FastQCFastQC Report
Thu 26 May 2016
SRR936109_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936109_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2789019
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80510.28866780756961496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA2700.028.92554559
TCGCGCT3200.025.51391837
CTCGCGC3300.024.73988236
CGCGCTT3400.022.96740538
AATTCGC3750.022.71766755
CGATCGT3800.022.4167391
TCGCGGA3800.021.48540558
CCTCGCG3500.021.29742835
CGGTTAC2700.021.0322344
CGGTATA1601.0385356E-619.96490710
CAATTCG4100.019.0485354
CGCTCCG4350.018.76784925
AACTCGA4350.018.76751113
ATTCGCG4300.018.16125156
GACGCTC4950.017.2128223
TGCGACG1250.001606220117.036113
GACGTAT4600.016.97590639
GTTACGG3008.110874E-915.3803731
GTCGGGT3703.45608E-1115.35858766
ACTCGAG5400.015.11827414
Produced by FastQC (version 0.11.5)