FastQCFastQC Report
Thu 26 May 2016
SRR936113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936113_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3435963
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62570.18210324150754825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44410.1292505187046543No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71500.037.534121
CGTATCA13200.034.723057100-101
CGGTATC8750.034.694714100-101
CGTACAT18400.031.380154100-101
GTATCAA135900.030.781631
TTATACG8150.030.67044896-97
CCTGTCG11200.030.55471896-97
GCGTACA6650.028.196169100-101
TCGAGTA6100.027.810972100-101
TACGCAG10700.027.25922100-101
CCGTACA8050.025.880539100-101
CGAGTAC13350.025.861153100-101
GGGTATC12750.024.743826100-101
TCGTACA8650.024.085358100-101
ATCAACG174000.023.7644673
TATCAAC175850.023.5828112
TCAACGC176150.023.4740684
ATACACG10800.022.86929596-97
CAACGCA180950.022.8503825
CCGAGCG9550.022.74667596-97
Produced by FastQC (version 0.11.5)