FastQCFastQC Report
Thu 26 May 2016
SRR936106_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936106_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418016
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114880.4751002474756164No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49510.20475464182205574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA1553.0235242E-822.90249810
TCGCGCT1557.653816E-720.6096937
TCGCGGA1601.0351741E-619.97142258
AACTCGA2052.7645001E-819.04541813
CGCGGAA1501.3230161E-518.93704259
CCTCGCG2206.209666E-817.74576635
ACGTAAT3750.017.03558232
ATACGTA2551.834269E-816.7025871
AACGCTC3206.366463E-1116.63715
GACGTAA3850.016.59344331
CTCGCGC2159.49507E-716.5080336
TAATTCG3900.016.38070735
GACGAAC2854.107278E-916.19179738
AGTACGC1806.3433705E-515.77662538
CGCGCTT2051.1024902E-515.58422738
ACGAGAC4600.015.4307827
ACGTATG1400.003418907515.21317438
CGATCGT2101.3867708E-515.2112861
CGCCGTA1450.004302228314.696485567
CGTAACG1450.004324764514.68493528
Produced by FastQC (version 0.11.5)