FastQCFastQC Report
Thu 26 May 2016
SRR936107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936107_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2416139
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90420.37423343607300735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65030.26914842233828434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49560.205120649101728No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12500.052.146812100-101
CGTACAT18300.039.848198100-101
ACGTATC4350.039.685555100-101
CGGTATC10250.038.9112100-101
CGAGTAC10500.038.835148100-101
TACGCAG9250.038.61297100-101
TCGTATC5350.035.60573100-101
GGTATCA92550.034.2070051
CCGTATC5750.034.16408100-101
GCGTATC3150.034.016186100-101
CCGTACA7750.031.492407100-101
CGACGCA1800.031.417728100-101
TCGAGTA4850.031.298399100-101
CCTGTCG7100.031.01377796-97
AGTATCA33000.030.936691100-101
ACCGTAT3300.030.6645298-99
CCGGTAT4850.030.07101100-101
AGCGTAC2300.029.76262398-99
CTACGCG1700.029.7564696-97
AGGTATC22800.029.111443100-101
Produced by FastQC (version 0.11.5)