##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936106_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2418016 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.63316330413033 40.0 36.0 41.0 30.0 41.0 2 36.50640980043143 39.0 36.0 41.0 30.0 41.0 3 36.37034246258089 39.0 36.0 41.0 29.0 41.0 4 36.26593413773937 39.0 36.0 41.0 29.0 41.0 5 36.19897097455104 39.0 36.0 41.0 29.0 41.0 6 36.09926030266136 39.0 35.0 41.0 29.0 41.0 7 35.950532585392324 39.0 35.0 40.0 29.0 41.0 8 35.80709929131982 39.0 35.0 40.0 28.0 41.0 9 35.966820732369015 39.0 35.0 41.0 29.0 41.0 10-11 36.168600000992555 39.0 35.0 41.0 30.0 41.0 12-13 36.08041840914204 39.0 35.0 41.0 29.0 41.0 14-15 35.92645168601035 39.0 35.0 41.0 28.5 41.0 16-17 35.7751578153329 39.0 35.0 41.0 27.0 41.0 18-19 35.60867835448566 39.0 35.0 41.0 27.0 41.0 20-21 35.38585580078875 39.0 35.0 41.0 26.0 41.0 22-23 35.078545592750416 39.0 35.0 41.0 24.5 41.0 24-25 34.83963092055636 39.0 34.0 41.0 24.0 41.0 26-27 33.89172610933923 38.0 33.0 40.0 23.0 40.5 28-29 33.90362594788455 38.0 33.0 40.0 22.5 40.5 30-31 34.3182489280468 38.0 34.0 40.0 23.0 41.0 32-33 34.20990121653455 38.0 34.0 40.0 22.0 41.0 34-35 33.97046090679301 38.0 33.5 40.0 21.5 41.0 36-37 33.67808525667324 38.0 33.0 40.0 20.0 41.0 38-39 33.37736578252584 37.0 33.0 40.0 18.5 41.0 40-41 33.029888139698 37.0 32.5 40.0 17.0 41.0 42-43 32.67030863319349 36.0 32.0 39.5 13.5 41.0 44-45 32.3089632161243 36.0 32.0 39.0 12.0 41.0 46-47 31.89708546180009 35.0 31.0 39.0 8.0 40.0 48-49 31.476085559400765 35.0 31.0 38.0 6.5 40.0 50-51 31.012074155009728 35.0 31.0 37.0 2.0 39.0 52-53 30.525334819951564 35.0 30.0 37.0 2.0 39.0 54-55 30.058071782816988 34.5 30.0 36.0 2.0 39.0 56-57 29.608425461204558 34.0 29.5 36.0 2.0 37.5 58-59 29.179725651112314 34.0 29.0 35.0 2.0 37.0 60-61 28.814104414528273 34.0 29.0 35.0 2.0 36.5 62-63 28.44452497419372 34.0 29.0 35.0 2.0 36.0 64-65 28.067107082831544 34.0 28.5 35.0 2.0 36.0 66-67 27.715439227862845 34.0 27.0 35.0 2.0 35.0 68-69 27.353039640763335 34.0 26.5 35.0 2.0 35.0 70-71 26.91263912232177 33.0 25.5 35.0 2.0 35.0 72-73 26.44796994726255 33.0 25.0 35.0 2.0 35.0 74-75 25.891630990034805 33.0 24.0 35.0 2.0 35.0 76-77 24.16357625425142 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7307192490960048 1101 2 0.7070410833835581 1101 3 0.7670216168726114 1101 4 0.6605720528600116 1101 5 0.951938019946347 1101 6 0.8487592429661603 1101 7 0.8165937274738369 1101 8 0.7477800901456888 1101 9 0.8166723378106724 1101 10-11 0.847712987824103 1101 12-13 0.9816413206383885 1101 14-15 0.9401818554455517 1101 16-17 0.8895614163937964 1101 18-19 0.888482369509795 1101 20-21 0.8845531065510812 1101 22-23 0.8909365720397986 1101 24-25 1.0849212953148637 1101 26-27 0.9381353788049651 1101 28-29 0.9116436306265641 1101 30-31 0.9507015506979712 1101 32-33 0.8619214336634897 1101 34-35 0.9295462370684078 1101 36-37 1.0606143100792664 1101 38-39 0.9007102845444734 1101 40-41 0.8003724562540526 1101 42-43 0.7962164634030202 1101 44-45 0.7964948812347572 1101 46-47 0.8007358361963135 1101 48-49 0.7393099182139942 1101 50-51 0.6442862030116707 1101 52-53 0.6477929432341725 1101 54-55 0.7615540703200239 1101 56-57 0.7003567677311828 1101 58-59 0.7988321858546144 1101 60-61 0.79152671705911 1101 62-63 0.8953727652377808 1101 64-65 0.733364618644206 1101 66-67 0.8200348643280151 1101 68-69 0.7673463467878534 1101 70-71 0.8523362093731102 1101 72-73 0.769767568956933 1101 74-75 0.6292352928021145 1101 76-77 1.0210062650806506 1102 1 1.1010413422496583 1102 2 0.999215928042176 1102 3 1.0578169799854678 1102 4 0.830529840942468 1102 5 0.8582911476240653 1102 6 0.8928218651134259 1102 7 0.8013632495330256 1102 8 0.9172366049024419 1102 9 1.0072705232534958 1102 10-11 1.1014403643811974 1102 12-13 1.144658258755527 1102 14-15 1.117005150065573 1102 16-17 1.1414413546818665 1102 18-19 1.0302414643434403 1102 20-21 1.1012392804876967 1102 22-23 1.0921478374065359 1102 24-25 1.1736098732729943 1102 26-27 1.02304121585194 1102 28-29 1.0396218463591893 1102 30-31 1.123629742531918 1102 32-33 1.1624600520697186 1102 34-35 1.012755758213018 1102 36-37 1.0912064636927568 1102 38-39 1.2672046702614495 1102 40-41 1.2460880609449418 1102 42-43 1.2456902026254824 1102 44-45 1.1646674002638733 1102 46-47 1.1674800964316425 1102 48-49 1.128575112925585 1102 50-51 1.2410580855412512 1102 52-53 1.1936769818596566 1102 54-55 1.1474925042872783 1102 56-57 1.2476317200699292 1102 58-59 1.3573337444401155 1102 60-61 1.2745954592267132 1102 62-63 1.2203211299478767 1102 64-65 1.1005127065869722 1102 66-67 1.0780968832923463 1102 68-69 1.13577385759125 1102 70-71 1.0635814337212324 1102 72-73 1.0851457940530445 1102 74-75 1.2300143133593302 1102 76-77 1.3167000397754975 1103 1 0.3890276047017167 1103 2 0.3706101952413263 1103 3 0.28920714897267885 1103 4 0.3507579320508398 1103 5 0.2782415421836859 1103 6 0.3827607547968199 1103 7 0.3582156946927597 1103 8 0.2815635909877301 1103 9 0.3795514556820905 1103 10-11 0.3032892556227651 1103 12-13 0.3883889550033075 1103 14-15 0.6351149778766327 1103 16-17 0.5394187163298696 1103 18-19 0.5139635586151172 1103 20-21 0.5943473473483181 1103 22-23 0.484959530971274 1103 24-25 0.6321715076057615 1103 26-27 0.6485443674196105 1103 28-29 0.5618295659390427 1103 30-31 0.6554200208047831 1103 32-33 0.7531436637043711 1103 34-35 0.6471004818718669 1103 36-37 0.6613181186233135 1103 38-39 0.4992843847526842 1103 40-41 0.640763373700409 1103 42-43 0.5422679822057077 1103 44-45 0.4794181884226063 1103 46-47 0.5456541295631219 1103 48-49 0.4456645499977476 1103 50-51 0.5337634817337538 1103 52-53 0.5858936876229812 1103 54-55 0.5995635954575533 1103 56-57 0.7468864644973223 1103 58-59 0.5446165872331328 1103 60-61 0.43819501269237193 1103 62-63 0.5637517175503568 1103 64-65 0.5430429682535376 1103 66-67 0.3380856345543606 1103 68-69 0.38389102803204267 1103 70-71 0.32229440306788604 1103 72-73 0.2860111144286286 1103 74-75 0.23247601535528517 1103 76-77 0.2663386713996019 1104 1 0.549847749436033 1104 2 0.590303065280537 1104 3 0.61086506696968 1104 4 0.7083526300454537 1104 5 0.5581579747598937 1104 6 0.48770215428879027 1104 7 0.39844440639990353 1104 8 0.440390072153086 1104 9 0.3967101632731769 1104 10-11 0.5974515889511736 1104 12-13 0.7365167232972141 1104 14-15 0.7616246768469566 1104 16-17 0.7035847967454671 1104 18-19 0.6103604514025065 1104 20-21 0.5978502999218378 1104 22-23 0.5940608531929001 1104 24-25 0.6114735654699643 1104 26-27 0.4718427634695104 1104 28-29 0.4322098665915419 1104 30-31 0.4973743194599223 1104 32-33 0.5269292630825291 1104 34-35 0.6088441155133353 1104 36-37 0.6409795114902295 1104 38-39 0.6441906829749087 1104 40-41 0.7234346123054181 1104 42-43 0.7330869626372234 1104 44-45 0.49669599107163975 1104 46-47 0.34684450619048235 1104 48-49 0.42147000975797155 1104 50-51 0.35699054373861827 1104 52-53 0.42187478492097696 1104 54-55 0.38731160425378164 1104 56-57 0.4624103925598071 1104 58-59 0.3479076284970084 1104 60-61 0.4865929074361155 1104 62-63 0.44290323598159986 1104 64-65 0.37266324155312347 1104 66-67 0.45252655602623904 1104 68-69 0.3155327195836328 1104 70-71 0.3127098131894499 1104 72-73 0.33431615192409936 1104 74-75 0.47893083630683364 1104 76-77 0.4980908897244163 1105 1 -0.006135901533369292 1105 2 0.1797928059104663 1105 3 0.14670768595560446 1105 4 0.2264311870589637 1105 5 0.06433793973747726 1105 6 0.10194826923456901 1105 7 0.10831154107916774 1105 8 0.2037979262839258 1105 9 0.05881135136555571 1105 10-11 0.12901377730268138 1105 12-13 0.23955998305205384 1105 14-15 0.12652608795205111 1105 16-17 0.012466631417666463 1105 18-19 -0.09202005226071464 1105 20-21 0.05346389898581805 1105 22-23 -0.045474432286930266 1105 24-25 -0.21432209520519052 1105 26-27 -0.3051168429601674 1105 28-29 -0.17087776012915157 1105 30-31 5.689373150801202E-4 1105 32-33 -0.092193095512485 1105 34-35 -0.13885508431076943 1105 36-37 -0.07655113312644346 1105 38-39 -0.07705108165244923 1105 40-41 -0.051134871074637545 1105 42-43 -0.021677970969371074 1105 44-45 -0.252922570810469 1105 46-47 -0.3625734620380605 1105 48-49 -0.4370861399746495 1105 50-51 -0.4846136950734454 1105 52-53 -0.33240466414338954 1105 54-55 -0.5654846924787691 1105 56-57 -0.46763193274035686 1105 58-59 -0.4605868786370415 1105 60-61 -0.266222431755228 1105 62-63 -0.40656082677606165 1105 64-65 -0.13004628438781296 1105 66-67 -0.24417792071840694 1105 68-69 -0.29159053420326586 1105 70-71 -0.5046580871144428 1105 72-73 -0.7844730617990372 1105 74-75 -0.8008702190772752 1105 76-77 -0.8557633431982268 1106 1 0.019158504040902358 1106 2 -0.05700333202585739 1106 3 -0.1753005103723666 1106 4 0.03476380057426809 1106 5 0.10101794703163591 1106 6 -0.2093269603873864 1106 7 -0.3042987110490927 1106 8 -0.1442801595759704 1106 9 -0.10944947229822333 1106 10-11 -0.38114813376619594 1106 12-13 -0.0296250869622412 1106 14-15 -0.019678551515092124 1106 16-17 -0.07511890695823098 1106 18-19 0.13082985128367142 1106 20-21 0.02270175565564614 1106 22-23 -0.1241308679400035 1106 24-25 -0.25588630777225774 1106 26-27 -0.23467832981628334 1106 28-29 0.10078780281653366 1106 30-31 0.4424277953957585 1106 32-33 0.2397783421080888 1106 34-35 0.12936303014989647 1106 36-37 0.043945445338522404 1106 38-39 0.146869431241889 1106 40-41 -0.07317857842542708 1106 42-43 0.07833354611910437 1106 44-45 -0.039860752838045244 1106 46-47 -0.021109544116509227 1106 48-49 -0.06475498529055201 1106 50-51 -0.15692805400351162 1106 52-53 -0.08941953078510423 1106 54-55 -0.00579594179494336 1106 56-57 0.018728475348478213 1106 58-59 -0.21286678227739841 1106 60-61 -0.6153332165488301 1106 62-63 -0.4386277602552795 1106 64-65 -0.2686419673991445 1106 66-67 -0.42712783098108886 1106 68-69 -0.5147950680970972 1106 70-71 -0.4236934349785031 1106 72-73 -0.373933006325057 1106 74-75 -0.2804720157430758 1106 76-77 -0.27630503025973496 1107 1 0.07513422766368905 1107 2 0.00829389797725355 1107 3 0.10316198573718793 1107 4 0.00876006574700483 1107 5 0.19988193707209234 1107 6 -0.33883209577486895 1107 7 -0.20636842782469245 1107 8 -0.11052665817540941 1107 9 -0.12291041222974997 1107 10-11 -0.1293118436946159 1107 12-13 -0.11213364594139108 1107 14-15 -0.2540934285770291 1107 16-17 -0.1531378923301574 1107 18-19 -0.1332224362980199 1107 20-21 -0.23201346376545473 1107 22-23 -0.18374026725528125 1107 24-25 -0.13428655676074186 1107 26-27 -0.33134032794887247 1107 28-29 -0.24067656070665322 1107 30-31 -0.11905679844178252 1107 32-33 0.06038992007260191 1107 34-35 -0.060171672620107586 1107 36-37 0.05784989600768142 1107 38-39 0.11683519532530084 1107 40-41 0.1207990126109948 1107 42-43 0.13290870951779965 1107 44-45 0.07741282325905985 1107 46-47 -0.07551352793226229 1107 48-49 0.02236764153795434 1107 50-51 0.07133991985270072 1107 52-53 0.14913477982803158 1107 54-55 0.07037119172512973 1107 56-57 0.01941319368025418 1107 58-59 -0.033789595847270704 1107 60-61 -0.1100966774887624 1107 62-63 -0.04812044621855094 1107 64-65 -0.03439048903002018 1107 66-67 -0.09200489291697878 1107 68-69 -0.2142037204469247 1107 70-71 -0.40407781095111517 1107 72-73 -0.29676991886785586 1107 74-75 -0.330121875687059 1107 76-77 -0.1350196272096298 1108 1 0.2524699082534383 1108 2 0.2745587345174343 1108 3 0.31016868367004236 1108 4 0.13044857748558059 1108 5 0.09771645549484731 1108 6 0.3423813716088091 1108 7 0.4137673187816375 1108 8 0.31271736938651173 1108 9 0.20119114328607424 1108 10-11 0.2696036963348192 1108 12-13 0.3461201465739805 1108 14-15 0.3326815923969946 1108 16-17 0.2621796749586238 1108 18-19 0.18546197691079414 1108 20-21 0.2700392394762332 1108 22-23 0.1660579007844376 1108 24-25 0.22510774105658982 1108 26-27 0.14854455562812774 1108 28-29 0.19516494447152155 1108 30-31 0.33909590617525964 1108 32-33 0.33980615465154784 1108 34-35 0.3365219966643096 1108 36-37 0.36962560465447325 1108 38-39 0.4471498743884226 1108 40-41 0.2221513211285071 1108 42-43 0.3784464008627495 1108 44-45 0.30066132069404006 1108 46-47 0.2948371164171384 1108 48-49 0.3180693428404062 1108 50-51 0.2344404301059022 1108 52-53 0.36064367143704956 1108 54-55 0.3819213437008173 1108 56-57 0.3431667867705208 1108 58-59 0.4383094565396384 1108 60-61 0.46030932451924045 1108 62-63 0.4453002444799843 1108 64-65 0.46691093170193554 1108 66-67 0.6170923302603448 1108 68-69 0.6678404556523176 1108 70-71 0.615507453596603 1108 72-73 0.7480876640998666 1108 74-75 0.5354164345633947 1108 76-77 0.5826178041950101 1109 1 0.056509086885725424 1109 2 -0.008814306129131921 1109 3 0.058520211501722486 1109 4 0.0020867572483496133 1109 5 0.12970693923790577 1109 6 -0.05332399562759349 1109 7 0.050896581271729247 1109 8 0.13270387114972237 1109 9 0.1250450586183831 1109 10-11 -0.15003522583096895 1109 12-13 0.09002004027536259 1109 14-15 0.24122884466216732 1109 16-17 0.2917018373761593 1109 18-19 0.26642583538178144 1109 20-21 0.281664089730171 1109 22-23 0.22334931084400012 1109 24-25 -0.019813716078076027 1109 26-27 -0.09411906831099515 1109 28-29 0.1057528770478342 1109 30-31 0.18424303695629618 1109 32-33 0.24979518733415063 1109 34-35 0.3747018437967711 1109 36-37 0.31348837820468134 1109 38-39 0.3821509711201543 1109 40-41 0.479479598247309 1109 42-43 0.490307132804638 1109 44-45 0.40553376235634886 1109 46-47 0.5557729660467707 1109 48-49 0.4942540662109174 1109 50-51 0.2781652685488787 1109 52-53 0.3563109905184909 1109 54-55 0.3477570532862515 1109 56-57 0.4365421896085273 1109 58-59 0.476612812538761 1109 60-61 0.41942814274861107 1109 62-63 0.29608643240918653 1109 64-65 0.37202465434691945 1109 66-67 0.49401000788870775 1109 68-69 0.38916108939453764 1109 70-71 0.23202069030505612 1109 72-73 0.28083598947486266 1109 74-75 0.20838587792985308 1109 76-77 0.3964479288849745 1110 1 -0.2164438546733507 1110 2 -0.12867625285910833 1110 3 -0.3304428442074112 1110 4 -0.43983363040653245 1110 5 -0.3391313969681491 1110 6 -0.3396320056441837 1110 7 -0.2825745885224151 1110 8 -0.5503697247395749 1110 9 -0.49835245024369357 1110 10-11 -0.41963041591845496 1110 12-13 -0.5023786556171927 1110 14-15 -0.3278673861095669 1110 16-17 -0.48234951388729996 1110 18-19 -0.5801560046780594 1110 20-21 -0.6782216789127133 1110 22-23 -0.6650626700334286 1110 24-25 -0.6051060833095363 1110 26-27 -0.6096894120620178 1110 28-29 -0.4378702489074797 1110 30-31 -0.2950528751058812 1110 32-33 -0.3036346803038228 1110 34-35 -0.3635220821845593 1110 36-37 -0.3091844729449491 1110 38-39 -0.39777260363023714 1110 40-41 -0.2806699188101547 1110 42-43 -0.3502421533510116 1110 44-45 -0.23820099324546362 1110 46-47 -0.24818106497862402 1110 48-49 -0.29142367940682945 1110 50-51 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-0.49496759225737463 2314 22-23 -0.3908694278462974 2314 24-25 -0.39470450528418155 2314 26-27 -0.3625724697524362 2314 28-29 -0.5866564471446765 2314 30-31 -0.6829797999392682 2314 32-33 -0.7839018487914089 2314 34-35 -0.7046033570290717 2314 36-37 -1.3012708134596593 2314 38-39 -0.8359992182796603 2314 40-41 -0.706917048738319 2314 42-43 -0.6336330726915733 2314 44-45 -0.6027009072883303 2314 46-47 -0.4772489864085294 2314 48-49 -0.3035522048358992 2314 50-51 -0.1760173480293794 2314 52-53 -0.09071579430332477 2314 54-55 -0.2445796130702078 2314 56-57 -0.2970985263537962 2314 58-59 -0.2975550518792325 2314 60-61 -0.05881180044867662 2314 62-63 -0.24133173273918018 2314 64-65 -0.26067929943883783 2314 66-67 -0.22264400914521687 2314 68-69 -0.1210312057038152 2314 70-71 -0.07301241588636742 2314 72-73 0.02930262649934079 2314 74-75 0.022216441786373053 2314 76-77 -0.06138991454221454 2315 1 -1.0130165178234734 2315 2 -1.0146810121093566 2315 3 -1.0476249889096678 2315 4 -0.8771537890696948 2315 5 -0.9600478775503873 2315 6 -0.9331654777487017 2315 7 -1.3828199890416215 2315 8 -1.3260182276326091 2315 9 -0.9604570141187168 2315 10-11 -0.9873838774110624 2315 12-13 -0.9740673060244234 2315 14-15 -0.9616924334496559 2315 16-17 -1.0239157540366435 2315 18-19 -0.9890056484592904 2315 20-21 -0.9964661294078141 2315 22-23 -0.9193732231955707 2315 24-25 -1.128166723740712 2315 26-27 -1.033086736420337 2315 28-29 -1.0123263748713356 2315 30-31 -1.0176595286043266 2315 32-33 -0.9644810395472021 2315 34-35 -1.0997621053202522 2315 36-37 -1.1192825606522092 2315 38-39 -1.0516348893377696 2315 40-41 -1.2186831949809296 2315 42-43 -1.2818192041195644 2315 44-45 -1.306772434092096 2315 46-47 -1.1563900869317543 2315 48-49 -1.1329322414387306 2315 50-51 -1.004590401203199 2315 52-53 -1.0957327177327905 2315 54-55 -1.1681070902924766 2315 56-57 -1.4116157245264525 2315 58-59 -1.267801678719234 2315 60-61 -1.2106496770338318 2315 62-63 -1.1318579248668037 2315 64-65 -1.1404503797891827 2315 66-67 -1.0876279633913377 2315 68-69 -1.2676979679927243 2315 70-71 -1.2125660785768595 2315 72-73 -1.0995449308133871 2315 74-75 -1.1837526742781996 2315 76-77 -1.2374861296430844 2316 1 -2.0929532537950095 2316 2 -1.5667914605590596 2316 3 -1.9950147985684126 2316 4 -2.7951082521491983 2316 5 -2.3462989346966907 2316 6 -2.2281449938228164 2316 7 -1.8436698797574067 2316 8 -1.555526064244475 2316 9 -1.9720773860145542 2316 10-11 -1.8748101517528823 2316 12-13 -2.424010741832305 2316 14-15 -3.6817216491294715 2316 16-17 -2.7953305178399503 2316 18-19 -2.3153920203331566 2316 20-21 -1.905919488661823 2316 22-23 -2.3659612491601862 2316 24-25 -2.241921158263537 2316 26-27 -2.150416247551945 2316 28-29 -2.8057956819325263 2316 30-31 -2.903493218896152 2316 32-33 -2.7784836975150604 2316 34-35 -2.7935905675430597 2316 36-37 -2.83669774182237 2316 38-39 -2.946041075153893 2316 40-41 -2.6762984925744817 2316 42-43 -2.8792578896336423 2316 44-45 -2.6580086088150416 2316 46-47 -2.500585941819889 2316 48-49 -2.4854369917325343 2316 50-51 -2.508950786498705 2316 52-53 -2.605941730660181 2316 54-55 -2.5834694855388847 2316 56-57 -2.5112071443425954 2316 58-59 -2.767971920462511 2316 60-61 -2.4501633348149774 2316 62-63 -2.360250952643664 2316 64-65 -2.3708972918236277 2316 66-67 -2.315587149306303 2316 68-69 -2.386971529999162 2316 70-71 -2.473799287798471 2316 72-73 -2.3364610290922982 2316 74-75 -2.610460857963549 2316 76-77 -2.975698261838545 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57850.0 3 5140.0 4 6962.0 5 8803.0 6 9601.0 7 10419.0 8 12097.0 9 13148.0 10 16845.0 11 19115.0 12 14249.0 13 11513.0 14 11214.0 15 11519.0 16 11828.0 17 12643.0 18 13239.0 19 13839.0 20 14897.0 21 16072.0 22 17710.0 23 20196.0 24 23388.0 25 27290.0 26 32015.0 27 37685.0 28 44387.0 29 52184.0 30 62005.0 31 74978.0 32 90611.0 33 113135.0 34 151857.0 35 200637.0 36 285583.0 37 403352.0 38 385845.0 39 103338.0 40 827.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.098934998558686 27.00527847982428 29.323334702272312 21.57245181934472 2 21.80575954060659 27.17858447173191 29.280911507459063 21.73474448020243 3 22.085716259237522 27.287722611500666 28.925049664539458 21.701511464722355 4 21.63023771714766 27.20827023536765 29.345404020703715 21.816088026780974 5 21.98705940406086 27.37701656517058 29.18384406759751 21.452079963171045 6 22.01016427711987 27.238186313093983 29.262521182470984 21.48912822731516 7 22.007485964333917 26.94190208352033 29.16680282148635 21.883809130659404 8 21.724811171686923 27.357024082331627 28.965270481563905 21.952894264417548 9 21.869144420462565 26.92819968513786 29.218974787037872 21.9836811073617 10-11 22.14190455862376 27.213148049790608 29.007658375401864 21.637289016183768 12-13 22.220484886315152 26.918344591890488 28.9015962600949 21.95957426169946 14-15 21.931464625419157 27.272616353072678 29.0395100509657 21.756408970542466 16-17 22.135524964281498 27.12277714487309 29.052393134520155 21.68930475632526 18-19 22.11255931227541 26.980860416790755 28.80405110286089 22.102529168072955 20-21 22.108252669526347 27.227162764072894 28.674065278020773 21.990519288379986 22-23 22.21906028975664 27.25184109607286 28.302634438945763 22.226464175224738 24-25 22.168952298023854 27.529274041012098 28.033564188822346 22.268209472141702 26-27 22.279901508843814 27.50574000932299 27.921386586060432 22.292971895772766 28-29 22.36233082293169 27.550275931867183 27.917116626761356 22.170276618439768 30-31 22.162785912443404 27.41421721952637 28.06098086799714 22.362016000033087 32-33 22.25554732710623 27.67280588031046 27.859176466184554 22.212470326398755 34-35 22.29949390585888 27.315579228797898 28.01894502781946 22.365981837523762 36-37 22.3394427774971 27.52000354046794 28.122565540534534 22.017988141500428 38-39 22.263804113649755 27.440001604907042 28.179975434167687 22.116218847275515 40-41 22.08268430104289 27.390534482744357 28.160273681371816 22.366507534840935 42-43 22.045579151549344 27.676678516482234 27.88709219423991 22.390650137728514 44-45 22.171725966790497 27.38795422328537 28.080449676347357 22.359870133576774 46-47 22.028209072585064 27.38231983821912 28.13550761894795 22.453963470247864 48-49 22.161437872946692 27.376885324185075 27.947066051001084 22.51461075186715 50-51 21.99810326942985 27.5252630239925 27.887296559099084 22.58933714747856 52-53 21.9078930221648 27.3838940828914 28.107930999817558 22.600281895126244 54-55 21.89391715898982 27.368844305918984 28.031094180834465 22.70614435425673 56-57 21.853653512506384 27.547029099420072 27.929946139300732 22.669371248772816 58-59 22.08962663153519 27.481226099599393 27.855690699603112 22.5734565692623 60-61 21.940616986200055 27.312240138042892 27.85139003590265 22.895752839854406 62-63 21.883355537202288 27.25702276211993 28.014829330121582 22.844792370556203 64-65 21.956549076189066 27.132856198546758 28.037122035039708 22.87347269022447 66-67 21.779011553618577 27.212947531572762 28.18049225008697 22.827548664721693 68-69 21.90981522341004 27.102759083023603 28.142754400055765 22.844671293510594 70-71 21.646961215658568 26.921428889167053 28.235157807403482 23.196452087770897 72-73 21.812060677919106 26.773086847392673 28.17318575629096 23.241666718397262 74-75 21.670534516576705 26.83952582519305 28.030653900485543 23.459285757744702 76-77 21.513785512975957 26.649101470257435 28.432730450596537 23.404382566170067 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 12824.0 1 7522.5 2 2028.5 3 1837.0 4 1838.0 5 1885.0 6 1921.0 7 1994.0 8 2132.5 9 2187.0 10 2263.5 11 2387.0 12 2523.5 13 2613.0 14 2796.0 15 3074.5 16 3441.0 17 3712.0 18 4039.0 19 4804.5 20 5758.5 21 7220.5 22 8167.0 23 9250.0 24 11842.0 25 15555.5 26 17760.0 27 20711.0 28 27481.5 29 34747.0 30 38193.0 31 43527.5 32 54205.0 33 64554.5 34 74285.5 35 79010.0 36 84531.5 37 94572.5 38 99752.0 39 100412.0 40 102433.0 41 105594.0 42 110780.5 43 114827.0 44 114437.0 45 114348.5 46 115609.5 47 118577.5 48 120586.0 49 118840.0 50 115047.5 51 108236.5 52 103472.0 53 100022.0 54 91697.0 55 80418.0 56 68594.5 57 63175.0 58 59217.5 59 49785.0 60 40149.0 61 35988.0 62 31036.5 63 21791.5 64 14923.0 65 12348.0 66 10596.5 67 7638.0 68 5546.0 69 3851.5 70 3042.0 71 2565.5 72 1859.5 73 1496.0 74 1362.0 75 1245.5 76 876.0 77 484.5 78 346.0 79 295.5 80 213.5 81 158.0 82 108.5 83 83.0 84 76.5 85 59.0 86 49.5 87 51.0 88 43.5 89 30.5 90 26.0 91 27.0 92 24.0 93 19.0 94 17.0 95 16.0 96 15.0 97 18.0 98 22.0 99 49.0 100 75.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0035152786416632474 2 0.009057012029697074 3 0.012200084697537155 4 0.019809629051255245 5 0.0145987454177309 6 0.013771621031457196 7 0.01757639320831624 8 0.019685560393314188 9 0.01861029869115837 10-11 0.02200150867488056 12-13 0.02156726837208687 14-15 0.008829552823471804 16-17 0.006265467226023318 18-19 0.012572290671360321 20-21 0.010959398118126596 22-23 0.01515705437846565 24-25 0.023097448486693224 26-27 0.013937045908711936 28-29 0.008643449836560221 30-31 0.008622771726903379 32-33 0.010442445376705531 34-35 0.011745166285086617 36-37 0.010607870253960271 38-39 0.017803852414541507 40-41 0.012985852864497174 42-43 0.016439097177189894 44-45 0.018817079787726797 46-47 0.01757639320831624 48-49 0.014950273281897226 50-51 0.02888731919060916 52-53 0.03329175654751664 54-55 0.026902220663552266 56-57 0.016149603641994095 58-59 0.017824530524198353 60-61 0.02094692508238159 62-63 0.026654083347670155 64-65 0.02191879623625319 66-67 0.020884890753411062 68-69 0.03083106149835237 70-71 0.057216329420483575 72-73 0.06827911808689438 74-75 0.08043784656511785 76-77 0.07752223310350304 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2418016.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.52808117778418 #Duplication Level Percentage of deduplicated Percentage of total 1 74.4885059110648 39.87226791219334 2 13.250265185488583 14.185225409520008 3 5.050202879711639 8.109830091284522 4 2.442744075770971 5.2302161273767975 5 1.3658225340683572 3.6554929739028963 6 0.8094587028272994 2.5997262693002137 7 0.5396174642370142 2.0219281201440262 8 0.3731082472189199 1.5977414836188093 9 0.26062678423018637 1.255576649704043 >10 1.2723090117225175 12.300286153017266 >50 0.09966112720491704 3.732013935484674 >100 0.04628258934441796 4.3677822002589695 >500 0.0012404329870670497 0.38971474732377953 >1k 7.752706169151277E-5 0.20546030390758666 >5k 0.0 0.0 >10k+ 7.752706169151277E-5 0.4767376229631097 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11488 0.4751002474756164 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4951 0.20475464182205574 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 8.271243862737054E-5 0.0 2 0.0 0.0 0.0 5.376308510779085E-4 0.0 3 0.0 0.0 0.0 7.857681669600201E-4 0.0 4 4.135621931368527E-5 0.0 0.0 0.0013647552373516139 0.0 5 8.271243862737054E-5 0.0 0.0 0.0026881542553895423 0.0 6 8.271243862737054E-5 0.0 0.0 0.005500377168720141 0.0 7 8.271243862737054E-5 0.0 0.0 0.010959398118126596 0.0 8 8.271243862737054E-5 0.0 0.0 0.017080118576552016 0.0 9 8.271243862737054E-5 0.0 0.0 0.02324219525429112 0.0 10 8.271243862737054E-5 0.0 0.0 0.0303141087569313 0.0 11 8.271243862737054E-5 0.0 0.0 0.03899891481280521 0.0 12 8.271243862737054E-5 0.0 0.0 0.0502478064661276 0.0 13 8.271243862737054E-5 0.0 0.0 0.06699707528817013 0.0 14 8.271243862737054E-5 0.0 0.0 0.08808874713814963 0.0 15 8.271243862737054E-5 0.0 0.0 0.11029703690959862 0.0 16 8.271243862737054E-5 0.0 0.0 0.13316702619006657 0.0 17 8.271243862737054E-5 0.0 0.0 0.15343157365377236 0.0 18 8.271243862737054E-5 0.0 0.0 0.17642563159218136 0.0 19 8.271243862737054E-5 0.0 0.0 0.19999867660098197 0.0 20 8.271243862737054E-5 0.0 0.0 0.22816226195360162 0.0 21 8.271243862737054E-5 4.135621931368527E-5 0.0 0.2569048343766129 0.0 22 8.271243862737054E-5 4.135621931368527E-5 0.0 0.29040337202069794 0.0 23 8.271243862737054E-5 4.135621931368527E-5 0.0 0.32514259624419356 0.0 24 1.240686579410558E-4 4.135621931368527E-5 0.0 0.35950961449386604 0.0 25 1.240686579410558E-4 4.135621931368527E-5 0.0 0.3970197054113786 0.0 26 1.240686579410558E-4 4.135621931368527E-5 0.0 0.4355223455924196 0.0 27 1.240686579410558E-4 4.135621931368527E-5 0.0 0.4729910802906184 0.0 28 1.240686579410558E-4 4.135621931368527E-5 0.0 0.5117418577875416 0.0 29 1.240686579410558E-4 4.135621931368527E-5 0.0 0.5502444979685825 0.0 30 1.240686579410558E-4 4.135621931368527E-5 0.0 0.5928414038616784 0.0 31 1.240686579410558E-4 4.135621931368527E-5 0.0 0.6379196829135952 0.0 32 1.6542487725474108E-4 4.135621931368527E-5 0.0 0.6882088455990366 0.0 33 1.6542487725474108E-4 4.135621931368527E-5 0.0 0.7437502481373158 0.0 34 1.6542487725474108E-4 4.135621931368527E-5 0.0 0.7990848695790268 0.0 35 1.6542487725474108E-4 4.135621931368527E-5 0.0 0.8581415507589694 0.0 36 1.6542487725474108E-4 4.135621931368527E-5 0.0 0.9210443603350846 0.0 37 1.6542487725474108E-4 4.135621931368527E-5 0.0 0.986966173921099 0.0 38 1.6542487725474108E-4 4.135621931368527E-5 0.0 1.0530120561650542 0.0 39 1.6542487725474108E-4 4.135621931368527E-5 0.0 1.1203813374270477 0.0 40 1.6542487725474108E-4 4.135621931368527E-5 0.0 1.1906869102603126 0.0 41 1.6542487725474108E-4 4.135621931368527E-5 0.0 1.2652935299022008 0.0 42 2.0678109656842634E-4 4.135621931368527E-5 0.0 1.3473442690205524 0.0 43 2.0678109656842634E-4 4.135621931368527E-5 0.0 1.4341509733599778 0.0 44 2.0678109656842634E-4 4.135621931368527E-5 0.0 1.5298906210711591 0.0 45 2.0678109656842634E-4 4.135621931368527E-5 0.0 1.6272431613355742 0.0 46 2.0678109656842634E-4 4.135621931368527E-5 0.0 1.740807339570954 0.0 47 2.0678109656842634E-4 4.135621931368527E-5 0.0 1.851559294893003 0.0 48 2.0678109656842634E-4 4.135621931368527E-5 0.0 1.9637173616717176 0.0 49 2.0678109656842634E-4 4.135621931368527E-5 0.0 2.081954792689544 0.0 50 2.0678109656842634E-4 4.135621931368527E-5 0.0 2.198910180908646 0.0 51 2.0678109656842634E-4 4.135621931368527E-5 0.0 2.3267836110265607 0.0 52 2.0678109656842634E-4 4.135621931368527E-5 0.0 2.459330293926922 0.0 53 2.0678109656842634E-4 4.135621931368527E-5 0.0 2.600975345076294 0.0 54 2.0678109656842634E-4 4.135621931368527E-5 0.0 2.7541587814141844 0.0 55 2.0678109656842634E-4 4.135621931368527E-5 0.0 2.9156548178341253 0.0 56 2.894935351957969E-4 4.135621931368527E-5 0.0 3.0914601061366014 0.0 57 2.894935351957969E-4 4.135621931368527E-5 0.0 3.2700362611330944 0.0 58 2.894935351957969E-4 4.135621931368527E-5 0.0 3.4458001932162565 0.0 59 2.894935351957969E-4 4.135621931368527E-5 0.0 3.6279743392930404 0.0 60 2.894935351957969E-4 4.135621931368527E-5 0.0 3.815111231687466 0.0 61 3.722059738231674E-4 4.135621931368527E-5 0.0 4.008865119172081 0.0 62 3.722059738231674E-4 4.135621931368527E-5 0.0 4.211345168931885 0.0 63 3.722059738231674E-4 4.135621931368527E-5 0.0 4.423461217791776 0.0 64 3.722059738231674E-4 4.135621931368527E-5 0.0 4.653153659859984 0.0 65 3.722059738231674E-4 4.135621931368527E-5 0.0 4.8872298611754434 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTATA 155 3.0235242E-8 22.902498 10 TCGCGCT 155 7.653816E-7 20.60969 37 TCGCGGA 160 1.0351741E-6 19.971422 58 AACTCGA 205 2.7645001E-8 19.045418 13 CGCGGAA 150 1.3230161E-5 18.937042 59 CCTCGCG 220 6.209666E-8 17.745766 35 ACGTAAT 375 0.0 17.035582 32 ATACGTA 255 1.834269E-8 16.702587 1 AACGCTC 320 6.366463E-11 16.637 15 GACGTAA 385 0.0 16.593443 31 CTCGCGC 215 9.49507E-7 16.50803 36 TAATTCG 390 0.0 16.380707 35 GACGAAC 285 4.107278E-9 16.191797 38 AGTACGC 180 6.3433705E-5 15.776625 38 CGCGCTT 205 1.1024902E-5 15.584227 38 ACGAGAC 460 0.0 15.43078 27 ACGTATG 140 0.0034189075 15.213174 38 CGATCGT 210 1.3867708E-5 15.211286 1 CGCCGTA 145 0.0043022283 14.6964855 67 CGTAACG 145 0.0043247645 14.684935 28 >>END_MODULE