FastQCFastQC Report
Thu 26 May 2016
SRR936106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936106_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418016
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90350.3736534414991464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65410.2705110305308153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50240.2077736458319548No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12950.050.561893100-101
CGGTATC11200.039.86058100-101
CGAGTAC10600.039.30905100-101
CGTACAT17550.038.835487100-101
CCGTATC5400.037.47879100-101
TCGTATC5100.037.349102100-101
ACCGTAC2950.037.32584898-99
TACGCAG10150.036.360184100-101
ACGTATC3950.036.16717100-101
CCGTACA8600.034.953716100-101
GGTATCA92500.034.423071
TCGAGTA4350.032.841454100-101
CCTGTCG7350.032.7944896-97
CGGGCTA1950.030.525711100-101
GCGTATC3650.030.170273100-101
AGGTATC23650.029.82549100-101
TACGCGG504.4337753E-429.75795296-97
CTACGCG1800.029.75795296-97
TACGCGC400.00535688229.7579596-97
AGTATCA33800.029.322294100-101
Produced by FastQC (version 0.11.5)