FastQCFastQC Report
Thu 26 May 2016
SRR936112_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936112_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3444514
Sequences flagged as poor quality0
Sequence length77
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47730.13856816955889859No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA2401.8189894E-1120.7104359
TCGCGGT2950.020.45345933
TAACGCG950.00664212618.68637552
ATTCGCG2751.3096724E-1018.07245856
CTTCGCG4150.017.104731
CGATCGT3155.0931703E-1116.9020541
CGCGGTT3800.016.81236534
GACGCGA5800.015.92234169
TCGCGCT3602.1827873E-1115.77616937
CTCGCGC3602.1827873E-1115.77570936
CGGTTAC4059.094947E-1214.8990644
TCGCGGA3402.6684575E-914.61912758
CCGGTTA2504.5610723E-614.19690231
TAAGGCG11400.014.01111817
TTAGTCG2303.2713826E-513.88968515
GGACGCG6650.013.8855468
CGTGCGA1550.006711467613.74372650
CCTCGCG3901.2587407E-913.65106635
CGCGACC6550.013.55867171
ACGCGAC6850.013.48247470
Produced by FastQC (version 0.11.5)