##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936107_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2416139 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.678375292149994 40.0 36.0 41.0 30.0 41.0 2 36.54232268921614 39.0 36.0 41.0 30.0 41.0 3 36.44972288432081 39.0 36.0 41.0 30.0 41.0 4 36.33104552345705 39.0 36.0 41.0 29.0 41.0 5 36.25971974294525 39.0 36.0 41.0 29.0 41.0 6 36.153025136384954 39.0 35.0 41.0 29.0 41.0 7 35.980769732204976 39.0 35.0 40.0 29.0 41.0 8 35.79345683340238 39.0 35.0 40.0 28.0 41.0 9 35.698699040080065 39.0 35.0 40.0 27.0 41.0 10-11 35.90117497379083 39.0 35.0 40.5 29.0 41.0 12-13 36.06551920233066 39.0 35.0 41.0 29.0 41.0 14-15 36.00653315061758 39.0 35.0 41.0 29.0 41.0 16-17 35.83713395628314 39.0 35.0 41.0 27.5 41.0 18-19 35.67579038292085 39.0 35.0 41.0 27.0 41.0 20-21 35.44849985865879 39.0 35.0 41.0 26.5 41.0 22-23 35.157550952159625 39.0 35.0 41.0 25.0 41.0 24-25 34.91644396286803 39.0 34.5 41.0 24.0 41.0 26-27 33.98761660649491 38.0 33.5 40.0 23.0 40.5 28-29 34.02574727695716 38.0 33.5 40.0 23.0 40.5 30-31 34.40801522594519 38.0 34.0 40.0 23.0 41.0 32-33 34.266057954447156 38.0 34.0 40.0 22.5 41.0 34-35 34.009314654496286 38.0 33.5 40.0 21.5 41.0 36-37 33.72812532722662 38.0 33.0 40.0 20.0 41.0 38-39 33.457420909972484 37.0 33.0 40.0 19.5 41.0 40-41 33.08718103552817 37.0 32.5 40.0 18.0 41.0 42-43 32.73443932654537 36.0 32.0 39.5 15.0 41.0 44-45 32.370784545094466 36.0 32.0 39.0 12.5 41.0 46-47 31.986220784483013 35.5 31.5 39.0 9.0 40.0 48-49 31.51288729663318 35.0 31.0 38.0 7.0 40.0 50-51 31.0683412667897 35.0 31.0 37.0 3.5 39.0 52-53 30.606749446120443 35.0 30.5 37.0 2.0 39.0 54-55 30.14436793578515 35.0 30.0 36.0 2.0 39.0 56-57 29.682698305023013 34.0 29.5 36.0 2.0 37.5 58-59 29.250676182123627 34.0 29.0 35.0 2.0 37.0 60-61 28.892248541991997 34.0 29.0 35.0 2.0 36.5 62-63 28.552355431537674 34.0 29.0 35.0 2.0 36.0 64-65 28.18561659738947 34.0 29.0 35.0 2.0 36.0 66-67 27.80558527468825 34.0 27.5 35.0 2.0 35.5 68-69 27.45007220197182 34.0 27.0 35.0 2.0 35.0 70-71 27.037608142577888 33.5 26.0 35.0 2.0 35.0 72-73 26.566275574377137 33.0 25.0 35.0 2.0 35.0 74-75 26.000925857328575 33.0 24.5 35.0 2.0 35.0 76-77 24.325132163339937 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6570008505765443 1101 2 0.7247161390386907 1101 3 0.7823790997689244 1101 4 0.8468739705777111 1101 5 0.9179186472946768 1101 6 0.6602191110959836 1101 7 0.797897540111272 1101 8 1.0653724968637874 1101 9 0.8935601698869249 1101 10-11 0.8796458705359882 1101 12-13 0.9760452273263382 1101 14-15 0.9866048641128913 1101 16-17 0.9791656222244498 1101 18-19 0.9957071678577876 1101 20-21 0.9928646856161052 1101 22-23 1.0609002575873845 1101 24-25 1.0846420389751756 1101 26-27 0.9635532313523925 1101 28-29 0.9483132896297803 1101 30-31 1.0370290413874343 1101 32-33 1.1178590584739325 1101 34-35 1.0249725283157218 1101 36-37 1.0629209152783474 1101 38-39 1.050754925737138 1101 40-41 0.9629685981855047 1101 42-43 1.010112507620157 1101 44-45 0.9107534439652767 1101 46-47 0.8376668463545585 1101 48-49 1.028881465195294 1101 50-51 0.9345590964100268 1101 52-53 1.0727879133039586 1101 54-55 1.0690726738984928 1101 56-57 1.0603691172205707 1101 58-59 1.0812518665510424 1101 60-61 1.0097961682508263 1101 62-63 1.0291844557620244 1101 64-65 1.0044274611463635 1101 66-67 1.1084047563200308 1101 68-69 1.0917266836705615 1101 70-71 1.066708974365806 1101 72-73 1.1030536336553496 1101 74-75 1.235025226650567 1101 76-77 1.3499879375383692 1102 1 0.8327161189248855 1102 2 0.9974455335850934 1102 3 0.8034150850308563 1102 4 0.8207748048229035 1102 5 1.042782668851693 1102 6 1.0378428600599818 1102 7 0.9300457043279309 1102 8 0.9309315198601027 1102 9 0.8185927429308606 1102 10-11 0.783213871656308 1102 12-13 0.9811547446045807 1102 14-15 0.8389139691424745 1102 16-17 1.0055114598539419 1102 18-19 0.992875132183265 1102 20-21 0.8979924630201452 1102 22-23 0.9832075411808674 1102 24-25 0.8828088413342527 1102 26-27 0.7855369125662932 1102 28-29 0.856606841974795 1102 30-31 0.889367092606399 1102 32-33 0.9007523410816347 1102 34-35 0.9933640903985435 1102 36-37 1.0328945639489504 1102 38-39 0.9881672004774344 1102 40-41 0.908909472905016 1102 42-43 0.9030269381078782 1102 44-45 0.9597471005165659 1102 46-47 0.7730129581147693 1102 48-49 0.9060273160050336 1102 50-51 0.9464353861172157 1102 52-53 0.8356178254951807 1102 54-55 0.8686798066056767 1102 56-57 0.893437816024143 1102 58-59 0.8508337920469842 1102 60-61 0.8810344793676741 1102 62-63 0.8152679974392747 1102 64-65 0.8665429173131329 1102 66-67 0.8450264682960622 1102 68-69 0.5577993470723719 1102 70-71 0.750518192205444 1102 72-73 0.8563300448221298 1102 74-75 0.8848012769624738 1102 76-77 0.8804821187822434 1103 1 0.4744947627471632 1103 2 0.5083173722722663 1103 3 0.4990655502213883 1103 4 0.5836681829130583 1103 5 0.5446340165964401 1103 6 0.5542050172509079 1103 7 0.6857721595942792 1103 8 0.5404825469384562 1103 9 0.6685667423526738 1103 10-11 0.5513146926173462 1103 12-13 0.5742533562936032 1103 14-15 0.6946862529257984 1103 16-17 0.7591893981746196 1103 18-19 0.8054694703186769 1103 20-21 0.8214025531984106 1103 22-23 0.7657367957169825 1103 24-25 0.7139255384126386 1103 26-27 0.5245742605872508 1103 28-29 0.42028116996753084 1103 30-31 0.768057187474092 1103 32-33 0.7237448011360001 1103 34-35 0.6554753639916839 1103 36-37 0.7094229472103493 1103 38-39 0.6691659734988278 1103 40-41 0.7314095650505337 1103 42-43 0.7627078570584658 1103 44-45 0.578237574890565 1103 46-47 0.4120538809376768 1103 48-49 0.41603776064378195 1103 50-51 0.42037319255657124 1103 52-53 0.4066840001862033 1103 54-55 0.4188061902871709 1103 56-57 0.4383434345914452 1103 58-59 0.3389297015409909 1103 60-61 0.4535232476063804 1103 62-63 0.40677571124018996 1103 64-65 0.36176644901318866 1103 66-67 0.4091734028700813 1103 68-69 0.291521897145131 1103 70-71 0.3076046708834177 1103 72-73 0.47584433359418554 1103 74-75 0.3663778356760439 1103 76-77 0.6678717717511269 1104 1 0.739604803583056 1104 2 0.7612112512771461 1104 3 0.732417199778844 1104 4 0.6008314133885548 1104 5 0.8253930341715758 1104 6 0.5623608013794339 1104 7 0.7712966872973581 1104 8 0.8242190060156176 1104 9 0.8574956399523188 1104 10-11 0.6989839741993222 1104 12-13 0.7585274429723938 1104 14-15 0.8340906548968618 1104 16-17 0.7853168166336602 1104 18-19 0.8398957420301585 1104 20-21 0.8250484946776524 1104 22-23 0.7687625599762029 1104 24-25 0.799808582991119 1104 26-27 0.761728214781499 1104 28-29 0.8061989478465961 1104 30-31 0.8398451801340983 1104 32-33 0.8420247726403431 1104 34-35 0.8577278375260136 1104 36-37 0.7910519010247654 1104 38-39 0.7563429706847131 1104 40-41 0.8239029095930661 1104 42-43 0.7330980348355638 1104 44-45 0.6517451402507888 1104 46-47 0.48206131715586054 1104 48-49 0.7093039316751124 1104 50-51 0.7518793348221458 1104 52-53 0.700867881571348 1104 54-55 0.6169149346382596 1104 56-57 0.5577236456416124 1104 58-59 0.594849133788685 1104 60-61 0.4894354968555845 1104 62-63 0.514433749868008 1104 64-65 0.5295728152937826 1104 66-67 0.5177782721158728 1104 68-69 0.48808030395437996 1104 70-71 0.3995086466013369 1104 72-73 0.36483071206308537 1104 74-75 0.421342756308551 1104 76-77 0.5276214050046129 1105 1 0.3755360318961536 1105 2 0.47845857054954166 1105 3 0.3147227491528568 1105 4 0.5439909218325312 1105 5 0.39015001725064025 1105 6 0.18053658787730598 1105 7 0.2748197526815588 1105 8 0.35477682813564115 1105 9 0.23279832933408073 1105 10-11 0.37795973432262997 1105 12-13 0.4004813451722029 1105 14-15 0.15856893296723484 1105 16-17 0.20941019084757784 1105 18-19 0.3957791252279108 1105 20-21 0.568644007704215 1105 22-23 0.5526446079614331 1105 24-25 0.5398264270350026 1105 26-27 0.3311244432182292 1105 28-29 0.4617520307863714 1105 30-31 0.5647780074054012 1105 32-33 0.6436379483856385 1105 34-35 0.6822866816605568 1105 36-37 0.6669053655715729 1105 38-39 0.5856927139447734 1105 40-41 0.832993275457774 1105 42-43 0.6780543107465107 1105 44-45 0.4952413365435149 1105 46-47 0.4199996913285631 1105 48-49 0.5076149290458964 1105 50-51 0.6747236259644112 1105 52-53 0.5335171245388146 1105 54-55 0.3930552226155797 1105 56-57 0.4870486627438737 1105 58-59 0.5233897135346659 1105 60-61 0.2884112157686225 1105 62-63 0.0943404498325684 1105 64-65 0.2588389017199475 1105 66-67 0.3268777042384521 1105 68-69 0.2641272506859913 1105 70-71 0.12671277751810095 1105 72-73 0.01693902911836176 1105 74-75 -0.013848953834941113 1105 76-77 -0.13992139185351604 1106 1 0.3708725154067949 1106 2 0.5231046663266525 1106 3 0.20924953758730425 1106 4 0.4117055574799764 1106 5 0.11446152085030548 1106 6 0.420403029083424 1106 7 0.6209952724914842 1106 8 0.5962497779815763 1106 9 0.4108531391477541 1106 10-11 0.24580533201458366 1106 12-13 0.2752858222134549 1106 14-15 0.4199194879968502 1106 16-17 0.6745004738932607 1106 18-19 0.6721544311638326 1106 20-21 0.5705743943745105 1106 22-23 0.5480036852362034 1106 24-25 0.6801413697940575 1106 26-27 0.5685423746926688 1106 28-29 0.6573747701320372 1106 30-31 0.40390580632294615 1106 32-33 0.49392287517570566 1106 34-35 0.5769091613312725 1106 36-37 0.6039534136134819 1106 38-39 0.5756306168754151 1106 40-41 0.6294682393179798 1106 42-43 0.596551242697366 1106 44-45 0.42126137854133106 1106 46-47 0.6819304255952261 1106 48-49 0.6622941896061825 1106 50-51 0.5826120189037063 1106 52-53 0.5454754620467135 1106 54-55 0.7930846660728932 1106 56-57 0.5637833219869712 1106 58-59 0.4687362159995345 1106 60-61 0.43064386815384736 1106 62-63 0.28519079323733365 1106 64-65 0.28911147861541764 1106 66-67 0.21364573273317333 1106 68-69 0.13113381207866937 1106 70-71 0.2768909717753978 1106 72-73 0.37260004495853494 1106 74-75 0.1187105004796507 1106 76-77 0.02400372770941317 1107 1 0.30580151248231147 1107 2 0.1713390040753282 1107 3 0.25681730155906735 1107 4 0.2989374634160171 1107 5 0.2919653679486416 1107 6 0.06636451884001815 1107 7 -0.11147685480955971 1107 8 0.2033557669841457 1107 9 0.20112180864062879 1107 10-11 0.2763609923490975 1107 12-13 0.31928294391416756 1107 14-15 0.3763984904225097 1107 16-17 0.4182094384984154 1107 18-19 0.409813790332322 1107 20-21 0.3071489064531292 1107 22-23 0.31374484152885174 1107 24-25 0.21498849815358767 1107 26-27 0.3073470106097531 1107 28-29 0.398295310134543 1107 30-31 0.4815291463356175 1107 32-33 0.45381894386074606 1107 34-35 0.60851291058745 1107 36-37 0.5783692474157576 1107 38-39 0.5804536349558163 1107 40-41 0.6447728201940279 1107 42-43 0.5601457412120325 1107 44-45 0.5363351335171558 1107 46-47 0.6152828629119611 1107 48-49 0.7940456643592846 1107 50-51 0.632654897018238 1107 52-53 0.6735660349429757 1107 54-55 0.7453538959834987 1107 56-57 0.5986052392468366 1107 58-59 0.6125648659701639 1107 60-61 0.5181810400920099 1107 62-63 0.5888818714333119 1107 64-65 0.5934589114326485 1107 66-67 0.7283733450842256 1107 68-69 0.481134889805066 1107 70-71 0.490571303499582 1107 72-73 0.5191026111020705 1107 74-75 0.39790033505634526 1107 76-77 0.4666583872210026 1108 1 -0.03146182473637538 1108 2 -0.09286793576148966 1108 3 0.07233456148912865 1108 4 0.021838028286452982 1108 5 -0.03318696774579166 1108 6 -0.12373966837418493 1108 7 -0.1960209236699555 1108 8 0.019989497818897917 1108 9 -0.013963280573037196 1108 10-11 -0.10863945596076974 1108 12-13 -0.07706521353225781 1108 14-15 0.05953011420086085 1108 16-17 -0.09544715613980514 1108 18-19 -0.2717912486706382 1108 20-21 -0.22274251289592684 1108 22-23 -0.14624798551320595 1108 24-25 0.08800885142176185 1108 26-27 -0.12094986018734488 1108 28-29 -0.07794408733698788 1108 30-31 0.12117381593124321 1108 32-33 0.19497683859759007 1108 34-35 0.19322357139620294 1108 36-37 0.02261196238033847 1108 38-39 -0.05018439965370192 1108 40-41 0.14265652861329414 1108 42-43 0.151466551756279 1108 44-45 0.022201088865493546 1108 46-47 0.05658887923044631 1108 48-49 -0.016475092387775447 1108 50-51 -0.018482375464500933 1108 52-53 -0.04332117029328586 1108 54-55 -0.01933058352874184 1108 56-57 -0.0488422636671757 1108 58-59 -6.736348816218651E-4 1108 60-61 -0.06512676946595874 1108 62-63 -0.08572289858372173 1108 64-65 -0.15668669961370796 1108 66-67 -0.20387152176774137 1108 68-69 -0.33473438303632363 1108 70-71 -0.4082603113125707 1108 72-73 -0.20925863699261527 1108 74-75 -0.23217569829790818 1108 76-77 -0.2680704749685425 1109 1 -0.16382127297529792 1109 2 -0.06979307303214455 1109 3 -0.11657611214151586 1109 4 0.0166186184386774 1109 5 -0.09650521246700805 1109 6 -0.03871471413106775 1109 7 -0.2264820637976257 1109 8 -0.07847360650680457 1109 9 0.024461254045952785 1109 10-11 -0.1709148008114454 1109 12-13 6.314071543229716E-4 1109 14-15 0.10040936066307182 1109 16-17 0.11649668565297588 1109 18-19 0.006967378176376826 1109 20-21 0.0770194620159046 1109 22-23 0.001752103768531299 1109 24-25 -0.05248184095803765 1109 26-27 -0.09210561216270463 1109 28-29 0.001495044844602944 1109 30-31 0.06520113614016765 1109 32-33 -0.010345602364871809 1109 34-35 -0.02914489429728917 1109 36-37 0.015396302365161318 1109 38-39 -0.03673848206074126 1109 40-41 0.10139390640892287 1109 42-43 0.03708585446597823 1109 44-45 -0.025356877296729863 1109 46-47 -0.09800966547738099 1109 48-49 0.16956999488511215 1109 50-51 0.10089524160616392 1109 52-53 0.11750673917503818 1109 54-55 0.10400283908631991 1109 56-57 0.008951763385184108 1109 58-59 0.1608887060852986 1109 60-61 0.1187910913438266 1109 62-63 0.09647048564019656 1109 64-65 0.05598273478654647 1109 66-67 0.25595768228565063 1109 68-69 0.1650258955226711 1109 70-71 0.18827100425369636 1109 72-73 0.046062375462184946 1109 74-75 0.09781385573985801 1109 76-77 0.3044397701097985 1110 1 -0.1997344216414092 1110 2 -0.2504758636739908 1110 3 -0.4616961313470682 1110 4 -0.12052450676202398 1110 5 -0.040995704716316084 1110 6 -0.22026534205411252 1110 7 -0.3530072895439744 1110 8 -0.1484724785942788 1110 9 -0.22595343489336273 1110 10-11 -0.2088594854978112 1110 12-13 -0.11142937525824692 1110 14-15 -0.062208375985562725 1110 16-17 -0.28802043154058055 1110 18-19 -0.08537588941035068 1110 20-21 -0.24344566565378756 1110 22-23 -0.33122690291651224 1110 24-25 -0.17655894464424904 1110 26-27 -0.06286163433601644 1110 28-29 0.00863659372720349 1110 30-31 -0.005919439778182323 1110 32-33 0.09727156687823424 1110 34-35 0.2052075614649027 1110 36-37 0.21857206597400847 1110 38-39 0.26800425213029655 1110 40-41 0.3247480078196432 1110 42-43 0.20310431635892456 1110 44-45 0.21298647445227914 1110 46-47 0.224259691070948 1110 48-49 0.26293493736329765 1110 50-51 0.131019732795167 1110 52-53 0.2207865328808758 1110 54-55 0.2460475547816543 1110 56-57 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-1.2351467335504793 2314 18-19 -1.0382713241080737 2314 20-21 -0.7953634747231888 2314 22-23 -0.6951845272657309 2314 24-25 -0.7279083071016146 2314 26-27 -0.8036544318354473 2314 28-29 -0.8082490501421447 2314 30-31 -0.9054371574211828 2314 32-33 -0.9677139028545767 2314 34-35 -1.0375942960993427 2314 36-37 -1.1347852366557376 2314 38-39 -1.0988357460023224 2314 40-41 -0.9315861983101925 2314 42-43 -0.8450596941837176 2314 44-45 -0.8197319254885684 2314 46-47 -0.7764410512693303 2314 48-49 -0.8260630503383943 2314 50-51 -0.7467827980538218 2314 52-53 -0.6666160398493908 2314 54-55 -0.6105736946243283 2314 56-57 -0.4284793257982855 2314 58-59 -0.5459914849148007 2314 60-61 -0.5392562005419883 2314 62-63 -0.49564459732294 2314 64-65 -0.4405038336897711 2314 66-67 -0.45466529119462606 2314 68-69 -0.3144329825543615 2314 70-71 -0.2919056156816602 2314 72-73 -0.06157526465999297 2314 74-75 -0.08538465300052067 2314 76-77 -0.06597792441498385 2315 1 -1.4501610088124082 2315 2 -1.4451856977986068 2315 3 -1.3711213696504743 2315 4 -1.252936471848038 2315 5 -1.2432986865658364 2315 6 -1.1976899956403884 2315 7 -1.2872860541281241 2315 8 -1.2165760899153426 2315 9 -1.39802640260298 2315 10-11 -1.43922674189281 2315 12-13 -1.561679577380545 2315 14-15 -1.7504754448415483 2315 16-17 -1.517736047021181 2315 18-19 -1.3806035861583084 2315 20-21 -1.4760282656081998 2315 22-23 -1.423393148249687 2315 24-25 -1.6083505137714553 2315 26-27 -1.6167160217582293 2315 28-29 -1.4678886762368393 2315 30-31 -1.699774090461652 2315 32-33 -1.7580209198530525 2315 34-35 -1.6580117912864836 2315 36-37 -1.9125592023287084 2315 38-39 -1.914691169132766 2315 40-41 -1.9005849310836531 2315 42-43 -1.92524608161591 2315 44-45 -1.9350021748303696 2315 46-47 -1.7771362171561762 2315 48-49 -1.73857784501039 2315 50-51 -1.556357028024351 2315 52-53 -1.668384561309157 2315 54-55 -1.796809647457728 2315 56-57 -1.7960284915879186 2315 58-59 -1.6582613691235295 2315 60-61 -1.7651239002827772 2315 62-63 -1.9777705987540806 2315 64-65 -1.8795393849898367 2315 66-67 -1.9709878133484828 2315 68-69 -1.9644358086617153 2315 70-71 -2.1715666862789504 2315 72-73 -3.311689552441372 2315 74-75 -3.96266184552287 2315 76-77 -3.6323620557091516 2316 1 -3.1860355385706853 2316 2 -3.10392747955531 2316 3 -2.353889986748257 2316 4 -2.4236285462173512 2316 5 -3.1142755428420656 2316 6 -2.6616000416337897 2316 7 -2.958785809618412 2316 8 -2.7281831100610248 2316 9 -2.58095291225073 2316 10-11 -2.4864867947580507 2316 12-13 -2.543890137004162 2316 14-15 -2.656341121110678 2316 16-17 -3.8857890145381404 2316 18-19 -3.5904696331872756 2316 20-21 -2.986173341374247 2316 22-23 -3.109771240178951 2316 24-25 -3.1599583649305636 2316 26-27 -3.1283281558326514 2316 28-29 -3.240190359678259 2316 30-31 -3.184206652124452 2316 32-33 -3.3025600430060464 2316 34-35 -3.26770780018472 2316 36-37 -3.2366924713117697 2316 38-39 -3.2612336738711427 2316 40-41 -3.2305979396398925 2316 42-43 -3.4858183302423384 2316 44-45 -3.4198342621762734 2316 46-47 -3.080877695273358 2316 48-49 -3.1670732952119103 2316 50-51 -3.5890110611562136 2316 52-53 -4.588180259855843 2316 54-55 -3.6336305736541483 2316 56-57 -3.5700145753093224 2316 58-59 -3.565656749328152 2316 60-61 -3.422696829700971 2316 62-63 -3.317782936107175 2316 64-65 -3.2355436167658738 2316 66-67 -3.3599228696496404 2316 68-69 -3.324063898193824 2316 70-71 -3.0524396339155153 2316 72-73 -3.020970693870268 2316 74-75 -3.1026415815802224 2316 76-77 -3.261226987262411 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56836.0 3 5223.0 4 6966.0 5 8384.0 6 9428.0 7 9925.0 8 12002.0 9 12877.0 10 16440.0 11 18678.0 12 14176.0 13 11232.0 14 11068.0 15 11186.0 16 11862.0 17 12514.0 18 12924.0 19 13676.0 20 14467.0 21 15696.0 22 17515.0 23 19788.0 24 23020.0 25 26978.0 26 31755.0 27 37523.0 28 44199.0 29 52307.0 30 61761.0 31 74493.0 32 90954.0 33 114500.0 34 152313.0 35 202511.0 36 286461.0 37 402568.0 38 385735.0 39 105390.0 40 808.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.125626420531567 26.937107316542136 29.372546730857824 21.56471953206847 2 21.806066195493525 27.09169662896233 29.377923962689177 21.724313212854963 3 22.04162004663452 27.258137756865512 28.926482951659633 21.77375924484034 4 21.645501712486123 27.176786354420468 29.35003348806558 21.82767844502783 5 21.995368925440033 27.34726519069447 29.169560743323064 21.487805140542434 6 22.037300873124217 27.163749491912405 29.290059861204128 21.50888977375925 7 22.02271255871868 26.909629946070712 29.206053922664378 21.861603572546233 8 21.79015113036272 27.34300908752596 28.931515083024628 21.935324699086696 9 21.84038702984728 26.929364237988416 29.18255385222941 22.04769487993489 10-11 22.131308376975706 27.21793698360322 28.993333904606487 21.657420734814586 12-13 22.27871701579442 26.902771945126137 28.845660690006884 21.97285034907256 14-15 21.97769607513424 27.236699548933803 29.02277268602212 21.762831689909838 16-17 22.20286041097583 27.109168675339607 29.011232081040355 21.676738832644208 18-19 22.123992715198753 26.93234237737945 28.82714410167261 22.11652080574919 20-21 22.112196738508782 27.201945394250547 28.70034027426035 21.98551759298032 22-23 22.20243895775084 27.242900586267222 28.29240253648957 22.26225791949237 24-25 22.14745138786117 27.53937559959887 28.044927354748307 22.268245657791656 26-27 22.24917067443533 27.540522380923004 27.936565960827437 22.273740983814232 28-29 22.412256610996064 27.5068224047233 27.920098909863956 22.160822074416675 30-31 22.191056464681374 27.41309265356891 28.015178388491414 22.380672493258306 32-33 22.24764191213374 27.705284289934855 27.82915181273962 22.217921985191776 34-35 22.31888917174251 27.338250847008617 27.986282289293023 22.356577691955852 36-37 22.326408679969585 27.48367973157396 28.12688726710684 22.063024321349616 38-39 22.256045755320223 27.4104113884949 28.219520065038324 22.11402279114656 40-41 22.09602184226201 27.3333134646899 28.195364231557452 22.375300461490635 42-43 22.061384500053606 27.704489092383483 27.85719282602909 22.376933581533816 44-45 22.20565769885034 27.398646124450003 28.013368625909663 22.382327550789995 46-47 22.01286671482715 27.377765174174666 28.13948048775944 22.469887623238744 48-49 22.172675323471008 27.37342306006918 27.95736717398248 22.496534442477333 50-51 21.99166576785257 27.511039886850863 27.90350939117542 22.593784954121148 52-53 21.90466272800881 27.365073502543662 28.156876644457284 22.573387124990244 54-55 21.9112595911674 27.37892105305103 28.01739725519818 22.692422100583393 56-57 21.857554315709514 27.533109995456655 27.91924234850437 22.690093340329458 58-59 22.058593420131043 27.48556131226477 27.830715493274916 22.625129774329274 60-61 21.920790779919447 27.306505251919727 27.803868166641514 22.968835801519308 62-63 21.8916678310279 27.216245167900887 27.98929822654847 22.90278877452274 64-65 21.91764318920936 27.122876967784947 28.033102028709152 22.926377814296533 66-67 21.761887984936926 27.21297915123644 28.182801250122647 22.842331613703987 68-69 21.947506566026924 27.08236114452381 28.101932728606016 22.86819956084325 70-71 21.67973255071925 26.924610725551005 28.17334095849244 23.22231576523731 72-73 21.81213683548168 26.774744896796758 28.156401161049416 23.256717106672138 74-75 21.714740320249838 26.76939704140995 28.04705757591637 23.468805062423844 76-77 21.4812633005772 26.668845713925894 28.4492847463792 23.40060623911771 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13005.0 1 7657.5 2 2095.0 3 1843.0 4 1806.0 5 1811.5 6 1869.5 7 2049.0 8 2183.5 9 2191.0 10 2236.5 11 2333.5 12 2543.0 13 2701.0 14 2794.5 15 3142.5 16 3522.5 17 3648.0 18 3988.5 19 4754.0 20 5711.0 21 7213.5 22 8184.0 23 9336.5 24 11921.0 25 15667.0 26 17981.0 27 20855.0 28 27542.0 29 34740.5 30 38126.0 31 43462.0 32 53738.5 33 63739.0 34 73175.0 35 77551.0 36 83793.0 37 94026.0 38 99090.5 39 100164.0 40 102388.5 41 105835.0 42 110800.0 43 114543.0 44 114457.5 45 114459.0 46 115616.5 47 118322.5 48 119958.0 49 118417.5 50 115094.5 51 108579.0 52 103846.0 53 100801.5 54 92326.0 55 80243.0 56 68394.0 57 63197.0 58 59572.0 59 49893.5 60 40105.5 61 36371.0 62 31285.5 63 21858.5 64 14938.0 65 12359.0 66 10634.0 67 7609.5 68 5426.5 69 3758.0 70 2973.0 71 2488.5 72 1816.0 73 1551.0 74 1474.0 75 1312.0 76 905.0 77 506.0 78 352.0 79 291.0 80 214.5 81 162.5 82 111.5 83 97.0 84 89.0 85 75.0 86 63.5 87 58.0 88 56.5 89 46.5 90 34.5 91 31.0 92 29.5 93 23.0 94 18.5 95 18.5 96 18.0 97 25.5 98 34.5 99 66.0 100 96.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009519319873566877 2 0.01345121286482276 3 0.013740931295757404 4 0.014610086588561338 5 0.011795678973767652 6 0.008981271358973966 7 0.01585173700685267 8 0.01696922238331487 9 0.016886445688762113 10-11 0.01994918338721406 12-13 0.015748266138661725 14-15 0.008132810239808223 16-17 0.006518664696029491 18-19 0.017569353418822344 20-21 0.024315654024871913 22-23 0.021397775541887286 24-25 0.01910072226804832 26-27 0.025743552005906944 28-29 0.008360446149828301 30-31 0.008443222844381057 32-33 0.010409169340008998 34-35 0.011754290626491274 36-37 0.011505960542833009 38-39 0.013761625469395595 40-41 0.011257630459174741 42-43 0.014485921546732205 44-45 0.01380301381667197 46-47 0.024998561754932147 48-49 0.024108712288490024 50-51 0.03805658532062932 52-53 0.04215403170099071 54-55 0.028992537267102596 56-57 0.021377081368249094 58-59 0.016845057341485736 60-61 0.020632091117274296 62-63 0.026964508250560088 64-65 0.0263850713886908 66-67 0.028806289704358893 68-69 0.04370609472385488 70-71 0.05488094848847687 72-73 0.08757774283681527 74-75 0.08763982535772982 76-77 0.09270989789908611 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2416139.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.45588725311483 #Duplication Level Percentage of deduplicated Percentage of total 1 74.42067043888537 39.782229682822724 2 13.323573420484053 14.244468771479859 3 5.081239097114463 8.148664328444093 4 2.4272818342429114 5.1901001625129135 5 1.3311804827895835 3.5579716900773475 6 0.8030455305575909 2.575650680436262 7 0.5196049469479076 1.9443160422145647 8 0.38343715943996476 1.63975788509419 9 0.27317433702826527 1.3142498904564606 >10 1.2879158029187414 12.399674301372293 >50 0.10066608441091185 3.744275729207892 >100 0.0468112542471422 4.3783185063008965 >500 0.0012440986072367027 0.39362288719711375 >1k 0.0 0.0 >5k 7.775616295215906E-5 0.20795092974067214 >10k+ 7.775616295215906E-5 0.4787485126430265 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11518 0.47671098392931865 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5003 0.20706590142371778 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.655533891055109E-4 0.0 2 0.0 0.0 0.0 4.966601673165327E-4 0.0 3 0.0 0.0 0.0 6.622135564220436E-4 0.0 4 0.0 0.0 0.0 0.0012830387655677095 0.0 5 0.0 0.0 0.0 0.0028144076147936853 0.0 6 0.0 0.0 0.0 0.005132155062270838 0.0 7 0.0 0.0 0.0 0.010843746986410965 0.0 8 0.0 0.0 0.0 0.015934513701405424 0.0 9 0.0 0.0 0.0 0.021480552236440038 0.0 10 0.0 0.0 0.0 0.029220173177122673 0.0 11 4.138834727637772E-5 0.0 0.0 0.03803589114699113 0.0 12 4.138834727637772E-5 0.0 0.0 0.050700725413562714 0.0 13 4.138834727637772E-5 0.0 0.0 0.06849771474240514 0.0 14 4.138834727637772E-5 0.0 0.0 0.08811579135140818 0.0 15 4.138834727637772E-5 0.0 0.0 0.11116910078435056 0.0 16 4.138834727637772E-5 0.0 0.0 0.13269104136806698 0.0 17 4.138834727637772E-5 0.0 0.0 0.15470964211909993 0.0 18 8.277669455275545E-5 0.0 0.0 0.180577359166836 0.0 19 8.277669455275545E-5 0.0 0.0 0.20561730926904453 0.0 20 8.277669455275545E-5 0.0 0.0 0.23123669623312235 0.0 21 8.277669455275545E-5 0.0 0.0 0.2621951799958529 0.0 22 8.277669455275545E-5 0.0 0.0 0.29365032392589996 0.0 23 1.2416504182913318E-4 0.0 0.0 0.32696794348338404 0.0 24 1.2416504182913318E-4 0.0 0.0 0.360823611555461 0.0 25 1.2416504182913318E-4 0.0 0.0 0.39621064847676396 0.0 26 1.2416504182913318E-4 0.0 0.0 0.4333359959836748 0.0 27 1.2416504182913318E-4 0.0 0.0 0.4708752269633494 0.0 28 1.2416504182913318E-4 0.0 0.0 0.5094491666249334 0.0 29 1.2416504182913318E-4 0.0 0.0 0.5473195043828191 0.0 30 1.2416504182913318E-4 0.0 0.0 0.592018919441307 0.0 31 1.2416504182913318E-4 0.0 0.0 0.6395327421145887 0.0 32 1.655533891055109E-4 0.0 0.0 0.6900679141390458 0.0 33 1.655533891055109E-4 0.0 0.0 0.744328037418377 0.0 34 1.655533891055109E-4 0.0 0.0 0.8019406168270948 0.0 35 1.655533891055109E-4 0.0 0.0 0.8635678659216213 0.0 36 1.655533891055109E-4 0.0 0.0 0.9273473090745193 0.0 37 1.655533891055109E-4 0.0 0.0 0.9915406357001811 0.0 38 1.655533891055109E-4 0.0 0.0 1.0602039038316917 0.0 39 1.655533891055109E-4 0.0 0.0 1.1281221817122276 0.0 40 1.655533891055109E-4 0.0 0.0 1.1957921295091052 0.0 41 1.655533891055109E-4 0.0 0.0 1.2729813971795496 0.0 42 1.655533891055109E-4 0.0 0.0 1.353978392799421 0.0 43 1.655533891055109E-4 0.0 0.0 1.446150242183914 0.0 44 2.0694173638188864E-4 0.0 0.0 1.5431645281997435 0.0 45 2.0694173638188864E-4 0.0 0.0 1.6457248527506074 0.0 46 2.0694173638188864E-4 0.0 0.0 1.7578872738695912 0.0 47 2.0694173638188864E-4 0.0 0.0 1.8698013649049166 0.0 48 2.0694173638188864E-4 0.0 0.0 1.9834537665258498 0.0 49 2.0694173638188864E-4 0.0 0.0 2.098140876828692 0.0 50 2.0694173638188864E-4 0.0 0.0 2.2173807053319368 0.0 51 2.0694173638188864E-4 0.0 0.0 2.3370344173079447 0.0 52 2.0694173638188864E-4 0.0 0.0 2.468773526688655 0.0 53 2.0694173638188864E-4 0.0 0.0 2.612101373306751 0.0 54 2.0694173638188864E-4 0.0 0.0 2.7630446758237004 0.0 55 2.0694173638188864E-4 0.0 0.0 2.92930166683291 0.0 56 2.0694173638188864E-4 0.0 0.0 3.1035052205191835 0.0 57 2.0694173638188864E-4 0.0 0.0 3.2813509487657786 0.0 58 2.0694173638188864E-4 0.0 0.0 3.467764064898584 0.0 59 2.0694173638188864E-4 0.0 0.0 3.650328064734686 0.0 60 2.0694173638188864E-4 0.0 0.0 3.8381069963276118 0.0 61 2.0694173638188864E-4 0.0 0.0 4.034287762417643 0.0 62 2.0694173638188864E-4 0.0 0.0 4.238166761101079 0.0 63 2.0694173638188864E-4 0.0 0.0 4.445687934344837 0.0 64 2.0694173638188864E-4 0.0 0.0 4.670881931875608 0.0 65 2.0694173638188864E-4 0.0 0.0 4.9074991132546595 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGCGT 120 0.0012208576 17.748034 27 ATACGTA 280 3.252353E-9 16.478952 1 TCGCGGG 135 0.0026775606 15.781584 63 CGTAAAT 190 1.0060148E-4 14.946639 24 TTGCGCG 215 1.7317161E-5 14.858203 10 CGTCTTA 145 0.0043199053 14.687419 10 TAGTCGG 245 3.6918318E-6 14.48909 16 TTGCGAG 225 2.6370546E-5 14.210783 69 TACGCTC 150 0.0054050437 14.199896 54 TTACGGC 200 1.5572888E-4 14.198427 58 GACGTAT 255 5.5944274E-6 13.919161 39 ACGTTCG 180 0.0011328021 13.804312 59 TCGTACC 335 3.44844E-8 13.779443 49 CGTACCA 335 3.4493496E-8 13.779158 50 TTAGTCG 260 6.829414E-6 13.653181 15 CGATTGC 185 0.0013843001 13.442354 68 CGTAGGC 345 5.1055395E-8 13.373115 4 TACGTAG 345 5.1069946E-8 13.372837 2 GTAGCCG 490 1.6370905E-11 13.040722 23 CGGTTAC 245 5.9301594E-5 13.037212 4 >>END_MODULE