##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936109_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2789019 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.42587734253514 39.0 36.0 41.0 30.0 41.0 2 36.28705361992873 39.0 36.0 41.0 29.0 41.0 3 36.19495743843982 39.0 36.0 41.0 29.0 41.0 4 36.07030213849386 39.0 35.0 41.0 28.0 41.0 5 36.000858724877816 39.0 35.0 41.0 28.0 41.0 6 35.895652198855586 39.0 35.0 40.0 28.0 41.0 7 35.72291511818313 39.0 35.0 40.0 27.0 41.0 8 35.54816406772417 38.0 35.0 40.0 27.0 41.0 9 35.44573127683964 38.0 35.0 40.0 27.0 41.0 10-11 35.65197350753078 39.0 35.0 40.0 27.0 41.0 12-13 35.82359245311703 39.0 35.0 41.0 28.0 41.0 14-15 35.76701001319819 39.0 35.0 41.0 27.0 41.0 16-17 35.59761604349056 39.0 35.0 41.0 27.0 41.0 18-19 35.43662323562514 39.0 35.0 41.0 26.5 41.0 20-21 35.22362719651605 39.0 35.0 41.0 25.5 41.0 22-23 34.94769236064724 39.0 34.0 41.0 24.0 41.0 24-25 34.71156865550216 39.0 34.0 41.0 24.0 41.0 26-27 33.772411195477694 38.0 33.0 39.5 22.5 40.5 28-29 33.80331829937336 38.0 33.0 39.5 22.5 40.5 30-31 34.176987679180385 38.0 33.0 40.0 22.5 41.0 32-33 34.01851923561654 38.0 33.0 40.0 22.0 41.0 34-35 33.74606232514013 38.0 33.0 40.0 20.0 41.0 36-37 33.46028047854819 37.5 33.0 40.0 19.0 41.0 38-39 33.18285013476064 37.0 32.5 40.0 18.0 41.0 40-41 32.80044524616003 36.5 32.0 40.0 15.5 41.0 42-43 32.44139624003996 36.0 32.0 39.0 13.0 41.0 44-45 32.070089160382196 35.5 31.0 39.0 9.5 40.5 46-47 31.6776280477114 35.0 31.0 38.5 7.5 40.0 48-49 31.211744344516834 35.0 31.0 37.5 4.5 40.0 50-51 30.7738453915158 35.0 30.5 37.0 2.0 39.0 52-53 30.320636754356997 35.0 30.0 36.5 2.0 39.0 54-55 29.854951328764702 34.0 29.5 36.0 2.0 38.5 56-57 29.397847056617397 34.0 29.0 35.5 2.0 37.0 58-59 28.96611048544309 34.0 29.0 35.0 2.0 37.0 60-61 28.61273103553615 34.0 29.0 35.0 2.0 36.5 62-63 28.27067707319312 34.0 28.5 35.0 2.0 36.0 64-65 27.89113663262961 34.0 27.5 35.0 2.0 36.0 66-67 27.50547970451259 34.0 26.5 35.0 2.0 35.0 68-69 27.138143375860828 33.0 26.0 35.0 2.0 35.0 70-71 26.712709199901468 33.0 25.0 35.0 2.0 35.0 72-73 26.225734389045037 33.0 24.5 35.0 2.0 35.0 74-75 25.638051228765384 33.0 22.5 35.0 2.0 35.0 76-77 23.940977275522325 31.0 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1256277390434661 1101 2 1.1744207342343103 1101 3 1.1504070717560708 1101 4 1.086777408929521 1101 5 1.1564115480178287 1101 6 1.2309345826051157 1101 7 1.0971625518948827 1101 8 1.2840032637860546 1101 9 1.25346844989582 1101 10-11 1.1675027557556206 1101 12-13 1.2459723645624123 1101 14-15 1.2321720033025088 1101 16-17 1.280186403372852 1101 18-19 1.3452336540676484 1101 20-21 1.3073867551180456 1101 22-23 1.3551084032526788 1101 24-25 1.3892725414814109 1101 26-27 1.1716588608900764 1101 28-29 1.2646110923070353 1101 30-31 1.3783738998931838 1101 32-33 1.4588571664790493 1101 34-35 1.3611216941320876 1101 36-37 1.3464363250753664 1101 38-39 1.3453880342885327 1101 40-41 1.4477844370351107 1101 42-43 1.3664889362795236 1101 44-45 1.3803512472481145 1101 46-47 1.3667381398265377 1101 48-49 1.3573344750259402 1101 50-51 1.245715622636414 1101 52-53 1.4467488089614413 1101 54-55 1.3201456209171454 1101 56-57 1.273531863333627 1101 58-59 1.2828299020849983 1101 60-61 1.3690453388961643 1101 62-63 1.1985670275233815 1101 64-65 1.1926235498570819 1101 66-67 1.230913376204768 1101 68-69 1.2711693709309095 1101 70-71 1.325271728030593 1101 72-73 1.4789550174704722 1101 74-75 1.5087506197550873 1101 76-77 1.4662266229939611 1102 1 0.9227125416340769 1102 2 1.0831664772199403 1102 3 1.0349464398108594 1102 4 1.0833877777275518 1102 5 1.0460196840975584 1102 6 1.0647547620969746 1102 7 1.0678298195813767 1102 8 1.1262675205441397 1102 9 0.9095475191794407 1102 10-11 0.8492064509722397 1102 12-13 0.9626293324977979 1102 14-15 0.9917410328916816 1102 16-17 0.9331828590644804 1102 18-19 0.9760803683837267 1102 20-21 1.1529217708043618 1102 22-23 1.1826808912184603 1102 24-25 1.0674026059905373 1102 26-27 0.9335464651577183 1102 28-29 0.9503715205788836 1102 30-31 1.181002356573778 1102 32-33 1.1304436779057099 1102 34-35 1.1132530280191943 1102 36-37 1.233239015191856 1102 38-39 1.3148405735721624 1102 40-41 1.368953802511946 1102 42-43 1.2180029936608392 1102 44-45 1.2698313148477638 1102 46-47 1.1463704272547233 1102 48-49 1.144829292737537 1102 50-51 1.2800434025666654 1102 52-53 1.1714632366031275 1102 54-55 1.2053901570891412 1102 56-57 1.265557546378087 1102 58-59 1.3023823181997685 1102 60-61 1.2355535845765822 1102 62-63 1.301405187859995 1102 64-65 1.3066720364781084 1102 66-67 1.3153023636722985 1102 68-69 1.274581766512508 1102 70-71 1.2940206821505704 1102 72-73 1.2176139090713214 1102 74-75 1.117859612891099 1102 76-77 1.2494702304998775 1103 1 0.6860524455803727 1103 2 0.513246803718026 1103 3 0.5123789501573128 1103 4 0.6071902005027212 1103 5 0.6787642784351604 1103 6 0.5864516706889802 1103 7 0.6986451893821126 1103 8 0.768341278441703 1103 9 0.6139088767304628 1103 10-11 0.7080710430156572 1103 12-13 0.7300130311391015 1103 14-15 0.8445310278505218 1103 16-17 0.8198613689339851 1103 18-19 0.7969519757890993 1103 20-21 0.8796441417793588 1103 22-23 0.7416689463874633 1103 24-25 0.5042800454437923 1103 26-27 0.48547342570083174 1103 28-29 0.4431925473453333 1103 30-31 0.757676579948452 1103 32-33 0.7754321013510221 1103 34-35 0.8266140625737819 1103 36-37 0.7308868766233658 1103 38-39 0.7677196189516522 1103 40-41 0.7444984439406142 1103 42-43 0.7026259481424901 1103 44-45 0.7767787569876319 1103 46-47 0.3821822803185029 1103 48-49 0.41915424526913725 1103 50-51 0.5960635732416115 1103 52-53 0.6470819860937311 1103 54-55 0.679417620842603 1103 56-57 0.6967993016675287 1103 58-59 0.5386042559972921 1103 60-61 0.5643968648922133 1103 62-63 0.5517788977608937 1103 64-65 0.43212611815696533 1103 66-67 0.5468256088507495 1103 68-69 0.511059686536175 1103 70-71 0.5885855878325899 1103 72-73 0.5981474871172239 1103 74-75 0.6211079911169115 1103 76-77 0.8896100849401698 1104 1 0.39859568061110195 1104 2 0.4972778636936823 1104 3 0.13095413949945822 1104 4 0.24861261687587444 1104 5 0.32580182058454454 1104 6 0.3568861277321105 1104 7 0.48972419021424685 1104 8 0.5517447776183744 1104 9 0.5747529325079057 1104 10-11 0.4234460478570412 1104 12-13 0.5615950547556636 1104 14-15 0.5502911897698937 1104 16-17 0.5863803195800301 1104 18-19 0.46942937972722376 1104 20-21 0.4585325951066537 1104 22-23 0.6016610945420453 1104 24-25 0.5096294819066429 1104 26-27 0.2886055667429872 1104 28-29 0.43138763812275727 1104 30-31 0.5814039706828069 1104 32-33 0.4580123010860149 1104 34-35 0.6452268973481878 1104 36-37 0.4584318017374187 1104 38-39 0.41906907419320305 1104 40-41 0.5444172260477984 1104 42-43 0.5730753401232747 1104 44-45 0.42903032354229254 1104 46-47 0.15111224044897398 1104 48-49 0.4232199812945545 1104 50-51 0.3786061862277883 1104 52-53 0.2609933083609306 1104 54-55 0.32420479944802594 1104 56-57 0.3497979085224614 1104 58-59 0.41156970083543953 1104 60-61 0.19225225479091534 1104 62-63 0.13533547271315172 1104 64-65 0.13317194523562037 1104 66-67 0.21375889398540693 1104 68-69 0.27601736403306987 1104 70-71 0.20772795270514877 1104 72-73 0.00946109850838539 1104 74-75 -0.06360315134419992 1104 76-77 -0.052167424980265054 1105 1 0.18787196826589536 1105 2 0.43567023226115964 1105 3 0.2492750266144057 1105 4 0.17431122219433348 1105 5 0.20176460148665143 1105 6 0.07140118505062532 1105 7 0.16153258589363162 1105 8 0.19942962005568887 1105 9 0.14835365261053823 1105 10-11 0.23344281751156615 1105 12-13 0.32953381844152574 1105 14-15 0.21745064697988425 1105 16-17 0.420081904633534 1105 18-19 0.5389999277133768 1105 20-21 0.48947177494377314 1105 22-23 0.5578430676048711 1105 24-25 0.5130115731472316 1105 26-27 0.4329669469856867 1105 28-29 0.5581100100470309 1105 30-31 0.5156006262980881 1105 32-33 0.6331683003318815 1105 34-35 0.664629088005249 1105 36-37 0.6726407809482495 1105 38-39 0.6910931902741169 1105 40-41 0.6824774208341822 1105 42-43 0.558152810935205 1105 44-45 0.5082646052100301 1105 46-47 0.46386773663464 1105 48-49 0.5369267050114956 1105 50-51 0.5844886913204697 1105 52-53 0.39838185569411166 1105 54-55 0.3976704428270814 1105 56-57 0.4771341610458073 1105 58-59 0.5136175789238102 1105 60-61 0.30427483042238634 1105 62-63 0.1743028082874183 1105 64-65 0.34880152922230323 1105 66-67 0.3932729856490482 1105 68-69 0.1779197616161703 1105 70-71 0.014674000187778091 1105 72-73 0.02863998147561375 1105 74-75 -0.15962861934194095 1105 76-77 0.0949486582315977 1106 1 -0.010345919318574204 1106 2 0.21210214994331977 1106 3 -0.233986734072694 1106 4 0.013275091950710305 1106 5 -0.08949656431092023 1106 6 0.09366306657465628 1106 7 0.19020812013231136 1106 8 0.21802224156652272 1106 9 0.1266157851035956 1106 10-11 -0.02196928291322564 1106 12-13 0.007951296109929729 1106 14-15 -0.03039868795221423 1106 16-17 0.21043969815109165 1106 18-19 0.292857995437835 1106 20-21 0.019825083552014178 1106 22-23 0.23707096606466393 1106 24-25 0.2798654208741027 1106 26-27 0.23256760415270605 1106 28-29 0.09948562236495917 1106 30-31 0.0968396464034953 1106 32-33 0.049446313586763324 1106 34-35 0.056346413233207215 1106 36-37 0.1480452361130986 1106 38-39 0.051749032739934364 1106 40-41 0.054881169567714494 1106 42-43 0.06722255243712283 1106 44-45 -0.0509884831031826 1106 46-47 0.07901836452194289 1106 48-49 0.08350701974523744 1106 50-51 -0.05867983902354368 1106 52-53 -0.02732899081509288 1106 54-55 0.11574799690160731 1106 56-57 0.1265618122546428 1106 58-59 0.02199657467592786 1106 60-61 -0.07891187193765248 1106 62-63 -0.1764083867898698 1106 64-65 -0.04594891926862843 1106 66-67 -0.027402213964276 1106 68-69 -0.17828312359803533 1106 70-71 -0.1815210306256425 1106 72-73 -0.18678613138608213 1106 74-75 -0.32483827142451815 1106 76-77 -0.24623384736873177 1107 1 0.1158281440457003 1107 2 0.19107909100705456 1107 3 0.11674722775217816 1107 4 0.19648240080795887 1107 5 0.25138558452730564 1107 6 0.14117347863701468 1107 7 0.1395961619222703 1107 8 0.2388850284113886 1107 9 0.19134658984944508 1107 10-11 0.36650531199203584 1107 12-13 0.2901376576860173 1107 14-15 0.29747836479586454 1107 16-17 0.21987937286060344 1107 18-19 0.4309738760964237 1107 20-21 0.16529077520200275 1107 22-23 0.1521140342685996 1107 24-25 -0.09381310761440176 1107 26-27 -0.007503188311240194 1107 28-29 0.1619565906220899 1107 30-31 0.41156763935827456 1107 32-33 0.5600015664061502 1107 34-35 0.5266939674810871 1107 36-37 0.530613593565981 1107 38-39 0.5132961026849188 1107 40-41 0.48282649260315225 1107 42-43 0.515140660146713 1107 44-45 0.5550310835885597 1107 46-47 0.41694419622162116 1107 48-49 0.46819033615158645 1107 50-51 0.41468849267332786 1107 52-53 0.3922762787063263 1107 54-55 0.2973990496924266 1107 56-57 0.38113062128116937 1107 58-59 0.3281520492893897 1107 60-61 0.20359967327651063 1107 62-63 0.4018810151196739 1107 64-65 0.3247932557979816 1107 66-67 0.40785348469270133 1107 68-69 0.31544566846776334 1107 70-71 0.287072387758748 1107 72-73 0.4687319242491377 1107 74-75 0.4696789187414119 1107 76-77 0.6007621193981443 1108 1 0.02565721196700821 1108 2 0.16567424380205154 1108 3 0.02326905559007031 1108 4 -0.04405160625057647 1108 5 -0.015776632525046352 1108 6 -0.01593276029984736 1108 7 -0.2257932592300378 1108 8 0.14521403059060844 1108 9 0.10382747730620423 1108 10-11 0.008504340662788934 1108 12-13 8.478295195502028E-4 1108 14-15 0.1266490331061334 1108 16-17 0.12567530410586158 1108 18-19 -0.0788998377657677 1108 20-21 -0.10722275393160174 1108 22-23 -0.18282913550253 1108 24-25 -0.18084035470674564 1108 26-27 -0.3806699777053524 1108 28-29 -0.12247972503698179 1108 30-31 -0.1338437367154981 1108 32-33 -0.005856663795682948 1108 34-35 -0.0650277709582312 1108 36-37 -0.008058885785281689 1108 38-39 -0.05706979400373058 1108 40-41 0.046662653734983905 1108 42-43 -0.028221643263790952 1108 44-45 -0.12143159771222756 1108 46-47 -0.16084875542713917 1108 48-49 -0.021530896087050166 1108 50-51 0.029753337301986704 1108 52-53 0.10498778351723104 1108 54-55 0.1781986547598713 1108 56-57 0.13363922175893705 1108 58-59 0.07315231732505723 1108 60-61 0.11093880987095162 1108 62-63 0.20482812728206312 1108 64-65 0.19160119904163153 1108 66-67 0.05292845600221696 1108 68-69 0.20670353184745238 1108 70-71 -0.14665165978784245 1108 72-73 -0.10150655548456555 1108 74-75 -0.19729756692961686 1108 76-77 -0.2047201967820591 1109 1 0.10119892579929513 1109 2 0.0804961762532912 1109 3 -0.14137158607073985 1109 4 0.17053736290006327 1109 5 0.07543051253036737 1109 6 0.11428991087494467 1109 7 -0.20569739361351935 1109 8 0.09069013013448313 1109 9 0.09498038079276228 1109 10-11 -0.13902901869661122 1109 12-13 -0.03568378764726532 1109 14-15 0.05304298580684019 1109 16-17 0.21514091871209473 1109 18-19 -0.03109746882881126 1109 20-21 0.042730287913329335 1109 22-23 0.07055354671126679 1109 24-25 0.08262204248455873 1109 26-27 0.02858729418739614 1109 28-29 0.21419356114927268 1109 30-31 0.27961050305236057 1109 32-33 0.23321331577367488 1109 34-35 0.3460651860600876 1109 36-37 0.3571104257758577 1109 38-39 0.3791085643518812 1109 40-41 0.3814577461442923 1109 42-43 0.4493826538973664 1109 44-45 0.41797617334597703 1109 46-47 0.15710165485417704 1109 48-49 0.3776285185065795 1109 50-51 0.3518841622452662 1109 52-53 0.3203119801831775 1109 54-55 0.46284042468769115 1109 56-57 0.5475393388762306 1109 58-59 0.5025588223935209 1109 60-61 0.4933759036882073 1109 62-63 0.34568272862507143 1109 64-65 0.3588389736329525 1109 66-67 0.37655344017734294 1109 68-69 0.45443627134860876 1109 70-71 0.31233874782377313 1109 72-73 0.2359947886363436 1109 74-75 0.13095701989953668 1109 76-77 0.245960895104961 1110 1 -0.38330976198734135 1110 2 -0.5582703604121946 1110 3 -0.6240286362859209 1110 4 -0.4199555932310375 1110 5 -0.3932769399476683 1110 6 -0.5145957881622039 1110 7 -0.6875941637357101 1110 8 -0.5461513661212081 1110 9 -0.28647046708648105 1110 10-11 -0.43458350832514725 1110 12-13 -0.39041969799793463 1110 14-15 -0.46875217745585473 1110 16-17 -0.6445504478397623 1110 18-19 -0.4587908305105941 1110 20-21 -0.6481458922029617 1110 22-23 -0.689393557368355 1110 24-25 -0.7480225720664322 1110 26-27 -0.5974706114767017 1110 28-29 -0.5558299940254514 1110 30-31 -0.4852057834062933 1110 32-33 -0.3669862736801619 1110 34-35 -0.19443865306127606 1110 36-37 -0.16604957151231758 1110 38-39 -0.09101405916751304 1110 40-41 -0.10201578321199634 1110 42-43 -0.20968537473177662 1110 44-45 -0.12507841440788425 1110 46-47 -0.21418455657556024 1110 48-49 -0.23086508193832245 1110 50-51 -0.21911919338587538 1110 52-53 -0.16424156489355823 1110 54-55 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-1.0417033109946559 2314 28-29 -1.13809814127535 2314 30-31 -1.2662391791508938 2314 32-33 -1.2773116087964382 2314 34-35 -1.3657088387400762 2314 36-37 -1.356856642754785 2314 38-39 -1.3118269435973637 2314 40-41 -1.239745424239203 2314 42-43 -1.058136384857825 2314 44-45 -1.2364222436798222 2314 46-47 -1.0684056424298163 2314 48-49 -1.0207049057462037 2314 50-51 -1.133101709758769 2314 52-53 -1.0175516920845702 2314 54-55 -1.1231242506655512 2314 56-57 -1.0150032657576453 2314 58-59 -1.1519424888423977 2314 60-61 -1.1331176700805088 2314 62-63 -1.1663574462418644 2314 64-65 -1.0006089767542967 2314 66-67 -1.0014988478217752 2314 68-69 -0.9202643042824015 2314 70-71 -0.7907869742651741 2314 72-73 -0.5888801527649612 2314 74-75 -0.6204901516849866 2314 76-77 -0.5813921557748962 2315 1 -1.2968721732259922 2315 2 -1.2556725848043158 2315 3 -1.391154765863412 2315 4 -1.3816018773043268 2315 5 -1.1487692330012464 2315 6 -1.2474829875667837 2315 7 -1.1447546003931635 2315 8 -1.097857712307551 2315 9 -1.058326020477871 2315 10-11 -1.1073026077675223 2315 12-13 -1.2625301574221481 2315 14-15 -1.4918925802232934 2315 16-17 -1.3495702929741853 2315 18-19 -1.1454563448652593 2315 20-21 -1.3427874049306325 2315 22-23 -1.358210382327428 2315 24-25 -1.4705461654396075 2315 26-27 -1.469158090703914 2315 28-29 -1.4352953642551256 2315 30-31 -1.4735603377689799 2315 32-33 -1.5441795039331723 2315 34-35 -1.4525106416035172 2315 36-37 -1.6593648720228167 2315 38-39 -1.636599930274535 2315 40-41 -1.690651794079848 2315 42-43 -1.831365759500791 2315 44-45 -1.6430036379585573 2315 46-47 -1.542480502114202 2315 48-49 -1.4275761341152666 2315 50-51 -1.3704803189367176 2315 52-53 -1.3779779193481687 2315 54-55 -1.3167550272862378 2315 56-57 -1.551637396934364 2315 58-59 -1.4540646467568017 2315 60-61 -1.4733037699405607 2315 62-63 -1.5869468335443813 2315 64-65 -1.6194547019680137 2315 66-67 -1.5456787660509654 2315 68-69 -1.57836445164045 2315 70-71 -1.6664495523913132 2315 72-73 -3.2648829562714745 2315 74-75 -3.8291120565206427 2315 76-77 -3.5318125695221774 2316 1 -3.3018191373400967 2316 2 -3.2139921258834647 2316 3 -2.669363367065259 2316 4 -2.744073308397155 2316 5 -3.342647883786917 2316 6 -2.8751795199859984 2316 7 -3.2663526621999637 2316 8 -3.195590425784431 2316 9 -2.6942187266643316 2316 10-11 -2.6754742254532786 2316 12-13 -2.6547541752162402 2316 14-15 -3.012538651026162 2316 16-17 -4.199817144978873 2316 18-19 -4.042207636748628 2316 20-21 -3.5512947020735197 2316 22-23 -3.4207537999167634 2316 24-25 -3.579696357698829 2316 26-27 -3.179544015583133 2316 28-29 -3.2719545783025907 2316 30-31 -3.474038824947037 2316 32-33 -3.7622299738728913 2316 34-35 -3.7743169050903127 2316 36-37 -3.877872380451297 2316 38-39 -3.903875622336237 2316 40-41 -3.8171024350968707 2316 42-43 -4.113505553307391 2316 44-45 -3.8553578802425257 2316 46-47 -3.53874485539567 2316 48-49 -3.6890387511840252 2316 50-51 -3.9683805669518613 2316 52-53 -5.215118501740903 2316 54-55 -4.285579357398856 2316 56-57 -4.166444761027041 2316 58-59 -4.198950841718773 2316 60-61 -3.921171450095141 2316 62-63 -3.989406838141445 2316 64-65 -3.950998670393833 2316 66-67 -3.9545791931253156 2316 68-69 -3.9680401303847574 2316 70-71 -3.8224453894248143 2316 72-73 -3.830665712848486 2316 74-75 -3.797811536053789 2316 76-77 -3.798311520243761 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 73444.0 3 6454.0 4 8401.0 5 9923.0 6 11179.0 7 11947.0 8 13783.0 9 14654.0 10 18099.0 11 19757.0 12 15737.0 13 13041.0 14 13371.0 15 13729.0 16 14305.0 17 14874.0 18 15888.0 19 16685.0 20 17995.0 21 19238.0 22 21354.0 23 24204.0 24 28087.0 25 33074.0 26 39029.0 27 45895.0 28 54523.0 29 63395.0 30 75292.0 31 90302.0 32 109689.0 33 137591.0 34 181136.0 35 240650.0 36 336747.0 37 451389.0 38 406851.0 39 106503.0 40 804.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.494919717734604 26.907548723914136 28.812757667219007 21.78477389113225 2 22.125093683995367 27.026138282956147 28.80281715394298 22.04595087910551 3 22.269513719176008 27.212433412285307 28.414212790220233 22.10384007831845 4 21.952525368801062 27.080076727690926 28.896424430791058 22.07097347271695 5 22.25869958115784 27.13114636525331 28.662609730908255 21.94754432268059 6 22.330176812047373 27.013680808857384 28.690110602555148 21.966031776540095 7 22.25674425876896 26.70399063027939 28.756127272994597 22.28313783795705 8 22.13440152626313 27.15830317983439 28.481231912383315 22.22606338151916 9 22.10743371908295 26.949553704308038 28.650246906053773 22.29276567055524 10-11 22.554211198638953 26.960500579892887 28.39518857456806 22.090099646900104 12-13 22.715425532868647 26.69006960275614 28.295193289389175 22.299311574986042 14-15 22.30642086464113 26.988762630767514 28.46589838443431 22.238918120157038 16-17 22.47280755414644 26.856121536445805 28.56280743223134 22.10826347717642 18-19 22.46611105532684 26.825448625077104 28.251473900133405 22.456966419462653 20-21 22.32741794198894 27.04173349985968 28.23611475972776 22.39473379842362 22-23 22.53634270727819 27.14117185697064 27.843379432908343 22.479106002842826 24-25 22.511898522365172 27.30571693898084 27.611331850922898 22.571052687731097 26-27 22.51523978851235 27.23207425199738 27.63250927746502 22.620176682025253 28-29 22.75295050805789 27.225014585351108 27.591844682057275 22.430190224533725 30-31 22.53370926927362 27.159202272827514 27.610063637305725 22.697024820593143 32-33 22.48638832943694 27.442386509945614 27.528645603143982 22.54257955747346 34-35 22.535647388708984 27.068581296055804 27.719590908707865 22.676180406527344 36-37 22.531319023459304 27.254753637069573 27.836785174924128 22.377142164546996 38-39 22.44191656985871 27.194594126471866 27.932026020975066 22.43146328269436 40-41 22.370737995834926 27.11031343822811 27.810235533637478 22.708713032299478 42-43 22.319210669474295 27.41752017416681 27.525155176933442 22.73811397942545 44-45 22.397790312805927 27.100457930955347 27.819215887371218 22.68253586886751 46-47 22.266968064522956 27.126255361162784 27.908082261100773 22.698694313213487 48-49 22.375948834357743 27.0531142818769 27.70902060372144 22.861916280043918 50-51 22.24614225248373 27.350440379434172 27.578435889466746 22.824981478615356 52-53 22.282636280011715 27.142619123670748 27.73936063844688 22.835383957870654 54-55 22.26800611142593 27.161540337563032 27.66354520088085 22.90690835013019 56-57 22.185259640321323 27.410414303084686 27.588006837599483 22.81631921899451 58-59 22.284407203185605 27.287035815106382 27.621260414036858 22.807296567671155 60-61 22.197861469823042 27.160957828841916 27.52245410064264 23.1187266006924 62-63 22.20126741421355 27.004146469744473 27.735996676081687 23.058589439960294 64-65 22.240600713664875 26.94975241936482 27.631444220073032 23.178202646897276 66-67 22.1206037143597 27.09674249320172 27.821007486534356 22.961646305904225 68-69 22.19150742269672 26.857171758223092 27.827494781343727 23.123826037736457 70-71 21.982383872301458 26.67147383724319 27.911257478692846 23.4348848117625 72-73 22.098311250477064 26.533591163532037 27.854327971281013 23.513769614709886 74-75 21.958326313075336 26.57493768792087 27.737815389015598 23.7289206099882 76-77 21.779025017589777 26.427607185493784 28.13748970677244 23.655878090144 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8067.0 1 4874.0 2 1630.0 3 1576.5 4 1574.0 5 1663.0 6 1784.0 7 1911.5 8 2087.0 9 2167.0 10 2210.5 11 2300.5 12 2542.5 13 2738.0 14 2811.5 15 3091.5 16 3543.5 17 3789.0 18 4089.5 19 4909.5 20 6020.0 21 7814.0 22 9017.0 23 10255.5 24 13448.5 25 18106.0 26 20809.0 27 23774.5 28 31509.0 29 39592.5 30 42907.0 31 48848.5 32 61054.5 33 72942.5 34 83476.5 35 88387.0 36 94981.5 37 105738.0 38 111041.0 39 112182.0 40 115110.5 41 120968.0 42 127480.5 43 131064.0 44 129962.5 45 129133.0 46 131012.0 47 133089.0 48 133559.0 49 132099.5 50 129557.0 51 123382.5 52 118291.0 53 115329.5 54 107441.5 55 96264.5 56 84241.0 57 78468.0 58 74722.5 59 63916.0 60 52660.5 61 48466.0 62 42409.5 63 30583.0 64 21396.0 65 17979.0 66 15436.0 67 11385.0 68 8352.0 69 5741.0 70 4655.0 71 3949.5 72 2765.0 73 2033.0 74 1780.0 75 1521.5 76 1019.5 77 624.5 78 473.0 79 392.0 80 277.0 81 197.5 82 132.0 83 112.0 84 112.0 85 92.5 86 71.0 87 69.0 88 59.0 89 52.0 90 45.5 91 36.0 92 35.5 93 32.5 94 34.0 95 36.0 96 34.0 97 27.0 98 31.5 99 83.5 100 124.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010613050681978143 2 0.01394755647057263 3 0.01448537998486206 4 0.016349834834398762 5 0.01358900746104634 6 0.010577195781025514 7 0.016564964240114534 8 0.018250144584888092 9 0.018250144584888092 10-11 0.02054485824585634 12-13 0.017210352457261855 14-15 0.008730668381965128 16-17 0.006919995883857371 18-19 0.017891595575361803 20-21 0.024076565989690282 22-23 0.021459158220148374 24-25 0.019451283766801157 26-27 0.024255840494453427 28-29 0.009393984049588761 30-31 0.0088203056343467 32-33 0.011742480061985952 34-35 0.012567142783896418 36-37 0.01213688397246487 38-39 0.015148695652485696 40-41 0.012602997684849047 42-43 0.015345897607725152 44-45 0.01394755647057263 46-47 0.024309622845882367 48-49 0.02378972678206925 50-51 0.0367692009269209 52-53 0.04046225572504167 54-55 0.02864806586115046 56-57 0.021996981734437808 58-59 0.01703107795249871 60-61 0.021817707229674663 62-63 0.02665811885827956 64-65 0.026371279650658528 66-67 0.029150034474487267 68-69 0.04247013017838889 70-71 0.05437395729466167 72-73 0.08714533676536446 74-75 0.08750388577489075 76-77 0.09320481502635873 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2789019.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.54526317379242 #Duplication Level Percentage of deduplicated Percentage of total 1 74.4286027232001 37.620133123017915 2 13.048655355692034 13.190954380351396 3 4.923712922930521 7.466110964451778 4 2.385738391277018 4.823510994036681 5 1.3886488388667497 3.509481050825056 6 0.8363942688946138 2.5365461062997974 7 0.5411330628769971 1.9146199152610728 8 0.4092956460954006 1.6550364918223541 9 0.30194898634738787 1.3735881881989715 >10 1.536900528604347 14.16046475333032 >50 0.12846724066240303 4.507102263935274 >100 0.06787037580484538 5.9523580477227735 >500 0.0022760291871906264 0.7834393858915075 >1k 2.845036483921093E-4 0.21721460946534676 >5k 7.112591209802732E-5 0.28943972538985546 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8051 0.28866780756961496 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 7.170980190525773E-5 0.0 2 0.0 0.0 0.0 4.6611371238417524E-4 0.0 3 0.0 0.0 0.0 6.095333161946906E-4 3.5854900952628867E-5 4 0.0 0.0 0.0 0.0011115019295314947 3.5854900952628867E-5 5 0.0 0.0 0.0 0.002366423462873505 3.5854900952628867E-5 6 0.0 0.0 0.0 0.004768701826699639 3.5854900952628867E-5 7 0.0 0.0 0.0 0.00975253305911505 7.170980190525773E-5 8 0.0 0.0 0.0 0.01513076820200938 7.170980190525773E-5 9 0.0 0.0 0.0 0.0227320072039667 7.170980190525773E-5 10 0.0 0.0 0.0 0.030835214819260822 1.0756470285788659E-4 11 0.0 0.0 0.0 0.04008577926503907 1.0756470285788659E-4 12 0.0 0.0 0.0 0.05328038281560649 1.0756470285788659E-4 13 0.0 0.0 0.0 0.06998876665953155 1.0756470285788659E-4 14 0.0 0.0 0.0 0.09074875431110366 1.0756470285788659E-4 15 0.0 0.0 0.0 0.11337319681221246 1.0756470285788659E-4 16 0.0 0.0 0.0 0.1344200236714056 1.0756470285788659E-4 17 0.0 0.0 0.0 0.15518001132297773 1.0756470285788659E-4 18 0.0 0.0 0.0 0.17852155184313911 1.0756470285788659E-4 19 0.0 0.0 0.0 0.2037275472128372 1.0756470285788659E-4 20 0.0 0.0 0.0 0.230797997432072 1.0756470285788659E-4 21 0.0 0.0 0.0 0.2600914515103698 1.0756470285788659E-4 22 0.0 0.0 0.0 0.29250428197154626 1.0756470285788659E-4 23 0.0 0.0 0.0 0.32452270852224385 1.0756470285788659E-4 24 0.0 0.0 0.0 0.3609871427910674 1.0756470285788659E-4 25 0.0 0.0 0.0 0.3953719928046385 1.0756470285788659E-4 26 0.0 0.0 0.0 0.4329120741020409 1.0756470285788659E-4 27 0.0 0.0 0.0 0.4704521553994433 1.0756470285788659E-4 28 0.0 0.0 0.0 0.5100718209520982 1.0756470285788659E-4 29 0.0 0.0 0.0 0.5487951139809374 1.0756470285788659E-4 30 0.0 7.170980190525773E-5 0.0 0.5882355050288292 1.0756470285788659E-4 31 0.0 1.0756470285788659E-4 0.0 0.6348468762672467 1.0756470285788659E-4 32 0.0 1.0756470285788659E-4 0.0 0.6846134787894955 1.0756470285788659E-4 33 0.0 1.0756470285788659E-4 0.0 0.7404395595727387 1.0756470285788659E-4 34 0.0 1.792745047631443E-4 0.0 0.794795589416924 1.0756470285788659E-4 35 0.0 1.792745047631443E-4 0.0 0.8517690270306513 1.0756470285788659E-4 36 0.0 1.792745047631443E-4 0.0 0.9144792487967992 1.0756470285788659E-4 37 0.0 1.792745047631443E-4 0.0 0.9770101960581838 1.0756470285788659E-4 38 0.0 1.792745047631443E-4 0.0 1.040186531536716 1.0756470285788659E-4 39 0.0 1.792745047631443E-4 0.0 1.1056934355771688 1.0756470285788659E-4 40 0.0 1.792745047631443E-4 0.0 1.1757539120386058 1.0756470285788659E-4 41 0.0 1.792745047631443E-4 0.0 1.2514794628505579 1.0756470285788659E-4 42 0.0 1.792745047631443E-4 0.0 1.3314358919749203 1.0756470285788659E-4 43 0.0 1.792745047631443E-4 0.0 1.421395838465066 1.0756470285788659E-4 44 0.0 1.792745047631443E-4 0.0 1.5174152632162061 1.0756470285788659E-4 45 0.0 1.792745047631443E-4 0.0 1.6139725114816357 1.0756470285788659E-4 46 0.0 1.792745047631443E-4 0.0 1.7203898575090382 1.0756470285788659E-4 47 0.0 1.792745047631443E-4 0.0 1.8268789133383458 1.0756470285788659E-4 48 0.0 1.792745047631443E-4 0.0 1.936989314163869 1.792745047631443E-4 49 0.0 1.792745047631443E-4 0.0 2.0502908011741763 1.792745047631443E-4 50 0.0 1.792745047631443E-4 0.0 2.1667116645673623 1.792745047631443E-4 51 0.0 1.792745047631443E-4 0.0 2.2922755277034685 1.792745047631443E-4 52 0.0 1.792745047631443E-4 0.0 2.426587986672016 1.792745047631443E-4 53 0.0 1.792745047631443E-4 0.0 2.5668165042977478 1.792745047631443E-4 54 0.0 1.792745047631443E-4 0.0 2.7207415940873836 1.792745047631443E-4 55 0.0 1.792745047631443E-4 0.0 2.880941291543729 1.792745047631443E-4 56 0.0 1.792745047631443E-4 0.0 3.0539411886401635 1.792745047631443E-4 57 0.0 1.792745047631443E-4 0.0 3.225542744599445 2.1512940571577317E-4 58 0.0 1.792745047631443E-4 0.0 3.4043511356502054 2.1512940571577317E-4 59 0.0 1.792745047631443E-4 0.0 3.582155589474292 2.1512940571577317E-4 60 0.0 1.792745047631443E-4 0.0 3.7672744430927145 2.1512940571577317E-4 61 0.0 1.792745047631443E-4 0.0 3.9651217865493207 2.1512940571577317E-4 62 0.0 1.792745047631443E-4 0.0 4.1749805218250575 2.1512940571577317E-4 63 0.0 1.792745047631443E-4 0.0 4.390611896154168 2.1512940571577317E-4 64 0.0 1.792745047631443E-4 0.0 4.62087207007195 2.8683920762103093E-4 65 0.0 1.792745047631443E-4 0.0 4.861566020166948 2.8683920762103093E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAA 270 0.0 28.925545 59 TCGCGCT 320 0.0 25.513918 37 CTCGCGC 330 0.0 24.739882 36 CGCGCTT 340 0.0 22.967405 38 AATTCGC 375 0.0 22.717667 55 CGATCGT 380 0.0 22.416739 1 TCGCGGA 380 0.0 21.485405 58 CCTCGCG 350 0.0 21.297428 35 CGGTTAC 270 0.0 21.032234 4 CGGTATA 160 1.0385356E-6 19.964907 10 CAATTCG 410 0.0 19.04853 54 CGCTCCG 435 0.0 18.767849 25 AACTCGA 435 0.0 18.767511 13 ATTCGCG 430 0.0 18.161251 56 GACGCTC 495 0.0 17.21282 23 TGCGACG 125 0.0016062201 17.03611 3 GACGTAT 460 0.0 16.975906 39 GTTACGG 300 8.110874E-9 15.380373 1 GTCGGGT 370 3.45608E-11 15.358587 66 ACTCGAG 540 0.0 15.118274 14 >>END_MODULE