##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936110_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2086894 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.60608444894662 39.0 36.0 41.0 30.0 41.0 2 36.46272834173657 39.0 36.0 41.0 30.0 41.0 3 36.32264216582155 39.0 36.0 41.0 29.0 41.0 4 36.215586416943076 39.0 35.0 41.0 29.0 41.0 5 36.13407245408727 39.0 35.0 41.0 29.0 41.0 6 36.00873355330937 39.0 35.0 40.0 29.0 41.0 7 35.827236553461745 39.0 35.0 40.0 28.0 41.0 8 35.659163330768116 38.0 35.0 40.0 28.0 41.0 9 35.77860351316358 39.0 35.0 40.0 29.0 41.0 10-11 35.95704046300387 39.0 35.0 41.0 29.0 41.0 12-13 35.82109177562444 39.0 35.0 41.0 28.0 41.0 14-15 35.6308015644302 39.0 35.0 41.0 27.0 41.0 16-17 35.43363270966326 39.0 35.0 41.0 26.5 41.0 18-19 35.20431392298794 39.0 35.0 41.0 25.5 41.0 20-21 34.881724467078826 39.0 34.5 41.0 24.0 41.0 22-23 34.46833140542836 38.5 34.0 41.0 22.5 41.0 24-25 34.13106894744055 38.0 33.5 40.0 19.5 41.0 26-27 33.147616266087304 37.5 32.0 39.5 16.5 40.5 28-29 33.13450275864514 37.5 32.5 39.5 15.0 40.5 30-31 33.51657702787013 38.0 33.0 40.0 14.5 41.0 32-33 33.37900583354977 38.0 33.0 40.0 12.5 41.0 34-35 33.11781767545453 37.5 33.0 40.0 8.5 41.0 36-37 32.807957902988846 37.0 32.0 40.0 8.0 41.0 38-39 32.49868273137016 36.5 32.0 40.0 6.5 41.0 40-41 32.12882398435187 36.0 31.5 39.5 2.0 41.0 42-43 31.747431589721373 35.5 31.0 39.0 2.0 41.0 44-45 31.37814833911066 35.0 31.0 39.0 2.0 40.5 46-47 30.959669489681794 35.0 31.0 38.0 2.0 40.0 48-49 30.542462626276176 35.0 30.0 37.0 2.0 39.5 50-51 30.09568526240432 35.0 30.0 37.0 2.0 39.0 52-53 29.6124582753125 34.5 29.0 36.0 2.0 39.0 54-55 29.152798608841657 34.0 29.0 36.0 2.0 38.5 56-57 28.71399385881602 34.0 29.0 35.0 2.0 37.0 58-59 28.2843762069372 34.0 28.0 35.0 2.0 37.0 60-61 27.92434594186384 34.0 27.0 35.0 2.0 36.0 62-63 27.549864535525046 34.0 26.5 35.0 2.0 36.0 64-65 27.156653140983682 34.0 26.0 35.0 2.0 35.5 66-67 26.788996709943103 33.0 25.0 35.0 2.0 35.0 68-69 26.40714238480728 33.0 25.0 35.0 2.0 35.0 70-71 25.951069867468114 33.0 23.5 35.0 2.0 35.0 72-73 25.469343675337605 33.0 20.5 35.0 2.0 35.0 74-75 24.90036412965872 32.5 10.0 35.0 2.0 35.0 76-77 23.197207668429733 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9174916223352199 1101 2 0.9269274987919331 1101 3 0.9625844748476098 1101 4 0.7419067234041066 1101 5 0.9310018506671085 1101 6 0.8307788952685442 1101 7 0.8661530271293927 1101 8 0.9670449630015483 1101 9 0.9294773472840632 1101 10-11 0.9946654984896455 1101 12-13 1.1197426802777812 1101 14-15 1.236619645449828 1101 16-17 1.2085951997654902 1101 18-19 1.2677767852085466 1101 20-21 1.2095837072920688 1101 22-23 1.1111400817380144 1101 24-25 1.2447550861055845 1101 26-27 1.0933221967565956 1101 28-29 0.9663429512255917 1101 30-31 1.1126257636849388 1101 32-33 1.1351036535515178 1101 34-35 1.1439507551747141 1101 36-37 1.0945392861936938 1101 38-39 1.14338299371245 1101 40-41 1.1050287533420757 1101 42-43 1.0712761734088652 1101 44-45 1.0581226411255464 1101 46-47 1.065312887902138 1101 48-49 1.1490428932473904 1101 50-51 1.0415044084269418 1101 52-53 1.079611322521103 1101 54-55 0.9706886771490879 1101 56-57 1.0684238261501164 1101 58-59 1.0688821243727595 1101 60-61 0.9742170109308965 1101 62-63 0.9755642583341348 1101 64-65 0.9622264442943624 1101 66-67 1.1803835938161278 1101 68-69 1.1059226395593527 1101 70-71 1.0281179948178512 1101 72-73 0.929981788145966 1101 74-75 0.8829532472781061 1101 76-77 0.9799494859424698 1102 1 0.9625825683225813 1102 2 0.97219549127108 1102 3 0.9353555526983683 1102 4 1.1069900491448053 1102 5 0.997544412229594 1102 6 0.8061363014028444 1102 7 0.9520081513311283 1102 8 0.9694645983893153 1102 9 0.9775905079470277 1102 10-11 1.1329853997577501 1102 12-13 1.263789673179346 1102 14-15 1.2416706472637742 1102 16-17 1.2469987580893687 1102 18-19 1.3090560548244738 1102 20-21 1.4230562205653783 1102 22-23 1.3174163281039029 1102 24-25 1.460777690076398 1102 26-27 1.348644980465835 1102 28-29 1.4182732756658822 1102 30-31 1.454534635474289 1102 32-33 1.3862435844723322 1102 34-35 1.2468137315436678 1102 36-37 1.1619851990081287 1102 38-39 1.355330422293342 1102 40-41 1.422601497707653 1102 42-43 1.3587582616877576 1102 44-45 1.2954907363123702 1102 46-47 1.4658075827195347 1102 48-49 1.3072059789503179 1102 50-51 1.3266590083225793 1102 52-53 1.3958039325384135 1102 54-55 1.4821594261696411 1102 56-57 1.5598158464265985 1102 58-59 1.564918767819762 1102 60-61 1.3091496297280543 1102 62-63 1.2500541366586724 1102 64-65 1.3140292124317945 1102 66-67 1.3252474854429686 1102 68-69 1.3105412752038852 1102 70-71 1.2944720173969735 1102 72-73 1.3414999566362447 1102 74-75 1.3692595811939974 1102 76-77 1.3666185063266312 1103 1 0.5882623328585836 1103 2 0.38991632927192654 1103 3 0.3875711728025095 1103 4 0.6086706060690474 1103 5 0.4046575045347822 1103 6 0.534190515641292 1103 7 0.4598925652152204 1103 8 0.46355311056976944 1103 9 0.5653505229098883 1103 10-11 0.578554964004546 1103 12-13 0.6778875723449289 1103 14-15 0.5051836070349864 1103 16-17 0.6329961617106292 1103 18-19 0.6608031552667271 1103 20-21 0.551824338401957 1103 22-23 0.4892473030431148 1103 24-25 0.763742509244949 1103 26-27 0.7659726410501477 1103 28-29 0.6851816643222648 1103 30-31 0.837196818644685 1103 32-33 0.7766896246427919 1103 34-35 0.8494373109262909 1103 36-37 0.9047396984259066 1103 38-39 0.7806282215053812 1103 40-41 0.7764976244575976 1103 42-43 0.7624137055357956 1103 44-45 0.7172696730640986 1103 46-47 0.6642829895224125 1103 48-49 0.6836166663370626 1103 50-51 0.6070686670124026 1103 52-53 0.6601420872391444 1103 54-55 0.6838139388609257 1103 56-57 0.8318385582044243 1103 58-59 0.8393586156973107 1103 60-61 0.5899476893824236 1103 62-63 0.6117209223176516 1103 64-65 0.5707300717909263 1103 66-67 0.5474934083641081 1103 68-69 0.41672544815368084 1103 70-71 0.2878522320255854 1103 72-73 0.4647899868234191 1103 74-75 0.40854564742880584 1103 76-77 0.4111746331570174 1104 1 0.4594535004357141 1104 2 0.3179589720016196 1104 3 0.41036344193351226 1104 4 0.6018571155327166 1104 5 0.5054671766175858 1104 6 0.37972807293058963 1104 7 0.42634940019705425 1104 8 0.3828772842794166 1104 9 0.32685286446156425 1104 10-11 0.4760990836360932 1104 12-13 0.5707307208697756 1104 14-15 0.7397412361951794 1104 16-17 0.6061492846958174 1104 18-19 0.3961438422884811 1104 20-21 0.19531372520030743 1104 22-23 0.3930440153746275 1104 24-25 0.5222082056379165 1104 26-27 0.2316535402859543 1104 28-29 0.24599703409749907 1104 30-31 0.44536438577999604 1104 32-33 0.5394397108578701 1104 34-35 0.636022244028382 1104 36-37 0.5876404986018358 1104 38-39 0.5604426466631907 1104 40-41 0.5455512827259952 1104 42-43 0.5652727976035017 1104 44-45 0.45208169919351704 1104 46-47 0.37786923709214904 1104 48-49 0.4135218047560052 1104 50-51 0.4813363386054057 1104 52-53 0.3335588534488316 1104 54-55 0.24998613911981593 1104 56-57 0.28322024861980566 1104 58-59 0.17728613005399296 1104 60-61 0.07611641604812291 1104 62-63 0.033973410060117004 1104 64-65 -0.0665317121865634 1104 66-67 -0.02618687612067916 1104 68-69 -0.07561952713132669 1104 70-71 0.01847945300108833 1104 72-73 0.012239769661370303 1104 74-75 -0.30473947209778274 1104 76-77 -0.17744435115433532 1105 1 -0.0846410068145218 1105 2 0.11393805646263644 1105 3 0.09636165055632517 1105 4 -0.05460878778133349 1105 5 -0.11597851318366992 1105 6 0.08442680686266613 1105 7 0.1362080266464929 1105 8 -0.028764246621840073 1105 9 -0.11349064421693811 1105 10-11 0.05188583712807571 1105 12-13 -0.029882946853959425 1105 14-15 0.07462891200432153 1105 16-17 -0.044325188179342945 1105 18-19 -0.03803272940292146 1105 20-21 0.07760087812097538 1105 22-23 -0.0781862250088281 1105 24-25 0.009262479524963396 1105 26-27 -0.33130368453187486 1105 28-29 -0.1509576246935609 1105 30-31 0.012933801645793608 1105 32-33 0.021487206560991012 1105 34-35 0.11830697616908026 1105 36-37 0.0773673135816253 1105 38-39 0.09278305674331477 1105 40-41 0.3265039806852812 1105 42-43 0.3215087268199035 1105 44-45 0.014423440702632462 1105 46-47 0.017457462418349223 1105 48-49 0.11056966357611486 1105 50-51 0.07378955703238432 1105 52-53 -0.00530434827476256 1105 54-55 -0.02646451328038424 1105 56-57 0.028040989862635257 1105 58-59 0.05419491506452445 1105 60-61 0.029956378283955587 1105 62-63 -0.18309066798583018 1105 64-65 -0.12421950419314598 1105 66-67 -0.15218544786049293 1105 68-69 -0.1581082099849418 1105 70-71 -0.336484961533273 1105 72-73 -0.5556437424062182 1105 74-75 -0.8362289780165888 1105 76-77 -0.7879559830021208 1106 1 -0.06883927004663093 1106 2 -0.2861503512285921 1106 3 -0.29324864881990464 1106 4 -0.27224240857942306 1106 5 0.007103920399757158 1106 6 -0.19163327682515785 1106 7 -0.4189053041072839 1106 8 -0.17151655294849633 1106 9 -0.09938039335711579 1106 10-11 -0.3743044023273896 1106 12-13 -0.06353393293514387 1106 14-15 -0.06208627507137976 1106 16-17 -0.04007562931934672 1106 18-19 -0.12044043740357324 1106 20-21 -0.0017892286031582216 1106 22-23 0.01498458032773442 1106 24-25 -0.14891289593818868 1106 26-27 -0.16795837582970563 1106 28-29 -0.05489895457045435 1106 30-31 0.15660923760471945 1106 32-33 -0.08374442039209384 1106 34-35 -0.08400288440312664 1106 36-37 -0.045258706311301466 1106 38-39 0.059714746827637555 1106 40-41 -0.0902054323379673 1106 42-43 0.11399503141434408 1106 44-45 -0.07976021968724822 1106 46-47 -0.1334072678243885 1106 48-49 -0.12250523498863686 1106 50-51 -0.20164448519965816 1106 52-53 -0.1164554571444043 1106 54-55 0.022594208353982737 1106 56-57 -0.008894857060283812 1106 58-59 -0.07566148832371766 1106 60-61 -0.2963743790872435 1106 62-63 -0.26584661603852666 1106 64-65 -0.09123337270048282 1106 66-67 -0.1525662717770473 1106 68-69 0.049946832787366446 1106 70-71 -0.044400444738808886 1106 72-73 -0.09372313922138176 1106 74-75 0.0340980226267007 1106 76-77 0.08304713282992537 1107 1 0.02142553855950524 1107 2 0.0409120004141883 1107 3 0.16586022304429804 1107 4 0.10662614777853463 1107 5 0.12205785820610515 1107 6 -0.20972478706551811 1107 7 -0.0018579845088382285 1107 8 0.07389665454418548 1107 9 -0.10840666292865109 1107 10-11 0.06121498262417191 1107 12-13 8.585276130759212E-4 1107 14-15 -0.20717520077673868 1107 16-17 -0.023515495390242336 1107 18-19 0.08081652134364248 1107 20-21 -0.02739687839479643 1107 22-23 -0.050848871757878555 1107 24-25 -0.1890952665876 1107 26-27 -0.34882722940097466 1107 28-29 -0.2077785782505046 1107 30-31 -0.0831545693737823 1107 32-33 0.12015341711110494 1107 34-35 -0.08408810433275704 1107 36-37 -0.025681599060732196 1107 38-39 0.0058977743513253245 1107 40-41 -0.03571879085539109 1107 42-43 0.06114564554953361 1107 44-45 -0.030424219103149852 1107 46-47 -0.11905111378338162 1107 48-49 0.030388357247382203 1107 50-51 0.012548178045221903 1107 52-53 0.10742868413098705 1107 54-55 0.16682588730163062 1107 56-57 0.11539301069716501 1107 58-59 0.05426087232936894 1107 60-61 -0.0033779313262414234 1107 62-63 0.06475377127783943 1107 64-65 0.07778850205814791 1107 66-67 0.2538573154091601 1107 68-69 0.11047176407733517 1107 70-71 0.18998018596849064 1107 72-73 0.05830475469365837 1107 74-75 -0.004673054339573213 1107 76-77 -0.09559003850697323 1108 1 0.23920835977433796 1108 2 0.4287836805347993 1108 3 0.38628225194482013 1108 4 0.5564495294483294 1108 5 0.46765923151712485 1108 6 0.46674739809257204 1108 7 0.4410947699181591 1108 8 0.2737728610892205 1108 9 0.3095111727853137 1108 10-11 0.2791476816970331 1108 12-13 0.3973787822764834 1108 14-15 0.3375663374021798 1108 16-17 0.4337018983141192 1108 18-19 0.4774502307176931 1108 20-21 0.4527862146588859 1108 22-23 0.4388938269846818 1108 24-25 0.45845083131690956 1108 26-27 0.3085325313012106 1108 28-29 0.43803323488472046 1108 30-31 0.5599552570484221 1108 32-33 0.4466850104586584 1108 34-35 0.46660171840641596 1108 36-37 0.6022042566773607 1108 38-39 0.558900466949467 1108 40-41 0.446226074001153 1108 42-43 0.5493283702995804 1108 44-45 0.4125237844785339 1108 46-47 0.5086978723636051 1108 48-49 0.6025465712553135 1108 50-51 0.5427159326554616 1108 52-53 0.6678822857920679 1108 54-55 0.7452651712723259 1108 56-57 0.6777910654748638 1108 58-59 0.6811065191750565 1108 60-61 0.7220005866181545 1108 62-63 0.6185567550801707 1108 64-65 0.7008864106455484 1108 66-67 0.6581592284614537 1108 68-69 0.5728590955184707 1108 70-71 0.5887926103791514 1108 72-73 0.5225035155827058 1108 74-75 0.5921668190979084 1108 76-77 0.5962074687018699 1109 1 0.03690818942620666 1109 2 0.06196692286790295 1109 3 -0.07259106614188227 1109 4 0.019168341197286054 1109 5 0.03196912439185695 1109 6 -0.004657779038559795 1109 7 0.13895495358580234 1109 8 0.1805573417963231 1109 9 0.2190272290266364 1109 10-11 0.06956088235512681 1109 12-13 0.15548286718075133 1109 14-15 0.31646721325420657 1109 16-17 0.2969482245803121 1109 18-19 0.2260911654498443 1109 20-21 0.2847891653878918 1109 22-23 0.467492459269792 1109 24-25 0.3154832153885323 1109 26-27 0.12526465270237708 1109 28-29 0.4134163389135992 1109 30-31 0.3609431731319219 1109 32-33 0.44711849961952765 1109 34-35 0.4178672961178336 1109 36-37 0.5841710403603315 1109 38-39 0.6146779396751612 1109 40-41 0.664928582344892 1109 42-43 0.6199422868590325 1109 44-45 0.4468141328070736 1109 46-47 0.4636753900852355 1109 48-49 0.5337737231609445 1109 50-51 0.46486390113859244 1109 52-53 0.5388678893358083 1109 54-55 0.7408600295463543 1109 56-57 0.6885696967112693 1109 58-59 0.7574802731037984 1109 60-61 0.7151185400384072 1109 62-63 0.43925190183905016 1109 64-65 0.5565907649875861 1109 66-67 0.7141161919174976 1109 68-69 0.533550401489439 1109 70-71 0.5227985073555033 1109 72-73 0.31854266383308527 1109 74-75 0.30889212836648383 1109 76-77 0.40372931794489375 1110 1 -0.18600765858341362 1110 2 -0.43405227751042474 1110 3 -0.49073578089199543 1110 4 -0.18984947448112166 1110 5 -0.4107257329959566 1110 6 -0.3726717638272774 1110 7 -0.3703511569554365 1110 8 -0.640564817995326 1110 9 -0.6031206974107519 1110 10-11 -0.5481645524049199 1110 12-13 -0.413230739792418 1110 14-15 -0.42742358512566625 1110 16-17 -0.46154252480771163 1110 18-19 -0.5692488260106074 1110 20-21 -0.5717512877526048 1110 22-23 -0.6226219060562244 1110 24-25 -0.7342611509751649 1110 26-27 -0.57833705554782 1110 28-29 -0.41623814103291323 1110 30-31 -0.28491259353084075 1110 32-33 -0.2988600868638329 1110 34-35 -0.3508913263609088 1110 36-37 -0.31731177081865525 1110 38-39 -0.31816198799382533 1110 40-41 -0.2784768055667044 1110 42-43 -0.5018414541442517 1110 44-45 -0.4111147414182845 1110 46-47 -0.5900594509379467 1110 48-49 -0.6081246340814275 1110 50-51 -0.7253721144979686 1110 52-53 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-0.634991525346642 2314 20-21 -0.5541709363806078 2314 22-23 -0.43580276274580854 2314 24-25 -0.3321620264672589 2314 26-27 -0.4081654825999337 2314 28-29 -0.5409186451753456 2314 30-31 -0.652785265250678 2314 32-33 -0.663173501283012 2314 34-35 -0.7977587980102498 2314 36-37 -1.4311896426090058 2314 38-39 -1.0304232841921923 2314 40-41 -0.8370672045325662 2314 42-43 -0.5942183564299306 2314 44-45 -0.5765195830658563 2314 46-47 -0.31180573450337334 2314 48-49 -0.2377584257260601 2314 50-51 -0.14070350882195726 2314 52-53 -0.2035658016396269 2314 54-55 -0.47671001410206415 2314 56-57 -0.3805660685364991 2314 58-59 -0.5298129610026514 2314 60-61 -0.6995287714214449 2314 62-63 -0.7304337478687906 2314 64-65 -0.5158439179973087 2314 66-67 -0.6056159460768562 2314 68-69 -0.5921691943931364 2314 70-71 -0.47445391820488325 2314 72-73 -0.483213484680455 2314 74-75 -0.4269335152725944 2314 76-77 -0.5215991149913748 2315 1 -1.1669577121141117 2315 2 -1.0401768876672932 2315 3 -1.0357539347811198 2315 4 -0.978461400189822 2315 5 -1.1315049537751776 2315 6 -1.2222330448401735 2315 7 -1.5102815670471372 2315 8 -1.6135725450353107 2315 9 -1.2560613947062933 2315 10-11 -1.3609583893923158 2315 12-13 -1.1418300974240339 2315 14-15 -1.185927402097228 2315 16-17 -1.0100023535094778 2315 18-19 -0.9200610961777116 2315 20-21 -0.9437605508909002 2315 22-23 -1.1886043107483104 2315 24-25 -1.12889295286503 2315 26-27 -1.0584317816102882 2315 28-29 -1.0149150018065498 2315 30-31 -1.0644243004619014 2315 32-33 -1.1080696944605393 2315 34-35 -1.1861826031844487 2315 36-37 -1.19602400840186 2315 38-39 -1.0651130655900651 2315 40-41 -1.1216173120136652 2315 42-43 -0.9487966047284324 2315 44-45 -0.9868054038024958 2315 46-47 -1.0235039186566084 2315 48-49 -1.011507541496595 2315 50-51 -0.9382526479430169 2315 52-53 -0.8108218058152517 2315 54-55 -0.9731619360563606 2315 56-57 -1.1027682119774056 2315 58-59 -0.9045790265077436 2315 60-61 -1.0678601359849615 2315 62-63 -0.997033653779912 2315 64-65 -0.9716268645589459 2315 66-67 -1.1332470867500355 2315 68-69 -1.3602444758013128 2315 70-71 -1.3503536860731877 2315 72-73 -1.229579988351297 2315 74-75 -1.2737440223223935 2315 76-77 -1.3375304404084218 2316 1 -2.2954551338100657 2316 2 -1.858139419087486 2316 3 -2.0994683868376 2316 4 -2.8838920108505093 2316 5 -2.3213637785367567 2316 6 -2.28707357966114 2316 7 -1.8975458385915331 2316 8 -1.7914220022668843 2316 9 -2.093117468968842 2316 10-11 -1.9831658042497509 2316 12-13 -2.682307372461608 2316 14-15 -3.895536956146117 2316 16-17 -2.962662596492301 2316 18-19 -2.4660622220679542 2316 20-21 -2.4351964305946865 2316 22-23 -2.790464647991712 2316 24-25 -2.651237484519335 2316 26-27 -2.5314184056954048 2316 28-29 -3.12146574618788 2316 30-31 -3.2795346022805774 2316 32-33 -3.266611905333157 2316 34-35 -3.1510186521568215 2316 36-37 -3.1027060360516288 2316 38-39 -3.217475779370588 2316 40-41 -3.071874641851757 2316 42-43 -3.2528083395543277 2316 44-45 -3.003731492419764 2316 46-47 -2.962453637107039 2316 48-49 -2.818202314347083 2316 50-51 -3.0305216440678606 2316 52-53 -3.05906317989421 2316 54-55 -3.2261864183932865 2316 56-57 -2.926913705952124 2316 58-59 -3.143836285496384 2316 60-61 -3.030776511296086 2316 62-63 -2.9102229535100648 2316 64-65 -2.8218912600898847 2316 66-67 -2.8181641620845674 2316 68-69 -3.022467681615897 2316 70-71 -2.721874361792157 2316 72-73 -2.9987932821732244 2316 74-75 -3.155415615079729 2316 76-77 -3.3728564980675593 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49998.0 3 5460.0 4 7847.0 5 9729.0 6 10642.0 7 11028.0 8 13726.0 9 15198.0 10 20239.0 11 23009.0 12 16097.0 13 11674.0 14 11384.0 15 11460.0 16 11265.0 17 11976.0 18 12462.0 19 13049.0 20 13908.0 21 15060.0 22 16476.0 23 18938.0 24 21707.0 25 25450.0 26 29129.0 27 34544.0 28 40383.0 29 47191.0 30 55615.0 31 66923.0 32 81250.0 33 101278.0 34 135686.0 35 178952.0 36 246918.0 37 327878.0 38 289107.0 39 73771.0 40 487.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.72178144139404 26.398546693137476 30.529698916105648 21.349972949362837 2 21.558082831435 26.731980540018323 30.446858838458386 21.263077790088293 3 21.722694955249573 26.805496184376743 30.00276994504199 21.46903891533169 4 21.391911056027553 26.86962593822174 30.326470733424486 21.411992272326213 5 21.683661366096832 26.79807785851931 30.242385932155706 21.275874843228152 6 21.742539296560537 26.694871594573648 30.33499422275024 21.227594886115575 7 21.576210052708714 26.524301698200198 30.347720316961585 21.551767932129504 8 21.445006726456324 26.70812930707675 30.126424789016443 21.720439177450483 9 21.541196994095547 26.720151828847484 30.07255961966107 21.666091557395905 10-11 21.871944626568666 26.75733097913815 29.925405753591683 21.445318640701505 12-13 21.95388355912602 26.490929072164356 29.805018066130813 21.750169302578815 14-15 21.660829827875553 26.807335439962504 29.946505228714948 21.585329503446992 16-17 21.854404602682695 26.7125522874204 29.907134570293124 21.525908539603783 18-19 21.81389527802465 26.750352297704676 29.523350370939948 21.91240205333072 20-21 21.862892696266965 26.873484883519488 29.44381252607312 21.819809894140427 22-23 22.068987827087128 27.12110610562638 28.780024920923992 22.029881146362502 24-25 22.13148578971131 27.29648886192118 28.42970774225645 22.14231760611106 26-27 22.164516272031612 27.34393813956219 28.2605825414873 22.2309630469189 28-29 22.257454988769656 27.518680900555264 28.142567630059467 22.081296480615613 30-31 22.06547931820311 27.348022679280536 28.225918719080216 22.360579283436135 32-33 22.099944075099426 27.698999437875678 27.982577500554694 22.2184789864702 34-35 22.107718820027106 27.308150891458826 28.24966502004129 22.334465268472776 36-37 22.027244488276896 27.485846303362095 28.434481826624765 22.052427381736244 38-39 22.04459499386436 27.34949710758684 28.445812872215033 22.160095026333767 40-41 21.90106725069129 27.303574594928765 28.499738818308597 22.295619336071347 42-43 21.85556130917707 27.56036889526713 28.131463089506248 22.452606706049554 44-45 21.948734956316613 27.22484044823305 28.421769251948852 22.40465534350148 46-47 21.771851971132662 27.446593815380627 28.430620544292708 22.35093366919401 48-49 21.931544988223894 27.325190898959356 28.25182392462909 22.491440188187664 50-51 21.651519102131747 27.596502815424344 28.104108391440803 22.647869691003102 52-53 21.62991065437046 27.502444542439175 28.181284391356865 22.686360411833505 54-55 21.568040091275133 27.4707527993869 28.215850067986654 22.745357041351316 56-57 21.571343642372064 27.634990730204006 28.087517800955663 22.706147826468268 58-59 21.60933333876486 27.706690047779038 27.913894692498676 22.77008192095743 60-61 21.5276799279186 27.330160601572945 28.00932648943461 23.13283298107384 62-63 21.551313638639197 27.272197870062758 27.96784604290789 23.208642448390158 64-65 21.53560399412513 27.117352886835917 28.09479636973527 23.252246749303684 66-67 21.30972843972238 27.29019875951137 28.16364021084904 23.236432589917214 68-69 21.49734447867015 26.93971927037811 28.219105134487222 23.34383111646452 70-71 21.252123009285505 26.823882221377882 28.383684261624264 23.540310507712352 72-73 21.386632871964686 26.628120184087145 28.264128036272723 23.721118907675447 74-75 21.224779774474293 26.5279355950483 28.21917460055613 24.028110029921276 76-77 21.058822598554162 26.461405889189106 28.540827534384295 23.938943977872444 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14682.0 1 8755.0 2 2699.0 3 2605.5 4 2641.0 5 2746.5 6 2937.5 7 3126.0 8 3291.5 9 3354.0 10 3463.5 11 3589.5 12 3860.0 13 4114.0 14 4285.5 15 4666.0 16 5225.0 17 5575.0 18 5995.5 19 6807.0 20 7846.0 21 9591.5 22 10689.0 23 11820.5 24 14211.0 25 17432.0 26 19394.0 27 21885.5 28 27742.0 29 33417.0 30 35727.0 31 40180.0 32 49139.5 33 57235.5 34 62395.5 35 63966.0 36 67967.5 37 74359.0 38 77523.5 39 78298.0 40 79808.0 41 82678.5 42 85835.0 43 87631.0 44 87594.5 45 88447.0 46 89528.0 47 89794.0 48 89868.0 49 90751.5 50 91467.5 51 89182.0 52 87064.0 53 84953.0 54 78993.5 55 71283.0 56 63218.0 57 59015.0 58 56766.0 59 49281.5 60 41172.5 61 38299.0 62 33924.5 63 24661.0 64 17018.0 65 14264.0 66 12535.5 67 9651.0 68 7218.0 69 5078.0 70 4215.0 71 3511.0 72 2326.5 73 1680.0 74 1514.0 75 1415.0 76 1017.5 77 598.5 78 478.0 79 379.0 80 235.0 81 148.5 82 95.5 83 84.0 84 75.0 85 59.0 86 52.5 87 53.0 88 50.0 89 39.5 90 31.0 91 30.0 92 27.0 93 24.5 94 23.5 95 19.0 96 16.0 97 17.5 98 20.0 99 40.0 100 59.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003210512848280747 2 0.00718771533197182 3 0.01006280146476055 4 0.017585943512224388 5 0.01260246088205726 6 0.012506624677630968 7 0.015908809934764297 8 0.017106762490092933 9 0.016004646139190587 10-11 0.01945474949853706 12-13 0.01883181416976617 14-15 0.0070918791275455285 16-17 0.005318909345659147 18-19 0.011284713071195758 20-21 0.010278432924719704 22-23 0.014087922050664767 24-25 0.02146730979148917 26-27 0.01253058372873754 28-29 0.00656478000320093 30-31 0.0068762476675863745 32-33 0.008002323069595293 34-35 0.009679456647055384 36-37 0.007666896354103275 38-39 0.015070243146034251 40-41 0.010733654895744585 42-43 0.013680618181853031 44-45 0.01655570431464176 46-47 0.015070243146034251 48-49 0.012147238911032376 50-51 0.0253965941729671 52-53 0.029421714758871315 54-55 0.02180273650698119 56-57 0.013345191466361013 58-59 0.014830652634968524 60-61 0.017442189205584952 62-63 0.023695501544400435 64-65 0.018184919789888704 66-67 0.018089083585462414 68-69 0.027816458334730943 70-71 0.05350056112097692 72-73 0.06516861900987783 74-75 0.07611790536558158 76-77 0.07333865543721914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2086894.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.131006645651006 #Duplication Level Percentage of deduplicated Percentage of total 1 76.3677116776145 41.33861108334109 2 12.20576677105124 13.214208843980815 3 4.611225526840408 7.48830838913981 4 2.225527089361435 4.8188008665720075 5 1.2584924216589395 3.406173082016074 6 0.7909442417219618 2.5688764802994544 7 0.48336078975584235 1.8315364285764433 8 0.3466395983872095 1.5011160323155064 9 0.25552364615684164 1.2448576969413272 >10 1.3043377140013754 12.734923986338073 >50 0.10073062675912214 3.730149526665412 >100 0.04769436424736506 4.5152150224265535 >500 0.0017787238640859777 0.6256060262162346 >1k 1.7787238640859645E-4 0.29020031750915276 >5k 0.0 0.0 >10k+ 8.893619320429823E-5 0.691416217661986 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14362 0.6881997839851952 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4880 0.23384033880014987 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.9167240885258188E-4 0.0 2 0.0 0.0 0.0 7.18771533197182E-4 0.0 3 0.0 0.0 0.0 0.0011021163509023458 0.0 4 1.437543066394364E-4 0.0 0.0 0.0024917413150835643 0.0 5 1.9167240885258188E-4 0.0 0.0 0.004839728323527692 0.0 6 1.9167240885258188E-4 0.0 0.0 0.008816930807218766 0.0 7 1.9167240885258188E-4 0.0 0.0 0.017490107307798097 0.0 8 1.9167240885258188E-4 0.0 0.0 0.0285591889190347 0.0 9 1.9167240885258188E-4 0.0 0.0 0.03986786104133703 0.0 10 1.9167240885258188E-4 0.0 0.0 0.052374485718967995 0.0 11 1.9167240885258188E-4 0.0 0.0 0.06794786893824027 0.0 12 1.9167240885258188E-4 0.0 0.0 0.08601299347259611 0.0 13 1.9167240885258188E-4 0.0 0.0 0.11270337640531815 0.0 14 2.3959051106572734E-4 0.0 0.0 0.14361055233279696 0.0 15 2.3959051106572734E-4 0.0 0.0 0.18065124534355842 0.0 16 2.3959051106572734E-4 0.0 0.0 0.21563145995915461 0.0 17 2.3959051106572734E-4 0.0 0.0 0.24874286858843814 0.0 18 2.875086132788728E-4 0.0 0.0 0.28563980729256017 0.0 19 2.875086132788728E-4 0.0 0.0 0.32186589256569814 0.0 20 3.354267154920183E-4 0.0 0.0 0.36135040878933 0.0 21 3.354267154920183E-4 0.0 0.0 0.40591424384755526 0.0 22 3.8334481770516376E-4 0.0 0.0 0.4556532339448003 0.0 23 4.3126291991830924E-4 0.0 0.0 0.5065901765973739 0.0 24 4.3126291991830924E-4 0.0 0.0 0.5587729899074894 0.0 25 4.3126291991830924E-4 0.0 0.0 0.61469341519023 0.0 26 4.3126291991830924E-4 0.0 0.0 0.6712367758017417 0.0 27 4.3126291991830924E-4 0.0 0.0 0.7307510587504684 0.0 28 4.3126291991830924E-4 0.0 0.0 0.789594488268211 0.0 29 4.3126291991830924E-4 0.0 0.0 0.8483899996837405 0.0 30 4.3126291991830924E-4 0.0 0.0 0.9106356144586165 0.0 31 4.3126291991830924E-4 0.0 0.0 0.9798293540543985 0.0 32 5.750172265577456E-4 0.0 0.0 1.0553003650401027 0.0 33 5.750172265577456E-4 4.791810221314547E-5 0.0 1.1333110354431035 0.0 34 5.750172265577456E-4 4.791810221314547E-5 0.0 1.2216720159241437 0.0 35 5.750172265577456E-4 4.791810221314547E-5 0.0 1.3157352505685482 0.0 36 5.750172265577456E-4 4.791810221314547E-5 0.0 1.4129131618568074 0.0 37 5.750172265577456E-4 4.791810221314547E-5 0.0 1.5137807670154786 0.0 38 5.750172265577456E-4 4.791810221314547E-5 0.0 1.612779566187837 0.0 39 5.750172265577456E-4 4.791810221314547E-5 0.0 1.7104845766004406 0.0 40 5.750172265577456E-4 4.791810221314547E-5 0.0 1.8151856299361635 0.0 41 5.750172265577456E-4 4.791810221314547E-5 0.0 1.9235284590400854 0.0 42 5.750172265577456E-4 4.791810221314547E-5 0.0 2.036711016467535 0.0 43 5.750172265577456E-4 4.791810221314547E-5 0.0 2.170737948357703 0.0 44 5.750172265577456E-4 4.791810221314547E-5 0.0 2.307160785358528 0.0 45 5.750172265577456E-4 4.791810221314547E-5 0.0 2.452160962655506 0.0 46 5.750172265577456E-4 4.791810221314547E-5 0.0 2.61187199733192 0.0 47 6.229353287708911E-4 4.791810221314547E-5 0.0 2.7728768207680887 0.0 48 6.229353287708911E-4 4.791810221314547E-5 0.0 2.9409735233318033 0.0 49 6.229353287708911E-4 4.791810221314547E-5 0.0 3.10849520866896 0.0 50 6.229353287708911E-4 4.791810221314547E-5 0.0 3.274914777655214 0.0 51 6.229353287708911E-4 4.791810221314547E-5 0.0 3.4596390616868895 0.0 52 6.229353287708911E-4 4.791810221314547E-5 0.0 3.6440279190030735 0.0 53 6.229353287708911E-4 4.791810221314547E-5 0.0 3.843223469903119 0.0 54 6.229353287708911E-4 4.791810221314547E-5 0.0 4.06321547716367 0.0 55 6.229353287708911E-4 4.791810221314547E-5 0.0 4.295666190999639 0.0 56 6.708534309840366E-4 4.791810221314547E-5 0.0 4.546325783676602 0.0 57 6.708534309840366E-4 4.791810221314547E-5 0.0 4.787353837808724 0.0 58 7.18771533197182E-4 4.791810221314547E-5 0.0 5.0410322709251165 0.0 59 7.18771533197182E-4 4.791810221314547E-5 0.0 5.296819100538887 0.0 60 7.18771533197182E-4 4.791810221314547E-5 0.0 5.5563435421252825 0.0 61 7.18771533197182E-4 4.791810221314547E-5 0.0 5.823391125759143 0.0 62 7.18771533197182E-4 4.791810221314547E-5 0.0 6.108455915825145 0.0 63 7.18771533197182E-4 4.791810221314547E-5 0.0 6.4057398219555 0.0 64 7.18771533197182E-4 4.791810221314547E-5 0.0 6.7081030469204475 0.0 65 7.18771533197182E-4 4.791810221314547E-5 0.0 7.036869146204839 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTATA 170 0.0 29.232746 10 GCGGTAT 225 7.2759576E-12 22.085377 9 CGCGGAA 250 1.8189894E-12 21.30223 59 CGATCGT 265 3.6379788E-12 20.088737 1 ATTCGCG 285 0.0 19.93 56 TACGTTA 90 0.0048428746 19.736116 71 TCGGCGA 90 0.004868463 19.718142 13 CGCTCCG 275 5.456968E-12 19.36056 25 TCGCGGG 185 1.9533036E-7 19.19442 63 GACGTAT 355 0.0 18.9988 39 GCGTAAG 395 0.0 18.871775 38 GCGCGAG 245 5.493348E-10 18.835546 24 ACGGATA 95 0.006656414 18.679 30 TGCGTAA 400 0.0 18.634983 37 TTGCGTA 405 0.0 18.4036 36 ACGATCG 195 3.4029472E-7 18.207035 59 CGGTTAC 275 1.3096724E-10 18.068123 4 CGCGCTT 315 1.8189894E-12 18.030176 38 TCGCGCT 300 2.3646862E-11 17.747604 37 CGGAGCG 200 4.4573608E-7 17.744627 35 >>END_MODULE