FastQCFastQC Report
Thu 26 May 2016
SRR936107_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936107_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2416139
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115180.47671098392931865No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50030.20706590142371778No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCGT1200.001220857617.74803427
ATACGTA2803.252353E-916.4789521
TCGCGGG1350.002677560615.78158463
CGTAAAT1901.0060148E-414.94663924
TTGCGCG2151.7317161E-514.85820310
CGTCTTA1450.004319905314.68741910
TAGTCGG2453.6918318E-614.4890916
TTGCGAG2252.6370546E-514.21078369
TACGCTC1500.005405043714.19989654
TTACGGC2001.5572888E-414.19842758
GACGTAT2555.5944274E-613.91916139
ACGTTCG1800.001132802113.80431259
TCGTACC3353.44844E-813.77944349
CGTACCA3353.4493496E-813.77915850
TTAGTCG2606.829414E-613.65318115
CGATTGC1850.001384300113.44235468
CGTAGGC3455.1055395E-813.3731154
TACGTAG3455.1069946E-813.3728372
GTAGCCG4901.6370905E-1113.04072223
CGGTTAC2455.9301594E-513.0372124
Produced by FastQC (version 0.11.5)