FastQCFastQC Report
Thu 26 May 2016
SRR936112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936112_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3444514
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60580.1758738678373785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44500.12919093956360753No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13750.039.825558100-101
GGTATCA71400.037.2585681
CGGTATC10150.032.253246100-101
GTATCAA139600.030.0978971
CGTACAT18850.029.997772100-101
CGAGTAC12800.028.598412100-101
CCTGTCG10900.028.39143896-97
GCGTACA6100.027.321526100-101
TTATACG9050.027.29041196-97
TCGTATC7150.026.63917100-101
TCGTACA9550.026.17717100-101
CGGGCTA3450.025.016449100-101
CGCGCGA600.001286898224.79700996-97
CCGTACA8900.024.410666100-101
GCGTATC4650.023.680754100-101
AGTATCA35800.023.609245100-101
ATCAACG175850.023.4466933
TCAACGC176200.023.4001184
CCGTATC6550.023.172647100-101
TATCAAC179050.023.1283442
Produced by FastQC (version 0.11.5)