FastQCFastQC Report
Thu 26 May 2016
SRR936109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936109_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2789019
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82350.2952651093448987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61470.22040007615580964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44530.15966187394205633No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12350.047.71455100-101
GGTATCA83700.038.104981
CGTACAT20550.036.93014100-101
CGGTATC11800.036.571148100-101
GCGTATC3750.034.919983100-101
TACGCAG10850.032.367184100-101
GTATCAA163700.031.2965811
CGAGTAC11350.030.941315100-101
GCGTACA6650.029.985119100-101
TCGAGTA5300.029.199814100-101
CCGGTAT4850.028.840895100-101
CCGTACA8000.028.645298100-101
ACGTATC5750.027.94979100-101
CCTGTCG8900.027.75148496-97
TCGTACA10900.027.303991100-101
AGGTATC22800.026.759108100-101
TCGTATC4950.026.454533100-101
AGTATCA35450.026.361252100-101
GTCGTAC3900.025.94579198-99
GGTACAT23350.025.746437100-101
Produced by FastQC (version 0.11.5)