##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936111_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2086358 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6264164635216 39.0 36.0 41.0 30.0 41.0 2 36.480295807335075 39.0 36.0 41.0 30.0 41.0 3 36.38384831366429 39.0 36.0 41.0 30.0 41.0 4 36.2590576497418 39.0 36.0 41.0 29.0 41.0 5 36.174137420327675 39.0 35.0 41.0 29.0 41.0 6 36.04745398440728 39.0 35.0 40.0 29.0 41.0 7 35.84108479944477 39.0 35.0 40.0 28.0 41.0 8 35.625288181606415 38.0 35.0 40.0 27.0 41.0 9 35.50336902870936 38.0 35.0 40.0 27.0 41.0 10-11 35.672372382879644 39.0 35.0 40.0 28.0 41.0 12-13 35.79088847647432 39.0 35.0 41.0 28.5 41.0 14-15 35.694134228162184 39.0 35.0 41.0 27.5 41.0 16-17 35.47628594900779 39.0 35.0 41.0 26.5 41.0 18-19 35.250942551565934 39.0 35.0 41.0 26.0 41.0 20-21 34.93096534727022 39.0 34.5 41.0 24.5 41.0 22-23 34.522283567824886 39.0 34.0 41.0 22.5 41.0 24-25 34.18365975542069 38.0 33.5 40.5 20.5 41.0 26-27 33.21875560186699 37.5 32.0 39.5 16.5 40.5 28-29 33.231657510360165 37.5 33.0 39.5 16.0 40.5 30-31 33.58131250724948 38.0 33.0 40.0 15.5 41.0 32-33 33.41129901963134 38.0 33.0 40.0 13.5 41.0 34-35 33.133277462448916 37.5 33.0 40.0 9.5 41.0 36-37 32.83541295405678 37.0 32.0 40.0 8.0 41.0 38-39 32.5511177851548 36.5 32.0 40.0 7.0 41.0 40-41 32.161760110201605 36.0 31.5 39.5 2.0 41.0 42-43 31.794590621552004 35.5 31.0 39.0 2.0 41.0 44-45 31.414137458672002 35.0 31.0 39.0 2.0 40.5 46-47 31.02421348589264 35.0 31.0 38.0 2.0 40.0 48-49 30.558493796366683 35.0 30.0 37.0 2.0 40.0 50-51 30.119317729747245 35.0 30.0 37.0 2.0 39.0 52-53 29.667370365009262 34.5 29.0 36.0 2.0 39.0 54-55 29.209314269171443 34.0 29.0 36.0 2.0 38.5 56-57 28.759854013548967 34.0 29.0 35.0 2.0 37.0 58-59 28.3270816897196 34.0 28.5 35.0 2.0 37.0 60-61 27.974440628118472 34.0 27.0 35.0 2.0 36.0 62-63 27.62471948725962 34.0 27.0 35.0 2.0 36.0 64-65 27.240215964853586 34.0 26.0 35.0 2.0 35.5 66-67 26.842597243617824 33.0 25.5 35.0 2.0 35.0 68-69 26.473786617637053 33.0 25.0 35.0 2.0 35.0 70-71 26.039465901825096 33.0 24.0 35.0 2.0 35.0 72-73 25.55014024438759 33.0 21.5 35.0 2.0 35.0 74-75 24.965479558158286 33.0 10.0 35.0 2.0 35.0 76-77 23.311475307689285 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9040289968459874 1101 2 0.8706685102885672 1101 3 0.9081740047993492 1101 4 0.840610261196538 1101 5 1.0582129051627618 1101 6 0.9782876159387826 1101 7 1.159057198822886 1101 8 1.2912751970239995 1101 9 1.285959816831955 1101 10-11 1.0285596506956196 1101 12-13 1.1161985283347704 1101 14-15 1.2777749298745533 1101 16-17 1.205941276358061 1101 18-19 1.2550489529020936 1101 20-21 1.237023780237699 1101 22-23 1.1947414159040193 1101 24-25 1.1922441782022943 1101 26-27 0.9940717529404779 1101 28-29 1.0262985491561167 1101 30-31 1.0926334671731155 1101 32-33 1.13280166825853 1101 34-35 1.0199863208363666 1101 36-37 0.9501431345501032 1101 38-39 1.168461605452805 1101 40-41 0.987573460207372 1101 42-43 0.9188411029056631 1101 44-45 1.0109060440503654 1101 46-47 1.032197670296842 1101 48-49 1.0189645223430581 1101 50-51 0.9164990183641031 1101 52-53 0.9630709874955912 1101 54-55 0.8790599699921202 1101 56-57 0.816260125059511 1101 58-59 0.8270337142695219 1101 60-61 0.7686073318774511 1101 62-63 0.8429879109361345 1101 64-65 0.6154303898451303 1101 66-67 0.6875167828029163 1101 68-69 0.6428949943990858 1101 70-71 0.5799740053134492 1101 72-73 0.4843431703059551 1101 74-75 0.444563243275784 1101 76-77 0.27435413437873635 1102 1 0.7685758582498394 1102 2 1.0241729737720817 1102 3 0.8698159263679344 1102 4 0.8707171299623937 1102 5 1.0618881866012728 1102 6 0.9855652122682343 1102 7 0.746081241844827 1102 8 0.9465447430740568 1102 9 0.8246764659467161 1102 10-11 0.9393813248095952 1102 12-13 0.9621654657762946 1102 14-15 1.1128526991456127 1102 16-17 1.1906512452220426 1102 18-19 1.1959924702599594 1102 20-21 1.2475389948519222 1102 22-23 1.2672570955438474 1102 24-25 1.2983538741496048 1102 26-27 1.1243083279125088 1102 28-29 1.2707439707230677 1102 30-31 1.3639940155766226 1102 32-33 1.4080009921744576 1102 34-35 1.6561335945556692 1102 36-37 1.5659835185937325 1102 38-39 1.5800205684130262 1102 40-41 1.506776866982804 1102 42-43 1.4529136218524634 1102 44-45 1.3764111359174294 1102 46-47 1.1724706849805422 1102 48-49 1.3451428231808684 1102 50-51 1.3621187929519252 1102 52-53 1.1584406883000717 1102 54-55 1.277016933886749 1102 56-57 1.3234810906356351 1102 58-59 1.3465214999961752 1102 60-61 1.3888257087747178 1102 62-63 1.4112207537237218 1102 64-65 1.345522501943531 1102 66-67 1.1250926761802802 1102 68-69 1.1735997532337556 1102 70-71 1.3895228760199956 1102 72-73 1.4274341639469732 1102 74-75 1.4671928106277186 1102 76-77 1.5270521269274369 1103 1 0.45651475720264045 1103 2 0.33087259274653036 1103 3 0.47488417605790545 1103 4 0.5798269682539114 1103 5 0.5971191398713387 1103 6 0.5972984665645384 1103 7 0.5860863564253407 1103 8 0.6014523328761427 1103 9 0.444809990737987 1103 10-11 0.6139365810040687 1103 12-13 0.725283214547666 1103 14-15 0.8821478018812741 1103 16-17 0.844516926493796 1103 18-19 0.9462914003710381 1103 20-21 0.9187483737941875 1103 22-23 0.8136189489051873 1103 24-25 0.8356400220893789 1103 26-27 0.608381438593959 1103 28-29 0.6265950561804416 1103 30-31 0.9000385669968836 1103 32-33 0.8770438701210921 1103 34-35 0.8440113699913425 1103 36-37 0.8214182953872395 1103 38-39 0.8764232153689449 1103 40-41 0.7789170888785577 1103 42-43 0.7508324530317232 1103 44-45 0.7960452133615945 1103 46-47 0.5370046536061217 1103 48-49 0.5787270794748522 1103 50-51 0.6611949584717358 1103 52-53 0.7011497093287034 1103 54-55 0.7755345882844864 1103 56-57 0.7698668049060657 1103 58-59 0.8670170044083072 1103 60-61 1.0068885239553929 1103 62-63 0.9456648587416829 1103 64-65 0.930328506332657 1103 66-67 0.996316393067918 1103 68-69 0.9331705992688306 1103 70-71 0.9142491443129153 1103 72-73 0.9440014458256307 1103 74-75 1.0007078916852592 1103 76-77 0.9725696732555846 1104 1 0.6180507494773124 1104 2 0.5991777961995837 1104 3 0.6046059900239769 1104 4 0.6837612605322931 1104 5 0.6694915472974188 1104 6 0.5215544528966518 1104 7 0.735258642877902 1104 8 0.7503094514779391 1104 9 0.6267576113268305 1104 10-11 0.7121179988349269 1104 12-13 0.7163652643059848 1104 14-15 0.7011556333248379 1104 16-17 0.6682794682110824 1104 18-19 0.6760329786509871 1104 20-21 0.5881808063700191 1104 22-23 0.643079051520445 1104 24-25 0.6305880512291395 1104 26-27 0.42803499272164913 1104 28-29 0.36795500015059446 1104 30-31 0.5374626269318341 1104 32-33 0.5597943993802446 1104 34-35 0.5543632278368449 1104 36-37 0.44623192719266314 1104 38-39 0.47305001737031205 1104 40-41 0.598239027001668 1104 42-43 0.553041148268651 1104 44-45 0.5325447278757593 1104 46-47 0.3884672128327402 1104 48-49 0.4921630988093888 1104 50-51 0.46426691649180185 1104 52-53 0.6523022739142341 1104 54-55 0.6544292192887227 1104 56-57 0.6560431927853649 1104 58-59 0.5575948651932556 1104 60-61 0.5131023446492939 1104 62-63 0.5904339478853693 1104 64-65 0.5195580164429643 1104 66-67 0.6654644317582203 1104 68-69 0.5550034011322502 1104 70-71 0.5289338968743174 1104 72-73 0.37373214710062896 1104 74-75 0.3696050960296944 1104 76-77 0.47522764185553257 1105 1 0.3105778336190639 1105 2 0.6481363807975455 1105 3 0.6343980215705756 1105 4 0.5792253878094087 1105 5 0.3308836669395774 1105 6 0.07808820713100317 1105 7 -0.0012428096501295727 1105 8 0.5088777937082298 1105 9 0.22617607573727128 1105 10-11 0.4845105274922048 1105 12-13 0.5673345776849814 1105 14-15 0.24538458739978353 1105 16-17 0.3638820313724551 1105 18-19 0.526984987544445 1105 20-21 0.6180404827237069 1105 22-23 0.639744791785354 1105 24-25 0.6191969803436805 1105 26-27 0.5345527534163494 1105 28-29 0.6906878503566745 1105 30-31 0.6132699074008343 1105 32-33 0.7794127043796593 1105 34-35 0.7829059279658424 1105 36-37 0.7202510774899835 1105 38-39 0.8094534111469898 1105 40-41 0.8181431182015473 1105 42-43 0.5643197399981581 1105 44-45 0.42310382007196523 1105 46-47 0.35410207770043556 1105 48-49 0.49361090743226654 1105 50-51 0.5892941841659756 1105 52-53 0.5358517385239416 1105 54-55 0.24530765789975106 1105 56-57 0.4039477769008961 1105 58-59 0.46847781345808315 1105 60-61 0.2014865776410808 1105 62-63 0.24057893849899514 1105 64-65 0.4968899269630427 1105 66-67 0.5231245506872284 1105 68-69 0.350569782187506 1105 70-71 -0.04069043847612619 1105 72-73 -0.12841020064611897 1105 74-75 -0.227537828533233 1105 76-77 0.04181372904916003 1106 1 -0.1864994360480665 1106 2 0.13863641597404097 1106 3 -0.43107108653321546 1106 4 -0.28455735393399806 1106 5 -0.40915612101547083 1106 6 -0.2729945356908061 1106 7 -0.11338881727186845 1106 8 -0.07240712923470483 1106 9 -0.029054823296938537 1106 10-11 -0.3185779945454854 1106 12-13 -0.19875661519878918 1106 14-15 -0.040241115167276575 1106 16-17 -2.2050837056042383E-4 1106 18-19 -0.16367816900432075 1106 20-21 -0.2491901975486499 1106 22-23 -0.15412440710974096 1106 24-25 -0.1542756279498647 1106 26-27 -0.2072604948223855 1106 28-29 -0.0905604325424676 1106 30-31 -0.19880068900253178 1106 32-33 -0.3181217056796797 1106 34-35 -0.304926701751981 1106 36-37 -0.09255796856965759 1106 38-39 -0.2532802750468335 1106 40-41 -0.18463890614674483 1106 42-43 -0.05739992335507438 1106 44-45 -0.19499369122944898 1106 46-47 -0.21982184247099568 1106 48-49 -0.1432152636754509 1106 50-51 -0.2225278658996821 1106 52-53 -0.272174716255261 1106 54-55 -0.24400305223924335 1106 56-57 -0.2762249525333047 1106 58-59 -0.35591752311422553 1106 60-61 -0.2809430192978972 1106 62-63 -0.29989752585745677 1106 64-65 -0.2842936517242123 1106 66-67 -0.36185177321999973 1106 68-69 -0.32713802488918375 1106 70-71 -0.5574082608404716 1106 72-73 -0.49605362703197997 1106 74-75 -0.3458514056850497 1106 76-77 -0.22185156941441875 1107 1 0.2823806968954443 1107 2 0.05842685038264506 1107 3 0.1794879207533029 1107 4 0.04038753530159056 1107 5 0.08977925748597215 1107 6 -0.05492236006647033 1107 7 0.12967036747217264 1107 8 0.08965821421301001 1107 9 0.18204964019886916 1107 10-11 0.2431622615523139 1107 12-13 0.17824429570863032 1107 14-15 0.2026983630358714 1107 16-17 0.27523153840105863 1107 18-19 0.3368730004717051 1107 20-21 0.12665396234268655 1107 22-23 -0.030649336120546877 1107 24-25 -0.21237880855760238 1107 26-27 -0.17351203041739893 1107 28-29 -0.15657537458989879 1107 30-31 0.19530848781465693 1107 32-33 0.2984826055860097 1107 34-35 0.21530073572917985 1107 36-37 0.24015622142886173 1107 38-39 0.16595651661012312 1107 40-41 0.2517266796077777 1107 42-43 0.26709619001592344 1107 44-45 0.338706049645463 1107 46-47 0.3187192118023141 1107 48-49 0.42072348713732666 1107 50-51 0.21639001664989976 1107 52-53 0.2994470180006701 1107 54-55 0.24794235465293468 1107 56-57 0.2987979067167821 1107 58-59 0.1962407685052554 1107 60-61 0.11331158612683723 1107 62-63 0.08015788046339622 1107 64-65 0.22931356885561627 1107 66-67 0.2312624790061335 1107 68-69 0.13930283995594905 1107 70-71 0.3022345833176878 1107 72-73 0.2787391681182214 1107 74-75 0.31660916720968046 1107 76-77 0.3440651835901747 1108 1 -0.06932397964999382 1108 2 -0.02404882444972145 1108 3 -0.15984460329259775 1108 4 -0.2038869446416811 1108 5 -0.058923184210101454 1108 6 -0.2190714923684709 1108 7 -0.4221894264258381 1108 8 -0.23331793678862311 1108 9 -0.28151039024013613 1108 10-11 -0.35675209632382376 1108 12-13 -0.17444229583146154 1108 14-15 -0.1652349539420399 1108 16-17 -0.17247986790907532 1108 18-19 -0.226470827203336 1108 20-21 -0.1297610074480815 1108 22-23 -0.09823826995151563 1108 24-25 0.05135462715035288 1108 26-27 -0.23439453079034678 1108 28-29 -0.0711614961823912 1108 30-31 -0.055543278960669795 1108 32-33 -0.10177601760254618 1108 34-35 0.11277292619499946 1108 36-37 0.12110907371928903 1108 38-39 -0.02665766326520469 1108 40-41 0.18593967114561138 1108 42-43 -0.0011704572316126871 1108 44-45 -0.15113001662372483 1108 46-47 -0.11248734997749565 1108 48-49 -0.06179870876066573 1108 50-51 -0.057901627068492445 1108 52-53 -0.07014941529002883 1108 54-55 -0.0272647453949304 1108 56-57 -0.08836282120827477 1108 58-59 -0.22758982411514594 1108 60-61 -0.26967944973043956 1108 62-63 -0.16614794864497995 1108 64-65 -0.14371573729470555 1108 66-67 -0.15063222454462277 1108 68-69 -0.2914352118012111 1108 70-71 -0.33225159575447805 1108 72-73 -0.1733864368736242 1108 74-75 -0.28562181718691093 1108 76-77 -0.22725050237519184 1109 1 -0.06323083532155493 1109 2 -0.12315897355986749 1109 3 -0.4236535612192043 1109 4 -0.08885850902500891 1109 5 -0.22331217212850163 1109 6 -0.215690341928493 1109 7 -0.1801294711305914 1109 8 -0.1467392649158299 1109 9 -0.09014151945950033 1109 10-11 -0.19215479275593594 1109 12-13 -0.10802072720401412 1109 14-15 -0.013749864956942304 1109 16-17 0.09326936107544981 1109 18-19 -0.06453706379898705 1109 20-21 0.02670668063725401 1109 22-23 -0.12058074229398841 1109 24-25 -0.2621247039760348 1109 26-27 -0.3791031718225142 1109 28-29 -0.2263647896209804 1109 30-31 -0.22070646544739247 1109 32-33 -0.2202591684386377 1109 34-35 -0.15043513480718218 1109 36-37 0.03335796317406192 1109 38-39 -0.07165384355550941 1109 40-41 -0.1068676032793121 1109 42-43 0.037493846873868364 1109 44-45 -0.07388593717376324 1109 46-47 -0.23427384235221993 1109 48-49 -0.07019287450777156 1109 50-51 0.09549543882857492 1109 52-53 -0.05520480108070913 1109 54-55 0.1396045780920403 1109 56-57 0.06183205472189357 1109 58-59 -0.1944114012308411 1109 60-61 -0.2400586620214149 1109 62-63 -0.15452259143139102 1109 64-65 -0.1280390120591619 1109 66-67 -0.1179447050923983 1109 68-69 -0.22213931162295708 1109 70-71 -0.12135162970745128 1109 72-73 -0.09288145710393891 1109 74-75 -0.1508568000689543 1109 76-77 -0.017328452747261025 1110 1 -0.3922374450176207 1110 2 -0.3917767450690377 1110 3 -0.5280148543965595 1110 4 -0.3526890906229525 1110 5 -0.17972689598700953 1110 6 -0.5818923939763962 1110 7 -0.6247801804975524 1110 8 -0.6472605735803398 1110 9 -0.47135090024990234 1110 10-11 -0.5036529674842214 1110 12-13 -0.4577051073030205 1110 14-15 -0.5696678245244087 1110 16-17 -0.6455750483385287 1110 18-19 -0.5548140349541555 1110 20-21 -0.7455834131153409 1110 22-23 -0.8357429425760827 1110 24-25 -0.8271320327185308 1110 26-27 -0.7240950825501358 1110 28-29 -0.4906821103433501 1110 30-31 -0.4804491989300601 1110 32-33 -0.40247797979957767 1110 34-35 -0.36570484635893763 1110 36-37 -0.2748007939818322 1110 38-39 -0.28967978453838583 1110 40-41 -0.3036184122065535 1110 42-43 -0.3354261038991062 1110 44-45 -0.3422351033564546 1110 46-47 -0.30088519775474865 1110 48-49 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-0.8182983718667671 2314 14-15 -0.7066639857987624 2314 16-17 -1.2355723385371888 2314 18-19 -0.8994268445585405 2314 20-21 -0.8533964502281322 2314 22-23 -0.659990110929435 2314 24-25 -0.5615057191112172 2314 26-27 -0.5877464967696042 2314 28-29 -0.7157002794177103 2314 30-31 -0.7521094645288926 2314 32-33 -0.842636469664626 2314 34-35 -0.8627494393477377 2314 36-37 -1.1414987717806113 2314 38-39 -0.97224886771588 2314 40-41 -0.8530034405062601 2314 42-43 -0.6671380568041023 2314 44-45 -0.8192620203972751 2314 46-47 -0.7848189483783408 2314 48-49 -0.6968641336427908 2314 50-51 -0.6179380302820867 2314 52-53 -0.571127188365562 2314 54-55 -0.553380426500425 2314 56-57 -0.515711274218642 2314 58-59 -0.6156954953165403 2314 60-61 -0.4696306639382719 2314 62-63 -0.5417405539728186 2314 64-65 -0.3873073120471773 2314 66-67 -0.47630599258091166 2314 68-69 -0.34661017939980354 2314 70-71 -0.4645792364324244 2314 72-73 -0.4257760100371293 2314 74-75 -0.38927379619530456 2314 76-77 -0.3190266676747804 2315 1 -1.406693245411219 2315 2 -1.1999596435766193 2315 3 -1.259041284147571 2315 4 -1.085370480647832 2315 5 -1.0504379973329492 2315 6 -1.1548222761092006 2315 7 -1.1868665880879234 2315 8 -1.1733034584324358 2315 9 -1.176962881170013 2315 10-11 -1.1537236665365995 2315 12-13 -1.120422195328949 2315 14-15 -1.2896083239184222 2315 16-17 -1.293702261744535 2315 18-19 -1.1898622189866828 2315 20-21 -1.2093251944243306 2315 22-23 -1.165220608290646 2315 24-25 -1.270325019278033 2315 26-27 -1.225707517078206 2315 28-29 -1.1023328652934623 2315 30-31 -1.2508116482240155 2315 32-33 -1.3043536605068269 2315 34-35 -1.2988918793140272 2315 36-37 -1.502368463074827 2315 38-39 -1.3262454289333725 2315 40-41 -1.2802159627235916 2315 42-43 -1.4021221251430802 2315 44-45 -1.301949046839738 2315 46-47 -1.296346460344143 2315 48-49 -1.2405520231923681 2315 50-51 -1.1603462697141431 2315 52-53 -1.1453060425823374 2315 54-55 -1.2519968112073983 2315 56-57 -1.2125558160042864 2315 58-59 -1.1675121993390292 2315 60-61 -1.2917423254365303 2315 62-63 -1.5081534910274108 2315 64-65 -1.5017396229567836 2315 66-67 -1.2956296986878257 2315 68-69 -1.27163926235702 2315 70-71 -1.3203749324959055 2315 72-73 -2.482438309995853 2315 74-75 -2.9912597954379514 2315 76-77 -2.6547508588956497 2316 1 -3.6049795119785344 2316 2 -3.5687546643076473 2316 3 -2.9309002621809555 2316 4 -2.818119300794187 2316 5 -3.596765165493146 2316 6 -3.1609579927150477 2316 7 -3.46706192472039 2316 8 -3.2935147812059995 2316 9 -2.913478953292355 2316 10-11 -2.7613777480977646 2316 12-13 -2.8922021183916726 2316 14-15 -2.9539431475931934 2316 16-17 -4.209664359941478 2316 18-19 -4.076757484286571 2316 20-21 -3.43930390610695 2316 22-23 -3.7671111605449425 2316 24-25 -3.5929194553366557 2316 26-27 -3.3807014347018125 2316 28-29 -3.5009976085280137 2316 30-31 -3.6270575800377074 2316 32-33 -3.753944771957613 2316 34-35 -3.903580404321829 2316 36-37 -3.811786672627022 2316 38-39 -3.9254486694706614 2316 40-41 -3.908189082441318 2316 42-43 -4.02544509296062 2316 44-45 -3.8175096604273193 2316 46-47 -3.399832442218024 2316 48-49 -3.5880356389899895 2316 50-51 -4.066465904644179 2316 52-53 -5.047581599708906 2316 54-55 -4.1904744497168025 2316 56-57 -4.15897238872849 2316 58-59 -4.324853882405822 2316 60-61 -3.9151369442848925 2316 62-63 -3.96136576596356 2316 64-65 -3.9843491216543256 2316 66-67 -3.8699222641768642 2316 68-69 -3.913194631089148 2316 70-71 -3.747052442351901 2316 72-73 -3.7660884204976632 2316 74-75 -3.7536255691735505 2316 76-77 -3.687156231767613 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50245.0 3 5343.0 4 7733.0 5 9223.0 6 10421.0 7 10662.0 8 13392.0 9 14866.0 10 20168.0 11 23056.0 12 15904.0 13 11533.0 14 11111.0 15 11310.0 16 11322.0 17 11885.0 18 12272.0 19 12761.0 20 13707.0 21 14899.0 22 16482.0 23 18592.0 24 21608.0 25 24875.0 26 29674.0 27 34630.0 28 40304.0 29 47435.0 30 56143.0 31 66857.0 32 81324.0 33 102920.0 34 135787.0 35 179952.0 36 246792.0 37 327450.0 38 287774.0 39 75401.0 40 545.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.711207183749227 26.34514342907735 30.584669484932313 21.35897990224111 2 21.525798044072367 26.71992153663182 30.487438334973582 21.266842084322235 3 21.712946495768616 26.781453042503685 30.02596259583774 21.479637865889963 4 21.483021881672336 26.832684735369156 30.266035422173516 21.418257960784988 5 21.67483741199491 26.869552337111198 30.2393795890036 21.216230661890293 6 21.783595386157415 26.544215032713264 30.420912711747008 21.251276869382313 7 21.566544488110843 26.52752756687622 30.33295973476739 21.57296821024555 8 21.494449864212832 26.811841887645404 30.028068274699958 21.665639973441802 9 21.536170063849685 26.71936099497937 30.08528791803232 21.659181023138622 10-11 21.857176757665027 26.769554180739092 29.919220876968843 21.454048184627037 12-13 21.975675104301942 26.4788153082606 29.777527539404154 21.767982048033303 14-15 21.690889302565925 26.783119388486597 29.941205612106597 21.584785696840882 16-17 21.81524697209184 26.730985623502825 29.91469010302138 21.539077301383962 18-19 21.81826505864683 26.719770887375173 29.532623093145077 21.92934096083292 20-21 21.878276527799816 26.86471137310815 29.430752510116935 21.826259588975095 22-23 22.051371909527102 27.135300647464305 28.79270954858236 22.020617894426238 24-25 22.09918132714459 27.28915111050813 28.43302554199863 22.178642020348647 26-27 22.11990547706611 27.376269450768824 28.265981667843647 22.23784340432142 28-29 22.240067339018257 27.511689536830943 28.117611276792942 22.13063184735786 30-31 22.0016086842104 27.411336621964484 28.225361510552556 22.36169318327256 32-33 22.13499005216114 27.645189193490438 27.94167098286949 22.27814977147893 34-35 22.06261330919905 27.280924363383264 28.269105213253788 22.3873571141639 36-37 22.013980516437833 27.493993027717757 28.451129909988293 22.040896545856118 38-39 22.05083070397716 27.345807555096634 28.421662443398134 22.18169929752807 40-41 21.899551486532253 27.273971430605286 28.512470678129446 22.31400640473301 42-43 21.857606792987745 27.552385903677962 28.122678758514663 22.46732854481963 44-45 21.907966627014535 27.2504102833166 28.39709432885032 22.44452876081855 46-47 21.74026542488813 27.413055406600407 28.440825946369912 22.405853222141545 48-49 21.944586604853853 27.29775501080486 28.215143099933243 22.542515284408047 50-51 21.678764446552044 27.596651379918875 28.09859916363613 22.62598500989295 52-53 21.660564652302657 27.455252627230653 28.247191878642834 22.63699084182385 54-55 21.576666601941703 27.42207413789342 28.239646342787733 22.761612917377143 56-57 21.56785522607622 27.62581436650502 28.099470708214508 22.70685969920425 58-59 21.62488794289522 27.677817247446058 27.908019693287123 22.789275116371602 60-61 21.560259445579973 27.309022754822298 27.96125622899747 23.16946157060026 62-63 21.480758065436337 27.257413642742318 28.007707417310428 23.254120874510914 64-65 21.55159394706196 27.099784878394207 28.078547432947694 23.270073741596136 66-67 21.308856921489586 27.265339429342927 28.154186339977834 23.271617309189658 68-69 21.46863288685235 26.93916176146566 28.26887062856502 23.323334723116975 70-71 21.201131698960786 26.84200215472643 28.39848387444079 23.558382271872 72-73 21.391886602013287 26.561942326216858 28.28231197878489 23.76385909298497 74-75 21.22356912145168 26.56675978069033 28.217602819997307 23.99206827786069 76-77 21.056294107205396 26.429637163513895 28.56736438608527 23.94670434319544 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14712.0 1 8813.0 2 2743.5 3 2593.5 4 2614.0 5 2791.0 6 3002.5 7 3114.0 8 3289.0 9 3387.0 10 3508.5 11 3671.0 12 3856.0 13 4000.0 14 4265.0 15 4764.5 16 5329.0 17 5659.0 18 6049.5 19 6896.5 20 7890.0 21 9489.0 22 10551.0 23 11660.0 24 14011.5 25 17360.0 26 19466.0 27 21922.0 28 27687.0 29 33265.0 30 35534.0 31 39846.5 32 48927.5 33 57069.5 34 62466.0 35 64489.0 36 68012.5 37 74260.0 38 77759.0 39 78534.0 40 79977.5 41 82171.5 42 85082.0 43 87242.0 44 87317.5 45 88323.5 46 89593.0 47 90109.0 48 90286.0 49 90718.5 50 91328.0 51 89279.5 52 87054.0 53 85204.0 54 79343.0 55 71484.0 56 63613.5 57 59591.0 58 56993.0 59 49413.0 60 41227.5 61 38024.0 62 33696.0 63 24538.5 64 16924.0 65 14139.0 66 12507.0 67 9711.0 68 7243.0 69 5076.0 70 4213.0 71 3469.5 72 2317.5 73 1693.5 74 1478.0 75 1345.5 76 964.0 77 568.5 78 422.0 79 343.5 80 235.0 81 167.5 82 110.5 83 91.0 84 81.5 85 69.5 86 60.5 87 54.0 88 43.5 89 39.5 90 33.5 91 21.0 92 27.5 93 30.0 94 25.0 95 21.5 96 19.0 97 24.5 98 30.0 99 65.0 100 100.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009825734605470393 2 0.013851889273077775 3 0.01342051555869127 4 0.015337732067075737 5 0.0123181160663702 6 0.009634012954631947 7 0.01629634032126797 8 0.017878043940685154 9 0.017494600639008262 10-11 0.02027456457616574 12-13 0.01663185321023525 14-15 0.009082813208471412 16-17 0.006734222985700441 18-19 0.01902837384571584 20-21 0.023917275942096226 22-23 0.021640581338389673 24-25 0.020178703750746516 26-27 0.024684162545450014 28-29 0.009466256510148307 30-31 0.008675404700439714 32-33 0.010856238478727045 34-35 0.012078464002822142 36-37 0.01152726425666161 38-39 0.014187402162045058 40-41 0.011718985907500055 42-43 0.015553418924268989 44-45 0.014355158606528697 46-47 0.02552294476786822 48-49 0.023509867434064528 50-51 0.03750554794527114 52-53 0.040477233533267064 54-55 0.029213586546508317 56-57 0.021928163814647343 58-59 0.0176383918771371 60-61 0.021496790100260836 62-63 0.027559987308026716 64-65 0.02688896153009215 66-67 0.029812716705378463 68-69 0.043209267057714926 70-71 0.056294269727438916 72-73 0.08656232535355869 74-75 0.08708955989336442 76-77 0.09336844395832354 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2086358.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.952933726190125 #Duplication Level Percentage of deduplicated Percentage of total 1 76.23267951917367 41.12976705867869 2 12.237131693400444 13.204583105053896 3 4.609562808959222 7.460983102154629 4 2.235016703039171 4.823428322240014 5 1.2969272252609905 3.498651431609893 6 0.7679915455311719 2.4861238174990583 7 0.5222836190998239 1.9725113441297264 8 0.34990791460353604 1.5102846821499178 9 0.2641210136048318 1.2825093188444083 >10 1.337410098824557 12.893386678218807 >50 0.09634060261602621 3.567755886314857 >100 0.04857819413570836 4.572477884952836 >500 0.0017817928269101677 0.6251525758274653 >1k 1.7817928269101533E-4 0.28479386527585016 >5k 0.0 0.0 >10k+ 8.908964134550767E-5 0.6875909270499639 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14305 0.6856445538109951 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4844 0.23217491916535898 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.8758247625767006E-4 0.0 3 0.0 0.0 0.0 6.230953652249518E-4 0.0 4 9.586082541922335E-5 0.0 0.0 0.0017254948575460205 0.0 5 2.396520635480584E-4 0.0 0.0 0.004601319620122721 0.0 6 2.396520635480584E-4 0.0 0.0 0.009586082541922336 0.0 7 2.396520635480584E-4 0.0 0.0 0.018213556829652436 0.0 8 2.396520635480584E-4 0.0 0.0 0.02712861359364021 0.0 9 2.396520635480584E-4 0.0 0.0 0.038823634294785456 0.0 10 2.396520635480584E-4 0.0 0.0 0.05387378388560352 0.0 11 2.396520635480584E-4 0.0 0.0 0.06863635100016392 0.0 12 2.396520635480584E-4 0.0 0.0 0.0898695238305219 0.0 13 2.396520635480584E-4 0.0 0.0 0.11661469412248521 0.0 14 2.8758247625767006E-4 0.0 0.0 0.1498304701302461 0.0 15 2.8758247625767006E-4 0.0 0.0 0.18688067915477594 0.0 16 2.8758247625767006E-4 0.0 0.0 0.22354744487762887 0.0 17 2.8758247625767006E-4 0.0 0.0 0.25848871574293575 0.0 18 3.355128889672817E-4 0.0 0.0 0.2955868551801752 0.0 19 3.834433016768934E-4 0.0 0.0 0.33278085544283387 0.0 20 3.834433016768934E-4 0.0 0.0 0.3717962113884578 0.0 21 3.834433016768934E-4 0.0 0.0 0.4182407813040715 0.0 22 3.834433016768934E-4 0.0 0.0 0.4707725136338059 0.0 23 3.834433016768934E-4 0.0 0.0 0.5228728722491538 0.0 24 3.834433016768934E-4 0.0 0.0 0.5741584138484382 0.0 25 3.834433016768934E-4 0.0 0.0 0.633448334370228 0.0 26 3.834433016768934E-4 0.0 0.0 0.6948471930512405 0.0 27 3.834433016768934E-4 0.0 0.0 0.756006399668705 0.0 28 3.834433016768934E-4 0.0 0.0 0.816111137206558 0.0 29 3.834433016768934E-4 0.0 0.0 0.874250727823317 0.0 30 3.834433016768934E-4 0.0 0.0 0.9375188726000044 0.0 31 3.834433016768934E-4 0.0 0.0 1.008312092172101 0.0 32 4.313737143865051E-4 0.0 0.0 1.08246044063387 0.0 33 4.313737143865051E-4 0.0 0.0 1.1656197066850464 0.0 34 4.313737143865051E-4 0.0 0.0 1.2474369211803535 0.0 35 4.313737143865051E-4 0.0 0.0 1.3423870687580943 0.0 36 4.313737143865051E-4 0.0 0.0 1.4447664303058247 0.0 37 4.313737143865051E-4 4.7930412709611674E-5 0.0 1.5452765057578806 0.0 38 4.313737143865051E-4 4.7930412709611674E-5 0.0 1.6426231739711017 0.0 39 4.313737143865051E-4 4.7930412709611674E-5 0.0 1.7432770406612863 0.0 40 4.313737143865051E-4 4.7930412709611674E-5 0.0 1.8442664202404382 0.0 41 4.313737143865051E-4 4.7930412709611674E-5 0.0 1.9537394828691912 0.0 42 4.793041270961168E-4 4.7930412709611674E-5 0.0 2.072127602261932 0.0 43 4.793041270961168E-4 4.7930412709611674E-5 0.0 2.205230358356524 0.0 44 4.793041270961168E-4 4.7930412709611674E-5 0.0 2.3462416325482014 0.0 45 4.793041270961168E-4 9.586082541922335E-5 0.0 2.4871570459144596 0.0 46 5.272345398057285E-4 9.586082541922335E-5 0.0 2.6482032326187546 0.0 47 5.272345398057285E-4 9.586082541922335E-5 0.0 2.811022844593306 0.0 48 5.272345398057285E-4 1.4379123812883503E-4 0.0 2.972069031297601 0.0 49 5.751649525153401E-4 1.4379123812883503E-4 0.0 3.1412154577498206 0.0 50 5.751649525153401E-4 1.4379123812883503E-4 0.0 3.3083488068682363 0.0 51 5.751649525153401E-4 1.4379123812883503E-4 0.0 3.48933404525973 0.0 52 5.751649525153401E-4 1.4379123812883503E-4 0.0 3.6800012270185656 0.0 53 5.751649525153401E-4 1.4379123812883503E-4 0.0 3.880637934621 0.0 54 5.751649525153401E-4 1.4379123812883503E-4 0.0 4.1067736217849475 0.0 55 5.751649525153401E-4 1.4379123812883503E-4 0.0 4.33358033472683 0.0 56 7.668866033537868E-4 1.4379123812883503E-4 0.0 4.576347875101013 0.0 57 7.668866033537868E-4 1.4379123812883503E-4 0.0 4.82366880468261 4.7930412709611674E-5 58 8.148170160633986E-4 1.4379123812883503E-4 0.0 5.0761182884241345 4.7930412709611674E-5 59 8.148170160633986E-4 1.4379123812883503E-4 0.0 5.331539457753655 4.7930412709611674E-5 60 8.148170160633986E-4 1.4379123812883503E-4 0.0 5.5894530085440755 4.7930412709611674E-5 61 8.627474287730102E-4 1.4379123812883503E-4 0.0 5.864813229560794 4.7930412709611674E-5 62 8.627474287730102E-4 1.4379123812883503E-4 0.0 6.146644056293311 4.7930412709611674E-5 63 8.627474287730102E-4 1.4379123812883503E-4 0.0 6.442806076426002 4.7930412709611674E-5 64 8.627474287730102E-4 1.4379123812883503E-4 0.0 6.757373375039183 4.7930412709611674E-5 65 8.627474287730102E-4 1.4379123812883503E-4 0.0 7.091735934101434 4.7930412709611674E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCGAT 60 4.5896767E-4 29.581884 26 TTCGCGA 70 0.0011298283 25.355902 25 CGGTATA 190 1.8189894E-11 24.284208 10 ATTCGCG 220 0.0 24.205101 56 CGGTTAC 335 0.0 23.306704 4 CGATCGT 255 0.0 22.268614 1 CGCGGAA 230 1.0913936E-11 21.606602 59 GCGTAAG 345 0.0 20.574757 38 TGCGTAA 350 0.0 20.281805 37 CGCTCCG 300 1.8189894E-12 18.932407 25 CGTAAGT 360 0.0 18.73295 39 AACGGGC 95 0.0066540306 18.68016 30 CGCGTCA 95 0.006656793 18.678816 2 AATTCGC 305 1.8189894E-12 18.623379 55 TTGCGTA 365 0.0 18.47589 36 GCGGTAT 275 1.3096724E-10 18.068377 9 ATTTGCG 315 1.8189894E-12 18.02611 3 TCGCGCT 320 3.6379788E-12 17.746578 37 TTGCGCG 340 0.0 17.744877 5 CAATTCG 355 1.8189894E-12 17.001205 54 >>END_MODULE