 1108 10-11 0.2426620021361785 1108 12-13 0.3468681889084735 1108 14-15 0.45114259521186284 1108 16-17 0.4341623131621901 1108 18-19 0.4206503470926677 1108 20-21 0.24240091235238737 1108 22-23 0.26877336655278583 1108 24-25 0.37796872089980127 1108 26-27 0.24291348651471623 1108 28-29 0.3211183177928447 1108 30-31 0.2814269457600034 1108 32-33 0.3269924793495065 1108 34-35 0.42282504508547447 1108 36-37 0.4281234185555505 1108 38-39 0.4074699798040129 1108 40-41 0.45811689721756466 1108 42-43 0.39922601815069214 1108 44-45 0.30883360928913106 1108 46-47 0.2829288642616241 1108 48-49 0.22113359587704196 1108 50-51 0.3265607357887035 1108 52-53 0.28676147743227176 1108 54-55 0.4063658830811505 1108 56-57 0.2560173657446505 1108 58-59 0.2541131913597603 1108 60-61 0.2790207971416585 1108 62-63 0.3065368117256462 1108 64-65 0.3185118082662548 1108 66-67 0.3551251022002049 1108 68-69 0.44763328677106173 1108 70-71 0.34151966162645664 1108 72-73 0.3809585817260519 1108 74-75 0.22973912832194188 1108 76-77 0.3038261893558598 1109 1 0.2667065850929973 1109 2 0.32413948010466953 1109 3 -0.05548694783560393 1109 4 0.32853389444338177 1109 5 0.2764918878873459 1109 6 0.18716265991312753 1109 7 0.14472532314783138 1109 8 0.2944340970180619 1109 9 0.37617574494833406 1109 10-11 0.2171885277614649 1109 12-13 0.3484811079728303 1109 14-15 0.4685724492329797 1109 16-17 0.619743232780607 1109 18-19 0.36211941836069883 1109 20-21 0.37893658495888616 1109 22-23 0.4088516552985908 1109 24-25 0.2294928215258949 1109 26-27 0.06593165781448818 1109 28-29 0.22379459452666595 1109 30-31 0.33628234510445054 1109 32-33 0.3614006441336741 1109 34-35 0.4782692940506763 1109 36-37 0.47158441407319174 1109 38-39 0.47568049027012904 1109 40-41 0.4447015745855154 1109 42-43 0.414097572096928 1109 44-45 0.34309473394711176 1109 46-47 0.21096189634790008 1109 48-49 0.46462317248072793 1109 50-51 0.3522042495360509 1109 52-53 0.40257293391683646 1109 54-55 0.4098556526373187 1109 56-57 0.49102650859115826 1109 58-59 0.5007166247423989 1109 60-61 0.4190075626689058 1109 62-63 0.516171842110495 1109 64-65 0.6226665919889207 1109 66-67 0.5563116996520563 1109 68-69 0.5880413605919266 1109 70-71 0.7119088295498983 1109 72-73 0.6593847028058022 1109 74-75 0.6431589818916841 1109 76-77 0.5263199519946795 1110 1 -0.24670908206044118 1110 2 -0.3105274278826542 1110 3 -0.3652054039867423 1110 4 -0.19051682898351885 1110 5 -0.15403241071763318 1110 6 -0.2824386478695402 1110 7 -0.4316671976983031 1110 8 -0.3572364797340839 1110 9 -0.3166777512562149 1110 10-11 -0.2547148475847436 1110 12-13 -0.21343685025224346 1110 14-15 -0.24788720324337987 1110 16-17 -0.2655994193721085 1110 18-19 -0.3249976146558211 1110 20-21 -0.4909275887576001 1110 22-23 -0.5386136226284037 1110 24-25 -0.5037433768750716 1110 26-27 -0.427102778322201 1110 28-29 -0.3471947114464342 1110 30-31 -0.27460592638330894 1110 32-33 -0.05255619974610681 1110 34-35 0.05717941955005301 1110 36-37 -0.01940848643166504 1110 38-39 -0.006618041743656988 1110 40-41 0.027318508301625855 1110 42-43 0.044180651618063393 1110 44-45 0.093957661710931 1110 46-47 -0.09611374478436474 1110 48-49 -0.0447894282826482 1110 50-51 -0.07353959988390812 1110 52-53 -0.11096870194226582 1110 54-55 -0.005402671261002467 1110 56-57 0.004369375101322959 1110 58-59 -0.027784490059580946 1110 60-61 -0.060702754808463766 1110 62-63 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-0.6764693720737327 2314 46-47 -0.651386171468129 2314 48-49 -0.6736771566675159 2314 50-51 -0.5878379330405039 2314 52-53 -0.5820965891973451 2314 54-55 -0.5543167009252521 2314 56-57 -0.42461506792251313 2314 58-59 -0.40115854133761175 2314 60-61 -0.41672136797843606 2314 62-63 -0.29025287011003087 2314 64-65 -0.3563143436252467 2314 66-67 -0.4676883653641539 2314 68-69 -0.3420425301781904 2314 70-71 -0.2344409919386763 2314 72-73 -0.14926317402878198 2314 74-75 -0.17657029766618138 2314 76-77 0.02425101701243193 2315 1 -1.4024931744266596 2315 2 -1.4303016817216587 2315 3 -1.357594231608303 2315 4 -1.365012385715012 2315 5 -1.2947526794451107 2315 6 -1.2975617640191075 2315 7 -1.3361121356073866 2315 8 -1.3067573257288387 2315 9 -1.2112440099894428 2315 10-11 -1.3805317566287343 2315 12-13 -1.438331005205157 2315 14-15 -1.7603195259512177 2315 16-17 -1.5860392820149656 2315 18-19 -1.3423988152716007 2315 20-21 -1.3937098178710627 2315 22-23 -1.2618871995685623 2315 24-25 -1.4999132173940453 2315 26-27 -1.413274886923631 2315 28-29 -1.3871872581475557 2315 30-31 -1.5204820027330612 2315 32-33 -1.556359087550426 2315 34-35 -1.4716319555742245 2315 36-37 -1.7010485028896554 2315 38-39 -1.5671492191705951 2315 40-41 -1.5857933612675126 2315 42-43 -1.6329848670237475 2315 44-45 -1.678502398954521 2315 46-47 -1.6177861289088824 2315 48-49 -1.54765266650859 2315 50-51 -1.343397266196881 2315 52-53 -1.3480295505936688 2315 54-55 -1.3513784295467346 2315 56-57 -1.5083133937035065 2315 58-59 -1.4236222997136387 2315 60-61 -1.4720174568614404 2315 62-63 -1.454628575060383 2315 64-65 -1.5406950933790036 2315 66-67 -1.481859382692221 2315 68-69 -1.4890410166115515 2315 70-71 -1.488947304035392 2315 72-73 -2.8077077804920343 2315 74-75 -3.4399724409790444 2315 76-77 -3.206807197079428 2316 1 -3.401126667794827 2316 2 -3.316159676101975 2316 3 -2.7291539741905666 2316 4 -2.8094382385587338 2316 5 -3.3919208253669524 2316 6 -2.9602062665563054 2316 7 -3.603684003813882 2316 8 -3.296646644158834 2316 9 -2.882134023326479 2316 10-11 -2.848013272533187 2316 12-13 -2.876347239844911 2316 14-15 -2.9804616150172905 2316 16-17 -4.23806760487194 2316 18-19 -4.011006138124259 2316 20-21 -3.4189009892426157 2316 22-23 -3.3968200899645993 2316 24-25 -3.4895208323090827 2316 26-27 -3.179777058687961 2316 28-29 -3.371290648229234 2316 30-31 -3.5603015427132405 2316 32-33 -3.5817519689975583 2316 34-35 -3.644206507013898 2316 36-37 -3.4745182327103734 2316 38-39 -3.5093825899588893 2316 40-41 -3.512599508101516 2316 42-43 -3.8234019119567506 2316 44-45 -3.736846031203388 2316 46-47 -3.26386127094759 2316 48-49 -3.4655335741176074 2316 50-51 -3.7906072072520764 2316 52-53 -4.6494274215596505 2316 54-55 -3.982708234152689 2316 56-57 -3.8413636327486493 2316 58-59 -3.9341496377592975 2316 60-61 -3.7747446432648566 2316 62-63 -3.7754958564488135 2316 64-65 -3.7895731710042924 2316 66-67 -3.832498035349488 2316 68-69 -3.8954580442797884 2316 70-71 -3.730588637048367 2316 72-73 -3.600131495381607 2316 74-75 -3.520884150378418 2316 76-77 -3.441848855533337 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 100737.0 3 8137.0 4 9800.0 5 11623.0 6 13442.0 7 14628.0 8 16060.0 9 16269.0 10 18722.0 11 20400.0 12 17866.0 13 16572.0 14 16487.0 15 17467.0 16 18050.0 17 19059.0 18 19853.0 19 21350.0 20 22611.0 21 24547.0 22 27245.0 23 31017.0 24 35816.0 25 41778.0 26 48912.0 27 56780.0 28 66850.0 29 79334.0 30 93229.0 31 111046.0 32 136096.0 33 168896.0 34 222510.0 35 296170.0 36 417484.0 37 557128.0 38 491020.0 39 129885.0 40 1087.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.731851713865332 26.546932098828318 28.28700786312945 22.434208324176904 2 22.45520328800298 26.770561661698956 28.174337509314455 22.59989754098361 3 22.6363759676271 26.962222883300257 27.685821952538607 22.715579196534037 4 22.468933192719724 26.982298229124307 27.91423695333815 22.634531624817818 5 22.639506496976733 26.94150319558518 27.885115532695043 22.533874774743047 6 22.86755143455578 26.9153703485942 27.787117813686464 22.429960403163555 7 22.650045977218937 26.66821330984454 27.94119573370996 22.740544979226566 8 22.524825824796075 26.974349510120415 27.678211201589132 22.822613463494378 9 22.32053684304595 26.799943993947622 28.020608131415116 22.858911031591315 10-11 22.834209592498006 26.923507974826848 27.73569120357284 22.506591229102305 12-13 22.914035960982595 26.65965145150914 27.71749350146563 22.708819086042634 14-15 22.586532133729218 26.8911999892889 27.80616930112126 22.71609857586062 16-17 22.93927277987485 26.69977160676547 27.82894577909103 22.53200983426865 18-19 22.748851724516346 26.787922927638924 27.643566091444615 22.81965925640011 20-21 22.760131013957967 26.871688790761194 27.69436131423249 22.673818881048344 22-23 22.841385365160704 26.975994983817923 27.35017096121949 22.832448689801886 24-25 22.76696970287974 27.040021913386813 27.31258567194018 22.880422711793262 26-27 22.86654809890583 27.134026836836 27.24461827396139 22.754806790296772 28-29 22.994373016675752 27.00924344616164 27.264753458379026 22.73163007878359 30-31 22.771367581072933 26.902451689218292 27.39449516717545 22.93168556253333 32-33 22.700092662409173 27.165861928498735 27.341392212311742 22.79265319678035 34-35 22.73164810823319 26.989997220237257 27.39933547661288 22.879019194916673 36-37 22.65993807463789 27.058792710125996 27.553395161671766 22.72787405356435 38-39 22.72461396436263 26.95716869892342 27.58767986822168 22.73053746849227 40-41 22.543302462689986 26.96899861667888 27.512081319780034 22.975617600851095 42-43 22.438913850657087 27.130665444904867 27.358028407126117 23.072392297311932 44-45 22.655219176874024 26.859351136205863 27.54990302758278 22.935526659337338 46-47 22.571510954889813 26.74072541985363 27.52611926883659 23.161644356419963 48-49 22.55719611495945 26.838999393480943 27.486171380131413 23.11763311142819 50-51 22.460769582600996 26.996297569765204 27.37443049332838 23.168502354305424 52-53 22.503561136914342 26.80765638977508 27.467811471191734 23.220971002118848 54-55 22.519323753770358 26.871189433776387 27.43303461609114 23.176452196362117 56-57 22.459454377040963 26.94823341191046 27.40057101837238 23.1917411926762 58-59 22.56827166119692 26.92381975201515 27.431750170248893 23.076158416539037 60-61 22.511897869139595 26.84597088038555 27.338288971579892 23.303842278894958 62-63 22.44757333445121 26.76256242933215 27.447713069025387 23.342151167191254 64-65 22.474692523641586 26.806675144824688 27.310532163314534 23.408100168219192 66-67 22.394187528338566 26.815214725355187 27.466999900291917 23.323597846014334 68-69 22.421960811716744 26.59044175075646 27.594249357088835 23.39334808043796 70-71 22.33627603228631 26.542106938283695 27.568562109564844 23.553054919865154 72-73 22.374067316341133 26.28127490457615 27.57469625155544 23.769961527527276 74-75 22.30520320315422 26.28815921163165 27.49915819914037 23.90747938607376 76-77 22.18604821197902 26.165810768513957 27.7183559822642 23.92978503724282 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4370.0 1 2697.5 2 996.0 3 1016.0 4 1065.0 5 1121.5 6 1224.0 7 1343.0 8 1493.0 9 1570.0 10 1656.5 11 1884.0 12 2093.0 13 2161.0 14 2313.0 15 2705.0 16 3232.5 17 3520.0 18 3918.0 19 4798.5 20 5942.5 21 7964.0 22 9324.0 23 11311.0 24 15590.0 25 21331.0 26 24780.0 27 28219.0 28 37921.0 29 47737.0 30 51290.0 31 58434.0 32 71200.0 33 83197.0 34 96864.5 35 104157.0 36 110386.0 37 121976.0 38 129922.5 39 132508.0 40 136660.0 41 145227.5 42 153103.0 43 156563.0 44 158698.0 45 161572.5 46 163884.0 47 166857.5 48 168259.0 49 167870.0 50 165406.0 51 159189.5 52 155048.0 53 153449.0 54 144501.0 55 129315.5 56 114378.5 57 107278.0 58 100731.0 59 84481.0 60 67166.5 61 59555.0 62 52258.5 63 38251.0 64 26891.0 65 22242.0 66 18884.0 67 13533.5 68 9712.5 69 6672.5 70 5461.0 71 4557.5 72 2971.0 73 2026.0 74 1764.0 75 1493.0 76 977.5 77 608.0 78 483.0 79 414.5 80 282.5 81 206.0 82 157.0 83 121.0 84 113.5 85 92.5 86 69.0 87 59.0 88 62.5 89 58.5 90 51.5 91 52.0 92 48.0 93 48.0 94 48.0 95 44.5 96 45.0 97 45.0 98 49.5 99 100.5 100 147.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008964008052473209 2 0.012893037555992309 3 0.01446464935739995 4 0.015832533703069564 5 0.012863933633744018 6 0.009749813953177028 7 0.01647281999253193 8 0.01830636709417418 9 0.018044431793939573 10-11 0.02083840832977538 12-13 0.016676547448269963 14-15 0.00797447469603136 16-17 0.006461070739120299 18-19 0.017287729815484043 20-21 0.02201711718083111 22-23 0.01981977105108524 24-25 0.018044431793939573 26-27 0.023181274070762696 28-29 0.00830916980188669 30-31 0.008469241374252284 32-33 0.01087031495973618 34-35 0.012165439499785068 36-37 0.010928522804232758 38-39 0.014450097396275804 40-41 0.011365081637957103 42-43 0.014173610134917051 44-45 0.013096765011730337 46-47 0.02350141721549388 48-49 0.022890234848279796 50-51 0.03469187531996125 52-53 0.03828620971762502 54-55 0.027415894757888838 56-57 0.020285433807057874 58-59 0.016050813119931735 60-61 0.019907082817830112 62-63 0.02558234765624659 64-65 0.025073029016901522 66-67 0.02722671926327495 68-69 0.04046900388624674 70-71 0.0527945149583974 72-73 0.08320811370786006 74-75 0.0828588666408806 76-77 0.08787929322871056 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3435963.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.01331525377186 #Duplication Level Percentage of deduplicated Percentage of total 1 76.52236415405402 45.158383997871205 2 12.896042055095403 15.2207639064649 3 4.62030685880739 8.179788756838946 4 2.153356318450032 5.083067810975733 5 1.124654168533753 3.318478549957552 6 0.6414695236970649 2.2713145936573067 7 0.4452453747002717 1.8392783963729638 8 0.2967500383712131 1.4009762852775438 9 0.22060341770905706 1.171668513179169 >10 0.9930715445018689 10.233040575727085 >50 0.059419801976284305 2.401918969959687 >100 0.024783273374225957 2.5775643539222592 >500 0.0012889804888529537 0.5271443920697693 >1k 6.444902405439586E-4 0.6166108977259083 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4760 0.13853466990185867 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 2.9103922248289634E-5 0.0 5.820784449657927E-5 0.0 2 0.0 2.9103922248289634E-5 0.0 2.3283137798631707E-4 0.0 3 0.0 2.9103922248289634E-5 0.0 2.9103922248289635E-4 0.0 4 0.0 2.9103922248289634E-5 0.0 0.0012805725789247439 2.9103922248289634E-5 5 0.0 2.9103922248289634E-5 0.0 0.0028812883025806737 2.9103922248289634E-5 6 0.0 2.9103922248289634E-5 0.0 0.005238706004692134 2.9103922248289634E-5 7 0.0 2.9103922248289634E-5 0.0 0.011001282609853481 5.820784449657927E-5 8 0.0 2.9103922248289634E-5 0.0 0.0182481592496776 8.731176674486891E-5 9 0.0 2.9103922248289634E-5 0.0 0.02473833391104619 8.731176674486891E-5 10 0.0 2.9103922248289634E-5 0.0 0.03332399097429163 1.1641568899315854E-4 11 0.0 2.9103922248289634E-5 0.0 0.042782765704985765 1.1641568899315854E-4 12 0.0 2.9103922248289634E-5 0.0 0.056519817006178474 1.1641568899315854E-4 13 0.0 5.820784449657927E-5 0.0 0.07476797625585607 1.1641568899315854E-4 14 0.0 5.820784449657927E-5 0.0 0.09840036112146726 1.1641568899315854E-4 15 0.0 8.731176674486891E-5 0.0 0.12488493036741083 1.4551961124144818E-4 16 0.0 8.731176674486891E-5 0.0 0.15064190155714716 1.4551961124144818E-4 17 0.0 8.731176674486891E-5 0.0 0.17674811981386296 1.4551961124144818E-4 18 0.0 8.731176674486891E-5 0.0 0.20326179298205482 1.4551961124144818E-4 19 0.0 8.731176674486891E-5 0.0 0.23373359957601406 1.4551961124144818E-4 20 0.0 8.731176674486891E-5 0.0 0.2662717846496019 1.4551961124144818E-4 21 0.0 8.731176674486891E-5 0.0 0.30058530898033536 1.4551961124144818E-4 22 0.0 8.731176674486891E-5 0.0 0.3377801216136495 1.4551961124144818E-4 23 0.0 8.731176674486891E-5 0.0 0.3799226010291729 1.4551961124144818E-4 24 0.0 8.731176674486891E-5 0.0 0.42043526079879207 1.4551961124144818E-4 25 0.0 8.731176674486891E-5 0.0 0.4652553010611581 1.4551961124144818E-4 26 0.0 8.731176674486891E-5 0.0 0.5113850178246971 1.4551961124144818E-4 27 0.0 8.731176674486891E-5 0.0 0.5597557366013546 1.4551961124144818E-4 28 0.0 8.731176674486891E-5 0.0 0.6078936240000256 1.4551961124144818E-4 29 0.0 8.731176674486891E-5 0.0 0.6560315113986966 1.4551961124144818E-4 30 0.0 8.731176674486891E-5 0.0 0.7065850243439757 1.4551961124144818E-4 31 0.0 8.731176674486891E-5 0.0 0.7631048413501542 1.4551961124144818E-4 32 0.0 8.731176674486891E-5 0.0 0.8216328289914647 1.4551961124144818E-4 33 0.0 8.731176674486891E-5 0.0 0.8851957951817293 1.7462353348973782E-4 34 0.0 8.731176674486891E-5 0.0 0.9542885065991689 1.7462353348973782E-4 35 0.0 8.731176674486891E-5 0.0 1.0284161965655625 1.7462353348973782E-4 36 0.0 8.731176674486891E-5 0.0 1.1041446022556123 1.7462353348973782E-4 37 0.0 8.731176674486891E-5 0.0 1.1816483472028074 1.7462353348973782E-4 38 0.0 8.731176674486891E-5 0.0 1.2596468588282237 2.0372745573802746E-4 39 0.0 8.731176674486891E-5 0.0 1.3372961233866605 2.0372745573802746E-4 40 0.0 8.731176674486891E-5 0.0 1.4214064586842174 2.0372745573802746E-4 41 0.0 8.731176674486891E-5 0.0 1.5098241744745213 2.0372745573802746E-4 42 0.0 1.1641568899315854E-4 0.0 1.6053141433711597 2.0372745573802746E-4 43 0.0 1.1641568899315854E-4 0.0 1.710030055620506 2.0372745573802746E-4 44 0.0 1.1641568899315854E-4 0.0 1.8179182953949156 2.0372745573802746E-4 45 0.0 1.1641568899315854E-4 0.0 1.9304631627290516 2.0372745573802746E-4 46 0.0 1.1641568899315854E-4 0.0 2.0520302459601574 2.3283137798631707E-4 47 0.0 1.1641568899315854E-4 0.0 2.1755763959041468 2.3283137798631707E-4 48 0.0 1.1641568899315854E-4 0.0 2.3047104989198077 2.3283137798631707E-4 49 0.0 1.1641568899315854E-4 0.0 2.4317491195335923 2.3283137798631707E-4 50 0.0 1.1641568899315854E-4 0.0 2.561552612760964 2.3283137798631707E-4 51 0.0 1.1641568899315854E-4 0.0 2.700931296408023 2.3283137798631707E-4 52 0.0 1.4551961124144818E-4 0.0 2.8472949213946714 2.3283137798631707E-4 53 0.0 1.4551961124144818E-4 0.0 3.006522480015064 2.3283137798631707E-4 54 0.0 1.4551961124144818E-4 0.0 3.174917774143668 2.3283137798631707E-4 55 0.0 1.4551961124144818E-4 0.0 3.3492211644886747 2.3283137798631707E-4 56 0.0 1.4551961124144818E-4 0.0 3.5351952276552456 2.3283137798631707E-4 57 0.0 1.4551961124144818E-4 0.0 3.7213730182775544 2.9103922248289635E-4 58 0.0 1.4551961124144818E-4 0.0 3.9154670757513976 2.9103922248289635E-4 59 0.0 1.4551961124144818E-4 0.0 4.109182782236013 2.9103922248289635E-4 60 0.0 1.4551961124144818E-4 0.0 4.310407300660688 2.9103922248289635E-4 61 0.0 1.4551961124144818E-4 0.0 4.515269809366399 2.9103922248289635E-4 62 0.0 1.4551961124144818E-4 0.0 4.73119180852646 2.9103922248289635E-4 63 0.0 1.4551961124144818E-4 0.0 4.957794947151643 2.9103922248289635E-4 64 0.0 1.4551961124144818E-4 0.0 5.191208403582926 2.9103922248289635E-4 65 0.0 1.4551961124144818E-4 0.0 5.431839632731784 3.2014314473118597E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCG 75 0.0016871234 23.669992 52 CGATCGT 285 0.0 23.661722 1 ATTCGCG 310 0.0 21.757954 56 CTCGCGC 335 0.0 20.131588 36 TCGCGCT 375 0.0 18.932135 37 CGCGCTT 400 0.0 18.635504 38 CGCGGAA 345 0.0 18.522184 59 AACGCGA 100 0.008931133 17.750942 53 CGCTCCG 440 0.0 17.74784 25 TCGCGGA 385 0.0 17.519135 58 CAATTCG 450 0.0 17.35572 54 TATAGCG 190 5.3548247E-6 16.815948 21 CCTCGCG 425 0.0 16.703608 35 AATTCGC 450 0.0 16.564413 55 AACTCGA 455 0.0 16.382147 13 TCGAACT 1030 0.0 16.19871 24 CGCCGTA 155 3.6255943E-4 16.04222 71 CGATTCG 205 1.1041802E-5 15.582109 5 GCCTCGA 1095 0.0 15.56179 21 CGAACTC 1055 0.0 15.476791 25 >>END_MODULE