FastQCFastQC Report
Thu 26 May 2016
SRR936108_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936108_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793448
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79430.28434393623937154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC2400.026.621034
CGATCGT4200.024.5073741
TCGCGCT4100.024.24159237
TAACGCG1502.1318556E-823.6699252
AATTCGC4350.023.66610555
ATTCGCG4200.023.66525856
CGCGGAA4200.022.82129759
TCGCGGA4400.022.59077858
CTCGCGC4500.022.08559636
CGCGCTT4650.021.37698638
AACGCGA1858.469215E-921.11025253
TATCGCG850.003483234720.89233666
CCTCGCG5000.020.58545735
CGGTATA1407.3090177E-620.28450810
CAATTCG5150.019.30085854
CGCTCCG5650.018.84742425
ATCGTTC5500.018.0693863
AACTCGA5750.017.9023213
ACGCGAA2206.194023E-817.74989754
ACTCGAG5850.017.59598414
Produced by FastQC (version 0.11.5)