FastQCFastQC Report
Thu 26 May 2016
SRR936110_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936110_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086894
Sequences flagged as poor quality0
Sequence length77
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143620.6881997839851952No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48800.23384033880014987No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA1700.029.23274610
GCGGTAT2257.2759576E-1222.0853779
CGCGGAA2501.8189894E-1221.3022359
CGATCGT2653.6379788E-1220.0887371
ATTCGCG2850.019.9356
TACGTTA900.004842874619.73611671
TCGGCGA900.00486846319.71814213
CGCTCCG2755.456968E-1219.3605625
TCGCGGG1851.9533036E-719.1944263
GACGTAT3550.018.998839
GCGTAAG3950.018.87177538
GCGCGAG2455.493348E-1018.83554624
ACGGATA950.00665641418.67930
TGCGTAA4000.018.63498337
TTGCGTA4050.018.403636
ACGATCG1953.4029472E-718.20703559
CGGTTAC2751.3096724E-1018.0681234
CGCGCTT3151.8189894E-1218.03017638
TCGCGCT3002.3646862E-1117.74760437
CGGAGCG2004.4573608E-717.74462735
Produced by FastQC (version 0.11.5)