FastQCFastQC Report
Thu 26 May 2016
SRR936111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936111_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086358
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129520.6207947054148905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94680.4538051475346034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66440.31844966204266No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21210.10166040535708636No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20970.10051007545205567No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA14200.049.88713100-101
CGTACAT20500.043.41275100-101
ACGTATC4050.042.62583100-101
CGGTATC12650.042.35278100-101
CCGTACA7400.041.831318100-101
GGTATCA108150.041.481661
GCGTACA6100.039.035534100-101
AGGTATC23000.038.823383100-101
TACGCAG10000.038.693974100-101
AGTATCA34250.038.063915100-101
CGAGTAC12100.037.636215100-101
TCGAGTA4750.036.970757100-101
CCGTATC6300.034.961586100-101
CCGGTAT4950.034.875683100-101
CCCGTAC3600.034.7245298-99
GGTACAT21050.033.79448100-101
CCTGTCG6450.033.67978796-97
ACGTACA7550.033.509804100-101
TCGTATC5200.033.198967100-101
GTATCAA212750.031.9380071
Produced by FastQC (version 0.11.5)