##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936112_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3444514 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.27216205246952 39.0 36.0 41.0 29.0 41.0 2 36.13408335689737 39.0 36.0 41.0 28.0 41.0 3 35.99155700920362 39.0 35.0 41.0 27.0 41.0 4 35.88694747647999 39.0 35.0 41.0 27.0 41.0 5 35.8105459870391 39.0 35.0 40.0 27.0 41.0 6 35.72653878021689 39.0 35.0 40.0 27.0 41.0 7 35.58287206845436 39.0 35.0 40.0 27.0 41.0 8 35.46254159512779 38.0 35.0 40.0 27.0 41.0 9 35.63327976022162 39.0 35.0 40.0 27.0 41.0 10-11 35.861124965670044 39.0 35.0 41.0 28.0 41.0 12-13 35.77675631453378 39.0 35.0 41.0 27.0 41.0 14-15 35.625225503510805 39.0 35.0 41.0 26.5 41.0 16-17 35.477607000581216 39.0 35.0 41.0 26.0 41.0 18-19 35.33061964619682 39.0 35.0 41.0 26.0 41.0 20-21 35.14670182789212 39.0 35.0 41.0 25.0 41.0 22-23 34.897327170102955 39.0 34.0 41.0 24.0 41.0 24-25 34.68448843581417 39.0 34.0 40.5 24.0 41.0 26-27 33.73895649720106 38.0 33.0 39.5 22.0 40.5 28-29 33.75253504558263 38.0 33.0 39.5 22.0 40.5 30-31 34.158911823264475 38.0 33.0 40.0 22.5 41.0 32-33 34.03375904409156 38.0 33.0 40.0 21.5 41.0 34-35 33.770314622033766 38.0 33.0 40.0 20.5 41.0 36-37 33.45937249783279 37.5 33.0 40.0 19.0 41.0 38-39 33.14557322165042 37.0 32.0 40.0 18.0 41.0 40-41 32.78337568086528 36.5 32.0 40.0 15.5 41.0 42-43 32.40829228738801 36.0 32.0 39.0 13.0 41.0 44-45 32.0431297419607 35.5 31.0 39.0 9.0 40.5 46-47 31.616811544386234 35.0 31.0 38.5 7.5 40.0 48-49 31.192103878805547 35.0 31.0 37.5 4.5 40.0 50-51 30.733214032516635 35.0 30.0 37.0 2.0 39.0 52-53 30.24035103355655 35.0 30.0 36.5 2.0 39.0 54-55 29.767683771934152 34.0 29.5 36.0 2.0 38.5 56-57 29.32305646021471 34.0 29.0 35.5 2.0 37.0 58-59 28.881370927799974 34.0 29.0 35.0 2.0 37.0 60-61 28.50957377441346 34.0 29.0 35.0 2.0 36.5 62-63 28.12589003847858 34.0 27.5 35.0 2.0 36.0 64-65 27.730213899551575 34.0 27.0 35.0 2.0 36.0 66-67 27.35420947628606 33.5 26.0 35.0 2.0 35.0 68-69 26.97437621098361 33.0 25.5 35.0 2.0 35.0 70-71 26.501962541014493 33.0 25.0 35.0 2.0 35.0 72-73 25.995640313843985 33.0 24.0 35.0 2.0 35.0 74-75 25.396526186277658 33.0 21.0 35.0 2.0 35.0 76-77 23.613285938161376 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.2606911265910625 1101 2 1.2013505490667171 1101 3 1.2578220590433205 1101 4 0.981884763599524 1101 5 1.1238597601670008 1101 6 1.212567651658432 1101 7 1.1499405398093714 1101 8 1.1959103509899762 1101 9 1.1315404003105058 1101 10-11 1.2320652253255417 1101 12-13 1.3739227850238578 1101 14-15 1.3723230629574772 1101 16-17 1.3941346176625302 1101 18-19 1.3228822851021107 1101 20-21 1.2247074626812307 1101 22-23 1.1425768392674982 1101 24-25 1.2455525369470806 1101 26-27 1.310444034714692 1101 28-29 1.3790122175034867 1101 30-31 1.3057635005346384 1101 32-33 1.4789788812689366 1101 34-35 1.5180252354682153 1101 36-37 1.5289176608311053 1101 38-39 1.5284857604734867 1101 40-41 1.4929786424272606 1101 42-43 1.526578428925042 1101 44-45 1.5031006650438599 1101 46-47 1.569643168523644 1101 48-49 1.6058007972574266 1101 50-51 1.4233633727520818 1101 52-53 1.4856703990332996 1101 54-55 1.568262164902933 1101 56-57 1.4870730373543068 1101 58-59 1.6634729439177605 1101 60-61 1.5946160717907993 1101 62-63 1.6909476736579698 1101 64-65 1.595880868133662 1101 66-67 1.739538510262264 1101 68-69 1.6260862702725802 1101 70-71 1.518094475555408 1101 72-73 1.5234616672338746 1101 74-75 1.641411174715131 1101 76-77 1.6237748898035669 1102 1 0.8225970059580305 1102 2 0.9276249146760875 1102 3 1.0282399597378102 1102 4 0.829419984235372 1102 5 0.8515336104658786 1102 6 0.9805420289245887 1102 7 0.8504242104462989 1102 8 0.8444877154185448 1102 9 0.8244300265907683 1102 10-11 0.9782463825557244 1102 12-13 1.073298247156231 1102 14-15 1.0228638499496512 1102 16-17 1.0320375975705574 1102 18-19 1.0525268314950722 1102 20-21 1.0303730835545721 1102 22-23 0.8726129121505792 1102 24-25 1.0344748734424272 1102 26-27 0.8344797990469459 1102 28-29 1.028795169611307 1102 30-31 1.178442885415997 1102 32-33 1.079232009700533 1102 34-35 1.1170526304551913 1102 36-37 1.0876092617983275 1102 38-39 1.223237314855659 1102 40-41 1.2138247052364903 1102 42-43 1.2403696935297646 1102 44-45 1.0686494347357467 1102 46-47 1.128269020603387 1102 48-49 1.1428874286423643 1102 50-51 1.252274862116554 1102 52-53 1.2213604934226687 1102 54-55 1.321609280938521 1102 56-57 1.5280495719878928 1102 58-59 1.5117123571505715 1102 60-61 1.3888248293894883 1102 62-63 1.441129033398557 1102 64-65 1.189031585447328 1102 66-67 1.2570704941770394 1102 68-69 1.2010988601796377 1102 70-71 1.1209694724689427 1102 72-73 1.0912939426832438 1102 74-75 1.126745918858166 1102 76-77 1.1718197648069903 1103 1 0.6589975043321417 1103 2 0.7777142241221355 1103 3 0.7508062455560989 1103 4 0.7184746881659194 1103 5 0.585915238000247 1103 6 0.6247107119701596 1103 7 0.7567352073009062 1103 8 0.6943272352273908 1103 9 0.7229873760976844 1103 10-11 0.7888765086924323 1103 12-13 0.8070363815600032 1103 14-15 0.8507143023155734 1103 16-17 0.8423615831096853 1103 18-19 0.7947010859888195 1103 20-21 0.7783797801388701 1103 22-23 0.7776419128875105 1103 24-25 0.9142021517418826 1103 26-27 0.941473654673807 1103 28-29 0.7662269046832719 1103 30-31 0.9836145006887307 1103 32-33 0.9818479935821856 1103 34-35 1.0928649810980744 1103 36-37 1.0406539884547428 1103 38-39 0.9841145331274603 1103 40-41 0.979159392750411 1103 42-43 0.8626620110531675 1103 44-45 0.91481545568098 1103 46-47 0.8875727430527398 1103 48-49 0.820132336506898 1103 50-51 0.8886614163348838 1103 52-53 0.947452033180415 1103 54-55 0.9542904652822379 1103 56-57 0.8664381247116317 1103 58-59 0.8678275002871878 1103 60-61 0.7280317656513198 1103 62-63 0.7423393220953081 1103 64-65 0.642322429748539 1103 66-67 0.7440409469723086 1103 68-69 0.6993264647685713 1103 70-71 0.6550805400706068 1103 72-73 0.6206725182269999 1103 74-75 0.6644320947577143 1103 76-77 0.7167295971504046 1104 1 0.37466401620118006 1104 2 0.3597969230646427 1104 3 0.44071484838585917 1104 4 0.3765782887268543 1104 5 0.2758244821300835 1104 6 0.36795111145613646 1104 7 0.4243041220938437 1104 8 0.30248711903114867 1104 9 0.20191851576945652 1104 10-11 0.3299145829525969 1104 12-13 0.48956746835199993 1104 14-15 0.5833210304511596 1104 16-17 0.4731933025808459 1104 18-19 0.24032873415730904 1104 20-21 0.39918852915757697 1104 22-23 0.3000363404662707 1104 24-25 0.3149527659928353 1104 26-27 0.14049219988093142 1104 28-29 0.29730641334113983 1104 30-31 0.44160930011160104 1104 32-33 0.4368401344497883 1104 34-35 0.43355944677922054 1104 36-37 0.4380092852772677 1104 38-39 0.4401567570571743 1104 40-41 0.518113470809638 1104 42-43 0.5858351235032089 1104 44-45 0.4797324297670542 1104 46-47 0.34929367912729603 1104 48-49 0.22750845084593507 1104 50-51 0.1840104242024445 1104 52-53 0.1251049793541732 1104 54-55 0.06145812045189203 1104 56-57 0.1251078832997159 1104 58-59 0.07046943671291217 1104 60-61 0.1751365111161185 1104 62-63 0.23206271746403928 1104 64-65 0.2919095653423689 1104 66-67 0.21217277664630885 1104 68-69 0.09003633539360933 1104 70-71 0.07468235319081984 1104 72-73 0.1730634818692245 1104 74-75 0.06400784727525277 1104 76-77 0.1540643650414637 1105 1 0.1860269062436899 1105 2 0.5548294830570342 1105 3 0.37202528714274763 1105 4 0.3969737279951602 1105 5 0.2697234088795213 1105 6 0.30196226206471266 1105 7 0.4092880349992285 1105 8 0.4200332673697744 1105 9 0.2735922196054119 1105 10-11 0.3222286213427097 1105 12-13 0.5193791066875519 1105 14-15 0.44971129457379533 1105 16-17 0.39983933625517665 1105 18-19 0.34860510918669263 1105 20-21 0.354648047639337 1105 22-23 0.4564020477690818 1105 24-25 0.5550297292374538 1105 26-27 0.331870665604562 1105 28-29 0.4198249363789728 1105 30-31 0.571414702791408 1105 32-33 0.5170562380531933 1105 34-35 0.5599735948092004 1105 36-37 0.6042143638824271 1105 38-39 0.4541022403788091 1105 40-41 0.5032783971750661 1105 42-43 0.661386283694064 1105 44-45 0.4910326940870817 1105 46-47 0.44956979755272286 1105 48-49 0.2155599923538709 1105 50-51 0.3570927133251267 1105 52-53 0.32427622033041814 1105 54-55 0.08117158534111368 1105 56-57 0.26702553137186413 1105 58-59 0.24247327141689468 1105 60-61 0.38869094255889536 1105 62-63 0.16730884193669837 1105 64-65 0.1889808707387708 1105 66-67 0.2809024883347071 1105 68-69 0.1596608861123734 1105 70-71 0.13022831849724525 1105 72-73 -0.18583865691583412 1105 74-75 -0.11191740739969802 1105 76-77 -0.06360681043796035 1106 1 0.2976487330126929 1106 2 0.31754340193788266 1106 3 0.43112539678262607 1106 4 0.4185321394910133 1106 5 0.4454379073623258 1106 6 0.31820583126434343 1106 7 0.05600924645703742 1106 8 0.1760262260134695 1106 9 0.2999107243400232 1106 10-11 0.037735979416488874 1106 12-13 0.4004155354781602 1106 14-15 0.45386343380746297 1106 16-17 0.34718191510706475 1106 18-19 0.3714345177953078 1106 20-21 0.34884390824057476 1106 22-23 0.4513698735798499 1106 24-25 0.3230438657830632 1106 26-27 0.28538282019857775 1106 28-29 0.4675191792985842 1106 30-31 0.5683698634918812 1106 32-33 0.4548203562058575 1106 34-35 0.4960407173815611 1106 36-37 0.38511746627816734 1106 38-39 0.6686554085405376 1106 40-41 0.5717544657152445 1106 42-43 0.6193982041145389 1106 44-45 0.5275298526019441 1106 46-47 0.5442292499993435 1106 48-49 0.5079999102406028 1106 50-51 0.4315381437109842 1106 52-53 0.4324169362016441 1106 54-55 0.39903678392207453 1106 56-57 0.3600494475790086 1106 58-59 0.19363395574197995 1106 60-61 0.079092159902455 1106 62-63 0.08267704170737389 1106 64-65 0.1350321305206741 1106 66-67 -0.08734775077352808 1106 68-69 -0.005858180638750099 1106 70-71 -0.08953700551460386 1106 72-73 -0.02726615552963807 1106 74-75 -0.05840749584731242 1106 76-77 -0.15384693253120219 1107 1 -0.017503351870374217 1107 2 0.0325144497458254 1107 3 0.2263482720493144 1107 4 -0.001235251608022736 1107 5 0.1148497551423091 1107 6 -0.07100231371697419 1107 7 -0.05177990457143267 1107 8 -0.0013578447762156998 1107 9 -0.08444938725631346 1107 10-11 0.031341243457731593 1107 12-13 -0.05476170049668383 1107 14-15 -0.10558982692088392 1107 16-17 -0.07572766670929099 1107 18-19 0.0018006913247887724 1107 20-21 -0.0523889231086585 1107 22-23 -0.019561318591641452 1107 24-25 -9.768878244145185E-4 1107 26-27 -0.2850984142276545 1107 28-29 -0.0568633006119299 1107 30-31 0.19571598415207347 1107 32-33 0.3002058413422546 1107 34-35 0.19568313915637248 1107 36-37 0.18961703503460825 1107 38-39 0.14580510858320395 1107 40-41 0.14300639909346557 1107 42-43 0.25000182683876204 1107 44-45 0.23558213264467298 1107 46-47 0.15259447947153149 1107 48-49 0.05552588181696194 1107 50-51 -0.02726466014615525 1107 52-53 0.15932649123376663 1107 54-55 0.032863037028565856 1107 56-57 0.09643105578444278 1107 58-59 0.1538236698654991 1107 60-61 0.036872466915870206 1107 62-63 0.03054444270266643 1107 64-65 -0.1116714520098121 1107 66-67 -0.153784000556346 1107 68-69 -0.20349104565960374 1107 70-71 -0.3598248890748401 1107 72-73 -0.3027454411419299 1107 74-75 -0.5717387397784641 1107 76-77 -0.4907768174929217 1108 1 0.14038914582613415 1108 2 0.369882377837925 1108 3 0.36479644761701024 1108 4 0.35817266497573286 1108 5 0.42634627267845104 1108 6 0.4704358112583975 1108 7 0.4386623155311469 1108 8 0.43712287594497923 1108 9 0.39386934931632567 1108 10-11 0.44477312161768623 1108 12-13 0.47378284911712853 1108 14-15 0.4502299947661115 1108 16-17 0.42024789164866405 1108 18-19 0.32714967436324116 1108 20-21 0.31506415211037364 1108 22-23 0.3509193167109501 1108 24-25 0.48906787086634296 1108 26-27 0.3663895912444346 1108 28-29 0.4495507101594214 1108 30-31 0.522371573989119 1108 32-33 0.4568327033769677 1108 34-35 0.5221913156709519 1108 36-37 0.5167427583200208 1108 38-39 0.6313211909545586 1108 40-41 0.5906742557716171 1108 42-43 0.6397529673262241 1108 44-45 0.4991322394344877 1108 46-47 0.5325849708569983 1108 48-49 0.5975001906794084 1108 50-51 0.5029901654260591 1108 52-53 0.656498655095799 1108 54-55 0.7025935446170841 1108 56-57 0.6843715865518654 1108 58-59 0.7316487994253364 1108 60-61 0.7057919943059439 1108 62-63 0.6509187185895549 1108 64-65 0.7418876071803169 1108 66-67 0.7399986997908563 1108 68-69 0.7962327509394775 1108 70-71 0.742154836254656 1108 72-73 0.8194410005405004 1108 74-75 0.8620427157083377 1108 76-77 0.8845101046195438 1109 1 -0.12823896478401764 1109 2 -0.2407292552079241 1109 3 -0.2102357533843886 1109 4 -0.09047509046716584 1109 5 -0.07508034771576888 1109 6 -0.2608193875615967 1109 7 -0.16127303558896955 1109 8 -0.09567277006487984 1109 9 -0.047424431454842875 1109 10-11 -0.19327001208864658 1109 12-13 -0.12856654845340643 1109 14-15 0.026330186046472193 1109 16-17 -0.059004228778633205 1109 18-19 -0.009771386523560466 1109 20-21 -0.030674123943633447 1109 22-23 -0.029524281914312667 1109 24-25 -0.14931340215699862 1109 26-27 -0.1382800888588136 1109 28-29 0.070131075399253 1109 30-31 0.09990472217560864 1109 32-33 0.105662061605166 1109 34-35 0.22527899094134796 1109 36-37 0.08540495442667151 1109 38-39 0.056674447378547654 1109 40-41 0.0589059476137237 1109 42-43 0.069146614158349 1109 44-45 0.052566809929260216 1109 46-47 -0.017366410815309763 1109 48-49 0.13243124120066696 1109 50-51 0.043813760555536874 1109 52-53 0.06003678229938103 1109 54-55 0.1942474532245022 1109 56-57 0.1354115809691514 1109 58-59 0.24175932135970868 1109 60-61 0.11330369051940892 1109 62-63 -0.03191639483729958 1109 64-65 0.12265996379328215 1109 66-67 0.11853780627355448 1109 68-69 0.1589855489620895 1109 70-71 0.07944621277216868 1109 72-73 0.08029799649401781 1109 74-75 0.12087885811889265 1109 76-77 0.11729742264232712 1110 1 -0.28140972733845615 1110 2 -0.3021264680544391 1110 3 -0.49229347259804257 1110 4 -0.31074590123952106 1110 5 -0.417708303039106 1110 6 -0.4742464164686311 1110 7 -0.45022718114383053 1110 8 -0.5983261781228038 1110 9 -0.5381615331630201 1110 10-11 -0.49296742492093415 1110 12-13 -0.33329253632778233 1110 14-15 -0.37159130570509546 1110 16-17 -0.4423503938736033 1110 18-19 -0.43165409842548286 1110 20-21 -0.527433405891955 1110 22-23 -0.6020332733694644 1110 24-25 -0.486530438099102 1110 26-27 -0.443811394931906 1110 28-29 -0.25914889677662956 1110 30-31 -0.1607044834935607 1110 32-33 -0.36690241997322204 1110 34-35 -0.44713656842597516 1110 36-37 -0.2948570822663754 1110 38-39 -0.42423710832105144 1110 40-41 -0.28242376015410997 1110 42-43 -0.32076817451281414 1110 44-45 -0.27652123914235105 1110 46-47 -0.4227921907993384 1110 48-49 -0.34287122739840825 1110 50-51 -0.3845113388445043 1110 52-53 -0.39456636846459503 1110 54-55 -0.37873818416098004 1110 56-57 -0.3558466694320046 1110 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-0.7669847730284758 2314 48-49 -0.7525586785838776 2314 50-51 -0.7007104953294494 2314 52-53 -0.6167421890580442 2314 54-55 -0.618342574242547 2314 56-57 -0.6688562251943253 2314 58-59 -0.6656909405656855 2314 60-61 -0.6341557289963582 2314 62-63 -0.7198235120959389 2314 64-65 -0.5498060571407244 2314 66-67 -0.4993712477825447 2314 68-69 -0.5232456359946305 2314 70-71 -0.44544685569576714 2314 72-73 -0.39965185847503903 2314 74-75 -0.45544962902486574 2314 76-77 -0.35991209278353153 2315 1 -1.3365139708213292 2315 2 -1.1068637269011177 2315 3 -1.1323981408709827 2315 4 -1.0313591621659697 2315 5 -1.2127262291800065 2315 6 -1.13692884681155 2315 7 -1.5793086490823782 2315 8 -1.5395479423269336 2315 9 -1.1530158641526498 2315 10-11 -1.302496859474374 2315 12-13 -1.1695588695059271 2315 14-15 -1.1904343134906483 2315 16-17 -1.0868463719612365 2315 18-19 -1.1595880554639137 2315 20-21 -1.113054465964069 2315 22-23 -1.1622182779402053 2315 24-25 -1.3531363994330192 2315 26-27 -1.1038120745960143 2315 28-29 -1.0863836833451117 2315 30-31 -1.21420553527377 2315 32-33 -1.0616945956799881 2315 34-35 -1.2412575078577106 2315 36-37 -1.2408773430534907 2315 38-39 -1.3110569599503776 2315 40-41 -1.3086546828321204 2315 42-43 -1.3873256078292684 2315 44-45 -1.3191958193648041 2315 46-47 -1.2351740736728267 2315 48-49 -1.1512270859331188 2315 50-51 -1.1507846725629811 2315 52-53 -1.1551836394092945 2315 54-55 -1.3294272616188998 2315 56-57 -1.379445864709698 2315 58-59 -1.2462419921764862 2315 60-61 -1.3286979462474626 2315 62-63 -1.377123862983403 2315 64-65 -1.1159790432214542 2315 66-67 -1.1512422150642507 2315 68-69 -1.5147826851920563 2315 70-71 -1.3652146333564268 2315 72-73 -1.341349948894024 2315 74-75 -1.3310308797851143 2315 76-77 -1.2136754773710265 2316 1 -2.6066400245118686 2316 2 -2.25187133435837 2316 3 -2.51019366775558 2316 4 -3.318640418403234 2316 5 -2.7166305370852584 2316 6 -2.6768145241925794 2316 7 -2.3200495840256963 2316 8 -2.1088162529143872 2316 9 -2.494815653092779 2316 10-11 -2.3593397133028517 2316 12-13 -2.948744651286482 2316 14-15 -4.228280621676053 2316 16-17 -3.23768488787411 2316 18-19 -2.7814677398996537 2316 20-21 -2.5018686335193436 2316 22-23 -2.7818551874174204 2316 24-25 -2.759321228468327 2316 26-27 -2.6372141677482226 2316 28-29 -3.45479064168806 2316 30-31 -3.521579120158357 2316 32-33 -3.512096957066312 2316 34-35 -3.589286572750275 2316 36-37 -3.5236305227922067 2316 38-39 -3.640697687626833 2316 40-41 -3.2583105988652434 2316 42-43 -3.3309177576878994 2316 44-45 -3.327494018614331 2316 46-47 -3.3348765033672585 2316 48-49 -3.129230730893571 2316 50-51 -3.231945036045744 2316 52-53 -3.2175635971523775 2316 54-55 -3.2009675675910145 2316 56-57 -3.034786614306583 2316 58-59 -3.3591856807857567 2316 60-61 -3.0662239873945403 2316 62-63 -3.1314701715433415 2316 64-65 -3.1329456851530146 2316 66-67 -2.9739819919498522 2316 68-69 -3.002554403319742 2316 70-71 -3.0148319315763104 2316 72-73 -3.002418439979408 2316 74-75 -3.1069270313031367 2316 76-77 -3.4061102334574187 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 100638.0 3 8147.0 4 10004.0 5 12484.0 6 14070.0 7 14741.0 8 16616.0 9 16646.0 10 19037.0 11 20513.0 12 17959.0 13 16704.0 14 16823.0 15 17667.0 16 18090.0 17 19077.0 18 20214.0 19 21411.0 20 23005.0 21 25016.0 22 27513.0 23 31163.0 24 36207.0 25 41802.0 26 49188.0 27 57890.0 28 67301.0 29 79038.0 30 92835.0 31 111745.0 32 135685.0 33 168890.0 34 220128.0 35 294532.0 36 417018.0 37 560143.0 38 495678.0 39 127957.0 40 939.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.69816089778243 26.55988071064958 28.284797517592335 22.457160873975656 2 22.471047896724457 26.730034611580027 28.198261262409545 22.600656229285967 3 22.68419234108284 26.979869864027943 27.65900426527762 22.676933529611595 4 22.425441577911652 27.017114310960167 27.911522099003932 22.645922012124245 5 22.65574845290301 26.97318926571799 27.843013027466938 22.52804925391206 6 22.83435737084889 26.932356033468764 27.801856362587387 22.43143023309496 7 22.649792038579427 26.642117036808184 27.941019330365386 22.767071594247 8 22.46445048998812 27.003660718978335 27.69724312174221 22.83464566929134 9 22.372823971046678 26.798229210706037 28.038077893357737 22.790868924889544 10-11 22.80402587452398 26.959757951560988 27.7536980872871 22.48251808662794 12-13 22.880793634707697 26.66059972687156 27.748859472425963 22.709747165994784 14-15 22.601967438533695 26.902601732262227 27.804952900137607 22.69047792906647 16-17 22.9315236210968 26.735383006932167 27.80954761694269 22.523545755028337 18-19 22.68014027988907 26.826082582329942 27.683037250939247 22.81073988684174 20-21 22.772135933098458 26.868322287409274 27.69596812641655 22.66357365307572 22-23 22.82497239054541 26.97378745968192 27.355913635216062 22.84532651455661 24-25 22.752357915277667 27.065963551043637 27.338619283797755 22.84305924988094 26-27 22.838571560684308 27.107632364403912 27.29494229788435 22.758853777027426 28-29 23.00528710335837 27.009520270132946 27.263829651329907 22.721362975178778 30-31 22.79535778917787 26.919062394660504 27.37338864143735 22.91219117472427 32-33 22.731059748773255 27.140644092446404 27.31605597211179 22.812240186668554 34-35 22.746575690487425 26.960897179980403 27.401981635393607 22.89054549413857 36-37 22.674612055474487 27.082456249357612 27.527598747799907 22.715332947367994 38-39 22.66639372346789 26.98990110164521 27.628080047852173 22.715625127034734 40-41 22.54281242409949 26.947956404416935 27.543328529416332 22.965902642067242 42-43 22.428275363949492 27.125766281091323 27.415880574479434 23.03007778047975 44-45 22.644344376491414 26.851791089241733 27.58568673374988 22.918177800516975 46-47 22.516789066374596 26.76427477156765 27.5399052471785 23.17903091487926 48-49 22.57794717875652 26.809587370148524 27.50643069124523 23.106034759849724 50-51 22.470080301313235 27.022709568568292 27.362761418489555 23.144448711628918 52-53 22.516119233246656 26.84067965343576 27.43580331311883 23.207397800198756 54-55 22.46786113571356 26.838209457987887 27.49685653303907 23.19707287325948 56-57 22.468422013119437 26.94184493579872 27.42644506410981 23.163287986972026 58-59 22.55732681091426 26.935568850173926 27.446629440638297 23.06047489827352 60-61 22.499340118665394 26.866041330494994 27.33915229131997 23.29546625951964 62-63 22.434296641380648 26.77588368576222 27.448348059916906 23.34147161294023 64-65 22.498861358387465 26.76949697811703 27.338418515728208 23.393223147767298 66-67 22.379286248680064 26.798392540141126 27.468317508602734 23.35400370257608 68-69 22.441364427522792 26.581004553135134 27.60409141815995 23.373539601182127 70-71 22.319446136926484 26.53975157381464 27.58339369381918 23.557408595439696 72-73 22.395005052121977 26.32649432703934 27.564249685803915 23.714250935034766 74-75 22.314962901710487 26.279605158502157 27.53408854341073 23.871343396376624 76-77 22.178863007495664 26.171247204035225 27.722474682872182 23.92741510559693 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4285.0 1 2660.5 2 1005.0 3 1001.5 4 1029.0 5 1108.0 6 1237.0 7 1353.5 8 1491.5 9 1563.0 10 1643.5 11 1844.5 12 2085.0 13 2205.0 14 2354.5 15 2678.5 16 3139.5 17 3426.0 18 3801.5 19 4791.5 20 6117.5 21 8031.5 22 9234.0 23 11216.5 24 15616.0 25 21335.5 26 24638.0 27 28320.0 28 38376.5 29 48273.0 30 51795.0 31 58933.0 32 71618.0 33 83664.0 34 97621.0 35 105079.0 36 111134.5 37 122221.0 38 130155.0 39 133058.0 40 137315.5 41 145547.0 42 153316.5 43 157112.0 44 158623.5 45 161632.0 46 164072.0 47 167145.0 48 169275.0 49 168640.5 50 166054.5 51 159874.5 52 155646.0 53 153386.5 54 144402.5 55 129820.5 56 114716.0 57 107469.0 58 100795.0 59 84276.5 60 67104.0 61 59776.0 62 52482.0 63 38387.5 64 26976.0 65 22365.0 66 18866.0 67 13427.5 68 9721.0 69 6748.0 70 5542.0 71 4541.0 72 2913.0 73 1973.5 74 1661.0 75 1389.5 76 941.0 77 647.5 78 531.0 79 453.0 80 302.0 81 191.5 82 135.0 83 116.0 84 108.0 85 88.5 86 65.5 87 54.0 88 52.0 89 46.5 90 40.5 91 38.0 92 36.0 93 31.5 94 31.5 95 34.0 96 34.0 97 34.0 98 28.0 99 70.5 100 119.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00336767393019741 2 0.00844821649730557 3 0.012309428848307772 4 0.01948025178588329 5 0.014022297485218523 6 0.013557790736225778 7 0.017593193118100262 8 0.019015745036890547 9 0.018173826554341195 10-11 0.02149795297682053 12-13 0.02069958200198925 14-15 0.008404668989587501 16-17 0.00608213524462377 18-19 0.01159815288891263 20-21 0.011844922099315027 22-23 0.01435616171105706 24-25 0.023051147418765027 26-27 0.014211003351996827 28-29 0.008244994794621244 30-31 0.008506279840929663 32-33 0.009711094221129599 34-35 0.01129332033488614 36-37 0.00975464172884767 38-39 0.01666417962011477 40-41 0.01304973647951496 42-43 0.015285175209042552 44-45 0.018348016585213473 46-47 0.017593193118100262 48-49 0.014022297485218523 50-51 0.026752685574800976 52-53 0.03228321905499586 54-55 0.02508336444560829 56-57 0.015691618614411207 58-59 0.017346423907697864 60-61 0.02069958200198925 62-63 0.026462368856680504 64-65 0.02083022452514346 66-67 0.020191527745278433 68-69 0.03084615130029955 70-71 0.05813592280362338 72-73 0.06955988566166374 74-75 0.08066450012977158 76-77 0.07906775818010901 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3444514.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.7037147653166 #Duplication Level Percentage of deduplicated Percentage of total 1 76.17733477580643 44.71892532260973 2 13.168423512326324 15.460707555529861 3 4.599145106193014 8.099607074347691 4 2.1995083291767537 5.164772383197209 5 1.1094558725901114 3.2564590544617666 6 0.6836310556647639 2.407900949787394 7 0.4275242118755237 1.7568081572445264 8 0.3006715897231861 1.4120431392915371 9 0.22626089444615816 1.1954119509101406 >10 1.0203260373095393 10.37024039793422 >50 0.06034933864013047 2.4142727355731592 >100 0.02548645189423098 2.620024271042871 >500 0.0012882482452281996 0.5344357565582538 >1k 5.945761085023351E-4 0.5883912515116106 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4773 0.13856816955889859 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.9031671812046633E-5 0.0 2 0.0 0.0 0.0 1.7419003087227981E-4 0.0 3 0.0 0.0 0.0 2.9031671812046635E-4 0.0 4 0.0 0.0 0.0 9.870768416095856E-4 0.0 5 0.0 0.0 0.0 0.0023515654167757774 0.0 6 0.0 0.0 0.0 0.00595149272146956 2.9031671812046633E-5 7 0.0 0.0 0.0 0.01216427048924754 2.9031671812046633E-5 8 0.0 0.0 0.0 0.019131871724138733 5.806334362409327E-5 9 0.0 0.0 0.0 0.026593011379834715 5.806334362409327E-5 10 0.0 0.0 0.0 0.03541863961069689 5.806334362409327E-5 11 0.0 0.0 0.0 0.045521661401289125 5.806334362409327E-5 12 0.0 0.0 0.0 0.05919557882476309 8.709501543613991E-5 13 0.0 0.0 0.0 0.07815326051802954 8.709501543613991E-5 14 0.0 0.0 0.0 0.10114634459317047 1.1612668724818653E-4 15 0.0 2.9031671812046633E-5 0.0 0.12660712077233538 1.4515835906023317E-4 16 0.0 2.9031671812046633E-5 0.0 0.15383882893203513 1.4515835906023317E-4 17 0.0 2.9031671812046633E-5 0.0 0.18078022037361438 1.4515835906023317E-4 18 0.0 2.9031671812046633E-5 0.0 0.2076054851279455 1.4515835906023317E-4 19 0.0 2.9031671812046633E-5 0.0 0.23727585371985713 1.7419003087227981E-4 20 0.0 2.9031671812046633E-5 0.0 0.26773007745069405 1.7419003087227981E-4 21 0.0 2.9031671812046633E-5 0.0 0.30216164021978137 1.7419003087227981E-4 22 0.0 2.9031671812046633E-5 0.0 0.3384802616566517 1.7419003087227981E-4 23 0.0 2.9031671812046633E-5 0.0 0.37921169720895315 1.7419003087227981E-4 24 0.0 2.9031671812046633E-5 0.0 0.41944959434044976 1.7419003087227981E-4 25 0.0 5.806334362409327E-5 0.0 0.46459384400818227 1.7419003087227981E-4 26 0.0 5.806334362409327E-5 0.0 0.5102896954403436 1.7419003087227981E-4 27 0.0 5.806334362409327E-5 0.0 0.5580177638993483 1.7419003087227981E-4 28 0.0 5.806334362409327E-5 0.0 0.6053684206247965 2.0322170268432643E-4 29 0.0 5.806334362409327E-5 0.0 0.6515578104777626 2.3225337449637307E-4 30 0.0 5.806334362409327E-5 0.0 0.7016955076971672 2.3225337449637307E-4 31 0.0 5.806334362409327E-5 0.0 0.7565653674219353 2.3225337449637307E-4 32 0.0 5.806334362409327E-5 0.0 0.8206092354393102 2.3225337449637307E-4 33 0.0 5.806334362409327E-5 0.0 0.8887756008539957 2.612850463084197E-4 34 0.0 5.806334362409327E-5 0.0 0.9558677944116354 2.9031671812046635E-4 35 0.0 8.709501543613991E-5 0.0 1.0254857434169233 2.9031671812046635E-4 36 0.0 8.709501543613991E-5 0.0 1.1010551851436807 2.9031671812046635E-4 37 0.0 8.709501543613991E-5 0.0 1.1797600474261392 2.9031671812046635E-4 38 0.0 8.709501543613991E-5 0.0 1.2577971812569204 2.9031671812046635E-4 39 0.0 8.709501543613991E-5 0.0 1.3374310570373644 2.9031671812046635E-4 40 0.0 8.709501543613991E-5 0.0 1.4203745434043815 2.9031671812046635E-4 41 0.0 8.709501543613991E-5 0.0 1.5098501559291093 2.9031671812046635E-4 42 0.0 1.1612668724818653E-4 0.0 1.6069320664685933 2.9031671812046635E-4 43 0.0 1.1612668724818653E-4 0.0 1.7089203295443132 2.9031671812046635E-4 44 0.0 1.1612668724818653E-4 0.0 1.8196180941636468 2.9031671812046635E-4 45 0.0 1.1612668724818653E-4 0.0 1.9326093608561323 3.19348389932513E-4 46 0.0 1.1612668724818653E-4 0.0 2.0532940205788104 3.4838006174455963E-4 47 0.0 1.1612668724818653E-4 0.0 2.177259259216249 3.4838006174455963E-4 48 0.0 1.1612668724818653E-4 0.0 2.307030832216098 3.4838006174455963E-4 49 0.0 1.1612668724818653E-4 0.0 2.435234694938096 3.7741173355660627E-4 50 0.0 1.1612668724818653E-4 0.0 2.564919172922508 3.7741173355660627E-4 51 0.0 1.1612668724818653E-4 0.0 2.7042131342767077 3.7741173355660627E-4 52 0.0 1.4515835906023317E-4 0.0 2.848036036433587 3.7741173355660627E-4 53 0.0 1.4515835906023317E-4 0.0 3.0038780507206533 3.7741173355660627E-4 54 0.0 1.4515835906023317E-4 0.0 3.1715359554352225 3.7741173355660627E-4 55 0.0 1.4515835906023317E-4 0.0 3.3428808824699217 3.7741173355660627E-4 56 0.0 1.4515835906023317E-4 0.0 3.524735274700582 3.7741173355660627E-4 57 0.0 1.7419003087227981E-4 0.0 3.711554082811102 4.354750771806995E-4 58 0.0 1.7419003087227981E-4 0.0 3.9120758400169082 4.354750771806995E-4 59 0.0 1.7419003087227981E-4 0.0 4.1001139783435345 4.354750771806995E-4 60 0.0 1.7419003087227981E-4 0.0 4.297326124962767 4.354750771806995E-4 61 0.0 1.7419003087227981E-4 0.0 4.509605709252452 4.354750771806995E-4 62 0.0 1.7419003087227981E-4 0.0 4.718372461252879 4.354750771806995E-4 63 0.0 1.7419003087227981E-4 0.0 4.933090705974776 4.354750771806995E-4 64 0.0 1.7419003087227981E-4 0.0 5.168566596042286 4.354750771806995E-4 65 0.0 1.7419003087227981E-4 0.0 5.409848820472206 4.354750771806995E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAA 240 1.8189894E-11 20.71043 59 TCGCGGT 295 0.0 20.453459 33 TAACGCG 95 0.006642126 18.686375 52 ATTCGCG 275 1.3096724E-10 18.072458 56 CTTCGCG 415 0.0 17.1047 31 CGATCGT 315 5.0931703E-11 16.902054 1 CGCGGTT 380 0.0 16.812365 34 GACGCGA 580 0.0 15.922341 69 TCGCGCT 360 2.1827873E-11 15.776169 37 CTCGCGC 360 2.1827873E-11 15.775709 36 CGGTTAC 405 9.094947E-12 14.899064 4 TCGCGGA 340 2.6684575E-9 14.619127 58 CCGGTTA 250 4.5610723E-6 14.196902 31 TAAGGCG 1140 0.0 14.011118 17 TTAGTCG 230 3.2713826E-5 13.889685 15 GGACGCG 665 0.0 13.88554 68 CGTGCGA 155 0.0067114676 13.743726 50 CCTCGCG 390 1.2587407E-9 13.651066 35 CGCGACC 655 0.0 13.558671 71 ACGCGAC 685 0.0 13.482474 70 >>END_MODULE