FastQCFastQC Report
Thu 26 May 2016
SRR936110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936110_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086894
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132680.6357773801640141No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95080.4556053158425871No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67330.32263258220110846No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21030.10077176895424492No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA16500.048.52434100-101
ACGTATC5000.046.431843100-101
CGTACAT19200.043.405834100-101
CGGTATC12550.041.503586100-101
AGGTATC24550.040.857304100-101
TACGCAG10650.040.80323100-101
GGTATCA106900.040.6896551
AGTATCA33600.040.12825100-101
CCGTACA7500.039.685337100-101
TACCGTA1850.036.99812798-99
CGAGTAC12450.036.33838100-101
TCGAGTA4800.035.964836100-101
CCTGTCG7500.034.9159896-97
CCGTATC6600.034.724667100-101
ACGTACA6450.033.68639100-101
TGTATCA28950.033.516632100-101
CACGCAG10700.033.380188100-101
AACGTAT2700.033.0659698-99
ACCGTAT2800.032.94786598-99
TCGTATC6600.032.46982100-101
Produced by FastQC (version 0.11.5)