FastQCFastQC Report
Thu 26 May 2016
SRR936111_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936111_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086358
Sequences flagged as poor quality0
Sequence length77
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143050.6856445538109951No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48440.23217491916535898No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAT604.5896767E-429.58188426
TTCGCGA700.001129828325.35590225
CGGTATA1901.8189894E-1124.28420810
ATTCGCG2200.024.20510156
CGGTTAC3350.023.3067044
CGATCGT2550.022.2686141
CGCGGAA2301.0913936E-1121.60660259
GCGTAAG3450.020.57475738
TGCGTAA3500.020.28180537
CGCTCCG3001.8189894E-1218.93240725
CGTAAGT3600.018.7329539
AACGGGC950.006654030618.6801630
CGCGTCA950.00665679318.6788162
AATTCGC3051.8189894E-1218.62337955
TTGCGTA3650.018.4758936
GCGGTAT2751.3096724E-1018.0683779
ATTTGCG3151.8189894E-1218.026113
TCGCGCT3203.6379788E-1217.74657837
TTGCGCG3400.017.7448775
CAATTCG3551.8189894E-1217.00120554
Produced by FastQC (version 0.11.5)