FastQCFastQC Report
Thu 26 May 2016
SRR936108_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936108_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793448
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86370.3091877851314934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62230.2227712848064471No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44010.15754723195133757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12450.047.814423100-101
GGTATCA85000.039.1388781
CGGTATC11750.036.477318100-101
TACGCAG11350.034.091473100-101
CGTACAT20300.033.7233100-101
TCGTATC5300.032.57245100-101
CCTGTCG8750.032.3113896-97
CGAGTAC13600.031.734186100-101
CCGTACA8450.031.701809100-101
GTATCAA169050.031.5433881
ACGTATC5400.029.213285100-101
ACCGTAT3600.028.93587398-99
ACCGTAC2450.027.9404198-99
CCGGTAT4850.027.616526100-101
GCGTACA5500.027.599787100-101
AGGTATC24450.027.390623100-101
TCGGTAT4400.027.058619100-101
TCGAGTA5950.027.01314100-101
AGTATCA33800.026.946537100-101
CGACGCA1550.026.884045100-101
Produced by FastQC (version 0.11.5)