##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936108_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2793448 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.410223136424946 39.0 36.0 41.0 30.0 41.0 2 36.27423563996896 39.0 36.0 41.0 29.0 41.0 3 36.133239637895535 39.0 36.0 41.0 29.0 41.0 4 36.029009310357665 39.0 35.0 41.0 28.0 41.0 5 35.95652433838038 39.0 35.0 41.0 28.0 41.0 6 35.85820820720486 39.0 35.0 40.0 27.0 41.0 7 35.713237547289225 39.0 35.0 40.0 27.0 41.0 8 35.58323441137977 38.0 35.0 40.0 27.0 41.0 9 35.744299875995544 39.0 35.0 40.0 28.0 41.0 10-11 35.95377021516062 39.0 35.0 41.0 29.0 41.0 12-13 35.86267974202491 39.0 35.0 41.0 27.5 41.0 14-15 35.70877353006034 39.0 35.0 41.0 27.0 41.0 16-17 35.557664040998795 39.0 35.0 41.0 27.0 41.0 18-19 35.39459155853268 39.0 35.0 41.0 26.0 41.0 20-21 35.18332326214771 39.0 35.0 41.0 25.5 41.0 22-23 34.8932414349578 39.0 34.0 41.0 24.0 41.0 24-25 34.65863549276736 39.0 34.0 40.5 23.5 41.0 26-27 33.697945334940904 38.0 33.0 39.5 22.0 40.5 28-29 33.70737167829864 38.0 33.0 39.5 22.0 40.5 30-31 34.11487326773221 38.0 33.0 40.0 22.0 41.0 32-33 33.997669367749104 38.0 33.0 40.0 21.5 41.0 34-35 33.74682650258748 38.0 33.0 40.0 20.0 41.0 36-37 33.44039892634479 37.5 33.0 40.0 18.5 41.0 38-39 33.130437545284536 37.0 32.5 40.0 18.0 41.0 40-41 32.771577455531656 36.5 32.0 40.0 14.5 41.0 42-43 32.400767438663614 36.0 32.0 39.0 12.5 41.0 44-45 32.042314730755685 35.5 31.0 39.0 8.0 40.5 46-47 31.6222465927413 35.0 31.0 38.5 7.5 40.0 48-49 31.20277270240935 35.0 31.0 37.5 4.0 40.0 50-51 30.748442068726533 35.0 30.5 37.0 2.0 39.0 52-53 30.260454821424993 35.0 30.0 36.5 2.0 39.0 54-55 29.795574322486047 34.0 29.5 36.0 2.0 38.5 56-57 29.35452261864191 34.0 29.0 35.5 2.0 37.0 58-59 28.92081166357849 34.0 29.0 35.0 2.0 37.0 60-61 28.560611831686145 34.0 29.0 35.0 2.0 36.5 62-63 28.189725385974608 34.0 28.5 35.0 2.0 36.0 64-65 27.808492407948883 34.0 27.0 35.0 2.0 36.0 66-67 27.452273856538586 34.0 26.5 35.0 2.0 35.0 68-69 27.078174356565793 33.0 26.0 35.0 2.0 35.0 70-71 26.623100018328604 33.0 25.0 35.0 2.0 35.0 72-73 26.143224609872817 33.0 24.5 35.0 2.0 35.0 74-75 25.567557190969726 33.0 21.5 35.0 2.0 35.0 76-77 23.81457270727789 31.0 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.2173175117552972 1101 2 1.0364381823956776 1101 3 1.1922964579888742 1101 4 1.1021580162186666 1101 5 1.2712527104599474 1101 6 1.1138364786516348 1101 7 1.1616360077437733 1101 8 1.2805813943544848 1101 9 1.2845597638032658 1101 10-11 1.2344525472341346 1101 12-13 1.2733059076431275 1101 14-15 1.390348834407682 1101 16-17 1.2465648988955564 1101 18-19 1.3908592766831944 1101 20-21 1.3801980733517425 1101 22-23 1.2352935153907225 1101 24-25 1.2721689999896952 1101 26-27 1.2481655824343107 1101 28-29 1.2363395482451338 1101 30-31 1.2924450083822236 1101 32-33 1.3922890340551888 1101 34-35 1.2776758419359524 1101 36-37 1.295290360991416 1101 38-39 1.2322691739940907 1101 40-41 1.1833707383934282 1101 42-43 1.3097751227330363 1101 44-45 1.1917228393841057 1101 46-47 1.2442921983882087 1101 48-49 1.1849170843996824 1101 50-51 1.1879326800349546 1101 52-53 1.2461806227744425 1101 54-55 1.3012107710826832 1101 56-57 1.2172322337979793 1101 58-59 1.2711056058855306 1101 60-61 1.1946995230907547 1101 62-63 1.1353805744260903 1101 64-65 1.2192747429060056 1101 66-67 1.2662609527375643 1101 68-69 1.1521624907170853 1101 70-71 1.1139609645163873 1101 72-73 1.172402611416846 1101 74-75 1.3441915680165408 1101 76-77 1.4994976750519875 1102 1 0.6896781587023924 1102 2 0.8766584132755781 1102 3 0.8336714660134064 1102 4 0.6032248067463186 1102 5 0.5985858851907935 1102 6 0.739069529899119 1102 7 0.5955595688982456 1102 8 0.5312202980336806 1102 9 0.6564916553644835 1102 10-11 0.7907824101940335 1102 12-13 0.8988378112883382 1102 14-15 0.8023643321362712 1102 16-17 0.7537832756368772 1102 18-19 0.8724819311601237 1102 20-21 0.8854769682700478 1102 22-23 0.7655179379077381 1102 24-25 0.9322061582863057 1102 26-27 0.7551552694964982 1102 28-29 0.8617839939483289 1102 30-31 1.0173673498080618 1102 32-33 0.9669524380421493 1102 34-35 0.9136766425008815 1102 36-37 0.8824048908672069 1102 38-39 0.7774609130703496 1102 40-41 0.9082272220252747 1102 42-43 0.8915107625996583 1102 44-45 0.7487423965810649 1102 46-47 0.6966514858854254 1102 48-49 0.7168686879840998 1102 50-51 0.7322465165913137 1102 52-53 0.8560068020028027 1102 54-55 0.9262396027488009 1102 56-57 0.8925955278960807 1102 58-59 0.9936566135777518 1102 60-61 0.8018089172670244 1102 62-63 0.9124846440903411 1102 64-65 0.8150916401127155 1102 66-67 0.8423760981420578 1102 68-69 0.846497738608992 1102 70-71 0.6987517414042301 1102 72-73 0.6959150522713387 1102 74-75 0.726406942639386 1102 76-77 0.68827881495071 1103 1 0.5529125553736804 1103 2 0.5970689541909735 1103 3 0.5908864452089659 1103 4 0.6140948945872822 1103 5 0.5196029910622713 1103 6 0.6378989335052268 1103 7 0.6236427866513665 1103 8 0.6010899235537934 1103 9 0.5886673372877382 1103 10-11 0.7449923038602364 1103 12-13 0.7661831649637918 1103 14-15 0.6670874019467163 1103 16-17 0.6396806404080237 1103 18-19 0.5646335723991456 1103 20-21 0.5685819151031453 1103 22-23 0.6600274154851746 1103 24-25 0.7080896534319407 1103 26-27 0.6614909144340899 1103 28-29 0.4859633744383913 1103 30-31 0.6367737298358023 1103 32-33 0.7037124935206478 1103 34-35 0.8435721579886248 1103 36-37 0.7928403971085416 1103 38-39 0.7084512043324835 1103 40-41 0.7260654097275392 1103 42-43 0.8259980487762633 1103 44-45 0.7934913563591479 1103 46-47 0.7541549532918026 1103 48-49 0.6484285585342597 1103 50-51 0.7801078203361129 1103 52-53 0.8383073105557415 1103 54-55 0.8301878228135244 1103 56-57 0.8321147142113823 1103 58-59 0.8649834744191764 1103 60-61 0.6805625002536146 1103 62-63 0.4993158969798479 1103 64-65 0.586449938771981 1103 66-67 0.6774810449751634 1103 68-69 0.7600973687523087 1103 70-71 0.6702616813025202 1103 72-73 0.6054887924470194 1103 74-75 0.6961008862362448 1103 76-77 0.6750231515202891 1104 1 0.3033533686185734 1104 2 0.23917821374983816 1104 3 0.3993718035652236 1104 4 0.37294556599967166 1104 5 0.27661965503250485 1104 6 0.26352110898923087 1104 7 0.3492112491439485 1104 8 0.3072729575828106 1104 9 0.1806073685663847 1104 10-11 0.23104116326810242 1104 12-13 0.4262959838172691 1104 14-15 0.5451537342554005 1104 16-17 0.44152629645073205 1104 18-19 0.4207677470202995 1104 20-21 0.3454930945239525 1104 22-23 0.3398211921124812 1104 24-25 0.26041581424733806 1104 26-27 0.09035873517507298 1104 28-29 0.25177149610155425 1104 30-31 0.43020003625871084 1104 32-33 0.443076566216547 1104 34-35 0.4697068718968396 1104 36-37 0.44817383829929014 1104 38-39 0.4377014874581917 1104 40-41 0.3410174671076831 1104 42-43 0.3499415314921137 1104 44-45 0.29972378908308883 1104 46-47 0.2804721619419652 1104 48-49 0.14749537620130226 1104 50-51 0.0996384248769111 1104 52-53 0.11977330850644918 1104 54-55 -0.059204400667031365 1104 56-57 -0.16482537099597394 1104 58-59 -0.20460504955213565 1104 60-61 -0.09513420470243261 1104 62-63 -0.17281568906340183 1104 64-65 -0.2780050037939432 1104 66-67 -0.4164752727727752 1104 68-69 -0.4811675829814952 1104 70-71 -0.3576106155210823 1104 72-73 -0.19259609133661826 1104 74-75 -0.28869272860493567 1104 76-77 -0.3665185623339937 1105 1 0.5064891729740211 1105 2 0.6622740796807562 1105 3 0.6669686224831395 1105 4 0.7252461530150427 1105 5 0.4438001035618271 1105 6 0.6746999501312985 1105 7 0.6124192348140269 1105 8 0.6814512494036293 1105 9 0.7349884922879113 1105 10-11 0.6565497891226926 1105 12-13 0.7269818518217832 1105 14-15 0.5978051117140879 1105 16-17 0.7210450373157897 1105 18-19 0.506328937572647 1105 20-21 0.5288573219300758 1105 22-23 0.4912833016795446 1105 24-25 0.500685650466778 1105 26-27 0.28706499046924705 1105 28-29 0.42504135107011365 1105 30-31 0.6132593805823845 1105 32-33 0.4900280711972371 1105 34-35 0.4554150751406567 1105 36-37 0.5176892798788515 1105 38-39 0.4625164378387794 1105 40-41 0.5501879696484693 1105 42-43 0.6655106330538629 1105 44-45 0.5468767211796504 1105 46-47 0.4983040584490652 1105 48-49 0.37644536110406435 1105 50-51 0.3624816422147532 1105 52-53 0.2914832786449786 1105 54-55 0.22097111908236755 1105 56-57 0.2626530318364324 1105 58-59 0.2169676602358308 1105 60-61 0.27850693881480737 1105 62-63 0.10915358980345857 1105 64-65 0.2813133649430455 1105 66-67 0.3793591204455282 1105 68-69 0.18255393269738462 1105 70-71 0.26186717390726955 1105 72-73 -0.09639316597014869 1105 74-75 -0.38449793475990646 1105 76-77 -0.366731728637852 1106 1 0.18414965447181686 1106 2 0.0894930963543743 1106 3 0.16477576694438767 1106 4 0.24001573703707635 1106 5 0.2721483065362378 1106 6 0.16103122444924622 1106 7 -0.05489032610812217 1106 8 0.03383959002479031 1106 9 0.16864734333572784 1106 10-11 -0.060164926159153254 1106 12-13 0.3461423943783828 1106 14-15 0.30336994993042055 1106 16-17 0.3493157329956773 1106 18-19 0.3764684977775943 1106 20-21 0.35338395007477885 1106 22-23 0.4481022682877125 1106 24-25 0.26657900718613803 1106 26-27 0.3166970620406886 1106 28-29 0.38053654625475275 1106 30-31 0.6415814570357625 1106 32-33 0.5379162397184203 1106 34-35 0.5128662149464915 1106 36-37 0.5480836865049881 1106 38-39 0.6507492992148158 1106 40-41 0.6221620353034325 1106 42-43 0.7059969103306045 1106 44-45 0.5971662589686773 1106 46-47 0.49237470888174784 1106 48-49 0.4470753024017746 1106 50-51 0.40174120525526646 1106 52-53 0.3348111439157577 1106 54-55 0.3495502932406218 1106 56-57 0.38658329133218317 1106 58-59 0.34743148836513527 1106 60-61 0.12293160568435724 1106 62-63 0.16167454294103223 1106 64-65 0.15158909596642545 1106 66-67 0.06301374860573716 1106 68-69 0.22059785792818687 1106 70-71 0.1928405453472024 1106 72-73 0.04686289636576291 1106 74-75 0.34995235465326857 1106 76-77 0.29281221888425435 1107 1 0.09071552877766464 1107 2 -0.019303492951365797 1107 3 0.11265772974006438 1107 4 0.04282051490382344 1107 5 0.10865661179035868 1107 6 -0.11528919345604294 1107 7 -0.2088399542303634 1107 8 -0.1488922771766923 1107 9 -0.07128540376547221 1107 10-11 0.05189725438924597 1107 12-13 0.010108058219621796 1107 14-15 -0.1762139338558839 1107 16-17 -0.11182653588704738 1107 18-19 -0.07351441189359775 1107 20-21 -0.17398001366547788 1107 22-23 -0.06759224944957509 1107 24-25 -0.07283364509164869 1107 26-27 -0.2887736053457388 1107 28-29 -0.2035230531899117 1107 30-31 0.04398047915417891 1107 32-33 0.201003712204745 1107 34-35 0.15043643427170394 1107 36-37 0.2642228756310985 1107 38-39 0.25441159324137885 1107 40-41 0.230284831165541 1107 42-43 0.19541341650779742 1107 44-45 0.15067351593526013 1107 46-47 0.16173764787485467 1107 48-49 0.07929304058923847 1107 50-51 0.08064735426275504 1107 52-53 0.13412595691957918 1107 54-55 0.1723436264385434 1107 56-57 0.12373739713402898 1107 58-59 0.03723650526781341 1107 60-61 -0.062936582346655 1107 62-63 0.026797008834023472 1107 64-65 -0.07204024419353061 1107 66-67 0.048154982249027256 1107 68-69 -0.03579823630281709 1107 70-71 -0.1289029533666195 1107 72-73 -0.047243206798480486 1107 74-75 -0.17326022630383875 1107 76-77 -0.18145698291735002 1108 1 0.0937520615465175 1108 2 0.2598696596583494 1108 3 0.17384235349820898 1108 4 0.14953052068957362 1108 5 0.18104919917514906 1108 6 0.03142274302903303 1108 7 0.09402021169545804 1108 8 0.16702995882716465 1108 9 0.12682606744646563 1108 10-11 0.047771845306449734 1108 12-13 0.21515670993733949 1108 14-15 0.21769763122360786 1108 16-17 0.19251309190714494 1108 18-19 0.16326496569720916 1108 20-21 0.15291348476805666 1108 22-23 0.05756533435643263 1108 24-25 0.23330305731199985 1108 26-27 0.10033394794378836 1108 28-29 0.22479935327437062 1108 30-31 0.2384950702238129 1108 32-33 0.1644391249139474 1108 34-35 0.20901131142777984 1108 36-37 0.30185627012441785 1108 38-39 0.4835340967487056 1108 40-41 0.22265094764640736 1108 42-43 0.2868375549491091 1108 44-45 0.26261304948084074 1108 46-47 0.23263819844171252 1108 48-49 0.3064955779346157 1108 50-51 0.20103198259360866 1108 52-53 0.3817923193740853 1108 54-55 0.32119311381881843 1108 56-57 0.345271680701682 1108 58-59 0.35277888888125375 1108 60-61 0.36004045439823784 1108 62-63 0.4081624578264069 1108 64-65 0.40395963894835063 1108 66-67 0.41891314374059263 1108 68-69 0.34890865623026457 1108 70-71 0.4373093003501367 1108 72-73 0.57079390708547 1108 74-75 0.6877552072239013 1108 76-77 0.626430558927698 1109 1 -0.29896852405916263 1109 2 -0.27447406557829623 1109 3 -0.3080810803284635 1109 4 -0.2412005264626771 1109 5 -0.19798403948099974 1109 6 -0.46549243713054267 1109 7 -0.3100145798170999 1109 8 -0.3185966140281522 1109 9 -0.3662843321740752 1109 10-11 -0.34382428403425536 1109 12-13 -0.31005264334032034 1109 14-15 -0.1401049546117079 1109 16-17 -0.16067835740668102 1109 18-19 -0.21116842993860985 1109 20-21 -0.10731908606572205 1109 22-23 -0.10900627867551549 1109 24-25 -0.1622881480621956 1109 26-27 -0.13607253527877106 1109 28-29 -0.03930368863016298 1109 30-31 0.0914495442384009 1109 32-33 0.08492365375849431 1109 34-35 0.04861987867775497 1109 36-37 0.1334955782845384 1109 38-39 0.18624416057581783 1109 40-41 0.2745577612038872 1109 42-43 0.2491761755232318 1109 44-45 0.15402151847735723 1109 46-47 0.12725552756633363 1109 48-49 0.020748729291998558 1109 50-51 0.06768641463402503 1109 52-53 0.168679623408309 1109 54-55 0.284830298800717 1109 56-57 0.24662708906527442 1109 58-59 0.278622748698794 1109 60-61 0.3791183776739544 1109 62-63 0.3031424965631153 1109 64-65 0.5051910070242585 1109 66-67 0.33306634761276044 1109 68-69 0.2970560514783358 1109 70-71 0.25436474579405655 1109 72-73 0.1603684997194712 1109 74-75 0.16499390854655616 1109 76-77 0.36704876518090757 1110 1 -0.3952992138868723 1110 2 -0.32720824286671046 1110 3 -0.5258619094174364 1110 4 -0.3967324379717567 1110 5 -0.43078028419905223 1110 6 -0.47131632007588564 1110 7 -0.28699561830411113 1110 8 -0.6263831697485642 1110 9 -0.5668053688678967 1110 10-11 -0.4713553539020481 1110 12-13 -0.4046910123494101 1110 14-15 -0.3128520095902516 1110 16-17 -0.30596144244137236 1110 18-19 -0.4710609688319778 1110 20-21 -0.5611135939947971 1110 22-23 -0.6705393673255031 1110 24-25 -0.7321962544271443 1110 26-27 -0.6490729647761313 1110 28-29 -0.45663050864329335 1110 30-31 -0.3233505442640805 1110 32-33 -0.17204031329313096 1110 34-35 -0.40910554235980356 1110 36-37 -0.3352662618151925 1110 38-39 -0.35779811816073703 1110 40-41 -0.3030728016545723 1110 42-43 -0.2661655207165765 1110 44-45 -0.35024344813174935 1110 46-47 -0.4965709468243418 1110 48-49 -0.5385822815741399 1110 50-51 -0.4351027874842508 1110 52-53 -0.4805538973420518 1110 54-55 -0.45080811214582184 1110 56-57 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-0.6677400263223703 2314 22-23 -0.5456278592736723 2314 24-25 -0.4787346667636001 2314 26-27 -0.5740993260617415 2314 28-29 -0.800808918346732 2314 30-31 -0.8436021594750116 2314 32-33 -0.9375840016126844 2314 34-35 -0.873649613212983 2314 36-37 -1.357354007326009 2314 38-39 -0.9627470846989823 2314 40-41 -0.8239755313051553 2314 42-43 -0.7063851732568196 2314 44-45 -0.8028232523440906 2314 46-47 -0.5850489225756874 2314 48-49 -0.535575107826876 2314 50-51 -0.5018086390065584 2314 52-53 -0.37562713141536364 2314 54-55 -0.36565696789835656 2314 56-57 -0.3431513152068 2314 58-59 -0.34451240092454327 2314 60-61 -0.24554670493166597 2314 62-63 -0.18930843194512548 2314 64-65 -0.2963520340794865 2314 66-67 -0.3495698912563405 2314 68-69 -0.2806888038285429 2314 70-71 -0.26237207540822993 2314 72-73 -0.3136756263910918 2314 74-75 -0.34969615448655134 2314 76-77 -0.2413190421857898 2315 1 -1.2390012377297666 2315 2 -1.1660000113262683 2315 3 -1.074327840505319 2315 4 -0.960787699829794 2315 5 -1.1936687659163852 2315 6 -1.0386306231450249 2315 7 -1.4277192823141505 2315 8 -1.3546145099437439 2315 9 -1.009654501792724 2315 10-11 -1.1528287142021583 2315 12-13 -1.0919473769081307 2315 14-15 -1.0567865717807052 2315 16-17 -0.815964679789019 2315 18-19 -0.9688056722642386 2315 20-21 -0.9370305643714048 2315 22-23 -1.0678945877988895 2315 24-25 -1.3393783268636312 2315 26-27 -1.0003760806397963 2315 28-29 -1.076686461357994 2315 30-31 -1.2255279943021335 2315 32-33 -1.171531348843878 2315 34-35 -1.1745797303529173 2315 36-37 -1.2455746275220037 2315 38-39 -1.271607908968008 2315 40-41 -1.3554464950343643 2315 42-43 -1.329218289483176 2315 44-45 -1.2955948505373982 2315 46-47 -1.108221893998838 2315 48-49 -1.0353390933540823 2315 50-51 -0.9380522781860527 2315 52-53 -1.0341278290173292 2315 54-55 -1.1169591394196416 2315 56-57 -1.3186016240480605 2315 58-59 -1.0537398424937976 2315 60-61 -0.9972548232275003 2315 62-63 -1.074317928964323 2315 64-65 -0.9252602418519906 2315 66-67 -0.957520597060963 2315 68-69 -1.1509256197534334 2315 70-71 -0.98886065038878 2315 72-73 -0.9273010269290047 2315 74-75 -0.9970563387227074 2315 76-77 -1.3068491801710103 2316 1 -2.355694227218308 2316 2 -1.9438460232324104 2316 3 -2.321163511559213 2316 4 -3.140567673062513 2316 5 -2.4407665609279974 2316 6 -2.491243539354805 2316 7 -1.9440862939519548 2316 8 -1.8946660568477824 2316 9 -2.1491135574507894 2316 10-11 -2.1711537876631226 2316 12-13 -2.8247443016516343 2316 14-15 -4.198060514920098 2316 16-17 -3.328528409452673 2316 18-19 -2.8497788271269044 2316 20-21 -2.6009686020774936 2316 22-23 -2.872378066949892 2316 24-25 -2.8068020748801032 2316 26-27 -2.7377647297200056 2316 28-29 -3.25039234336991 2316 30-31 -3.3193121573771904 2316 32-33 -3.2759960279511517 2316 34-35 -3.3003393929128393 2316 36-37 -3.2224949857332277 2316 38-39 -3.367041734470476 2316 40-41 -3.0711522544733114 2316 42-43 -3.3224864037808146 2316 44-45 -3.2305518754594686 2316 46-47 -3.2414978471693416 2316 48-49 -3.045834611158636 2316 50-51 -3.124644989732097 2316 52-53 -3.0984417847784265 2316 54-55 -3.2678843583117825 2316 56-57 -3.006130714424092 2316 58-59 -3.3110443220390167 2316 60-61 -3.105645460805725 2316 62-63 -3.1658281770586463 2316 64-65 -3.16013096877597 2316 66-67 -3.1382792906318464 2316 68-69 -3.13868778512094 2316 70-71 -3.004145210169604 2316 72-73 -2.9360836288550587 2316 74-75 -3.1043247710475157 2316 76-77 -3.365800878966148 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 73553.0 3 6565.0 4 8513.0 5 10507.0 6 11524.0 7 12164.0 8 14098.0 9 14899.0 10 18092.0 11 20058.0 12 15797.0 13 13506.0 14 13514.0 15 14013.0 16 14534.0 17 15071.0 18 15953.0 19 16827.0 20 18068.0 21 19294.0 22 21743.0 23 24607.0 24 28638.0 25 33473.0 26 39434.0 27 46003.0 28 53958.0 29 63546.0 30 75035.0 31 89712.0 32 109016.0 33 137270.0 34 180328.0 35 239186.0 36 336245.0 37 454356.0 38 409268.0 39 104367.0 40 713.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.443397121393016 26.86813216317954 28.910185385726532 21.778285329700918 2 22.18519206693066 26.968025063555707 28.767669130132656 22.07911373938098 3 22.29515450991961 27.244863691726273 28.390092347217514 22.0698894511366 4 21.919188411069193 27.09641915099531 28.919909156691816 22.064483281243678 5 22.245024657420462 27.1745753961406 28.60894483998634 21.971455106452602 6 22.3244590201498 27.018631761353056 28.6923038366248 21.964605381872342 7 22.224263034164622 26.725506497300756 28.815297926320245 22.234932542214374 8 22.056989614979063 27.156373244920722 28.57916228810601 22.207474851994206 9 22.12084919897529 26.879485568307743 28.726792302858765 22.2728729298582 10-11 22.502235142345537 27.0085578113257 28.384929286433714 22.104277759895048 12-13 22.675698842495123 26.68220962840112 28.32070132020291 22.321390208900844 14-15 22.30492434548751 27.00476223890386 28.459872892399606 22.230440523209023 16-17 22.457336772072324 26.869044618640793 28.571027096537684 22.102591512749207 18-19 22.42601193475714 26.842140594553904 28.278142316653256 22.4537051540357 20-21 22.3409216175252 27.002765880091733 28.274540853343193 22.381771649039873 22-23 22.487549791435846 27.138563539788663 27.841851067954803 22.532035600820688 24-25 22.525592198065414 27.283156709167105 27.634954881213066 22.556296211554415 26-27 22.562720369834647 27.213741195947627 27.63681189474378 22.586726539473947 28-29 22.75071820961428 27.25315723138495 27.595055259492547 22.401069299508222 30-31 22.52460315783934 27.156573426047682 27.666465347218764 22.652358068894213 32-33 22.49686022299935 27.465727382865197 27.51101607050285 22.526396323632603 34-35 22.54730042027713 27.06367740875289 27.710544018828877 22.678478152141107 36-37 22.56981296153456 27.218538766799533 27.856410337192656 22.35523793447326 38-39 22.430280974194726 27.216328450854803 27.918544640566672 22.434845934383798 40-41 22.347908011845533 27.156830505522407 27.804045690116126 22.691215792515933 42-43 22.27766808944243 27.44647405160267 27.509130367923262 22.766727491031645 44-45 22.381132513913354 27.09275861149149 27.863585317180767 22.66252355741439 46-47 22.250827747140367 27.121265963055492 27.875641453684203 22.75226483611994 48-49 22.377645787389657 27.09985164955066 27.715898954135596 22.806603608924085 50-51 22.208175516777416 27.358949821273033 27.600439364872475 22.832435297077076 52-53 22.285497587156712 27.182301805227898 27.73068830132681 22.801512306288576 54-55 22.302544039439628 27.135030171259316 27.696328029551076 22.86609775974998 56-57 22.192493456926705 27.37256671070486 27.60242460696806 22.83251522540037 58-59 22.300663002118707 27.302744114479978 27.608546792804457 22.788046090596865 60-61 22.231346604924017 27.15077932015054 27.533913880388116 23.08396019453733 62-63 22.208995530754862 27.00539091616929 27.72853028382204 23.057083269253802 64-65 22.223061664387046 26.959997994886965 27.63799474373939 23.1789455969866 66-67 22.11437603623374 27.063627996540475 27.82338504185574 22.998610925370045 68-69 22.202214833991295 26.828314108873176 27.807999736444387 23.16147132069114 70-71 22.003869071924193 26.65696354146519 27.900353309808 23.438814076802615 72-73 22.142611633685576 26.5285056873818 27.882186199938424 23.446696478994202 74-75 21.914587076768306 26.5280872490014 27.819468196528792 23.737857477701503 76-77 21.7423570429559 26.39700529640372 28.20893854108152 23.65169911955886 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8044.0 1 4841.0 2 1554.0 3 1486.0 4 1502.0 5 1656.5 6 1869.5 7 1974.0 8 2059.0 9 2098.0 10 2133.5 11 2288.5 12 2521.0 13 2634.0 14 2737.5 15 2970.0 16 3371.5 17 3644.0 18 4054.0 19 4958.0 20 6059.5 21 7813.0 22 8959.0 23 10364.5 24 13763.0 25 18335.0 26 20914.0 27 23955.5 28 31643.0 29 39351.5 30 42414.0 31 48889.0 32 61398.0 33 73397.5 34 84224.5 35 89086.0 36 95207.0 37 105836.0 38 111233.0 39 112122.0 40 115287.0 41 121137.5 42 127812.0 43 131801.0 44 130303.5 45 129164.0 46 130848.0 47 132801.0 48 133428.0 49 132307.5 50 129975.5 51 123705.0 52 118646.0 53 115878.5 54 108005.5 55 96458.0 56 84279.5 57 78543.0 58 74829.5 59 63983.0 60 52478.0 61 48106.0 62 41968.5 63 30286.0 64 21381.0 65 18021.0 66 15581.5 67 11542.0 68 8352.0 69 5717.0 70 4672.0 71 3953.0 72 2710.5 73 1983.0 74 1779.0 75 1521.0 76 1028.0 77 645.5 78 498.0 79 395.5 80 259.0 81 178.0 82 114.5 83 98.0 84 91.5 85 68.5 86 52.0 87 52.0 88 53.0 89 50.0 90 38.0 91 30.0 92 32.0 93 31.5 94 36.5 95 34.5 96 25.0 97 24.0 98 24.0 99 45.0 100 65.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0029712384121701923 2 0.008197754173337037 3 0.010524627628651044 4 0.018149612951449248 5 0.013388471881345206 6 0.013173683562393143 7 0.015822739496135243 8 0.017290459675641 9 0.01657449861246746 10-11 0.020082707822017806 12-13 0.0194741409183203 14-15 0.008018763907543651 16-17 0.006300457355927155 18-19 0.01236822736632291 20-21 0.010900507186817152 22-23 0.015124677459541038 24-25 0.023394027739195432 26-27 0.01489199011400964 28-29 0.007786076562012252 30-31 0.007481793110163498 32-33 0.009164301608621316 34-35 0.010417233469175013 36-37 0.009039008422565947 38-39 0.015518456044286488 40-41 0.011723862409466724 42-43 0.015518456044286488 44-45 0.019241453572788895 46-47 0.017021974276950923 48-49 0.01430132223689147 50-51 0.027940380490347413 52-53 0.0319318634175399 54-55 0.025917790486882163 56-57 0.014963586220326995 58-59 0.01684298401115754 60-61 0.020225900034652515 62-63 0.024861747918701188 64-65 0.02104925525730209 66-67 0.020816567911770684 68-69 0.03109060916831099 70-71 0.05598815514017086 72-73 0.0670676525927814 74-75 0.07777127048722582 76-77 0.07589187269639529 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2793448.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.696589798538895 #Duplication Level Percentage of deduplicated Percentage of total 1 74.66986229410259 37.85507379037505 2 12.81054781670426 12.989021755160476 3 4.916632910771912 7.477695656021997 4 2.427757251616564 4.923160540625324 5 1.3546148539172516 3.433717679202529 6 0.8440952653587966 2.5675650851270255 7 0.5724578680106917 2.0315163198038904 8 0.3967807787729611 1.6092345905118093 9 0.28055995042291626 1.2801089448440095 >10 1.528715837744756 14.09287929639739 >50 0.12945675106073798 4.578810431761905 >100 0.06596481688887834 5.894746828696969 >500 0.002198937318362217 0.7628374304472393 >1k 2.8373384752250133E-4 0.2179946496615119 >5k 7.093346188062533E-5 0.2856370013628158 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7943 0.28434393623937154 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.5798053158677017E-5 0.0 2 0.0 0.0 0.0 2.5058637211073916E-4 0.0 3 0.0 0.0 0.0 3.937785847454472E-4 0.0 4 0.0 0.0 0.0 0.0010023454884429566 0.0 5 0.0 0.0 0.0 0.00239846956163136 0.0 6 0.0 3.5798053158677017E-5 0.0 0.005119121601690813 0.0 7 0.0 3.5798053158677017E-5 0.0 0.01091840621339649 0.0 8 0.0 3.5798053158677017E-5 0.0 0.017218863569323645 0.0 9 0.0 3.5798053158677017E-5 0.0 0.023376128712616094 0.0 10 0.0 3.5798053158677017E-5 0.0 0.030786325716462237 3.5798053158677017E-5 11 0.0 3.5798053158677017E-5 0.0 0.038912483783481916 3.5798053158677017E-5 12 0.0 3.5798053158677017E-5 0.0 0.051907177080081676 3.5798053158677017E-5 13 0.0 3.5798053158677017E-5 0.0 0.06826688737359707 3.5798053158677017E-5 14 0.0 3.5798053158677017E-5 0.0 0.08698926917558515 7.159610631735403E-5 15 0.0 3.5798053158677017E-5 0.0 0.10911246602764756 7.159610631735403E-5 16 0.0 3.5798053158677017E-5 0.0 0.13170103757077276 7.159610631735403E-5 17 0.0 3.5798053158677017E-5 0.0 0.15339465778493103 7.159610631735403E-5 18 0.0 3.5798053158677017E-5 0.0 0.17698557481649918 7.159610631735403E-5 19 0.0 3.5798053158677017E-5 0.0 0.1998605307848938 7.159610631735403E-5 20 0.0 3.5798053158677017E-5 0.0 0.22409581277331814 7.159610631735403E-5 21 0.0 3.5798053158677017E-5 0.0 0.25294904361921183 7.159610631735403E-5 22 0.0 3.5798053158677017E-5 0.0 0.28638442526941615 7.159610631735403E-5 23 0.0 3.5798053158677017E-5 0.0 0.32017778745120723 7.159610631735403E-5 24 0.0 3.5798053158677017E-5 0.0 0.3546155145898545 7.159610631735403E-5 25 0.0 3.5798053158677017E-5 0.0 0.38905324172850186 7.159610631735403E-5 26 0.0 3.5798053158677017E-5 0.0 0.42517347736560696 7.159610631735403E-5 27 0.0 3.5798053158677017E-5 0.0 0.4617590876937749 7.159610631735403E-5 28 0.0 3.5798053158677017E-5 0.0 0.4974139486398172 7.159610631735403E-5 29 0.0 3.5798053158677017E-5 0.0 0.5375077681775354 1.0739415947603105E-4 30 0.0 7.159610631735403E-5 0.0 0.5801790475426785 1.0739415947603105E-4 31 0.0 1.0739415947603105E-4 0.0 0.6271818913400213 1.0739415947603105E-4 32 0.0 1.0739415947603105E-4 0.0 0.6781225209848187 1.0739415947603105E-4 33 0.0 1.0739415947603105E-4 0.0 0.7328935423175946 1.0739415947603105E-4 34 0.0 1.0739415947603105E-4 0.0 0.7912443689662382 1.0739415947603105E-4 35 0.0 1.0739415947603105E-4 0.0 0.8489866287111841 1.0739415947603105E-4 36 0.0 1.0739415947603105E-4 0.0 0.9076954358914144 1.0739415947603105E-4 37 0.0 1.0739415947603105E-4 0.0 0.9715949607796529 1.0739415947603105E-4 38 0.0 1.0739415947603105E-4 0.0 1.0330960161062601 1.0739415947603105E-4 39 0.0 1.0739415947603105E-4 0.0 1.0990002319713845 1.0739415947603105E-4 40 0.0 1.0739415947603105E-4 0.0 1.1684842531523765 1.0739415947603105E-4 41 0.0 1.0739415947603105E-4 0.0 1.2438391550513916 1.0739415947603105E-4 42 0.0 1.4319221263470807E-4 0.0 1.324277380498939 1.0739415947603105E-4 43 0.0 1.4319221263470807E-4 0.0 1.412161601003491 1.0739415947603105E-4 44 0.0 1.4319221263470807E-4 0.0 1.5058451061197489 1.0739415947603105E-4 45 0.0 1.4319221263470807E-4 0.0 1.6013901100002577 1.4319221263470807E-4 46 0.0 1.4319221263470807E-4 0.0 1.7090706539015583 1.4319221263470807E-4 47 0.0 1.4319221263470807E-4 0.0 1.8169301880686521 1.4319221263470807E-4 48 0.0 1.4319221263470807E-4 0.0 1.9272955859568532 1.789902657933851E-4 49 0.0 1.4319221263470807E-4 0.0 2.0409902027888114 1.789902657933851E-4 50 0.0 1.4319221263470807E-4 0.0 2.1570474911292425 1.789902657933851E-4 51 0.0 1.4319221263470807E-4 0.0 2.2825196674504054 1.789902657933851E-4 52 0.0 1.789902657933851E-4 0.0 2.4140417147553848 1.789902657933851E-4 53 0.0 1.789902657933851E-4 0.0 2.555873601370063 1.789902657933851E-4 54 0.0 1.789902657933851E-4 0.0 2.7090176727828834 1.789902657933851E-4 55 0.0 1.789902657933851E-4 0.0 2.86388005074732 1.789902657933851E-4 56 0.0 1.789902657933851E-4 0.0 3.0328826597094345 1.789902657933851E-4 57 0.0 1.789902657933851E-4 0.0 3.2018852686715484 1.789902657933851E-4 58 0.0 1.789902657933851E-4 0.0 3.3791572279133173 1.789902657933851E-4 59 0.0 2.147883189520621E-4 0.0 3.5593288294609384 1.789902657933851E-4 60 0.0 2.147883189520621E-4 0.0 3.7449059370355204 1.789902657933851E-4 61 0.0 2.147883189520621E-4 0.0 3.944193698969875 1.789902657933851E-4 62 0.0 2.147883189520621E-4 0.0 4.148135207814859 1.789902657933851E-4 63 0.0 2.147883189520621E-4 0.0 4.36618114960436 1.789902657933851E-4 64 0.0 2.147883189520621E-4 0.0 4.592818624151944 1.789902657933851E-4 65 0.0 2.147883189520621E-4 0.0 4.832092811464541 1.789902657933851E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAC 240 0.0 26.62103 4 CGATCGT 420 0.0 24.507374 1 TCGCGCT 410 0.0 24.241592 37 TAACGCG 150 2.1318556E-8 23.66992 52 AATTCGC 435 0.0 23.666105 55 ATTCGCG 420 0.0 23.665258 56 CGCGGAA 420 0.0 22.821297 59 TCGCGGA 440 0.0 22.590778 58 CTCGCGC 450 0.0 22.085596 36 CGCGCTT 465 0.0 21.376986 38 AACGCGA 185 8.469215E-9 21.110252 53 TATCGCG 85 0.0034832347 20.892336 66 CCTCGCG 500 0.0 20.585457 35 CGGTATA 140 7.3090177E-6 20.284508 10 CAATTCG 515 0.0 19.300858 54 CGCTCCG 565 0.0 18.847424 25 ATCGTTC 550 0.0 18.069386 3 AACTCGA 575 0.0 17.90232 13 ACGCGAA 220 6.194023E-8 17.749897 54 ACTCGAG 585 0.0 17.595984 14 >>END_MODULE